Human Gene PDIA4 (ENST00000652332.1_11) from GENCODE V47lift37
  Description: protein disulfide isomerase family A member 4, transcript variant 8 (from RefSeq NR_163909.1)
Gencode Transcript: ENST00000652332.1_11
Gencode Gene: ENSG00000155660.11_19
Transcript (Including UTRs)
   Position: hg19 chr7:148,700,154-148,725,597 Size: 25,444 Total Exon Count: 10 Strand: -
Coding Region
   Position: hg19 chr7:148,700,886-148,725,500 Size: 24,615 Coding Exon Count: 10 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
Gene AllelesMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr7:148,700,154-148,725,597)mRNA (may differ from genome)Protein (645 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMalacardsMGIPubMedUniProtKBBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: PDIA4_HUMAN
DESCRIPTION: RecName: Full=Protein disulfide-isomerase A4; EC=5.3.4.1; AltName: Full=Endoplasmic reticulum resident protein 70; Short=ER protein 70; Short=ERp70; AltName: Full=Endoplasmic reticulum resident protein 72; Short=ER protein 72; Short=ERp-72; Short=ERp72; Flags: Precursor;
CATALYTIC ACTIVITY: Catalyzes the rearrangement of -S-S- bonds in proteins.
SUBUNIT: Part of a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX.
INTERACTION: Q76353:- (xeno); NbExp=3; IntAct=EBI-1054653, EBI-6248077;
SUBCELLULAR LOCATION: Endoplasmic reticulum lumen. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
SIMILARITY: Belongs to the protein disulfide isomerase family.
SIMILARITY: Contains 3 thioredoxin domains.
CAUTION: Was originally (PubMed:2549034) thought to be a deoxycytidine kinase.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: PDIA4
Diseases sorted by gene-association score: multiple epiphyseal dysplasia (4)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene
  • D001564 Benzo(a)pyrene
  • D019256 Cadmium Chloride
  • D002994 Clofibrate
  • D004997 Ethinyl Estradiol
  • D013749 Tetrachlorodibenzodioxin
  • C023036 perfluorooctanoic acid
  • D020122 tert-Butylhydroperoxide
  • C517041 (4-amino-1,4-dihydro-3-(2-pyridyl)-5-thioxo-1,2,4-triazole)copper(II)
  • C016403 2,4-dinitrotoluene
  • C049584 2-amino-1-methyl-6-phenylimidazo(4,5-b)pyridine
          more ... click here to view the complete list

+  Common Gene Haplotype Alleles
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+  Microarray Expression Data
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-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -40.7097-0.420 Picture PostScript Text
3' UTR -229.00732-0.313 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR005788 - Disulphide_isomerase
IPR005792 - Prot_disulphide_isomerase
IPR017068 - Protein_diS-isomerase_A4
IPR005746 - Thioredoxin
IPR012336 - Thioredoxin-like_fold
IPR017937 - Thioredoxin_CS
IPR013766 - Thioredoxin_domain

Pfam Domains:
PF00085 - Thioredoxin
PF01216 - Calsequestrin
PF13848 - Thioredoxin-like domain

SCOP Domains:
47741 - CO dehydrogenase ISP C-domain like
52833 - Thioredoxin-like
55200 - Translation initiation factor IF3, C-terminal domain
57716 - Glucocorticoid receptor-like (DNA-binding domain)

Protein Data Bank (PDB) 3-D Structure
MuPIT help
3IDV - X-ray MuPIT


ModBase Predicted Comparative 3D Structure on P13667
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
Gene SorterGene Sorter    
 RGDEnsembl   
      
      

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0003723 RNA binding
GO:0003756 protein disulfide isomerase activity
GO:0005515 protein binding
GO:0015037 peptide disulfide oxidoreductase activity
GO:0016853 isomerase activity

Biological Process:
GO:0006457 protein folding
GO:0009306 protein secretion
GO:0034976 response to endoplasmic reticulum stress
GO:0045454 cell redox homeostasis
GO:0055114 oxidation-reduction process
GO:0061077 chaperone-mediated protein folding

Cellular Component:
GO:0005615 extracellular space
GO:0005783 endoplasmic reticulum
GO:0005788 endoplasmic reticulum lumen
GO:0009986 cell surface
GO:0042470 melanosome


