Human Gene PLAU (ENST00000372764.4_7) from GENCODE V47lift37
  Description: plasminogen activator, urokinase, transcript variant 1 (from RefSeq NM_002658.6)
Gencode Transcript: ENST00000372764.4_7
Gencode Gene: ENSG00000122861.16_10
Transcript (Including UTRs)
   Position: hg19 chr10:75,670,890-75,677,252 Size: 6,363 Total Exon Count: 11 Strand: +
Coding Region
   Position: hg19 chr10:75,671,314-75,676,323 Size: 5,010 Coding Exon Count: 10 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
Gene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther Species
GO AnnotationsmRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr10:75,670,890-75,677,252)mRNA (may differ from genome)Protein (431 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCHuman Cortex Gene ExpressionMalacardsMGIOMIMPubMed
ReactomeUniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: UROK_HUMAN
DESCRIPTION: RecName: Full=Urokinase-type plasminogen activator; Short=U-plasminogen activator; Short=uPA; EC=3.4.21.73; Contains: RecName: Full=Urokinase-type plasminogen activator long chain A; Contains: RecName: Full=Urokinase-type plasminogen activator short chain A; Contains: RecName: Full=Urokinase-type plasminogen activator chain B; Flags: Precursor;
FUNCTION: Specifically cleave the zymogen plasminogen to form the active enzyme plasmin.
CATALYTIC ACTIVITY: Specific cleavage of Arg-|-Val bond in plasminogen to form plasmin.
ENZYME REGULATION: Inhibited by SERPINA5.
SUBUNIT: Found in high and low molecular mass forms. Each consists of two chains, A and B. The high molecular mass form contains a long chain A which is cleaved to yield a short chain A. Forms heterodimer with SERPINA5. Binds LRP1B; binding is followed by internalization and degradation. Interacts with MRC2. Interacts with PLAUR.
SUBCELLULAR LOCATION: Secreted.
TISSUE SPECIFICITY: Expressed in the prostate gland and prostate cancers.
PTM: Phosphorylation of Ser-158 and Ser-323 abolishes proadhesive ability but does not interfere with receptor binding.
DISEASE: Defects in PLAU are the cause of Quebec platelet disorder (QPD) [MIM:601709]. QPD is an autosomal dominant bleeding disorder due to a gain-of-function defect in fibrinolysis. Although affected individuals do not exhibit systemic fibrinolysis, they show delayed onset bleeding after challenge, such as surgery. The hallmark of the disorder is markedly increased PLAU levels within platelets, which causes intraplatelet plasmin generation and secondary degradation of alpha-granule proteins.
PHARMACEUTICAL: Available under the name Abbokinase (Abbott). Used in Pulmonary Embolism (PE) to initiate fibrinolysis. Clinically used for therapy of thrombolytic disorders.
SIMILARITY: Belongs to the peptidase S1 family.
SIMILARITY: Contains 1 EGF-like domain.
SIMILARITY: Contains 1 kringle domain.
SIMILARITY: Contains 1 peptidase S1 domain.
WEB RESOURCE: Name=Wikipedia; Note=Urokinase entry; URL="http://en.wikipedia.org/wiki/Urokinase";
WEB RESOURCE: Name=SeattleSNPs; URL="http://pga.gs.washington.edu/data/plau/";

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: PLAU
Diseases sorted by gene-association score: quebec platelet disorder* (1400), factor v deficiency* (283), alzheimer disease* (256), ureter cancer (19), angiostrongyliasis (13), eosinophilic meningitis (11), endometrial adenosquamous carcinoma (11), perforated corneal ulcer (10), cornea cancer (10), intratubular embryonal carcinoma (10), conjunctivochalasis (9), buerger disease (8), paroxysmal nocturnal hemoglobinuria (8), ovarian cyst (8), fibrosarcoma (8), joint disorders (7), abdominal aortic aneurysm (7), paranasal sinus disease (6), chronic meningitis (5), breast adenocarcinoma (5), colorectal cancer (4), stomach cancer (4), ovarian cancer, somatic (4), lung cancer (4), prostate cancer (4), alzheimer disease-2 (3), breast cancer (3), pancreatic cancer (3), kallmann syndrome (1), combined t cell and b cell immunodeficiency (1)
* = Manually curated disease association

