Human Gene PLA2G4B (ENST00000458483.4_6) from GENCODE V47lift37
  Description: phospholipase A2 group IVB (from RefSeq NM_001114633.2)
Gencode Transcript: ENST00000458483.4_6
Gencode Gene: ENSG00000243708.11_15
Transcript (Including UTRs)
   Position: hg19 chr15:42,131,045-42,140,341 Size: 9,297 Total Exon Count: 20 Strand: +
Coding Region
   Position: hg19 chr15:42,131,112-42,140,058 Size: 8,947 Coding Exon Count: 20 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr15:42,131,045-42,140,341)mRNA (may differ from genome)Protein (781 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGeneCardsHGNC
MGIOMIMPubMedReactomeUniProtKBBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: PA24B_HUMAN
DESCRIPTION: RecName: Full=Cytosolic phospholipase A2 beta; Short=cPLA2-beta; EC=3.1.1.4; AltName: Full=Phospholipase A2 group IVB;
FUNCTION: Calcium-dependent phospholipase A2 that selectively hydrolyzes glycerophospholipids in the sn-2 position with a preference for arachidonoyl phospholipids. Has a much weaker activity than PLA2G4A. Isoform 3 has calcium-dependent activity against palmitoyl-arachidonyl-phosphatidylethanolamine and low level lysophospholipase activity but no activity against phosphatidylcholine. Isoform 5 does have activity against phosphatidylcholine.
CATALYTIC ACTIVITY: Phosphatidylcholine + H(2)O = 1- acylglycerophosphocholine + a carboxylate.
ENZYME REGULATION: Stimulated by cytosolic Ca(2+).
BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: Vmax=7.9 pmol/min/mg enzyme (isoform 5); Vmax=3.6 pmol/min/ug enzyme with cAMP as substrate (isoform 4); Vmax=140 nmol/min/ug enzyme with 1-[14C]16:0-2-lyso-PC as substrate (isoform 5); Vmax=1.6 nmol/min/ug enzyme with 1-[14C]16:0-2-lyso-PC as substrate (isoform 3); Vmax=2.1 nmol/min/ug enzyme with 1-16:0-2-[14C]20:4-PC as substrate (isoform 5); Vmax=0.6 nmol/min/ug enzyme with 1-16:0-2-[14C]20:4-PE as substrate (isoform 5); Vmax=0.8 nmol/min/ug enzyme with 1-16:0-2-[14C]20:4-PE as substrate (isoform 3); Vmax=0.3 nmol/min/ug enzyme with 1-16:0-2-[14C]18:2-PE as substrate (isoform 3);
SUBCELLULAR LOCATION: Cytoplasm, cytosol.
SUBCELLULAR LOCATION: Isoform 3: Mitochondrion membrane; Peripheral membrane protein. Early endosome membrane; Peripheral membrane protein. Note=Translocates to membrane vesicles in a calcium-dependent fashion.
TISSUE SPECIFICITY: Widely expressed. Expressed at higher level in brain, heart, liver, cerebellum and pancreas. Isoform 3 is widely expressed.
DOMAIN: The N-terminal C2 domain associates with lipid membranes upon calcium binding. It modulates enzyme activity by presenting the active site to its substrate in response to elevations of cytosolic Ca(2+) (By similarity).
SIMILARITY: Contains 1 C2 domain.
SIMILARITY: Contains 1 PLA2c domain.
CAUTION: Most tissues also express read-through transcripts from this gene into the upstream gene (JMJD7), some of which may encode fusion proteins.
SEQUENCE CAUTION: Sequence=BAD92387.1; Type=Erroneous initiation; Note=Translation N-terminally shortened;

-  Primer design for this transcript
 

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Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

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-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 32.22 RPKM in Brain - Cerebellum
Total median expression: 252.46 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -14.7067-0.219 Picture PostScript Text
3' UTR -107.20283-0.379 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR016035 - Acyl_Trfase/lysoPLipase
IPR000008 - C2_Ca-dep
IPR008973 - C2_Ca/lipid-bd_dom_CaLB
IPR018029 - C2_membr_targeting
IPR002642 - LysoPLipase_cat_dom

Pfam Domains:
PF00168 - C2 domain
PF01735 - Lysophospholipase catalytic domain
PF18695 - Cytosolic phospholipases A2 C2-domain

SCOP Domains:
49562 - C2 domain (Calcium/lipid-binding domain, CaLB)
52151 - FabD/lysophospholipase-like

ModBase Predicted Comparative 3D Structure on P0C869
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004620 phospholipase activity
GO:0004622 lysophospholipase activity
GO:0004623 phospholipase A2 activity
GO:0005509 calcium ion binding
GO:0005544 calcium-dependent phospholipid binding
GO:0008970 phosphatidylcholine 1-acylhydrolase activity
GO:0016787 hydrolase activity
GO:0046872 metal ion binding
GO:0047498 calcium-dependent phospholipase A2 activity

