ID:2AAA_HUMAN DESCRIPTION: RecName: Full=Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform; AltName: Full=Medium tumor antigen-associated 61 kDa protein; AltName: Full=PP2A subunit A isoform PR65-alpha; AltName: Full=PP2A subunit A isoform R1-alpha; FUNCTION: The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. Required for proper chromosome segregation and for centromeric localization of SGOL1 in mitosis. SUBUNIT: Found in a complex with at least ARL2, PPP2CB, PPP2R1A, PPP2R2A, PPP2R5E and TBCD. Interacts with FOXO1; the interaction dephosphorylates FOXO1 on AKT-mediated phosphoylation sites (By similarity). PP2A consists of a common heterodimeric core enzyme, composed of PPP2CA a 36 kDa catalytic subunit (subunit C) and PPP2R1A a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules. Interacts with IPO9. Interacts with TP53 and SGOL1. Interacts with PLA2G16; this interaction might decrease PP2A activity. INTERACTION: Q8TCG1:KIAA1524; NbExp=4; IntAct=EBI-302388, EBI-1379376; P53816:PLA2G16; NbExp=7; IntAct=EBI-302388, EBI-746318; P67775:PPP2CA; NbExp=14; IntAct=EBI-302388, EBI-712311; Q00005:PPP2R2B; NbExp=5; IntAct=EBI-302388, EBI-1052159; Q13362-1:PPP2R5C; NbExp=3; IntAct=EBI-302388, EBI-1266170; Q13362-2:PPP2R5C; NbExp=2; IntAct=EBI-302388, EBI-1266173; Q60996-3:Ppp2r5c (xeno); NbExp=2; IntAct=EBI-302388, EBI-1369292; P60510:PPP4C; NbExp=3; IntAct=EBI-302388, EBI-1046072; O43815:STRN; NbExp=4; IntAct=EBI-302388, EBI-1046642; SUBCELLULAR LOCATION: Cytoplasm (By similarity). Chromosome, centromere. Note=Centromeric localization requires the presence of BUB1. DOMAIN: Each HEAT repeat appears to consist of two alpha helices joined by a hydrophilic region, the intrarepeat loop. The repeat units may be arranged laterally to form a rod-like structure. SIMILARITY: Belongs to the phosphatase 2A regulatory subunit A family. SIMILARITY: Contains 15 HEAT repeats.
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on P30153
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
Gene Ontology (GO) Annotations with Structured Vocabulary
Molecular Function: GO:0004722 protein serine/threonine phosphatase activity GO:0005515 protein binding GO:0019888 protein phosphatase regulator activity GO:0046982 protein heterodimerization activity GO:1990405 protein antigen binding
Biological Process: GO:0000086 G2/M transition of mitotic cell cycle GO:0000184 nuclear-transcribed mRNA catabolic process, nonsense-mediated decay GO:0000188 inactivation of MAPK activity GO:0006275 regulation of DNA replication GO:0006355 regulation of transcription, DNA-templated GO:0006470 protein dephosphorylation GO:0006672 ceramide metabolic process GO:0006915 apoptotic process GO:0007059 chromosome segregation GO:0007143 female meiotic division GO:0008380 RNA splicing GO:0010033 response to organic substance GO:0010389 regulation of G2/M transition of mitotic cell cycle GO:0019932 second-messenger-mediated signaling GO:0030111 regulation of Wnt signaling pathway GO:0030155 regulation of cell adhesion GO:0030308 negative regulation of cell growth GO:0040008 regulation of growth GO:0042532 negative regulation of tyrosine phosphorylation of STAT protein GO:0043666 regulation of phosphoprotein phosphatase activity GO:0045595 regulation of cell differentiation GO:0051232 meiotic spindle elongation GO:0051306 mitotic sister chromatid separation GO:0051754 meiotic sister chromatid cohesion, centromeric GO:0065003 macromolecular complex assembly GO:0070262 peptidyl-serine dephosphorylation GO:0097711 ciliary basal body docking GO:1903538 regulation of meiotic cell cycle process involved in oocyte maturation GO:2001241 positive regulation of extrinsic apoptotic signaling pathway in absence of ligand
