Human Gene PGA3 (ENST00000325558.11_4) from GENCODE V47lift37

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Description: pepsinogen A3 (from RefSeq NM_001079807.4)
Gencode Transcript: ENST00000325558.11_4
Gencode Gene: ENSG00000229859.10_8
Transcript (Including UTRs)
Position: hg19 chr11:60,970,987-60,980,570 Size: 9,584 Total Exon Count: 9 Strand: +
Coding Region
Position: hg19 chr11:60,971,037-60,980,174 Size: 9,138 Coding Exon Count: 9

Page Index	Sequence and Links	UniProtKB Comments	Primers	MalaCards	CTD
Gene Alleles	RNA-Seq Expression	Microarray Expression	RNA Structure	Protein Structure	Other Species
GO Annotations	mRNA Descriptions	Pathways	Other Names	Model Information	Methods

Data last updated at UCSC: 2024-08-22 23:36:26

Sequence and Links to Tools and Databases

Genomic Sequence (chr11:60,970,987-60,980,570)			mRNA (may differ from genome)		Protein (388 aa)
Gene Sorter	Genome Browser	Other Species FASTA	VisiGene	Gene interactions	Table Schema
AlphaFold	BioGPS	Ensembl	Entrez Gene	ExonPrimer	GeneCards
HGNC	Malacards	MGI	OMIM	PubMed	Reactome
UniProtKB	Wikipedia	BioGrid CRISPR DB

Comments and Description Text from UniProtKB


	ID: PEPA3_HUMAN DESCRIPTION: RecName: Full=Pepsin A-3; EC=3.4.23.1; AltName: Full=Pepsinogen-3; Flags: Precursor; FUNCTION: Shows particularly broad specificity; although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent. CATALYTIC ACTIVITY: Preferential cleavage: hydrophobic, preferably aromatic, residues in P1 and P1' positions. Cleaves 1-Phe-\|-Val-2, 4-Gln-\|-His-5, 13-Glu-\|-Ala-14, 14-Ala-\|-Leu-15, 15-Leu-\|-Tyr-16, 16-Tyr-\|-Leu-17, 23-Gly-\|-Phe-24, 24-Phe-\|-Phe-25 and 25-Phe-\|- Tyr-26 bonds in the B chain of insulin. SUBCELLULAR LOCATION: Secreted. SIMILARITY: Belongs to the peptidase A1 family.

Primer design for this transcript

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3

MalaCards Disease Associations


	MalaCards Gene Search: PGA3 Diseases sorted by gene-association score: atrophic gastritis (7), gastritis (5), amyotrophic lateral sclerosis 1 (1)

Comparative Toxicogenomics Database (CTD)


	The following chemicals interact with this gene D007854 Lead

Common Gene Haplotype Alleles


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RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)


	Highest median expression: 2004.32 RPKM in Stomach Total median expression: 2008.65 RPKM View in GTEx track of Genome Browser View at GTEx portal View GTEx Body Map

Microarray Expression Data


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mRNA Secondary Structure of 3' and 5' UTRs

Region	Fold Energy	Bases	Energy/Base	Display As
5' UTR	-15.70	50	-0.314	Picture	PostScript	Text
3' UTR	-113.80	396	-0.287	Picture	PostScript	Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

Protein Domain and Structure Information

InterPro Domains: Graphical view of domain structure
IPR001461 - Peptidase_A1
IPR021109 - Peptidase_aspartic
IPR001969 - Peptidase_aspartic_AS
IPR009007 - Peptidase_aspartic_catalytic
IPR012848 - Propep_A1

Pfam Domains:
PF00026 - Eukaryotic aspartyl protease
PF07966 - A1 Propeptide
PF14543 - Xylanase inhibitor N-terminal

SCOP Domains:
50630 - Acid proteases

ModBase Predicted Comparative 3D Structure on P0DJD8


Front	Top	Side

The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

Orthologous Genes in Other Species

Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.

Mouse	Rat	Zebrafish	D. melanogaster	C. elegans	S. cerevisiae
No ortholog	No ortholog	No ortholog	No ortholog	No ortholog	No ortholog
Gene Details
Gene Sorter

Gene Ontology (GO) Annotations with Structured Vocabulary


	Molecular Function: GO:0004190 aspartic-type endopeptidase activity GO:0008233 peptidase activity GO:0016787 hydrolase activity Biological Process: GO:0006508 proteolysis GO:0006914 autophagy GO:0007586 digestion GO:0030163 protein catabolic process GO:0044267 cellular protein metabolic process Cellular Component: GO:0005576 extracellular region GO:0070062 extracellular exosome GO:0097486 multivesicular body lumen