Biological Process:
GO:0006629 lipid metabolic process
GO:0006644 phospholipid metabolic process
GO:0006654 phosphatidic acid biosynthetic process
GO:0006954 inflammatory response
GO:0007567 parturition
GO:0008152 metabolic process
GO:0009395 phospholipid catabolic process
GO:0016042 lipid catabolic process
GO:0019369 arachidonic acid metabolic process
GO:0019722 calcium-mediated signaling
GO:0036148 phosphatidylglycerol acyl-chain remodeling
GO:0036150 phosphatidylserine acyl-chain remodeling
GO:0036151 phosphatidylcholine acyl-chain remodeling
GO:0036152 phosphatidylethanolamine acyl-chain remodeling
GO:0036498 IRE1-mediated unfolded protein response
GO:0046475 glycerophospholipid catabolic process

Cellular Component:
GO:0005576 extracellular region
GO:0005737 cytoplasm
GO:0005739 mitochondrion
GO:0005743 mitochondrial inner membrane
GO:0005768 endosome
GO:0005829 cytosol
GO:0016020 membrane
GO:0031901 early endosome membrane
GO:0031966 mitochondrial membrane


-  Descriptions from all associated GenBank mRNAs
  BC172355 - Synthetic construct Homo sapiens clone IMAGE:100069049, MGC:199060 JMJD7-PLA2G4B readthrough transcript (JMJD7-PLA2G4B) mRNA, encodes complete protein.
AB209150 - Homo sapiens mRNA for phospholipase A2, group IVB variant protein.
AF065215 - Homo sapiens cytosolic phospholipase A2 beta (cPLA2 beta) mRNA, complete cds.
AF065216 - Homo sapiens cytosolic phospholipase A2 beta (cPLA2 beta) precursor RNA, complete sequence.
DQ523799 - Homo sapiens group IVB cytosolic phospholipase A2 beta splice variant 2 mRNA, complete cds, alternatively spliced.
DQ523800 - Homo sapiens group IVB cytosolic phospholipase A2 beta splice variant 3 mRNA, complete cds, alternatively spliced.
AF121908 - Homo sapiens cytosolic phospholipase A2 beta mRNA, complete cds.
BC013415 - Homo sapiens phospholipase A2, group IVB (cytosolic), mRNA (cDNA clone IMAGE:3528417), partial cds.
AK310651 - Homo sapiens cDNA, FLJ17693.
AK299419 - Homo sapiens cDNA FLJ53173 complete cds, highly similar to Cytosolic phospholipase A2 beta (EC 3.1.1.4).
AK124489 - Homo sapiens cDNA FLJ42498 fis, clone BRACE2035381, highly similar to Homo sapiens phospholipase A2, group IVB (cytosolic) (PLA2G4B).
BX648318 - Homo sapiens mRNA; cDNA DKFZp686N12131 (from clone DKFZp686N12131).
JD392688 - Sequence 373712 from Patent EP1572962.
JD393259 - Sequence 374283 from Patent EP1572962.
JD454037 - Sequence 435061 from Patent EP1572962.
JD394235 - Sequence 375259 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  BioCyc Knowledge Library
LIPASYN-PWY - phospholipases

Reactome (by CSHL, EBI, and GO)

Protein P0C869 (Reactome details) participates in the following event(s):

R-HSA-1482604 PA is hydrolyzed to 1-acyl LPA by PLA2[1] (OM)
R-HSA-1482656 PA is hydrolysed to 1-acyl LPA by PLA2[1]
R-HSA-1482900 PG is hydrolyzed to 1-acyl LPG by PLA2[1]
R-HSA-1482612 2-acyl LPC is hydrolyzed to GPCho by PLA2[8]
R-HSA-1482685 1-acyl LPC is hydrolyzed to GPCho by PLA2[8]
R-HSA-1482862 PC is hydrolysed to 2-acyl LPC by PLA2[7]
R-HSA-1482856 PC is hydrolyzed to 1-acyl LPC by PLA2[5]
R-HSA-1482884 PE is hydrolyzed to 1-acyl LPE by PLA2[2]
R-HSA-1482771 PS is hydrolyzed to 1-acyl LPS by PLA2[9]
R-HSA-1482745 PG is hydrolyzed to 1-acyl LPG by PLA2G4B (IM)
R-HSA-1482847 PG is hydrolysed to 2-acyl LPG by PLA2G4B (IM)
R-HSA-381038 XBP1(S) activates chaperone genes
R-HSA-381070 IRE1alpha activates chaperones
R-HSA-1483166 Synthesis of PA
R-HSA-1482925 Acyl chain remodelling of PG
R-HSA-1483115 Hydrolysis of LPC
R-HSA-1482788 Acyl chain remodelling of PC
R-HSA-1482839 Acyl chain remodelling of PE
R-HSA-1482801 Acyl chain remodelling of PS
R-HSA-381119 Unfolded Protein Response (UPR)
R-HSA-1483206 Glycerophospholipid biosynthesis
R-HSA-392499 Metabolism of proteins
R-HSA-1483257 Phospholipid metabolism
R-HSA-556833 Metabolism of lipids
R-HSA-1430728 Metabolism

-  Other Names for This Gene
  Alternate Gene Symbols: B4DRT9, ENST00000458483.1, ENST00000458483.2, ENST00000458483.3, NM_001114633, O95712, P0C869, PA24B_HUMAN, Q19KD5, Q19KD6, Q59GF9, Q8TB10, Q9UKV7, uc320zhh.1, uc320zhh.2
UCSC ID: ENST00000458483.4_6
RefSeq Accession: NM_001114633.2
Protein: P0C869 (aka PA24B_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.