Human Gene PHOSPHO2 (ENST00000359744.8_4) from GENCODE V47lift37
  Description: phosphatase, orphan 2, transcript variant 3 (from RefSeq NM_001199285.2)
Gencode Transcript: ENST00000359744.8_4
Gencode Gene: ENSG00000144362.12_9
Transcript (Including UTRs)
   Position: hg19 chr2:170,550,979-170,558,218 Size: 7,240 Total Exon Count: 4 Strand: +
Coding Region
   Position: hg19 chr2:170,557,482-170,558,207 Size: 726 Coding Exon Count: 1 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
Other NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr2:170,550,979-170,558,218)mRNA (may differ from genome)Protein (241 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMGIPubMedUniProtKBBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: PHOP2_HUMAN
DESCRIPTION: RecName: Full=Pyridoxal phosphate phosphatase PHOSPHO2; EC=3.1.3.74;
FUNCTION: Phosphatase that has high activity toward pyridoxal 5'- phosphate (PLP). Also active at much lower level toward pyrophosphate, phosphoethanolamine (PEA), phosphocholine (PCho), phospho-l-tyrosine, fructose-6-phosphate, p-nitrophenyl phosphate, and h-glycerophosphate.
CATALYTIC ACTIVITY: Pyridoxal 5'-phosphate + H(2)O = pyridoxal + phosphate.
COFACTOR: Magnesium (By similarity).
BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=45.5 uM for pyridoxal 5'-phosphate; Vmax=633 nmol/min/mg enzyme with pyridoxal 5'-phosphate as substrate;
SIMILARITY: Belongs to the HAD-like hydrolase superfamily. PHOSPHO family.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 14.21 RPKM in Testis
Total median expression: 117.20 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -121.50384-0.316 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR023214 - HAD-like_dom
IPR006383 - HAD-SF_hydro_IB_PSP-like
IPR016965 - Pase_PHOSPHO-typ
IPR006384 - PyrdxlP_Pase-rel

Pfam Domains:
PF06888 - Putative Phosphatase
PF12710 - haloacid dehalogenase-like hydrolase

SCOP Domains:
56784 - HAD-like

ModBase Predicted Comparative 3D Structure on Q8TCD6
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details Gene Details  
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0005515 protein binding
GO:0016787 hydrolase activity
GO:0016791 phosphatase activity
GO:0033883 pyridoxal phosphatase activity
GO:0046872 metal ion binding

Biological Process:
GO:0016311 dephosphorylation


-  Descriptions from all associated GenBank mRNAs
  AK314915 - Homo sapiens cDNA, FLJ95823.
LF383696 - JP 2014500723-A/191199: Polycomb-Associated Non-Coding RNAs.
BC106013 - Homo sapiens phosphatase, orphan 2, mRNA (cDNA clone MGC:111048 IMAGE:4387304), complete cds.
AK090653 - Homo sapiens cDNA FLJ33334 fis, clone BRACE2000733, weakly similar to RING CANAL PROTEIN.
AX746559 - Sequence 84 from Patent EP1308459.
BC022324 - Homo sapiens phosphatase, orphan 2, mRNA (cDNA clone MGC:22679 IMAGE:4701753), complete cds.
CU692558 - Synthetic construct Homo sapiens gateway clone IMAGE:100022114 5' read PHOSPHO2 mRNA.
HQ447744 - Synthetic construct Homo sapiens clone IMAGE:100071075; CCSB013036_01 phosphatase, orphan 2 (PHOSPHO2) gene, encodes complete protein.
KJ896302 - Synthetic construct Homo sapiens clone ccsbBroadEn_05696 PHOSPHO2 gene, encodes complete protein.
LF347425 - JP 2014500723-A/154928: Polycomb-Associated Non-Coding RNAs.
MA619273 - JP 2018138019-A/191199: Polycomb-Associated Non-Coding RNAs.
MA583002 - JP 2018138019-A/154928: Polycomb-Associated Non-Coding RNAs.

-  Other Names for This Gene
  Alternate Gene Symbols: B2RC30, D3DPC7, ENST00000359744.1, ENST00000359744.2, ENST00000359744.3, ENST00000359744.4, ENST00000359744.5, ENST00000359744.6, ENST00000359744.7, NM_001199285, PHOP2_HUMAN, Q8TCD6, uc318bew.1, uc318bew.2
UCSC ID: ENST00000359744.8_4
RefSeq Accession: NM_001008489.4
Protein: Q8TCD6 (aka PHOP2_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.