Human Gene PLA2G15 (ENST00000219345.10_5) from GENCODE V47lift37
  Description: phospholipase A2 group XV, transcript variant 1 (from RefSeq NM_012320.4)
Gencode Transcript: ENST00000219345.10_5
Gencode Gene: ENSG00000103066.13_14
Transcript (Including UTRs)
   Position: hg19 chr16:68,279,276-68,294,961 Size: 15,686 Total Exon Count: 6 Strand: +
Coding Region
   Position: hg19 chr16:68,279,330-68,293,560 Size: 14,231 Coding Exon Count: 6 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr16:68,279,276-68,294,961)mRNA (may differ from genome)Protein (412 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMGIOMIMPubMedReactomeUniProtKB
WikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: PAG15_HUMAN
DESCRIPTION: RecName: Full=Group XV phospholipase A2; EC=2.3.1.-; AltName: Full=1-O-acylceramide synthase; Short=ACS; AltName: Full=LCAT-like lysophospholipase; Short=LLPL; AltName: Full=Lysophospholipase 3; AltName: Full=Lysosomal phospholipase A2; Short=LPLA2; Flags: Precursor;
FUNCTION: Has transacylase and calcium-independent phospholipase A2 activity. Catalyzes the formation of 1-O-acyl-N- acetylsphingosine and the concomitant release of a lyso- phospholipid (By similarity). May have weak lysophospholipase activity.
SUBCELLULAR LOCATION: Lysosome (By similarity). Secreted.
TISSUE SPECIFICITY: Highly expressed in heart, placenta, skeletal muscle, kidney and pancreas. Detected at lower levels in spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes.
PTM: N-glycosylated.
SIMILARITY: Belongs to the AB hydrolase superfamily. Lipase family.
SEQUENCE CAUTION: Sequence=CAB53675.1; Type=Erroneous initiation;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 16.97 RPKM in Kidney - Cortex
Total median expression: 351.12 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -10.0054-0.185 Picture PostScript Text
3' UTR -623.301401-0.445 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR003386 - LACT/PDAT_acylTrfase

Pfam Domains:
PF02450 - Lecithin:cholesterol acyltransferase

SCOP Domains:
53187 - Zn-dependent exopeptidases
53474 - alpha/beta-Hydrolases
55856 - Cytochrome b5-like heme/steroid binding domain

ModBase Predicted Comparative 3D Structure on Q8NCC3
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologGenome BrowserNo ortholog
Gene DetailsGene Details Gene DetailsGene Details 
Gene SorterGene Sorter Gene SorterGene Sorter 
 RGDEnsembl WormBase 
    Protein Sequence 
    Alignment 

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004622 lysophospholipase activity
GO:0005543 phospholipid binding
GO:0008374 O-acyltransferase activity
GO:0016740 transferase activity
GO:0016746 transferase activity, transferring acyl groups
GO:0016787 hydrolase activity
GO:0047499 calcium-independent phospholipase A2 activity

Biological Process:
GO:0006629 lipid metabolic process
GO:0006631 fatty acid metabolic process
GO:0006644 phospholipid metabolic process
GO:0006650 glycerophospholipid metabolic process
GO:0006672 ceramide metabolic process
GO:0009062 fatty acid catabolic process
GO:0016042 lipid catabolic process
GO:0034638 phosphatidylcholine catabolic process
GO:0046338 phosphatidylethanolamine catabolic process
GO:0046470 phosphatidylcholine metabolic process

Cellular Component:
GO:0005576 extracellular region
GO:0005615 extracellular space
GO:0005654 nucleoplasm
GO:0005764 lysosome
GO:0016020 membrane
GO:0043231 intracellular membrane-bounded organelle
GO:0070062 extracellular exosome


