Human Gene PIGU (ENST00000217446.8_9) from GENCODE V47lift37
  Description: phosphatidylinositol glycan anchor biosynthesis class U (from RefSeq NM_080476.5)
Gencode Transcript: ENST00000217446.8_9
Gencode Gene: ENSG00000101464.11_11
Transcript (Including UTRs)
   Position: hg19 chr20:33,148,346-33,264,896 Size: 116,551 Total Exon Count: 12 Strand: -
Coding Region
   Position: hg19 chr20:33,148,670-33,264,889 Size: 116,220 Coding Exon Count: 12 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr20:33,148,346-33,264,896)mRNA (may differ from genome)Protein (435 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGeneCardsHGNC
MGIOMIMPubMedReactomeUniProtKBWikipedia
BioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: PIGU_HUMAN
DESCRIPTION: RecName: Full=Phosphatidylinositol glycan anchor biosynthesis class U protein; AltName: Full=Cell division cycle protein 91-like 1; Short=Protein CDC91-like 1; AltName: Full=GPI transamidase component PIG-U;
FUNCTION: Component of the GPI transamidase complex. May be involved in the recognition of either the GPI attachment signal or the lipid portion of GPI.
PATHWAY: Glycolipid biosynthesis; glycosylphosphatidylinositol- anchor biosynthesis.
SUBUNIT: Forms a complex with PIGK/GPI8, PIGS, PIGT and GPAA1/GAA1.
SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass membrane protein (Potential).
SIMILARITY: Belongs to the PIGU family.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 15.44 RPKM in Testis
Total median expression: 382.30 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
3' UTR -125.00324-0.386 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR009600 - PIG-U

Pfam Domains:
PF06728 - GPI transamidase subunit PIG-U

ModBase Predicted Comparative 3D Structure on Q9H490
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologGenome BrowserGenome Browser
Gene DetailsGene Details Gene DetailsGene DetailsGene Details
Gene SorterGene Sorter Gene SorterGene SorterGene Sorter
 RGDEnsembl WormBaseSGD
    Protein SequenceProtein Sequence
    AlignmentAlignment

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0003923 GPI-anchor transamidase activity
GO:0034235 GPI anchor binding

Biological Process:
GO:0006506 GPI anchor biosynthetic process
GO:0016255 attachment of GPI anchor to protein
GO:0046425 regulation of JAK-STAT cascade

Cellular Component:
GO:0005783 endoplasmic reticulum
GO:0005789 endoplasmic reticulum membrane
GO:0005886 plasma membrane
GO:0016020 membrane
GO:0016021 integral component of membrane
GO:0030176 integral component of endoplasmic reticulum membrane
GO:0042765 GPI-anchor transamidase complex


-  Descriptions from all associated GenBank mRNAs
  BC030512 - Homo sapiens phosphatidylinositol glycan anchor biosynthesis, class U, mRNA (cDNA clone MGC:40420 IMAGE:4942673), complete cds.
AY422169 - Homo sapiens transamidase complex subunit PIG-U (PIGU) mRNA, complete cds.
AK075507 - Homo sapiens cDNA PSEC0205 fis, clone HEMBA1002715, highly similar to Cell division cycle protein 91-like 1.
AY358816 - Homo sapiens clone DNA119544 CDC91L1 (UNQ3055) mRNA, complete cds.
AB086842 - Homo sapiens PIGU mRNA for PIG-U, complete cds.
AK301900 - Homo sapiens cDNA FLJ58335 complete cds, highly similar to GPI transamidase component PIG-U.
AY339061 - Homo sapiens cell division cycle 91-like 1 (CDC91L1) mRNA, complete cds; alternatively spliced.
EU831773 - Synthetic construct Homo sapiens clone HAIB:100066802; DKFZo008C0521 phosphatidylinositol glycan anchor biosynthesis, class U protein (PIGU) gene, encodes complete protein.
EU831851 - Synthetic construct Homo sapiens clone HAIB:100066880; DKFZo004C0522 phosphatidylinositol glycan anchor biosynthesis, class U protein (PIGU) gene, encodes complete protein.
AK095044 - Homo sapiens cDNA FLJ37725 fis, clone BRHIP2019641.
AK095356 - Homo sapiens cDNA FLJ38037 fis, clone CTONG2013803.
JD438198 - Sequence 419222 from Patent EP1572962.
JD390486 - Sequence 371510 from Patent EP1572962.
JD382183 - Sequence 363207 from Patent EP1572962.
JD470885 - Sequence 451909 from Patent EP1572962.
JD278632 - Sequence 259656 from Patent EP1572962.
JD480766 - Sequence 461790 from Patent EP1572962.
JD218253 - Sequence 199277 from Patent EP1572962.
JD231023 - Sequence 212047 from Patent EP1572962.
JD465048 - Sequence 446072 from Patent EP1572962.
HM245394 - Homo sapiens ALG5-PIGU breakpoint junction mRNA sequence.
AK308627 - Homo sapiens cDNA, FLJ98668.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q9H490 (Reactome details) participates in the following event(s):

R-HSA-162836 uPAR precursor + acyl-GPI -> uPAR-acyl-GPI + uPAR propeptide
R-HSA-162791 Attachment of GPI anchor to uPAR
R-HSA-163125 Post-translational modification: synthesis of GPI-anchored proteins
R-HSA-597592 Post-translational protein modification
R-HSA-392499 Metabolism of proteins

-  Other Names for This Gene
  Alternate Gene Symbols: CDC91L1, ENST00000217446.1, ENST00000217446.2, ENST00000217446.3, ENST00000217446.4, ENST00000217446.5, ENST00000217446.6, ENST00000217446.7, NM_080476, PIGU_HUMAN, PSEC0205, Q7Z489, Q8N2F2, Q9H490, uc317ctz.1, uc317ctz.2, UNQ3055/PRO9875
UCSC ID: ENST00000217446.8_9
RefSeq Accession: NM_080476.5
Protein: Q9H490 (aka PIGU_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.