Human Gene PNOC (ENST00000301908.8_5) from GENCODE V47lift37
  Description: prepronociceptin, transcript variant 1 (from RefSeq NM_006228.5)
Gencode Transcript: ENST00000301908.8_5
Gencode Gene: ENSG00000168081.9_8
Transcript (Including UTRs)
   Position: hg19 chr8:28,174,785-28,200,868 Size: 26,084 Total Exon Count: 4 Strand: +
Coding Region
   Position: hg19 chr8:28,186,675-28,196,961 Size: 10,287 Coding Exon Count: 2 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
Gene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther Species
GO AnnotationsmRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr8:28,174,785-28,200,868)mRNA (may differ from genome)Protein (176 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCHuman Cortex Gene ExpressionMalacardsMGIOMIMPubMed
ReactomeUniProtKBBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: PNOC_HUMAN
DESCRIPTION: RecName: Full=Prepronociceptin; Contains: RecName: Full=Neuropeptide 1; Contains: RecName: Full=Nociceptin; AltName: Full=Orphanin FQ; AltName: Full=PPNOC; Contains: RecName: Full=Neuropeptide 2; Flags: Precursor;
FUNCTION: Nociceptin is the ligand of the opioid receptor-like receptor (OPRL1). It may act as a transmitter in the brain by modulating nociceptive and locomotor behavior. May be involved in neuronal differentiation and development (By similarity).
SUBCELLULAR LOCATION: Secreted.
TISSUE SPECIFICITY: Predominantly expressed in the brain and spinal cord. Also expressed and secreted by peripheral blood neutrophils following degranulation.
PTM: Specific enzymatic cleavages at paired basic residues probably yield other active peptides besides nociceptin.
PTM: The N-terminal domain contains 6 conserved cysteines thought to be involved in disulfide bonding and/or processing.
SIMILARITY: Belongs to the opioid neuropeptide precursor family.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: PNOC
Diseases sorted by gene-association score: pain agnosia (2), agnosia (2), alcohol dependence (2), specific developmental disorder (1)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene
  • D013749 Tetrachlorodibenzodioxin
  • D015632 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine
  • D015655 1-Methyl-4-phenylpyridinium
  • C111118 2',3,3',4',5-pentachloro-4-hydroxybiphenyl
  • C548651 2-(1'H-indolo-3'-carbonyl)thiazole-4-carboxylic acid methyl ester
  • D000643 Ammonium Chloride
  • D001285 Atropine
  • C066892 CP 96345
  • D002118 Calcium
  • D004052 Diethylnitrosamine
          more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 24.87 RPKM in Cells - EBV-transformed lymphocytes
Total median expression: 55.23 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
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-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -24.7072-0.343 Picture PostScript Text
3' UTR -121.60457-0.266 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR002367 - Nociceptin
IPR006024 - Opioid_neupept

Pfam Domains:
PF01160 - Vertebrate endogenous opioids neuropeptide

ModBase Predicted Comparative 3D Structure on Q13519
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
Gene SorterGene Sorter    
 RGDEnsembl   
      
      

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0001515 opioid peptide activity
GO:0005184 neuropeptide hormone activity
GO:0005515 protein binding
GO:0031628 opioid receptor binding

Biological Process:
GO:0007165 signal transduction
GO:0007186 G-protein coupled receptor signaling pathway
GO:0007218 neuropeptide signaling pathway
GO:0007268 chemical synaptic transmission
GO:0007565 female pregnancy
GO:0007600 sensory perception
GO:0010469 regulation of receptor activity

Cellular Component:
GO:0005576 extracellular region
GO:0005886 plasma membrane
GO:0030425 dendrite
GO:0043025 neuronal cell body
GO:0043679 axon terminus


-  Descriptions from all associated GenBank mRNAs
  U48263 - Human pre-pro-orphanin FQ (OFQ) mRNA, complete cds.
AK289633 - Homo sapiens cDNA FLJ75574 complete cds, highly similar to Homo sapiens prepronociceptin (PNOC), mRNA.
AY335948 - Homo sapiens granulocyte prepronociceptin mRNA, partial cds.
BC034758 - Homo sapiens prepronociceptin, mRNA (cDNA clone MGC:34224 IMAGE:5195392), complete cds.
JD370039 - Sequence 351063 from Patent EP1572962.
X97370 - H.sapiens mRNA for prepronociceptin.
DQ894480 - Synthetic construct Homo sapiens clone IMAGE:100008940; FLH170828.01L; RZPDo839C0299D prepronociceptin (PNOC) gene, encodes complete protein.
DQ891296 - Synthetic construct clone IMAGE:100003926; FLH170832.01X; RZPDo839C02100D prepronociceptin (PNOC) gene, encodes complete protein.
CU689960 - Synthetic construct Homo sapiens gateway clone IMAGE:100020622 5' read PNOC mRNA.
KJ891832 - Synthetic construct Homo sapiens clone ccsbBroadEn_01226 PNOC gene, encodes complete protein.
CR541965 - Homo sapiens full open reading frame cDNA clone RZPDo834G0734D for gene PNOC, prepronociceptin; complete cds, without stopcodon.
BT019334 - Homo sapiens prepronociceptin mRNA, complete cds.
CR541940 - Homo sapiens full open reading frame cDNA clone RZPDo834A0834D for gene PNOC, prepronociceptin; complete cds, incl. stopcodon.
AK301456 - Homo sapiens cDNA FLJ50894 complete cds, highly similar to Nociceptin precursor.
JD217557 - Sequence 198581 from Patent EP1572962.
JD422365 - Sequence 403389 from Patent EP1572962.
JD369204 - Sequence 350228 from Patent EP1572962.
JD362998 - Sequence 344022 from Patent EP1572962.
JD259302 - Sequence 240326 from Patent EP1572962.
JD423480 - Sequence 404504 from Patent EP1572962.
JD082660 - Sequence 63684 from Patent EP1572962.
JD251689 - Sequence 232713 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q13519 (Reactome details) participates in the following event(s):

R-HSA-374723 Nociceptin receptor binds to nociceptin
R-HSA-749454 The Ligand:GPCR:Gi complex dissociates
R-HSA-749456 Liganded Gi-activating GPCRs bind inactive heterotrimeric G-protein Gi
R-HSA-380073 Liganded Gi-activating GPCR acts as a GEF for Gi
R-HSA-375276 Peptide ligand-binding receptors
R-HSA-418594 G alpha (i) signalling events
R-HSA-373076 Class A/1 (Rhodopsin-like receptors)
R-HSA-388396 GPCR downstream signalling
R-HSA-500792 GPCR ligand binding
R-HSA-372790 Signaling by GPCR
R-HSA-162582 Signal Transduction

-  Other Names for This Gene
  Alternate Gene Symbols: B7Z749, ENST00000301908.1, ENST00000301908.2, ENST00000301908.3, ENST00000301908.4, ENST00000301908.5, ENST00000301908.6, ENST00000301908.7, NM_006228, OFQ, PNOC_HUMAN, Q13519, Q6FH16, uc317mxm.1, uc317mxm.2
UCSC ID: ENST00000301908.8_5
RefSeq Accession: NM_006228.5
Protein: Q13519 (aka PNOC_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.