Human Gene PKM (ENST00000335181.10_15) from GENCODE V47lift37
  Description: pyruvate kinase M1/2, transcript variant 1 (from RefSeq NM_002654.6)
Gencode Transcript: ENST00000335181.10_15
Gencode Gene: ENSG00000067225.21_16
Transcript (Including UTRs)
   Position: hg19 chr15:72,491,370-72,523,531 Size: 32,162 Total Exon Count: 11 Strand: -
Coding Region
   Position: hg19 chr15:72,491,991-72,511,438 Size: 19,448 Coding Exon Count: 10 

Page IndexSequence and LinksPrimersMalaCardsCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureOther SpeciesmRNA DescriptionsPathways
Other NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr15:72,491,370-72,523,531)mRNA (may differ from genome)Protein (531 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMalacardsMGIOMIMPubMedUniProtKB
WikipediaBioGrid CRISPR DB

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: PKM
Diseases sorted by gene-association score: pyruvate kinase deficiency (15), acute diarrhea (8), pigmentation disease (7), atrichia with papular lesions (7), linitis plastica (7), gastric diffuse adenocarcinoma (6), kernicterus (6), li-fraumeni syndrome (5), bilirubin metabolic disorder (4), colorectal cancer (1)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 664.82 RPKM in Cells - EBV-transformed lymphocytes
Total median expression: 8863.57 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -25.5088-0.290 Picture PostScript Text
3' UTR -199.90621-0.322 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologGenome Browser
Gene DetailsGene Details   Gene Details
Gene SorterGene Sorter   Gene Sorter
 RGD   SGD
     Protein Sequence
     Alignment