HM005541 - Homo sapiens clone HTL-S-1 testicular secretory protein Li 1 mRNA, complete cds. AK315700 - Homo sapiens cDNA, FLJ96799, Homo sapiens protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), alpha isoform (PPP2R1A), mRNA. AK290593 - Homo sapiens cDNA FLJ77680 complete cds, highly similar to Homo sapiens protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), alpha isoform (PPP2R1A), mRNA. AK291932 - Homo sapiens cDNA FLJ78455 complete cds, highly similar to Homo sapiens protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), alpha isoform (PPP2R1A), mRNA. AK293174 - Homo sapiens cDNA FLJ53296 complete cds, highly similar to Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform. AK130000 - Homo sapiens cDNA FLJ26490 fis, clone KDN05953, highly similar to Serine/threonine protein phosphatase 2A, 65 kDa regulatory subunit A, alpha isoform. AK091387 - Homo sapiens cDNA FLJ34068 fis, clone FCBBF3001918, highly similar to SERINE/THREONINE PROTEIN PHOSPHATASE 2A, 65 KDA REGULATORY SUBUNIT A, ALPHA ISOFORM. AX746952 - Sequence 477 from Patent EP1308459. BC026733 - Homo sapiens protein phosphatase 2 (formerly 2A), regulatory subunit A, alpha isoform, mRNA (cDNA clone IMAGE:5111467). AK299012 - Homo sapiens cDNA FLJ56133 complete cds, highly similar to Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform. AK303934 - Homo sapiens cDNA FLJ59603 complete cds, highly similar to Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform. AK307743 - Homo sapiens cDNA, FLJ97691. J02902 - Human protein phosphatase 2A regulatory subunit alpha-isotype (alpha-PR65) mRNA, complete cds. BC001537 - Homo sapiens protein phosphatase 2 (formerly 2A), regulatory subunit A, alpha isoform, mRNA (cDNA clone MGC:786 IMAGE:2987938), complete cds. AK293493 - Homo sapiens cDNA FLJ56053 complete cds, highly similar to Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform. JD342994 - Sequence 324018 from Patent EP1572962. KJ891869 - Synthetic construct Homo sapiens clone ccsbBroadEn_01263 PPP2R1A gene, encodes complete protein. AB384756 - Synthetic construct DNA, clone: pF1KB3191, Homo sapiens PPP2R1A gene for serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform, complete cds, without stop codon, in Flexi system. CR450340 - Homo sapiens full open reading frame cDNA clone RZPDo834D1214D for gene PPP2R1A, protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), alpha isoform; complete cds; without stopcodon. DQ892756 - Synthetic construct clone IMAGE:100005386; FLH189394.01X; RZPDo839E0974D protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), alpha isoform (PPP2R1A) gene, encodes complete protein. DQ896000 - Synthetic construct Homo sapiens clone IMAGE:100010460; FLH189390.01L; RZPDo839E0964D protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), alpha isoform (PPP2R1A) gene, encodes complete protein. AK090488 - Homo sapiens cDNA FLJ33169 fis, clone ADRGL2000384, highly similar to Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform. JD025370 - Sequence 6394 from Patent EP1572962. JD029501 - Sequence 10525 from Patent EP1572962. JD133256 - Sequence 114280 from Patent EP1572962. JD565616 - Sequence 546640 from Patent EP1572962. JD366904 - Sequence 347928 from Patent EP1572962. JD439556 - Sequence 420580 from Patent EP1572962. JD452399 - Sequence 433423 from Patent EP1572962. JD254266 - Sequence 235290 from Patent EP1572962. JD132663 - Sequence 113687 from Patent EP1572962. JD253059 - Sequence 234083 from Patent EP1572962. JD123708 - Sequence 104732 from Patent EP1572962. JD323610 - Sequence 304634 from Patent EP1572962. JD128676 - Sequence 109700 from Patent EP1572962. JD468111 - Sequence 449135 from Patent EP1572962. JD390519 - Sequence 371543 from Patent EP1572962.