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 24.82 RPKM in Adipose - Visceral (Omentum)
Total median expression: 282.98 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -40.90118-0.347 Picture PostScript Text
3' UTR -327.10929-0.352 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR000742 - EG-like_dom
IPR013032 - EGF-like_CS
IPR000001 - Kringle
IPR013806 - Kringle-like
IPR018056 - Kringle_CS
IPR009003 - Pept_cys/ser_Trypsin-like
IPR018114 - Peptidase_S1/S6_AS
IPR001254 - Peptidase_S1_S6
IPR001314 - Peptidase_S1A

Pfam Domains:
PF00051 - Kringle domain
PF00089 - Trypsin

SCOP Domains:
50494 - Trypsin-like serine proteases
57440 - Kringle-like
57196 - EGF/Laminin

Protein Data Bank (PDB) 3-D Structure
MuPIT help
1C5W - X-ray MuPIT 1C5X - X-ray MuPIT 1C5Y - X-ray MuPIT 1C5Z - X-ray MuPIT 1EJN - X-ray MuPIT 1F5K - X-ray MuPIT 1F5L - X-ray MuPIT 1F92 - X-ray MuPIT 1FV9 - X-ray MuPIT 1GI7 - X-ray MuPIT 1GI8 - X-ray MuPIT 1GI9 - X-ray MuPIT 1GJ7 - X-ray MuPIT 1GJ8 - X-ray MuPIT 1GJ9 - X-ray MuPIT 1GJA - X-ray MuPIT 1GJB - X-ray MuPIT 1GJC - X-ray MuPIT 1GJD - X-ray MuPIT 1KDU - NMR MuPIT 1LMW - X-ray MuPIT 1O3P - X-ray MuPIT 1O5A - X-ray MuPIT 1O5B - X-ray MuPIT 1O5C - X-ray MuPIT 1OWD - X-ray MuPIT 1OWE - X-ray MuPIT 1OWH - X-ray MuPIT 1OWI - X-ray MuPIT 1OWJ - X-ray MuPIT 1OWK - X-ray MuPIT 1SC8 - X-ray MuPIT 1SQA - X-ray MuPIT 1SQO - X-ray MuPIT 1SQT - X-ray MuPIT 1U6Q - X-ray MuPIT 1URK - NMR MuPIT 1VJ9 - X-ray MuPIT 1VJA - X-ray MuPIT 1W0Z - X-ray MuPIT 1W10 - X-ray MuPIT 1W11 - X-ray MuPIT 1W12 - X-ray MuPIT 1W13 - X-ray MuPIT 1W14 - X-ray MuPIT 2FD6 - X-ray MuPIT 2I9A - X-ray 2I9B - X-ray 2NWN - X-ray MuPIT 2O8T - X-ray MuPIT 2O8U - X-ray MuPIT 2O8W - X-ray MuPIT 2R2W - X-ray MuPIT 2VIN - X-ray MuPIT 2VIO - X-ray MuPIT 2VIP - X-ray MuPIT 2VIQ - X-ray MuPIT 2VIV - X-ray MuPIT 2VIW - X-ray MuPIT 2VNT - X-ray MuPIT 3BT1 - X-ray MuPIT 3BT2 - X-ray MuPIT 3IG6 - X-ray MuPIT 3KGP - X-ray MuPIT 3KHV - X-ray MuPIT 3KID - X-ray MuPIT 3M61 - X-ray MuPIT 3MHW - X-ray MuPIT 3MWI - X-ray MuPIT 3OX7 - X-ray MuPIT 3OY5 - X-ray MuPIT 3OY6 - X-ray MuPIT 3PB1 - X-ray MuPIT 3QN7 - X-ray MuPIT 3U73 - X-ray MuPIT 4FU7 - X-ray MuPIT 4FU8 - X-ray MuPIT 4FU9 - X-ray MuPIT 4FUB - X-ray MuPIT 4FUC - X-ray MuPIT 4FUD - X-ray MuPIT 4FUE - X-ray MuPIT 4FUF - X-ray MuPIT 4FUG - X-ray MuPIT 4FUH - X-ray MuPIT 4FUI - X-ray MuPIT 4FUJ - X-ray MuPIT


