ID:DPOD1_HUMAN DESCRIPTION: RecName: Full=DNA polymerase delta catalytic subunit; EC=2.7.7.7; AltName: Full=DNA polymerase subunit delta p125; FUNCTION: Possesses two enzymatic activities: DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3'- to 5'-direction. Required with its accessory proteins (proliferating cell nuclear antigen (PCNA) and replication factor C (RFC) or activator 1) for leading strand synthesis. Also involved in completing Okazaki fragments initiated by the DNA polymerase alpha/primase complex. CATALYTIC ACTIVITY: Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). COFACTOR: Binds 1 4Fe-4S cluster (By similarity). SUBUNIT: Heterotetramer composed of subunits of 125 kDa, 50 kDa, 66 kDa and 12 kDa. The 125 kDa subunit contains the polymerase active site and most likely the active site for the 3'-5' exonuclease activity. Interacts with WRNIP1. Interacts with POLD4 and PCNA. INTERACTION: P12004:PCNA; NbExp=2; IntAct=EBI-716569, EBI-358311; P49005:POLD2; NbExp=9; IntAct=EBI-716569, EBI-372354; Q9HCU8:POLD4; NbExp=10; IntAct=EBI-716569, EBI-864968; SUBCELLULAR LOCATION: Nucleus. DOMAIN: The CysB motif binds 1 4Fe-4S cluster and is required for the formation of polymerase complexes (By similarity). MISCELLANEOUS: In eukaryotes there are five DNA polymerases: alpha, beta, gamma, delta, and epsilon which are responsible for different reactions of DNA synthesis. SIMILARITY: Belongs to the DNA polymerase type-B family. SIMILARITY: Contains 1 CysA-type zinc finger. WEB RESOURCE: Name=NIEHS-SNPs; URL="http://egp.gs.washington.edu/data/pold1/";
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
Pfam Domains: PF00136 - DNA polymerase family B PF03104 - DNA polymerase family B, exonuclease domain PF14260 - C4-type zinc-finger of DNA polymerase delta
ModBase Predicted Comparative 3D Structure on P28340
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
Biological Process: GO:0000723 telomere maintenance GO:0000731 DNA synthesis involved in DNA repair GO:0006260 DNA replication GO:0006281 DNA repair GO:0006283 transcription-coupled nucleotide-excision repair GO:0006287 base-excision repair, gap-filling GO:0006296 nucleotide-excision repair, DNA incision, 5'-to lesion GO:0006297 nucleotide-excision repair, DNA gap filling GO:0006974 cellular response to DNA damage stimulus GO:0009411 response to UV GO:0019985 translesion synthesis GO:0032201 telomere maintenance via semi-conservative replication GO:0033683 nucleotide-excision repair, DNA incision GO:0034644 cellular response to UV GO:0042769 DNA damage response, detection of DNA damage GO:0045004 DNA replication proofreading GO:0055089 fatty acid homeostasis
Protein P28340 (Reactome details) participates in the following event(s):
R-HSA-69074 Formation of Processive Complex R-HSA-174448 Formation of Processive Complex on the C-strand of the telomere R-HSA-176702 Disassociation of Processive Complex and Completed Telomere End R-HSA-69116 Formation of Okazaki fragments R-HSA-174445 RPA binds to the Flap on the C-strand R-HSA-174438 Formation of the Flap Intermediate on the C-strand R-HSA-174456 Joining of adjacent Okazaki fragments of the C-strand R-HSA-174446 Removal of remaining Flap from the C-strand R-HSA-174444 Formation of C-strand Okazaki fragments R-HSA-174441 Removal of RNA primer and dissociation of RPA and Dna2 from the C-strand R-HSA-5653838 POLD,POLE binds deISGylated PCNA after TLS R-HSA-5652009 RAD18:UBE2B or RBX1:CUL4:DDB1:DTL monoubiquitinates PCNA R-HSA-5358579 DNA polymerase delta polymerizes DNA across single stranded gap R-HSA-69127 Formation of the Flap Intermediate R-HSA-69152 Removal of remaining Flap R-HSA-69144 Removal of RNA