Human Gene PIK3R3 (ENST00000262741.10_8) from GENCODE V47lift37
  Description: phosphoinositide-3-kinase regulatory subunit 3, transcript variant 14 (from RefSeq NR_137329.2)
Gencode Transcript: ENST00000262741.10_8
Gencode Gene: ENSG00000117461.15_13
Transcript (Including UTRs)
   Position: hg19 chr1:46,505,812-46,598,301 Size: 92,490 Total Exon Count: 10 Strand: -
Coding Region
   Position: hg19 chr1:46,509,345-46,597,624 Size: 88,280 Coding Exon Count: 10 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
Gene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther Species
GO AnnotationsmRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr1:46,505,812-46,598,301)mRNA (may differ from genome)Protein (461 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
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HGNCMalacardsMGIOMIMPubMedReactome
UniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: P55G_HUMAN
DESCRIPTION: RecName: Full=Phosphatidylinositol 3-kinase regulatory subunit gamma; Short=PI3-kinase regulatory subunit gamma; Short=PI3K regulatory subunit gamma; Short=PtdIns-3-kinase regulatory subunit gamma; AltName: Full=Phosphatidylinositol 3-kinase 55 kDa regulatory subunit gamma; Short=PI3-kinase subunit p55-gamma; Short=PtdIns-3-kinase regulatory subunit p55-gamma; AltName: Full=p55PIK;
FUNCTION: Binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain and regulates their kinase activity. During insulin stimulation, it also binds to IRS-1.
SUBUNIT: Heterodimer of a regulatory subunit PIK3R3 and a p110 catalytic subunit (PIK3CA, PIK3CB or PIK3CD). Interacts with AXL.
TISSUE SPECIFICITY: Highest levels in brain and testis. Lower levels in adipose tissue, kidney, heart, lung and skeletal muscle.
SIMILARITY: Belongs to the PI3K p85 subunit family.
SIMILARITY: Contains 2 SH2 domains.

-  Primer design for this transcript
 

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Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: PIK3R3
Diseases sorted by gene-association score: asbestos-related lung carcinoma (17), intravenous leiomyomatosis (7)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 27.15 RPKM in Brain - Cerebellar Hemisphere
Total median expression: 333.24 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -248.80677-0.368 Picture PostScript Text
3' UTR -1171.903533-0.332 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR001720 - PI3kinase_P85
IPR000980 - SH2

Pfam Domains:
PF00017 - SH2 domain
PF16454 - Phosphatidylinositol 3-kinase regulatory subunit P85 inter-SH2 domain

SCOP Domains:
49785 - Galactose-binding domain-like
55550 - SH2 domain

ModBase Predicted Comparative 3D Structure on Q92569
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0001784 phosphotyrosine binding
GO:0005515 protein binding
GO:0016303 1-phosphatidylinositol-3-kinase activity
GO:0035014 phosphatidylinositol 3-kinase regulator activity
GO:0046935 1-phosphatidylinositol-3-kinase regulator activity

Biological Process:
GO:0008286 insulin receptor signaling pathway
GO:0036092 phosphatidylinositol-3-phosphate biosynthetic process
GO:0043551 regulation of phosphatidylinositol 3-kinase activity
GO:2001275 positive regulation of glucose import in response to insulin stimulus

Cellular Component:
GO:0005829 cytosol
GO:0005942 phosphatidylinositol 3-kinase complex