ModBase Predicted Comparative 3D Structure on P00749
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
Gene SorterGene Sorter    
 RGDEnsembl   
      
      

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004252 serine-type endopeptidase activity
GO:0005515 protein binding
GO:0008233 peptidase activity
GO:0008236 serine-type peptidase activity
GO:0016787 hydrolase activity

Biological Process:
GO:0001666 response to hypoxia
GO:0006508 proteolysis
GO:0006935 chemotaxis
GO:0007165 signal transduction
GO:0007596 blood coagulation
GO:0007599 hemostasis
GO:0010469 regulation of receptor activity
GO:0014909 smooth muscle cell migration
GO:0014910 regulation of smooth muscle cell migration
GO:0030335 positive regulation of cell migration
GO:0031639 plasminogen activation
GO:0033628 regulation of cell adhesion mediated by integrin
GO:0042127 regulation of cell proliferation
GO:0042730 fibrinolysis
GO:0043312 neutrophil degranulation
GO:0061041 regulation of wound healing
GO:2000097 regulation of smooth muscle cell-matrix adhesion

Cellular Component:
GO:0005576 extracellular region
GO:0005615 extracellular space
GO:0005886 plasma membrane
GO:0005925 focal adhesion
GO:0009986 cell surface
GO:0035579 specific granule membrane
GO:0070062 extracellular exosome
GO:0070821 tertiary granule membrane