Descriptions from all associated GenBank mRNAs


	JD200786 - Sequence 181810 from Patent EP1572962. JD465411 - Sequence 446435 from Patent EP1572962. AK312941 - Homo sapiens cDNA, FLJ93393, highly similar to Homo sapiens pepsinogen 5, group I (pepsinogen A) (PGA5), mRNA. AK301293 - Homo sapiens cDNA FLJ60007 complete cds, highly similar to Pepsin A precursor (EC 3.4.23.1). AK301305 - Homo sapiens cDNA FLJ58952 complete cds, highly similar to Pepsin A precursor (EC 3.4.23.1). AK301286 - Homo sapiens cDNA FLJ60006 complete cds, highly similar to Pepsin A precursor (EC 3.4.23.1). AK291864 - Homo sapiens cDNA FLJ77962 complete cds. AK225669 - Homo sapiens mRNA for Pepsin A precursor variant, clone: STM08122. AK225674 - Homo sapiens mRNA for Pepsin A precursor variant, clone: STM08165. AK125628 - Homo sapiens cDNA FLJ43640 fis, clone STOMA2000168, highly similar to PEPSIN A PRECURSOR (EC 3.4.23.1). AK225679 - Homo sapiens mRNA for Pepsin A precursor variant, clone: STM08066. JD402451 - Sequence 383475 from Patent EP1572962. BC150659 - Homo sapiens pepsinogen 4, group I (pepsinogen A), mRNA (cDNA clone MGC:183570 IMAGE:9057030), complete cds. BC171808 - Homo sapiens pepsinogen 4, group I (pepsinogen A), mRNA (cDNA clone MGC:198523 IMAGE:9054462), complete cds. BC171814 - Homo sapiens pepsinogen 4, group I (pepsinogen A), mRNA (cDNA clone MGC:198529 IMAGE:9054468), complete cds. BC171815 - Homo sapiens pepsinogen 3, group I (pepsinogen A), mRNA (cDNA clone MGC:198530 IMAGE:9054469), complete cds. BC171910 - Homo sapiens pepsinogen 4, group I (pepsinogen A), mRNA (cDNA clone MGC:198625 IMAGE:9054564), complete cds. BC171920 - Homo sapiens pepsinogen 4, group I (pepsinogen A), mRNA (cDNA clone MGC:198635 IMAGE:9054574), complete cds. KX856064 - Homo sapiens pregnancy-associated glycoprotein mRNA, complete cds. BC152844 - Synthetic construct Homo sapiens clone IMAGE:100016085, MGC:184199 pepsinogen 4, group I (pepsinogen A) (PGA4) mRNA, encodes complete protein. BC160184 - Synthetic construct Homo sapiens clone IMAGE:100064216, MGC:193299 pepsinogen 3, group I (pepsinogen A) (PGA3) mRNA, encodes complete protein. AB528778 - Synthetic construct DNA, clone: pF1KE0243, Homo sapiens PGA4 gene for pepsinogen 4, group I, without stop codon, in Flexi system. JD194045 - Sequence 175069 from Patent EP1572962. JD450690 - Sequence 431714 from Patent EP1572962. JD033282 - Sequence 14306 from Patent EP1572962. AL832946 - Homo sapiens mRNA; cDNA DKFZp666J2410 (from clone DKFZp666J2410). JD023172 - Sequence 4196 from Patent EP1572962. JD035985 - Sequence 17009 from Patent EP1572962. JD020735 - Sequence 1759 from Patent EP1572962. JD033527 - Sequence 14551 from Patent EP1572962. JD019271 - Sequence 295 from Patent EP1572962. JD022843 - Sequence 3867 from Patent EP1572962. JD035979 - Sequence 17003 from Patent EP1572962. JD308988 - Sequence 290012 from Patent EP1572962. JD182965 - Sequence 163989 from Patent EP1572962. JD411014 - Sequence 392038 from Patent EP1572962.

Biochemical and Signaling Pathways


	Reactome (by CSHL, EBI, and GO) Protein P0DJD8 (Reactome details) participates in the following event(s): R-HSA-5684864 NAPSA, CTSH, PGA3-5 cleave pro-SFTPB R-HSA-5685902 NAPSA, CTSH, PGA3-5 cleave pro-SFTPC R-HSA-5683826 Surfactant metabolism R-HSA-392499 Metabolism of proteins

Other Names for This Gene


	Alternate Gene Symbols: A8K749, B2R7D6, B7ZW75, ENST00000325558.1, ENST00000325558.10, ENST00000325558.2, ENST00000325558.3, ENST00000325558.4, ENST00000325558.5, ENST00000325558.6, ENST00000325558.7, ENST00000325558.8, ENST00000325558.9, NM_001079807, P00790, P0DJD8, PEPA3_HUMAN, Q7M4R0, Q8N1E3, uc317rwv.1, uc317rwv.2 UCSC ID: ENST00000325558.11_4 RefSeq Accession: NM_001079807.4 Protein: P0DJD8 (aka PEPA3_HUMAN)

Gene Model Information


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Methods, Credits, and Use Restrictions


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