-  Descriptions from all associated GenBank mRNAs
  LF208294 - JP 2014500723-A/15797: Polycomb-Associated Non-Coding RNAs.
AK300596 - Homo sapiens cDNA FLJ58244 complete cds, highly similar to 1-O-acylceramide synthase precursor (EC 2.3.1.-).
AK074828 - Homo sapiens cDNA FLJ90347 fis, clone NT2RP2003042, highly similar to Lysophospholipase 3 (lysosomal phospholipase A2).
AK296263 - Homo sapiens cDNA FLJ58003 complete cds, highly similar to 1-O-acylceramide synthase precursor (EC 2.3.1.-).
AK298494 - Homo sapiens cDNA FLJ57757 complete cds, highly similar to 1-O-acylceramide synthase precursor (EC 2.3.1.-).
AL110209 - Homo sapiens mRNA; cDNA DKFZp564A0122 (from clone DKFZp564A0122); partial cds.
BC062605 - Homo sapiens phospholipase A2, group XV, mRNA (cDNA clone MGC:74676 IMAGE:5162981), complete cds.
AK001705 - Homo sapiens cDNA FLJ10843 fis, clone NT2RP4001345, highly similar to 1-O-acylceramide synthase precursor (EC 2.3.1.-).
AK222790 - Homo sapiens mRNA for lysophospholipase 3 (lysosomal phospholipase A2) variant, clone: HEP01344.
AB017494 - Homo sapiens mRNA for LCAT-like lysophospholipase (LLPL), complete cds.
AY358425 - Homo sapiens clone DNA44189 LLPL (UNQ341) mRNA, complete cds.
EU832326 - Synthetic construct Homo sapiens clone HAIB:100067355; DKFZo008E1227 lysophospholipase 3 (lysosomal phospholipase A2) protein (LYPLA3) gene, encodes complete protein.
MA443871 - JP 2018138019-A/15797: Polycomb-Associated Non-Coding RNAs.
JD382435 - Sequence 363459 from Patent EP1572962.
JD064201 - Sequence 45225 from Patent EP1572962.
JD365119 - Sequence 346143 from Patent EP1572962.
LF374211 - JP 2014500723-A/181714: Polycomb-Associated Non-Coding RNAs.
MA609788 - JP 2018138019-A/181714: Polycomb-Associated Non-Coding RNAs.
AK127898 - Homo sapiens cDNA FLJ46004 fis, clone SMINT2012179, highly similar to Homo sapiens lysophospholipase 3 (lysosomal phospholipase A2) (LYPLA3).
BC011640 - Homo sapiens phospholipase A2, group XV, mRNA (cDNA clone IMAGE:4124459), partial cds.
LF374213 - JP 2014500723-A/181716: Polycomb-Associated Non-Coding RNAs.
CU679996 - Synthetic construct Homo sapiens gateway clone IMAGE:100018696 5' read LYPLA3 mRNA.
KJ902362 - Synthetic construct Homo sapiens clone ccsbBroadEn_11756 PLA2G15 gene, encodes complete protein.
LF374215 - JP 2014500723-A/181718: Polycomb-Associated Non-Coding RNAs.
LF374216 - JP 2014500723-A/181719: Polycomb-Associated Non-Coding RNAs.
AL389957 - Homo sapiens mRNA full length insert cDNA clone EUROIMAGE 2004595.
LF374219 - JP 2014500723-A/181722: Polycomb-Associated Non-Coding RNAs.
LF374220 - JP 2014500723-A/181723: Polycomb-Associated Non-Coding RNAs.
JD019677 - Sequence 701 from Patent EP1572962.
LF374221 - JP 2014500723-A/181724: Polycomb-Associated Non-Coding RNAs.
JD032223 - Sequence 13247 from Patent EP1572962.
LF374223 - JP 2014500723-A/181726: Polycomb-Associated Non-Coding RNAs.
JD233096 - Sequence 214120 from Patent EP1572962.
LF374224 - JP 2014500723-A/181727: Polycomb-Associated Non-Coding RNAs.
JD271772 - Sequence 252796 from Patent EP1572962.
JD496514 - Sequence 477538 from Patent EP1572962.
JD550526 - Sequence 531550 from Patent EP1572962.
JD551177 - Sequence 532201 from Patent EP1572962.
JD073665 - Sequence 54689 from Patent EP1572962.
LF374225 - JP 2014500723-A/181728: Polycomb-Associated Non-Coding RNAs.
