ID:DPOE3_HUMAN DESCRIPTION: RecName: Full=DNA polymerase epsilon subunit 3; EC=2.7.7.7; AltName: Full=Arsenic-transactivated protein; Short=AsTP; AltName: Full=Chromatin accessibility complex 17 kDa protein; Short=CHRAC-17; Short=HuCHRAC17; AltName: Full=DNA polymerase II subunit 3; AltName: Full=DNA polymerase epsilon subunit p17; FUNCTION: Forms a complex with DNA polymerase epsilon subunit CHRAC1 and binds naked DNA, which is then incorporated into chromatin, aided by the nucleosome-remodeling activity of ISWI/SNF2H and ACF1. CATALYTIC ACTIVITY: Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). SUBUNIT: Component of the epsilon DNA polymerase complex consisting of four subunits: POLE, POLE2, POLE3 and POLE4. Interaction with POLE4 is a prerequisite for further binding with POLE and POLE2. Interacts with CHRAC1. Together with CHRAC1, ACF1 and ISWI/SNF2H proteins, it forms the ISWI chromatin-remodeling complex, CHRAC. INTERACTION: Q9Q2G4:ORF (xeno); NbExp=5; IntAct=EBI-744901, EBI-6248094; Q9NR33:POLE4; NbExp=3; IntAct=EBI-744901, EBI-867034; SUBCELLULAR LOCATION: Nucleus (Potential). TISSUE SPECIFICITY: Expressed in all tissues tested, including, heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. WEB RESOURCE: Name=NIEHS-SNPs; URL="http://egp.gs.washington.edu/data/pole3/";
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
SCOP Domains: 48371 - ARM repeat 47113 - Histone-fold
ModBase Predicted Comparative 3D Structure on Q9NRF9
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
Biological Process: GO:0000082 G1/S transition of mitotic cell cycle GO:0006260 DNA replication GO:0043966 histone H3 acetylation GO:0071897 DNA biosynthetic process
AK074629 - Homo sapiens cDNA FLJ90148 fis, clone HEMBB1001959, weakly similar to CCAAT-BINDING TRANSCRIPTION FACTOR SUBUNIT A. AK096050 - Homo sapiens cDNA FLJ38731 fis, clone KIDNE2010739, moderately similar to Homo sapiens CHRAC17 (CHRAC17) mRNA. AF070640 - Homo sapiens clone 24781 mRNA sequence. BC003166 - Homo sapiens polymerase (DNA directed), epsilon 3 (p17 subunit), mRNA (cDNA clone MGC:4348 IMAGE:2822216), complete cds. BC004170 - Homo sapiens polymerase (DNA directed), epsilon 3 (p17 subunit), mRNA (cDNA clone MGC:2725 IMAGE:2822216), complete cds. AK092840 - Homo sapiens cDNA FLJ35521 fis, clone SPLEN2001278, highly similar to Homo sapiens CHRAC17 (CHRAC17) mRNA. AK074782 - Homo sapiens cDNA FLJ90301 fis, clone NT2RP2000610, weakly similar to CCAAT-BINDING TRANSCRIPTION FACTOR SUBUNIT A. AK074762 - Homo sapiens cDNA FLJ90281 fis, clone NT2RP1000468, weakly similar to CCAAT-BINDING TRANSCRIPTION FACTOR SUBUNIT A. JD296304 - Sequence 277328 from Patent EP1572962. JD550529 - Sequence 531553 from Patent EP1572962. JD353672 - Sequence 334696 from Patent EP1572962. JD070593 - Sequence 51617 from Patent EP1572962. JD406385 - Sequence 387409 from Patent EP1572962. JD425945 - Sequence 406969 from Patent EP1572962. JD255863 - Sequence 236887 from Patent EP1572962. JD454712 - Sequence 435736 from Patent EP1572962. JD292847 - Sequence 273871 from Patent EP1572962. JD562851 - Sequence 543875 from Patent EP1572962. JD551364 - Sequence 532388 from Patent EP1572962. JD148686 - Sequence 129710 from Patent EP1572962. JD202203 - Sequence 183227 from Patent EP1572962. JD160224 - Sequence 141248 from Patent EP1572962. JD247760 - Sequence 228784 from Patent EP1572962. JD404172 - Sequence 385196 from Patent EP1572962. JD525868 - Sequence 506892 from Patent EP1572962. JD399753 - Sequence 380777 from Patent EP1572962. JD424439 - Sequence 405463 from Patent EP1572962. JD536793 - Sequence 517817 from Patent EP1572962. JD255371 - Sequence 236395 from Patent EP1572962. JD392307 - Sequence 373331 from Patent EP1572962. JD214118 - Sequence 195142 from Patent EP1572962. JD254475 - Sequence 235499 from Patent EP1572962. JD123885 - Sequence 104909 from Patent EP1572962. JD021812 - Sequence 2836 from Patent EP1572962. JD027916 - Sequence 8940 from Patent EP1572962. JD469593 - Sequence 450617 from Patent EP1572962. JD073193 - Sequence 54217 from Patent EP1572962. JD381058 - Sequence 362082 from Patent EP1572962. JD316987 - Sequence 298011 from Patent EP1572962. JD313207 - Sequence 294231 from Patent EP1572962. JD453930 - Sequence 434954 from Patent EP1572962. JD083761 - Sequence 64785 from Patent EP1572962. AF226077 - Homo sapiens CHRAC17 (CHRAC17) mRNA, complete cds. AY720898 - Homo sapiens arsenic transactivated protein mRNA, complete cds. JF432738 - Synthetic construct Homo sapiens clone IMAGE:100073982 polymerase (DNA directed), epsilon 3 (p17 subunit) (POLE3) gene, encodes complete protein. KJ894010 - Synthetic construct Homo sapiens clone ccsbBroadEn_03404 POLE3 gene, encodes complete protein. AB527738 - Synthetic construct DNA, clone: pF1KB7911, Homo sapiens POLE3 gene for polymerase (DNA directed), epsilon 3, without stop codon, in Flexi system.
