ID:RPC8_HUMAN DESCRIPTION: RecName: Full=DNA-directed RNA polymerase III subunit RPC8; Short=RNA polymerase III subunit C8; AltName: Full=DNA-directed RNA polymerase III subunit H; AltName: Full=RNA polymerase III subunit 22.9 kDa subunit; Short=RPC22.9; FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway (By similarity). SUBUNIT: Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits (By similarity). Interacts with CRCP/RPC9. POLR3H/RPC8 and CRCP/RPC9 probably form a Pol III subcomplex. SUBCELLULAR LOCATION: Nucleus. SIMILARITY: Belongs to the eukaryotic RPB7/RPC8 RNA polymerase subunit family. SEQUENCE CAUTION: Sequence=BAB33335.1; Type=Erroneous initiation;
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on Q9Y535
Front
Top
Side
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.
Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
Gene Ontology (GO) Annotations with Structured Vocabulary
Molecular Function: GO:0003677 DNA binding GO:0003899 DNA-directed 5'-3' RNA polymerase activity GO:0001056 RNA polymerase III activity
Biological Process: GO:0002376 immune system process GO:0006139 nucleobase-containing compound metabolic process GO:0006351 transcription, DNA-templated GO:0006383 transcription from RNA polymerase III promoter GO:0006384 transcription initiation from RNA polymerase III promoter GO:0045087 innate immune response GO:0051607 defense response to virus
AK026524 - Homo sapiens cDNA: FLJ22871 fis, clone KAT02533. AK000365 - Homo sapiens cDNA FLJ20358 fis, clone HEP16618. AB051452 - Homo sapiens KIAA1665 mRNA for KIAA1665 protein. JD356127 - Sequence 337151 from Patent EP1572962. DQ596237 - Homo sapiens piRNA piR-34303, complete sequence. DQ599980 - Homo sapiens piRNA piR-38046, complete sequence. BC088367 - Homo sapiens polymerase (RNA) III (DNA directed) polypeptide H (22.9kD), mRNA (cDNA clone MGC:111097 IMAGE:30385483), complete cds. BC017248 - Homo sapiens polymerase (RNA) III (DNA directed) polypeptide H (22.9kD), mRNA (cDNA clone MGC:29654 IMAGE:4892259), complete cds. AY092087 - Homo sapiens RNA polymerase III subunit RPC8 mRNA, complete cds. JD125052 - Sequence 106076 from Patent EP1572962. JD372232 - Sequence 353256 from Patent EP1572962. JD473270 - Sequence 454294 from Patent EP1572962. JD421647 - Sequence 402671 from Patent EP1572962. CR456459 - Homo sapiens dJ347H13.5 full length open reading frame (ORF) cDNA clone (cDNA clone C22ORF:pGEM.dJ347H13.5). JD383893 - Sequence 364917 from Patent EP1572962. JD439767 - Sequence 420791 from Patent EP1572962. JD051760 - Sequence 32784 from Patent EP1572962. JD465770 - Sequence 446794 from Patent EP1572962. JD496509 - Sequence 477533 from Patent EP1572962. CU013009 - Homo sapiens POLR3H, mRNA (cDNA clone IMAGE:100000172), complete cds, with stop codon, in Gateway system. AB384256 - Synthetic construct DNA, clone: pF1KSDA1665, Homo sapiens POLR3H gene for DNA-directed RNA polymerase III subunit RPC8, complete cds, without stop codon, in Flexi system. GQ129271 - Synthetic construct Homo sapiens clone HAIB:100068667; DKFZo004C0536 polymerase (RNA) III (DNA directed) polypeptide H (22.9kD) protein (POLR3H) gene, partial cds. GQ129272 - Synthetic construct Homo sapiens clone HAIB:100068570; DKFZo008C0535 polymerase (RNA) III (DNA directed) polypeptide H (22.9kD) protein (POLR3H) gene, complete cds. CU013297 - Homo sapiens POLR3H, mRNA (cDNA clone IMAGE:100000076), complete cds, without stop codon, in Gateway system. AK129663 - Homo sapiens cDNA FLJ26152 fis, clone ADG00585. JD497598 - Sequence 478622 from Patent EP1572962. JD497600 - Sequence 478624 from Patent EP1572962. JD497599 - Sequence 478623 from Patent EP1572962.
Biochemical and Signaling Pathways
Reactome (by CSHL, EBI, and GO)
Protein Q9Y535 (Reactome details) participates in the following event(s):
R-HSA-76056 Recruitment of RNA polymerase III to TFIIIB:TFIIIC:TFIIIA:Type 1 Promoter Complex R-HSA-83803 Recruitment of RNA Polymerase III to TFIIIB:SNAPc:Type 3 Promoter Complex R-HSA-83805 Recruitment of RNA Polymerase III to the TFIIIB:TFIIIC: Type 2 Promoter Complex R-HSA-113454 RNA Polymerase III Termination and release of transcribed RNA R-HSA-76060 RNA Polymerase III Promoter Opening at Type 1 Promoters R-HSA-112150 RNA Polymerase III Promoter Opening at Type 2 Promoters R-HSA-112152 RNA Polymerase III Promoter Opening at Type 3 Promoters R-HSA-112055 RNA Polymerase III Abortive Initiation At Type 1 Open Promoters R-HSA-112153 RNA Polymerase III Simple Start Sequence Initiation At Type 1 Promoters R-HSA-112149 RNA Polymerase III Abortive Initiation At Type 2 Open Promoters R-HSA-112155 RNA Polymerase III Simple Start Sequence Initiation At Type 2 Promoters R-HSA-112054 RNA Polymerase III Abortive Initiation At Type 3 Open Promoters R-HSA-112156 RNA Polymerase III Simple Start Sequence Initiation At Type 3 Promoters R-HSA-113442 RNA Polymerase III Retractive RNase Activity at U-tract Pause Sites R-HSA-113446 Initiation of RNA Polymerase III Productive Transcription R-HSA-113449 RNA Polymerase III Transcriptional Pause at Terminator Sequence R-HSA-113451 Resumption of RNA Polymerase III Productive Transcription R-HSA-113705 RNA Polymerase III Productive Transcription R-HSA-76061 RNA Polymerase III Transcription Initiation From Type 1 Promoter R-HSA-76071 RNA Polymerase III Transcription Initiation From Type 3 Promoter R-HSA-76066 RNA Polymerase III Transcription Initiation From Type 2 Promoter R-HSA-73980 RNA Polymerase III Transcription Termination R-HSA-749476 RNA Polymerase III Abortive And Retractive Initiation R-HSA-73780 RNA Polymerase III Chain Elongation R-HSA-76046 RNA Polymerase III Transcription Initiation R-HSA-74158 RNA Polymerase III Transcription R-HSA-1834949 Cytosolic sensors of pathogen-associated DNA R-HSA-74160 Gene expression (Transcription) R-HSA-168249 Innate Immune System R-HSA-168256 Immune System
US Server
Sequence and Links to Tools and Databases 
Common Gene Haplotype Alleles 