JD115137 - Sequence 96161 from Patent EP1572962.
JD278110 - Sequence 259134 from Patent EP1572962.
JD136854 - Sequence 117878 from Patent EP1572962.
JD110319 - Sequence 91343 from Patent EP1572962.
JD545936 - Sequence 526960 from Patent EP1572962.
JD401084 - Sequence 382108 from Patent EP1572962.
JD317086 - Sequence 298110 from Patent EP1572962.
JD076071 - Sequence 57095 from Patent EP1572962.
JD022060 - Sequence 3084 from Patent EP1572962.
JD034608 - Sequence 15632 from Patent EP1572962.
JD497218 - Sequence 478242 from Patent EP1572962.
JD217365 - Sequence 198389 from Patent EP1572962.
JD225282 - Sequence 206306 from Patent EP1572962.
JD192858 - Sequence 173882 from Patent EP1572962.
JD214982 - Sequence 196006 from Patent EP1572962.
JD467486 - Sequence 448510 from Patent EP1572962.
JD055855 - Sequence 36879 from Patent EP1572962.
JD277058 - Sequence 258082 from Patent EP1572962.
JD477721 - Sequence 458745 from Patent EP1572962.
JD431775 - Sequence 412799 from Patent EP1572962.
JD115602 - Sequence 96626 from Patent EP1572962.
JD239417 - Sequence 220441 from Patent EP1572962.
JD178776 - Sequence 159800 from Patent EP1572962.
JD464652 - Sequence 445676 from Patent EP1572962.
JD366063 - Sequence 347087 from Patent EP1572962.
LF374228 - JP 2014500723-A/181731: Polycomb-Associated Non-Coding RNAs.
JD072797 - Sequence 53821 from Patent EP1572962.
JD214050 - Sequence 195074 from Patent EP1572962.
JD247093 - Sequence 228117 from Patent EP1572962.
JD465800 - Sequence 446824 from Patent EP1572962.
JD019556 - Sequence 580 from Patent EP1572962.
JD028518 - Sequence 9542 from Patent EP1572962.
JD400920 - Sequence 381944 from Patent EP1572962.
JD158662 - Sequence 139686 from Patent EP1572962.
JD086668 - Sequence 67692 from Patent EP1572962.
MA609790 - JP 2018138019-A/181716: Polycomb-Associated Non-Coding RNAs.
MA609792 - JP 2018138019-A/181718: Polycomb-Associated Non-Coding RNAs.
MA609793 - JP 2018138019-A/181719: Polycomb-Associated Non-Coding RNAs.
MA609796 - JP 2018138019-A/181722: Polycomb-Associated Non-Coding RNAs.
MA609797 - JP 2018138019-A/181723: Polycomb-Associated Non-Coding RNAs.
MA609798 - JP 2018138019-A/181724: Polycomb-Associated Non-Coding RNAs.
MA609800 - JP 2018138019-A/181726: Polycomb-Associated Non-Coding RNAs.
MA609801 - JP 2018138019-A/181727: Polycomb-Associated Non-Coding RNAs.
MA609802 - JP 2018138019-A/181728: Polycomb-Associated Non-Coding RNAs.
MA609805 - JP 2018138019-A/181731: Polycomb-Associated Non-Coding RNAs.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q8NCC3 (Reactome details) participates in the following event(s):

R-HSA-8952251 PLA2G15 hydrolyses LPC to GPCho and LCFA(-)
R-HSA-1483115 Hydrolysis of LPC
R-HSA-1483206 Glycerophospholipid biosynthesis
R-HSA-1483257 Phospholipid metabolism
R-HSA-556833 Metabolism of lipids
R-HSA-1430728 Metabolism

-  Other Names for This Gene
  Alternate Gene Symbols: B3KMF3, B4DUD1, ENST00000219345.1, ENST00000219345.2, ENST00000219345.3, ENST00000219345.4, ENST00000219345.5, ENST00000219345.6, ENST00000219345.7, ENST00000219345.8, ENST00000219345.9, LYPLA3, NM_012320, PAG15_HUMAN, PLA2G15 , Q53GZ1, Q8NCC3, Q9NPQ6, Q9UG04, Q9Y2B3, uc317cxh.1, uc317cxh.2, UNQ341/PRO540
UCSC ID: ENST00000219345.10_5
RefSeq Accession: NM_012320.4
Protein: Q8NCC3 (aka PAG15_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.