-  Descriptions from all associated GenBank mRNAs
  CR749649 - Homo sapiens mRNA; cDNA DKFZp686P05226 (from clone DKFZp686P05226).
BC021622 - Homo sapiens phosphoinositide-3-kinase, regulatory subunit 3 (gamma), mRNA (cDNA clone MGC:9142 IMAGE:3910221), complete cds.
BX537388 - Homo sapiens mRNA; cDNA DKFZp686F1293 (from clone DKFZp686F1293).
D88532 - Homo sapiens mRNA for p55pik, complete cds.
BC094768 - Homo sapiens cDNA clone IMAGE:4373927, containing frame-shift errors.
AK302049 - Homo sapiens cDNA FLJ60942 complete cds, highly similar to Phosphatidylinositol 3-kinase regulatory subunit gamma.
AF028785 - Homo sapiens phosphatidylinositol 3-kinase p55 gamma regulatory subunit mRNA, complete cds.
AK313726 - Homo sapiens cDNA, FLJ94325, Homo sapiens phosphoinositide-3-kinase, regulatory subunit,polypeptide 3 (p55, gamma) (PIK3R3), mRNA.
DQ891789 - Synthetic construct clone IMAGE:100004419; FLH179928.01X; RZPDo839E01132D phosphoinositide-3-kinase, regulatory subunit 3 (p55, gamma) (PIK3R3) gene, encodes complete protein.
KJ897855 - Synthetic construct Homo sapiens clone ccsbBroadEn_07249 PIK3R3 gene, encodes complete protein.
KJ905354 - Synthetic construct Homo sapiens clone ccsbBroadEn_14899 PIK3R3 gene, encodes complete protein.
DQ894971 - Synthetic construct Homo sapiens clone IMAGE:100009431; FLH179924.01L; RZPDo839E01131D phosphoinositide-3-kinase, regulatory subunit 3 (p55, gamma) (PIK3R3) gene, encodes complete protein.
EU832448 - Synthetic construct Homo sapiens clone HAIB:100067477; DKFZo008A0529 phosphoinositide-3-kinase, regulatory subunit 3 (gamma) protein (PIK3R3) gene, encodes complete protein.
EU832531 - Synthetic construct Homo sapiens clone HAIB:100067560; DKFZo004A0530 phosphoinositide-3-kinase, regulatory subunit 3 (gamma) protein (PIK3R3) gene, encodes complete protein.
AB384848 - Synthetic construct DNA, clone: pF1KB3734, Homo sapiens PIK3R3 gene for phosphoinositide-3-kinase, regulatory subunit 3, complete cds, without stop codon, in Flexi system.
BC005381 - Homo sapiens phosphoinositide-3-kinase, regulatory subunit 3 (gamma), mRNA (cDNA clone IMAGE:3935147), partial cds.
U90907 - Human clone 23907 mRNA sequence.
JD132238 - Sequence 113262 from Patent EP1572962.
DQ570412 - Homo sapiens piRNA piR-30524, complete sequence.
JD198981 - Sequence 180005 from Patent EP1572962.
JD099929 - Sequence 80953 from Patent EP1572962.
DQ587351 - Homo sapiens piRNA piR-54463, complete sequence.
DQ583875 - Homo sapiens piRNA piR-50987, complete sequence.
JD244701 - Sequence 225725 from Patent EP1572962.
JD475629 - Sequence 456653 from Patent EP1572962.
JD518235 - Sequence 499259 from Patent EP1572962.
JD299532 - Sequence 280556 from Patent EP1572962.
DQ571787 - Homo sapiens piRNA piR-31899, complete sequence.
JD054102 - Sequence 35126 from Patent EP1572962.
JD545734 - Sequence 526758 from Patent EP1572962.
JD300938 - Sequence 281962 from Patent EP1572962.
JD555152 - Sequence 536176 from Patent EP1572962.
JD297735 - Sequence 278759 from Patent EP1572962.
JD235232 - Sequence 216256 from Patent EP1572962.
AK123886 - Homo sapiens cDNA FLJ41892 fis, clone OCBBF2025115, highly similar to Homo sapiens mRNA for p55pik.
JD235154 - Sequence 216178 from Patent EP1572962.
JD295596 - Sequence 276620 from Patent EP1572962.
JD503244 - Sequence 484268 from Patent EP1572962.
JD508924 - Sequence 489948 from Patent EP1572962.
JD083092 - Sequence 64116 from Patent EP1572962.
JD342650 - Sequence 323674 from Patent EP1572962.
AK304713 - Homo sapiens cDNA FLJ55275 complete cds, highly similar to Phosphatidylinositol 3-kinase regulatory subunit gamma.
JD559780 - Sequence 540804 from Patent EP1572962.
JD281213 - Sequence 262237 from Patent EP1572962.
JD174046 - Sequence 155070 from Patent EP1572962.
JD356182 - Sequence 337206 from Patent EP1572962.
JD548820 - Sequence 529844 from Patent EP1572962.
JD060293 - Sequence 41317 from Patent EP1572962.
JD298155 - Sequence 279179 from Patent EP1572962.
JD369273 - Sequence 350297 from Patent EP1572962.
JD292671 - Sequence 273695 from Patent EP1572962.
Y13809 - Homo sapiens partial mRNA for putative phosphatidylinositol 3-kinase regulatory gamma subunit, colon cancer patient, clone PM103.
AK128500 - Homo sapiens cDNA FLJ46650 fis, clone TRACH3004596.
JD402575 - Sequence 383599 from Patent EP1572962.
JD474386 - Sequence 455410 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  BioCyc Knowledge Library
PWY-6352 - 3-phosphoinositide biosynthesis
PWY-6371 - superpathway of inositol phosphate compounds