-  Descriptions from all associated GenBank mRNAs
  LF212558 - JP 2014500723-A/20061: Polycomb-Associated Non-Coding RNAs.
AK312960 - Homo sapiens cDNA, FLJ93417, highly similar to Homo sapiens plasminogen activator, urokinase (PLAU), mRNA.
AK298560 - Homo sapiens cDNA FLJ57137 complete cds, highly similar to Urokinase-type plasminogen activator precursor (EC 3.4.21.73).
AK297940 - Homo sapiens cDNA FLJ53742 complete cds, highly similar to Urokinase-type plasminogen activator precursor (EC 3.4.21.73).
LF209952 - JP 2014500723-A/17455: Polycomb-Associated Non-Coding RNAs.
BC013575 - Homo sapiens plasminogen activator, urokinase, mRNA (cDNA clone MGC:9223 IMAGE:3890980), complete cds.
A18397 - Human uPA cDNA.
E00924 - cDNA encoding human pro-urokinase.
E01238 - cDNA encoding human pre prourokinase.
E01559 - cDNA sequence coding for human prepro-urokinese.
E01580 - cDNA encoding human pro-urokinase.
E02114 - cDNA sequence coding for human prourokinase.
AH007073 - Homo sapiens chromosome 10 preprourokinase (PLAU) mRNA, complete cds.
E01560 - cDNA sequence coding for human mature urokinase and its.
E00178 - cDNA encoding human urokinase.
M15476 - Human pro-urokinase mRNA, complete cds.
D00244 - Homo sapiens mRNA for pro-urokinase precursor, complete cds.
E01467 - DNA encoding human prourokinase.
LF213792 - JP 2014500723-A/21295: Polycomb-Associated Non-Coding RNAs.
A35395 - H.sapiens u-PA cDNA sequence.
X02760 - Human mRNA for urokinase (EC 3.4.99.26).
AY029537 - Homo sapiens urokinase-type plasminogen activator amino-terminal fragment (ATF) mRNA, complete cds.
DQ893817 - Synthetic construct Homo sapiens clone IMAGE:100008277; FLH164685.01L; RZPDo839A09157D plasminogen activator, urokinase (PLAU) gene, encodes complete protein.
AB528526 - Synthetic construct DNA, clone: pF1KB6608, Homo sapiens PLAU gene for plasminogen activator, urokinase, without stop codon, in Flexi system.
CU680620 - Synthetic construct Homo sapiens gateway clone IMAGE:100017252 5' read PLAU mRNA.
KJ897341 - Synthetic construct Homo sapiens clone ccsbBroadEn_06735 PLAU gene, encodes complete protein.
KJ905272 - Synthetic construct Homo sapiens clone ccsbBroadEn_14769 PLAU gene, encodes complete protein.
KR709719 - Synthetic construct Homo sapiens clone CCSBHm_00005345 PLAU (PLAU) mRNA, encodes complete protein.
E01603 - DNA sequence coding for epidermal growth factor domain of human prourokinase.
E01604 - DNA sequence coding for epidermal growth factor domain of modified human prourokinase.
BT007391 - Homo sapiens plasminogen activator, urokinase mRNA, complete cds.
E02577 - DNA encoding human prourokinase.
E02647 - DNA sequence coding for pro-urokinase.
E06063 - DNA encoding human urokinase derivative.
E06064 - DNA encoding human urokinase.
E00421 - cDNA coding urokinase and its flanking region.
AY820134 - Homo sapiens urokinase plasminogen activator mRNA, partial cds.
D11143 - Homo sapiens upa mRNA for urokinase-type plasminogen activater, partial cds.
E02493 - cDNA encoding precursor of plasminogen activator.
E02832 - DNA encoding human pro-urokinase.
E02833 - DNA encoding human pro-urokinase.
E03359 - cDNA encoding human prourokinase.
LF333157 - JP 2014500723-A/140660: Polycomb-Associated Non-Coding RNAs.
LF333158 - JP 2014500723-A/140661: Polycomb-Associated Non-Coding RNAs.
AB208961 - Homo sapiens mRNA for urokinase plasminogen activator preproprotein variant protein.
LF333159 - JP 2014500723-A/140662: Polycomb-Associated Non-Coding RNAs.
LF333160 - JP 2014500723-A/140663: Polycomb-Associated Non-Coding RNAs.
LF333161 - JP 2014500723-A/140664: Polycomb-Associated Non-Coding RNAs.
Z36790 - H.sapiens (xs139) mRNA, 375bp.
LF333162 - JP 2014500723-A/140665: Polycomb-Associated Non-Coding RNAs.
LF333163 - JP 2014500723-A/140666: Polycomb-Associated Non-Coding RNAs.
JD466903 - Sequence 447927 from Patent EP1572962.
JD477530 - Sequence 458554 from Patent EP1572962.
LF333164 - JP 2014500723-A/140667: Polycomb-Associated Non-Coding RNAs.
JD347806 - Sequence 328830 from Patent EP1572962.
JD464352 - Sequence 445376 from Patent EP1572962.
JD089674 - Sequence 70698 from Patent EP1572962.
JD469357 - Sequence 450381 from Patent EP1572962.
LF333165 - JP 2014500723-A/140668: Polycomb-Associated Non-Coding RNAs.
JD205466 - Sequence 186490 from Patent EP1572962.
JD061303 - Sequence 42327 from Patent EP1572962.
JD229076 - Sequence 210100 from Patent EP1572962.
JD526565 - Sequence 507589 from Patent EP1572962.
JD438431 - Sequence 419455 from Patent EP1572962.
JD051738 - Sequence 32762 from Patent EP1572962.
JD156141 - Sequence 137165 from Patent EP1572962.
JD495386 - Sequence 476410 from Patent EP1572962.
LF333166 - JP 2014500723-A/140669: Polycomb-Associated Non-Coding RNAs.
JD099000 - Sequence 80024 from Patent EP1572962.
JD110378 - Sequence 91402 from Patent EP1572962.
LF333167 - JP 2014500723-A/140670: Polycomb-Associated Non-Coding RNAs.
MA448135 - JP 2018138019-A/20061: Polycomb-Associated Non-Coding RNAs.
MA445529 - JP 2018138019-A/17455: Polycomb-Associated Non-Coding RNAs.
MA449369 - JP 2018138019-A/21295: Polycomb-Associated Non-Coding RNAs.
MA568734 - JP 2018138019-A/140660: Polycomb-Associated Non-Coding RNAs.
MA568735 - JP 2018138019-A/140661: Polycomb-Associated Non-Coding RNAs.
MA568736 - JP 2018138019-A/140662: Polycomb-Associated Non-Coding RNAs.
MA568737 - JP 2018138019-A/140663: Polycomb-Associated Non-Coding RNAs.
MA568738 - JP 2018138019-A/140664: Polycomb-Associated Non-Coding RNAs.
MA568739 - JP 2018138019-A/140665: Polycomb-Associated Non-Coding RNAs.
MA568740 - JP 2018138019-A/140666: Polycomb-Associated Non-Coding RNAs.
MA568741 - JP 2018138019-A/140667: Polycomb-Associated Non-Coding RNAs.
MA568742 - JP 2018138019-A/140668: Polycomb-Associated Non-Coding RNAs.
MA568743 - JP 2018138019-A/140669: Polycomb-Associated Non-Coding RNAs.
MA568744 - JP 2018138019-A/140670: Polycomb-Associated Non-Coding RNAs.

