ID:PJA1_HUMAN DESCRIPTION: RecName: Full=E3 ubiquitin-protein ligase Praja-1; Short=Praja1; EC=6.3.2.-; AltName: Full=RING finger protein 70; FUNCTION: Has E2-dependent E3 ubiquitin-protein ligase activity. Ubiquitinates MAGED1 antigen leading to its subsequent degradation by proteasome (By similarity). May be involved in protein sorting. SUBUNIT: Binds ubiquitin-conjugating enzymes (E2s). In vitro, interacts with the ubiquitin-conjugating enzyme, UBE2D2. INTERACTION: Q9Y5V3:MAGED1; NbExp=4; IntAct=EBI-714606, EBI-716006; Q96MG7:NDNL2; NbExp=2; IntAct=EBI-714606, EBI-2557356; TISSUE SPECIFICITY: Expressed in various regions of the brain including the cerebellum, cerebral cortex, medulla, occipital pole, frontal lobe, temporal lobe and putamen. Highest levels in the cerebral cortex. DOMAIN: The RING-type zinc finger domain interacts with an ubiquitin-conjugating enzyme (E2) and facilitates ubiquitination (By similarity). PTM: Substrate for E2-dependent ubiquitination. SIMILARITY: Contains 1 RING-type zinc finger. SEQUENCE CAUTION: Sequence=BAB13928.1; Type=Erroneous initiation;
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on Q8NG27
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
LF211587 - JP 2014500723-A/19090: Polycomb-Associated Non-Coding RNAs. AK294632 - Homo sapiens cDNA FLJ55052 complete cds, highly similar to Ubiquitin protein ligase Praja1 (EC 6.3.2.-). BC048323 - Homo sapiens praja ring finger 1, mRNA (cDNA clone IMAGE:4447276), partial cds. AF262024 - Homo sapiens PJA1 (PJA1) mRNA, complete cds. AF264620 - Homo sapiens PRAJA1BETA (PRAJA1BETA) mRNA, complete cds. BC058327 - Homo sapiens praja ring finger 1, mRNA (cDNA clone IMAGE:6163084). BC039149 - Homo sapiens, Similar to praja1, RING-H2 motif containing, clone IMAGE:4752521, mRNA. BC075803 - Homo sapiens praja ring finger 1, mRNA (cDNA clone MGC:87793 IMAGE:3913310), complete cds. AK021892 - Homo sapiens cDNA FLJ11830 fis, clone HEMBA1006559, moderately similar to Mus musculus PRAJA1 (Praja1) mRNA. AK096611 - Homo sapiens cDNA FLJ39292 fis, clone OCBBF2012553, highly similar to Ubiquitin protein ligase Praja1 (EC 6.3.2.-). AK290543 - Homo sapiens cDNA FLJ78308 complete cds, highly similar to Homo sapiens praja 1 (PJA1), mRNA. AK226171 - Homo sapiens mRNA for praja 1 isoform a variant, clone: bm01524. AK293266 - Homo sapiens cDNA FLJ53433 complete cds, highly similar to Ubiquitin protein ligase Praja1 (EC 6.3.2.-). BC105051 - Homo sapiens praja ring finger 1, mRNA (cDNA clone MGC:132711 IMAGE:8144054), complete cds. BC105053 - Homo sapiens praja ring finger 1, mRNA (cDNA clone MGC:132713 IMAGE:8144056), complete cds. KJ894542 - Synthetic construct Homo sapiens clone ccsbBroadEn_03936 PJA1 gene, encodes complete protein. EU446995 - Synthetic construct Homo sapiens clone IMAGE:100070113; IMAGE:100012204; FLH257539.01L praja 1 (PJA1) gene, encodes complete protein. AB527200 - Synthetic construct DNA, clone: pF1KB5190, Homo sapiens PJA1 gene for praja ring finger 1, without stop codon, in Flexi system. LF381619 - JP 2014500723-A/189122: Polycomb-Associated Non-Coding RNAs. LF381620 - JP 2014500723-A/189123: Polycomb-Associated Non-Coding RNAs. LF381621 - JP 2014500723-A/189124: Polycomb-Associated Non-Coding RNAs. BC113035 - Homo sapiens praja ring finger 1, mRNA (cDNA clone IMAGE:40021896), partial cds. CU687292 - Synthetic construct Homo sapiens gateway clone IMAGE:100022841 5' read PJA1 mRNA. LF381622 - JP 2014500723-A/189125: Polycomb-Associated Non-Coding RNAs. LF381623 - JP 2014500723-A/189126: Polycomb-Associated Non-Coding RNAs. LF381624 - JP 2014500723-A/189127: Polycomb-Associated Non-Coding RNAs. JD175780 - Sequence 156804 from Patent EP1572962. MA447164 - JP 2018138019-A/19090: Polycomb-Associated Non-Coding RNAs. MA617196 - JP 2018138019-A/189122: Polycomb-Associated Non-Coding RNAs. MA617197 - JP 2018138019-A/189123: Polycomb-Associated Non-Coding RNAs. MA617198 - JP 2018138019-A/189124: Polycomb-Associated Non-Coding RNAs. MA617199 - JP 2018138019-A/189125: Polycomb-Associated Non-Coding RNAs. MA617200 - JP 2018138019-A/189126: Polycomb-Associated Non-Coding RNAs. MA617201 - JP 2018138019-A/189127: Polycomb-Associated Non-Coding RNAs.
Biochemical and Signaling Pathways
Reactome (by CSHL, EBI, and GO)
Protein Q8NG27 (Reactome details) participates in the following event(s):
R-HSA-983157 Interaction of E3 with substrate and E2-Ub complex R-HSA-983147 Release of E3 from polyubiquitinated substrate R-HSA-983140 Transfer of Ub from E2 to substrate and release of E2 R-HSA-983168 Antigen processing: Ubiquitination & Proteasome degradation R-HSA-983169 Class I MHC mediated antigen processing & presentation R-HSA-1280218 Adaptive Immune System R-HSA-168256 Immune System
US Server
Sequence and Links to Tools and Databases 
Common Gene Haplotype Alleles 
