ID:RPC2_HUMAN DESCRIPTION: RecName: Full=DNA-directed RNA polymerase III subunit RPC2; Short=RNA polymerase III subunit C2; EC=2.7.7.6; AltName: Full=C128; AltName: Full=DNA-directed RNA polymerase III 127.6 kDa polypeptide; AltName: Full=DNA-directed RNA polymerase III subunit B; FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol III is composed of mobile elements and RPC2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the cleft (By similarity). Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway. CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). SUBUNIT: Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits (By similarity). SUBCELLULAR LOCATION: Nucleus (By similarity). DISEASE: Defects in POLR3B are the cause of leukodystrophy, hypomyelinating, type 8, with or without oligodontia and/or hypogonadotropic hypogonadism (HLD8) [MIM:614381]. An autosomal recessive neurodegenerative disorder characterized by early childhood onset of cerebellar ataxia and mild intellectual disabilities associated with diffuse hypomyelination apparent on brain MRI. Variable features include oligodontia and/or hypogonadotropic hypogonadism. SIMILARITY: Belongs to the RNA polymerase beta chain family. SEQUENCE CAUTION: Sequence=BAA91527.1; Type=Erroneous initiation; Note=Translation N-terminally extended; Sequence=BAA91581.1; Type=Erroneous initiation; Note=Translation N-terminally extended;
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
SCOP Domains: 64484 - beta and beta-prime subunits of DNA dependent RNA-polymerase
ModBase Predicted Comparative 3D Structure on Q9NW08
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
Biological Process: GO:0002376 immune system process GO:0006351 transcription, DNA-templated GO:0006383 transcription from RNA polymerase III promoter GO:0032728 positive regulation of interferon-beta production GO:0045087 innate immune response GO:0045089 positive regulation of innate immune response GO:0051607 defense response to virus
BX537447 - Homo sapiens mRNA; cDNA DKFZp686D10173 (from clone DKFZp686D10173). BC046238 - Homo sapiens polymerase (RNA) III (DNA directed) polypeptide B, mRNA (cDNA clone MGC:52435 IMAGE:4905794), complete cds. AK291635 - Homo sapiens cDNA FLJ78662 complete cds, highly similar to Homo sapiens polymerase (RNA) III (DNA directed) polypeptide B, mRNA. AY092084 - Homo sapiens RNA polymerase III subunit RPC2 mRNA, complete cds. AK122713 - Homo sapiens cDNA FLJ16205 fis, clone CTONG2019400, highly similar to DNA-directed RNA polymerase III subunit 127.6 kDa polypeptide (EC 2.7.7.6). AK092031 - Homo sapiens cDNA FLJ34712 fis, clone MESAN2003852, highly similar to DNA-directed RNA polymerase III subunit 127.6 kDa polypeptide (EC 2.7.7.6). AX747324 - Sequence 849 from Patent EP1308459. JD201842 - Sequence 182866 from Patent EP1572962. BC017501 - Homo sapiens polymerase (RNA) III (DNA directed) polypeptide B, mRNA (cDNA clone IMAGE:4853407), partial cds. AK090636 - Homo sapiens cDNA FLJ33317 fis, clone BNGH42007460, highly similar to DNA-directed RNA polymerase III subunit 127.6 kDa polypeptide (EC 2.7.7.6). AX746551 - Sequence 76 from Patent EP1308459. BC033724 - Homo sapiens polymerase (RNA) III (DNA directed) polypeptide B, mRNA (cDNA clone IMAGE:4998794), partial cds. CQ873758 - Sequence 177 from Patent WO2004076622. DD413595 - Regulation of Mammalian Cells. AK001250 - Homo sapiens cDNA FLJ10388 fis, clone NT2RM4000024, moderately similar to DNA-DIRECTED RNA POLYMERASE III 128 KD POLYPEPTIDE (EC 2.7.7.6). AK001161 - Homo sapiens cDNA FLJ10299 fis, clone NT2RM2000013, moderately similar to DNA-DIRECTED RNA POLYMERASE III 128 KD POLYPEPTIDE (EC 2.7.7.6). KJ818092 - Homo sapiens interphase nucleus and mitotic apparatus associated protein (INMAP) mRNA, complete cds. BX648712 - Homo sapiens mRNA; cDNA DKFZp686B10117 (from clone DKFZp686B10117). JD184486 - Sequence 165510 from Patent EP1572962. JD172063 - Sequence 153087 from Patent EP1572962. JD244542 - Sequence 225566 from Patent EP1572962.
Biochemical and Signaling Pathways
Reactome (by CSHL, EBI, and GO)
Protein Q9NW08 (Reactome details) participates in the following event(s):
R-HSA-76056 Recruitment of RNA polymerase III to TFIIIB:TFIIIC:TFIIIA:Type 1 Promoter Complex R-HSA-83803 Recruitment of RNA Polymerase III to TFIIIB:SNAPc:Type 3 Promoter Complex R-HSA-83805 Recruitment of RNA Polymerase III to the TFIIIB:TFIIIC: Type 2 Promoter Complex R-HSA-113454 RNA Polymerase III Termination and release of transcribed RNA R-HSA-76060 RNA Polymerase III Promoter Opening at Type 1 Promoters R-HSA-112150 RNA Polymerase III Promoter Opening at Type 2 Promoters R-HSA-112152 RNA Polymerase III Promoter Opening at Type 3 Promoters R-HSA-112055 RNA Polymerase III Abortive Initiation At Type 1 Open Promoters R-HSA-112153 RNA Polymerase III Simple Start Sequence Initiation At Type 1 Promoters R-HSA-112149 RNA Polymerase III Abortive Initiation At Type 2 Open Promoters R-HSA-112155 RNA Polymerase III Simple Start Sequence Initiation At Type 2 Promoters R-HSA-112054 RNA Polymerase III Abortive Initiation At Type 3 Open Promoters R-HSA-112156 RNA Polymerase III Simple Start Sequence Initiation At Type 3 Promoters R-HSA-113442 RNA Polymerase III Retractive RNase Activity at U-tract Pause Sites R-HSA-113446 Initiation of RNA Polymerase III Productive Transcription R-HSA-113449 RNA Polymerase III Transcriptional Pause at Terminator Sequence R-HSA-113451 Resumption of RNA Polymerase III Productive Transcription R-HSA-113705 RNA Polymerase III Productive Transcription R-HSA-76061 RNA Polymerase III Transcription Initiation From Type 1 Promoter R-HSA-76071 RNA Polymerase III Transcription Initiation From Type 3 Promoter R-HSA-76066 RNA Polymerase III Transcription Initiation From Type 2 Promoter R-HSA-73980 RNA Polymerase III Transcription Termination R-HSA-749476 RNA Polymerase III Abortive And Retractive Initiation R-HSA-73780 RNA Polymerase III Chain Elongation R-HSA-76046 RNA Polymerase III Transcription Initiation R-HSA-74158 RNA Polymerase III Transcription R-HSA-1834949 Cytosolic sensors of pathogen-associated DNA R-HSA-74160 Gene expression (Transcription) R-HSA-168249 Innate Immune System R-HSA-168256 Immune System
US Server
Sequence and Links to Tools and Databases 
Common Gene Haplotype Alleles 