-  Descriptions from all associated GenBank mRNAs
  BC000425 - Homo sapiens protein disulfide isomerase family A, member 4, mRNA (cDNA clone MGC:8346 IMAGE:2819726), complete cds.
BC001928 - Homo sapiens protein disulfide isomerase family A, member 4, mRNA (cDNA clone MGC:3984 IMAGE:2819726), complete cds.
BC011754 - Homo sapiens protein disulfide isomerase family A, member 4, mRNA (cDNA clone MGC:19707 IMAGE:3532913), complete cds.
LF384104 - JP 2014500723-A/191607: Polycomb-Associated Non-Coding RNAs.
JD328509 - Sequence 309533 from Patent EP1572962.
JD266303 - Sequence 247327 from Patent EP1572962.
JD121916 - Sequence 102940 from Patent EP1572962.
JD386544 - Sequence 367568 from Patent EP1572962.
JD220580 - Sequence 201604 from Patent EP1572962.
JD487555 - Sequence 468579 from Patent EP1572962.
JD134933 - Sequence 115957 from Patent EP1572962.
JD192650 - Sequence 173674 from Patent EP1572962.
JD442378 - Sequence 423402 from Patent EP1572962.
J05016 - Human (clone pA3) protein disulfide isomerase related protein (ERp72) mRNA, complete cds.
BC006344 - Homo sapiens protein disulfide isomerase family A, member 4, mRNA (cDNA clone MGC:13117 IMAGE:4098367), complete cds.
AK290971 - Homo sapiens cDNA FLJ78033 complete cds, highly similar to Homo sapiens protein disulfide isomerase family A, member 4, mRNA.
JD082567 - Sequence 63591 from Patent EP1572962.
LF362131 - JP 2014500723-A/169634: Polycomb-Associated Non-Coding RNAs.
JD327739 - Sequence 308763 from Patent EP1572962.
HM005450 - Homo sapiens clone HTL-T-137 testicular tissue protein Li 137 mRNA, complete cds.
JD261126 - Sequence 242150 from Patent EP1572962.
JD488326 - Sequence 469350 from Patent EP1572962.
JD462192 - Sequence 443216 from Patent EP1572962.
AK313299 - Homo sapiens cDNA, FLJ93811.
KJ892814 - Synthetic construct Homo sapiens clone ccsbBroadEn_02208 PDIA4 gene, encodes complete protein.
DQ893573 - Synthetic construct clone IMAGE:100006203; FLH176704.01X; RZPDo839G05122D protein disulfide isomerase family A, member 4 (PDIA4) gene, encodes complete protein.
DQ894347 - Synthetic construct Homo sapiens clone IMAGE:100008807; FLH176700.01L; RZPDo839G05121D protein disulfide isomerase family A, member 4 (PDIA4) gene, encodes complete protein.
LF362132 - JP 2014500723-A/169635: Polycomb-Associated Non-Coding RNAs.
LF362135 - JP 2014500723-A/169638: Polycomb-Associated Non-Coding RNAs.
LF362136 - JP 2014500723-A/169639: Polycomb-Associated Non-Coding RNAs.
LF362138 - JP 2014500723-A/169641: Polycomb-Associated Non-Coding RNAs.
LF362141 - JP 2014500723-A/169644: Polycomb-Associated Non-Coding RNAs.
LF362145 - JP 2014500723-A/169648: Polycomb-Associated Non-Coding RNAs.
LF362146 - JP 2014500723-A/169649: Polycomb-Associated Non-Coding RNAs.
LF362147 - JP 2014500723-A/169650: Polycomb-Associated Non-Coding RNAs.
CU674214 - Synthetic construct Homo sapiens gateway clone IMAGE:100017754 5' read PDIA4 mRNA.
LF362149 - JP 2014500723-A/169652: Polycomb-Associated Non-Coding RNAs.
LF362150 - JP 2014500723-A/169653: Polycomb-Associated Non-Coding RNAs.
LF362153 - JP 2014500723-A/169656: Polycomb-Associated Non-Coding RNAs.
LF362154 - JP 2014500723-A/169657: Polycomb-Associated Non-Coding RNAs.
LF362155 - JP 2014500723-A/169658: Polycomb-Associated Non-Coding RNAs.
LF362156 - JP 2014500723-A/169659: Polycomb-Associated Non-Coding RNAs.
LF362158 - JP 2014500723-A/169661: Polycomb-Associated Non-Coding RNAs.
LF362161 - JP 2014500723-A/169664: Polycomb-Associated Non-Coding RNAs.
MA619681 - JP 2018138019-A/191607: Polycomb-Associated Non-Coding RNAs.
MA597708 - JP 2018138019-A/169634: Polycomb-Associated Non-Coding RNAs.
MA597709 - JP 2018138019-A/169635: Polycomb-Associated Non-Coding RNAs.
MA597712 - JP 2018138019-A/169638: Polycomb-Associated Non-Coding RNAs.
MA597713 - JP 2018138019-A/169639: Polycomb-Associated Non-Coding RNAs.
MA597715 - JP 2018138019-A/169641: Polycomb-Associated Non-Coding RNAs.
MA597718 - JP 2018138019-A/169644: Polycomb-Associated Non-Coding RNAs.
MA597722 - JP 2018138019-A/169648: Polycomb-Associated Non-Coding RNAs.
MA597723 - JP 2018138019-A/169649: Polycomb-Associated Non-Coding RNAs.
MA597724 - JP 2018138019-A/169650: Polycomb-Associated Non-Coding RNAs.
MA597726 - JP 2018138019-A/169652: Polycomb-Associated Non-Coding RNAs.
MA597727 - JP 2018138019-A/169653: Polycomb-Associated Non-Coding RNAs.
MA597730 - JP 2018138019-A/169656: Polycomb-Associated Non-Coding RNAs.
MA597731 - JP 2018138019-A/169657: Polycomb-Associated Non-Coding RNAs.
MA597732 - JP 2018138019-A/169658: Polycomb-Associated Non-Coding RNAs.
MA597733 - JP 2018138019-A/169659: Polycomb-Associated Non-Coding RNAs.
MA597735 - JP 2018138019-A/169661: Polycomb-Associated Non-Coding RNAs.
MA597738 - JP 2018138019-A/169664: Polycomb-Associated Non-Coding RNAs.

-  Other Names for This Gene
  Alternate Gene Symbols: A8K4K6, ERP70, ERP72, NR_163909, P13667, PDIA4_HUMAN, Q549T6, uc328uek.1, uc328uek.2
UCSC ID: ENST00000652332.1_11
RefSeq Accession: NM_004911.5
Protein: P13667 (aka PDIA4_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.