-  Biochemical and Signaling Pathways
  BioCarta from NCI Cancer Genome Anatomy Project
h_fibrinolysisPathway - Fibrinolysis Pathway
h_plateletAppPathway - Platelet Amyloid Precursor Protein Pathway

Reactome (by CSHL, EBI, and GO)

Protein P00749 (Reactome details) participates in the following event(s):

R-HSA-158959 urokinase plasminogen activator + urokinase plasminogen activator receptor (uPAR) -> urokinase plasminogen activator:uPAR
R-HSA-158942 urokinase plasminogen activator (one-chain):uPAR -> urokinase plasminogen activator (two-chain):uPAR
R-HSA-6799350 Exocytosis of specific granule membrane proteins
R-HSA-6798747 Exocytosis of tertiary granule membrane proteins
R-HSA-158982 plasminogen:histidine-rich glycoprotein -> plasmin + histidine-rich glycoprotein (uPA [one-chain] catalyst)
R-HSA-159001 urokinase plasminogen activator (two-chain):uPAR + plasminogen activator inhibitor 2 (PAI-2) -> PAI-2:urokinase plasminogen activator (two-chain):uPAR
R-HSA-159005 urokinase plasminogen activator (two-chain):uPAR + plasminogen activator inhibitor 1 (PAI-1) -> PAI-1:urokinase plasminogen activator (two-chain):uPAR
R-HSA-158925 plasminogen:histidine-rich glycoprotein -> plasmin + histidine-rich glycoprotein (uPA [two-chain] catalyst)
R-HSA-75205 Dissolution of Fibrin Clot
R-HSA-6798695 Neutrophil degranulation
R-HSA-109582 Hemostasis
R-HSA-168249 Innate Immune System
R-HSA-168256 Immune System

-  Other Names for This Gene
  Alternate Gene Symbols: B4DPZ2, ENST00000372764.1, ENST00000372764.2, ENST00000372764.3, NM_002658, P00749, PLAU , Q15844, Q16618, Q53XS3, Q5SWW9, Q969W6, uc318ker.1, uc318ker.2, UROK_HUMAN
UCSC ID: ENST00000372764.4_7
RefSeq Accession: NM_002658.6
Protein: P00749 (aka UROK_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.