primer and dissociation of RPA and Dna2 R-HSA-174451 Recruitment of Dna2 endonuclease to the C strand R-HSA-110364 PCNA:POLD,POLE:RPA:RFC and FEN1 bind APEX1 R-HSA-5651992 PCNA-containing replication complex binds damaged dsDNA R-HSA-5651809 LIG1, APEX1 and PCNA:POLD,POLE:RPA:RFC dissociate from repaired DNA R-HSA-5653840 POLD,POLE complete replication of damaged DNA after TLS R-HSA-5690213 DNA polymerases delta, epsilon or kappa bind the GG-NER site R-HSA-6782211 DNA polymerases delta, epsilon or kappa bind the TC-NER site R-HSA-69140 RPA binds to the Flap R-HSA-110368 POLD,POLE-mediated DNA strand displacement synthesis R-HSA-110371 LIG1 binds APEX1 and PCNA at SSB R-HSA-110363 FEN1 bound to PCNA and APEX1 cleaves flap ssDNA R-HSA-5651805 LIG1 bound to APEX1 and PCNA ligates SSB R-HSA-5652005 RAD18:UBE2B or RBX1:CUL4:DDB1:DTL ubiquitin ligase complex binds PCNA:POLD,POLE:RPA:RFC associated with damaged dsDNA R-HSA-69142 Recruitment of Dna2 endonuclease R-HSA-5693593 D-loop extension by DNA polymerases R-HSA-5690997 Ligation of newly synthesized repair patch to incised DNA in GG-NER R-HSA-6782227 Ligation of newly synthesized repair patch to incised DNA in TC-NER R-HSA-5690988 3'-incision of DNA by ERCC5 (XPG) in GG-NER R-HSA-5691001 Repair DNA synthesis of ~27-30 bases long patch by POLD, POLE or POLK in GG-NER R-HSA-6782224 3' incision by ERCC5 (XPG) in TC-NER R-HSA-6782208 Repair DNA synthesis of ~27-30 bases long patch by POLD, POLE or POLK in TC-NER R-HSA-69091 Polymerase switching R-HSA-174411 Polymerase switching on the C-strand of the telomere R-HSA-174417 Telomere C-strand (Lagging Strand) Synthesis R-HSA-2564830 Cytosolic iron-sulfur cluster assembly R-HSA-69183 Processive synthesis on the lagging strand R-HSA-174437 Removal of the Flap Intermediate from the C-strand R-HSA-174414 Processive synthesis on the C-strand of the telomere R-HSA-5656169 Termination of translesion DNA synthesis R-HSA-110314 Recognition of DNA damage by PCNA-containing replication complex R-HSA-69109 Leading Strand Synthesis R-HSA-69186 Lagging Strand Synthesis R-HSA-180786 Extension of Telomeres R-HSA-5358565 Mismatch repair (MMR) directed by MSH2:MSH6 (MutSalpha) R-HSA-5358606 Mismatch repair (MMR) directed by MSH2:MSH3 (MutSbeta) R-HSA-1430728 Metabolism R-HSA-69166 Removal of the Flap Intermediate R-HSA-5651801 PCNA-Dependent Long Patch Base Excision Repair R-HSA-5696400 Dual Incision in GG-NER R-HSA-6782135 Dual incision in TC-NER R-HSA-110313 Translesion synthesis by Y family DNA polymerases bypasses lesions on DNA template R-HSA-73893 DNA Damage Bypass R-HSA-69190 DNA strand elongation R-HSA-157579 Telomere Maintenance R-HSA-5358508 Mismatch Repair R-HSA-110373 Resolution of AP sites via the multiple-nucleotide patch replacement pathway R-HSA-5696399 Global Genome Nucleotide Excision Repair (GG-NER) R-HSA-6781827 Transcription-Coupled Nucleotide Excision Repair (TC-NER) R-HSA-73894 DNA Repair R-HSA-69239 Synthesis of DNA R-HSA-73886 Chromosome Maintenance R-HSA-5685942 HDR through Homologous Recombination (HRR) R-HSA-73933 Resolution of Abasic Sites (AP sites) R-HSA-5696397 Gap-filling DNA repair synthesis and ligation in GG-NER R-HSA-6782210 Gap-filling DNA repair synthesis and ligation in TC-NER R-HSA-5696398 Nucleotide Excision Repair R-HSA-69242 S Phase R-HSA-69306 DNA Replication R-HSA-1640170 Cell Cycle R-HSA-5693567 HDR through Homologous Recombination (HR) or Single Strand Annealing (SSA) R-HSA-73884 Base Excision Repair R-HSA-69278 Cell Cycle (Mitotic) R-HSA-5693538 Homology Directed Repair R-HSA-5693532 DNA Double-Strand Break Repair
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Common Gene Haplotype Alleles 