Reactome (by CSHL, EBI, and GO)

Protein Q92569 (Reactome details) participates in the following event(s):

R-HSA-1295516 IL7:p-Y449-IL7R:JAK1:IL2RG:JAK3 binds PI3K regulatory subunits
R-HSA-114542 Rac1 activation of PI3K
R-HSA-205262 Direct recruitment of PI3K to p-KIT
R-HSA-388830 PI3K binds ICOS
R-HSA-388832 PI3K binds CD28
R-HSA-416358 G alpha (q) inhibits PI3K alpha
R-HSA-1562641 Indirect recruitment of PI3K to KIT via p(Y)-GAB2
R-HSA-1676109 PI4P is phosphorylated to PI(3,4)P2 by PI3K3C[2] at the plasma membrane
R-HSA-1676048 PI(4,5)P2 is phosphorylated to PI(3,4,5)P3 by PIK3C[1] at the plasma membrane
R-HSA-508247 Gab2 binds the p85 subunit of Class 1A PI3 kinases
R-HSA-879917 CBL, GRB2, FYN and PI3K p85 subunit are constitutively associated
R-HSA-914182 14-3-3 zeta binding allows recruitment of PI3K
R-HSA-389158 CD28 bound PI3K phosphorylates PIP2 to PIP3
R-HSA-437162 PI3K alpha, beta, gamma convert PIP2 to PIP3
R-HSA-912629 CBL is tyrosine phosphorylated
R-HSA-2400009 PI3K inhibitors block PI3K catalytic activity
R-HSA-2316434 PI3K phosphorylates PIP2 to PIP3
R-HSA-1433514 Synthesis of PIP3 from PIP2 by PI3K
R-HSA-1266695 Interleukin-7 signaling
R-HSA-416482 G alpha (12/13) signalling events
R-HSA-1433557 Signaling by SCF-KIT
R-HSA-388841 Costimulation by the CD28 family
R-HSA-389357 CD28 dependent PI3K/Akt signaling
R-HSA-416476 G alpha (q) signalling events
R-HSA-114604 GPVI-mediated activation cascade
R-HSA-449147 Signaling by Interleukins
R-HSA-1660499 Synthesis of PIPs at the plasma membrane
R-HSA-912526 Interleukin receptor SHC signaling
R-HSA-912631 Regulation of signaling by CBL
R-HSA-512988 Interleukin-3, 5 and GM-CSF signaling
R-HSA-8853659 RET signaling
R-HSA-388396 GPCR downstream signalling
R-HSA-9006934 Signaling by Receptor Tyrosine Kinases
R-HSA-1280218 Adaptive Immune System
R-HSA-389356 CD28 co-stimulation
R-HSA-76002 Platelet activation, signaling and aggregation
R-HSA-1280215 Cytokine Signaling in Immune system
R-HSA-1483255 PI Metabolism
R-HSA-451927 Interleukin-2 family signaling
R-HSA-422475 Axon guidance
R-HSA-372790 Signaling by GPCR
R-HSA-162582 Signal Transduction
R-HSA-168256 Immune System
R-HSA-109582 Hemostasis
R-HSA-1483257 Phospholipid metabolism
R-HSA-2219530 Constitutive Signaling by Aberrant PI3K in Cancer
R-HSA-1257604 PIP3 activates AKT signaling
R-HSA-6811558 PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling
R-HSA-1266738 Developmental Biology
R-HSA-556833 Metabolism of lipids
R-HSA-2219528 PI3K/AKT Signaling in Cancer
R-HSA-9006925 Intracellular signaling by second messengers
R-HSA-199418 Negative regulation of the PI3K/AKT network
R-HSA-1430728 Metabolism
R-HSA-5663202 Diseases of signal transduction
R-HSA-1643685 Disease

-  Other Names for This Gene
  Alternate Gene Symbols: B2R9C1, D3DQ12, ENST00000262741.1, ENST00000262741.2, ENST00000262741.3, ENST00000262741.4, ENST00000262741.5, ENST00000262741.6, ENST00000262741.7, ENST00000262741.8, ENST00000262741.9, NR_137329, O60482, P55G_HUMAN, Q5T4P1, Q5T4P2, Q92569, uc317hdb.1, uc317hdb.2
UCSC ID: ENST00000262741.10_8
RefSeq Accession: NM_003629.4
Protein: Q92569 (aka P55G_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.