-  Descriptions from all associated GenBank mRNAs
  BC035487 - Homo sapiens pyruvate kinase, muscle, mRNA (cDNA clone IMAGE:4779421), with apparent retained intron.
BC051346 - Homo sapiens cDNA clone IMAGE:6461886, **** WARNING: chimeric clone ****.
BC035198 - Homo sapiens pyruvate kinase, muscle, mRNA (cDNA clone MGC:9369 IMAGE:3859987), complete cds.
M26252 - Human TCB gene encoding cytosolic thyroid hormone-binding protein, complete cds.
EU794631 - Homo sapiens epididymis secretory protein Li 30 (HEL-S-30) mRNA, complete cds.
HZ489613 - JP 2016020328-A/13: ANTICANCER AGENT.
LX088621 - JP 2017075124-A/1: DRUG SENSITIVITY ENHANCING AGENT FOR CANCER CELL, METHOD FOR PREDICTING DRUG SENSITIVITY, AND DRUG SENSITIVITY BIOMARKER.
AF025439 - Homo sapiens Opa-interacting protein OIP3 mRNA, partial cds.
BC019265 - Homo sapiens pyruvate kinase, muscle, mRNA (cDNA clone IMAGE:3503708), partial cds.
BC023328 - Homo sapiens pyruvate kinase, muscle, mRNA (cDNA clone IMAGE:4510296).
BC023592 - Homo sapiens pyruvate kinase, muscle, mRNA (cDNA clone IMAGE:4646848).
BC096823 - Homo sapiens pyruvate kinase, muscle, mRNA (cDNA clone IMAGE:30558692).
BC007640 - Homo sapiens pyruvate kinase, muscle, mRNA (cDNA clone MGC:15908 IMAGE:3533876), complete cds.
BC012811 - Homo sapiens pyruvate kinase, muscle, mRNA (cDNA clone MGC:3932 IMAGE:2958817), complete cds.
BC000481 - Homo sapiens pyruvate kinase, muscle, mRNA (cDNA clone MGC:8698 IMAGE:2964687), complete cds.
BC094767 - Homo sapiens pyruvate kinase, muscle, mRNA (cDNA clone MGC:104654 IMAGE:5296639), complete cds.
AK092369 - Homo sapiens cDNA FLJ35050 fis, clone OCBBF2018167, highly similar to Pyruvate kinase isozyme M1 (EC 2.7.1.40).
AK299413 - Homo sapiens cDNA FLJ53645 complete cds, highly similar to Pyruvate kinase isozyme M1 (EC 2.7.1.40).
BX648597 - Homo sapiens mRNA; cDNA DKFZp686G12209 (from clone DKFZp686G12209).
BC007952 - Homo sapiens pyruvate kinase, muscle, mRNA (cDNA clone MGC:14360 IMAGE:4299213), complete cds.
AK297951 - Homo sapiens cDNA FLJ53368 complete cds, highly similar to Pyruvate kinase isozymes M1/M2 (EC 2.7.1.40).
M23725 - Human M2-type pyruvate kinase mRNA, complete cds.
JD253237 - Sequence 234261 from Patent EP1572962.
JD554754 - Sequence 535778 from Patent EP1572962.
AK222927 - Homo sapiens mRNA for pyruvate kinase 3 isoform 1 variant, clone: HRC08174.
JD120305 - Sequence 101329 from Patent EP1572962.
JD360415 - Sequence 341439 from Patent EP1572962.
JD067018 - Sequence 48042 from Patent EP1572962.
AK300800 - Homo sapiens cDNA FLJ54554 complete cds, highly similar to Pyruvate kinase isozymes M1/M2 (EC 2.7.1.40).
JD186650 - Sequence 167674 from Patent EP1572962.
JD227184 - Sequence 208208 from Patent EP1572962.
JD323231 - Sequence 304255 from Patent EP1572962.
JD385499 - Sequence 366523 from Patent EP1572962.
JD174331 - Sequence 155355 from Patent EP1572962.
JD024294 - Sequence 5318 from Patent EP1572962.
JD030195 - Sequence 11219 from Patent EP1572962.
AK307855 - Homo sapiens cDNA, FLJ97803.
JD026312 - Sequence 7336 from Patent EP1572962.
JD403809 - Sequence 384833 from Patent EP1572962.
JD021887 - Sequence 2911 from Patent EP1572962.
JD029525 - Sequence 10549 from Patent EP1572962.
JD391000 - Sequence 372024 from Patent EP1572962.
JD540550 - Sequence 521574 from Patent EP1572962.
JD540025 - Sequence 521049 from Patent EP1572962.
JD042742 - Sequence 23766 from Patent EP1572962.
JD047097 - Sequence 28121 from Patent EP1572962.
JD326238 - Sequence 307262 from Patent EP1572962.
JD131573 - Sequence 112597 from Patent EP1572962.
JD369818 - Sequence 350842 from Patent EP1572962.
AK294315 - Homo sapiens cDNA FLJ56065 complete cds, highly similar to Pyruvate kinase isozyme M1 (EC 2.7.1.40).
AK309781 - Homo sapiens cDNA, FLJ99822.
JD561983 - Sequence 543007 from Patent EP1572962.
EU832358 - Synthetic construct Homo sapiens clone HAIB:100067387; DKFZo008H1227 pyruvate kinase, muscle protein (PKM2) gene, encodes complete protein.
AK312253 - Homo sapiens cDNA, FLJ92548, highly similar to Homo sapiens pyruvate kinase, muscle (PKM2), mRNA.
KJ891817 - Synthetic construct Homo sapiens clone ccsbBroadEn_01211 PKM2 gene, encodes complete protein.
KJ905271 - Synthetic construct Homo sapiens clone ccsbBroadEn_14768 PKM2 gene, encodes complete protein.
KR710112 - Synthetic construct Homo sapiens clone CCSBHm_00009784 PKM (PKM) mRNA, encodes complete protein.
KR710113 - Synthetic construct Homo sapiens clone CCSBHm_00009785 PKM (PKM) mRNA, encodes complete protein.
EU832443 - Synthetic construct Homo sapiens clone HAIB:100067472; DKFZo004H1228 pyruvate kinase, muscle protein (PKM2) gene, encodes complete protein.
DQ892739 - Synthetic construct clone IMAGE:100005369; FLH189257.01X; RZPDo839D0374D pyruvate kinase, muscle (PKM2) gene, encodes complete protein.
EU176742 - Synthetic construct Homo sapiens clone IMAGE:100011510; FLH189256.01L; RZPDo839G10255D pyruvate kinase, muscle (PKM2) gene, encodes complete protein.
AB528306 - Synthetic construct DNA, clone: pF1KB9947, Homo sapiens PKM2 gene for pyruvate kinase, muscle, without stop codon, in Flexi system.
HZ473969 - WO 2016002844-A/83: ANTI-INVASIVE/ANTI-METASTATIC DRUG FOR PANCREATIC CANCER CELL.
AK308264 - Homo sapiens cDNA, FLJ98212.
HZ489614 - JP 2016020328-A/14: ANTICANCER AGENT.
JD022966 - Sequence 3990 from Patent EP1572962.
JD022355 - Sequence 3379 from Patent EP1572962.
JD027715 - Sequence 8739 from Patent EP1572962.
JD019461 - Sequence 485 from Patent EP1572962.
JD035609 - Sequence 16633 from Patent EP1572962.
Z36786 - H.sapiens (xs130) mRNA, 260bp.
HZ473968 - WO 2016002844-A/82: ANTI-INVASIVE/ANTI-METASTATIC DRUG FOR PANCREATIC CANCER CELL.
JD024738 - Sequence 5762 from Patent EP1572962.
JD033454 - Sequence 14478 from Patent EP1572962.
JD027287 - Sequence 8311 from Patent EP1572962.
JD031777 - Sequence 12801 from Patent EP1572962.
HZ473970 - WO 2016002844-A/84: ANTI-INVASIVE/ANTI-METASTATIC DRUG FOR PANCREATIC CANCER CELL.
Z36831 - H.sapiens (xs25) mRNA, 443bp.
HZ473967 - WO 2016002844-A/81: ANTI-INVASIVE/ANTI-METASTATIC DRUG FOR PANCREATIC CANCER CELL.
JD027499 - Sequence 8523 from Patent EP1572962.
JD031362 - Sequence 12386 from Patent EP1572962.
LF208528 - JP 2014500723-A/16031: Polycomb-Associated Non-Coding RNAs.
LF377353 - JP 2014500723-A/184856: Polycomb-Associated Non-Coding RNAs.
LF377352 - JP 2014500723-A/184855: Polycomb-Associated Non-Coding RNAs.
MA444105 - JP 2018138019-A/16031: Polycomb-Associated Non-Coding RNAs.
MA612930 - JP 2018138019-A/184856: Polycomb-Associated Non-Coding RNAs.
MA612929 - JP 2018138019-A/184855: Polycomb-Associated Non-Coding RNAs.

-  Biochemical and Signaling Pathways
  BioCyc Knowledge Library
PWY66-400 - glycolysis
PWY66-407 - superpathway of conversion of glucose to acetyl CoA and entry into the TCA cycle

-  Other Names for This Gene
  Alternate Gene Symbols: ENST00000335181.1, ENST00000335181.2, ENST00000335181.3, ENST00000335181.4, ENST00000335181.5, ENST00000335181.6, ENST00000335181.7, ENST00000335181.8, ENST00000335181.9, HEL-S-30 , NM_002654, uc317uen.1, uc317uen.2, V9HWB8, V9HWB8_HUMAN
UCSC ID: ENST00000335181.10_15
RefSeq Accession: NM_002654.6

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.