Biochemical and Signaling Pathways
Reactome (by CSHL, EBI, and GO)
Protein Q9NRF9 (Reactome details) participates in the following event(s):
R-HSA-68960 DNA polymerase epsilon binds at the origin R-HSA-68913 The primase component of DNA polymerase:primase synthesizes a 6-10 nucleotide RNA primer at the origin R-HSA-174425 The primase component of DNA polymerase:primase synthesizes a 6-10 nucleotide RNA primer on the G strand of the telomere R-HSA-68914 DNA polymerase alpha:primase binds at the origin R-HSA-5653838 POLD,POLE binds deISGylated PCNA after TLS R-HSA-5652009 RAD18:UBE2B or RBX1:CUL4:DDB1:DTL monoubiquitinates PCNA R-HSA-110364 PCNA:POLD,POLE:RPA:RFC and FEN1 bind APEX1 R-HSA-5651992 PCNA-containing replication complex binds damaged dsDNA R-HSA-5651809 LIG1, APEX1 and PCNA:POLD,POLE:RPA:RFC dissociate from repaired DNA R-HSA-5653840 POLD,POLE complete replication of damaged DNA after TLS R-HSA-5690213 DNA polymerases delta, epsilon or kappa bind the GG-NER site R-HSA-6782211 DNA polymerases delta, epsilon or kappa bind the TC-NER site R-HSA-110368 POLD,POLE-mediated DNA strand displacement synthesis R-HSA-110371 LIG1 binds APEX1 and PCNA at SSB R-HSA-110363 FEN1 bound to PCNA and APEX1 cleaves flap ssDNA R-HSA-5651805 LIG1 bound to APEX1 and PCNA ligates SSB R-HSA-5652005 RAD18:UBE2B or RBX1:CUL4:DDB1:DTL ubiquitin ligase complex binds PCNA:POLD,POLE:RPA:RFC associated with damaged dsDNA R-HSA-5693593 D-loop extension by DNA polymerases R-HSA-5690997 Ligation of newly synthesized repair patch to incised DNA in GG-NER R-HSA-6782227 Ligation of newly synthesized repair patch to incised DNA in TC-NER R-HSA-5690988 3'-incision of DNA by ERCC5 (XPG) in GG-NER R-HSA-5691001 Repair DNA synthesis of ~27-30 bases long patch by POLD, POLE or POLK in GG-NER R-HSA-6782224 3' incision by ERCC5 (XPG) in TC-NER R-HSA-6782208 Repair DNA synthesis of ~27-30 bases long patch by POLD, POLE or POLK in TC-NER R-HSA-68962 Activation of the pre-replicative complex R-HSA-68952 DNA replication initiation R-HSA-174430 Telomere C-strand synthesis initiation R-HSA-5656169 Termination of translesion DNA synthesis R-HSA-110314 Recognition of DNA damage by PCNA-containing replication complex R-HSA-69002 DNA Replication Pre-Initiation R-HSA-69206 G1/S Transition R-HSA-69239 Synthesis of DNA R-HSA-174417 Telomere C-strand (Lagging Strand) Synthesis R-HSA-5651801 PCNA-Dependent Long Patch Base Excision Repair R-HSA-5696400 Dual Incision in GG-NER R-HSA-6782135 Dual incision in TC-NER R-HSA-110313 Translesion synthesis by Y family DNA polymerases bypasses lesions on DNA template R-HSA-73893 DNA Damage Bypass R-HSA-68874 M/G1 Transition R-HSA-453279 Mitotic G1-G1/S phases R-HSA-69242 S Phase R-HSA-69306 DNA Replication R-HSA-180786 Extension of Telomeres R-HSA-110373 Resolution of AP sites via the multiple-nucleotide patch replacement pathway R-HSA-5696399 Global Genome Nucleotide Excision Repair (GG-NER) R-HSA-6781827 Transcription-Coupled Nucleotide Excision Repair (TC-NER) R-HSA-73894 DNA Repair R-HSA-69278 Cell Cycle (Mitotic) R-HSA-157579 Telomere Maintenance R-HSA-5685942 HDR through Homologous Recombination (HRR) R-HSA-73933 Resolution of Abasic Sites (AP sites) R-HSA-5696397 Gap-filling DNA repair synthesis and ligation in GG-NER R-HSA-6782210 Gap-filling DNA repair synthesis and ligation in TC-NER R-HSA-5696398 Nucleotide Excision Repair R-HSA-1640170 Cell Cycle R-HSA-73886 Chromosome Maintenance R-HSA-5693567 HDR through Homologous Recombination (HR) or Single Strand Annealing (SSA) R-HSA-73884 Base Excision Repair R-HSA-5693538 Homology Directed Repair R-HSA-5693532 DNA Double-Strand Break Repair
US Server
Sequence and Links to Tools and Databases 
Common Gene Haplotype Alleles 