Biochemical and Signaling Pathways
Reactome (by CSHL, EBI, and GO)
Protein P30153 (Reactome details) participates in the following event(s):
R-HSA-380272 Plk1-mediated phosphorylation of Nlp R-HSA-380283 Recruitment of additional gamma tubulin/ gamma TuRC to the centrosome R-HSA-380294 Loss of C-Nap-1 from centrosomes R-HSA-380311 Recruitment of Plk1 to centrosomes R-HSA-380455 Recruitment of CDK11p58 to the centrosomes R-HSA-380303 Dissociation of Phospho-Nlp from the centrosome R-HSA-1295622 SPRY2 binds CBL R-HSA-5626220 C2CD3 binds the mother centriole R-HSA-8856945 PP2A methylation by LCMT1 R-HSA-8856951 PP2A demethylation by PPME1 R-HSA-2430552 p-S62-ARPP19/p-S67-ENSA binds PP2A-PPP2R2D R-HSA-6792871 CCNG1 recruits PP2A-PPP2R5C to MDM2 R-HSA-6792863 PP2A-PP2R5C dephosphorylates MDM2 R-HSA-2995388 PP2A dephosphorylates BANF1 R-HSA-380508 Translocation of NuMA to the centrosomes R-HSA-2574845 AJUBA binds centrosome-associated AURKA R-HSA-8853405 TPX2 binds AURKA at centrosomes R-HSA-3000319 BORA binds PLK1 and AURKA R-HSA-2574840 AJUBA facilitates AURKA autophosphorylation R-HSA-3000310 AURKA phosphorylates PLK1 R-HSA-934559 SPRY2 is phosphorylated by phosphorylated MNK1 R-HSA-1295609 SRC phosphorylates SPRY2 on Y55 and Y227 R-HSA-1295599 SPRY2 translocates to the plasma membrane R-HSA-1295613 SPRY2 binds GRB2 R-HSA-1549564 PPTN11 dephosphorylates SPRY2 R-HSA-1295632 PPA2A dephosphorylates SPRY2 R-HSA-5626223 C2CD3 and OFD1 recruit 5 distal appendage proteins to the centriole R-HSA-5626681 Recruitment of transition zone proteins R-HSA-5626227 CP110 and CEP97 dissociate from the centriole R-HSA-163769 Activation of PP2A by Xylulose-5-phosphate R-HSA-195251 Assembly of the destruction complex R-HSA-389532 PP2A binds CTLA4 homodimer R-HSA-432143 PECAM-1 binds PP2A R-HSA-8857925 Inhibition of PP2A activity by phosphorylation of the catalytic subunit at tyrosine Y307 R-HSA-201685 Beta-catenin is released from the destruction complex R-HSA-388833 Phosphorylation of CTLA-4 R-HSA-6811454 MAPKs phosphorylate PP2A R-HSA-6811472 IER3 recruits MAPKs to PP2A-B56-beta,gamma R-HSA-163688 Dephosphorylation of pChREBP (Thr 666) by PP2A R-HSA-164056 Dephosphorylation of pChREBP (Ser 568) by PP2A R-HSA-199959 ERKs are inactivated by protein phosphatase 2A R-HSA-113503 PP2A mediated localization of RB1 protein in chromatin R-HSA-1363274 Dephosphorylation of p107 (RBL1) by PP2A R-HSA-1363276 Dephosphorylation of p130 (RBL2) by PP2A R-HSA-4088141 PP2A-PPP2R2A dephosphorylates FOXM1 R-HSA-380316 Association of NuMA with microtubules R-HSA-8853419 TPX2 promotes AURKA autophosphorylation R-HSA-5626228 The distal appendage proteins recruit TTBK2 R-HSA-5638009 CEP164 recruits RAB3IP-carrying Golgi-derived vesicles to the basal body R-HSA-163689 Dephosphorylation of pChREBP (Ser 196) by PP2A R-HSA-163750 Dephosphorylation of phosphoPFKFB1 by PP2A complex R-HSA-201790 DARPP-32 is dephosphorylated on Thr75 by PP2A R-HSA-5229343 AXIN is phosphorylated in the destruction complex R-HSA-195280 Dissociation of beta-catenin from Axin and association of beta catenin with phospho-(20 aa) APC in the detruction complex R-HSA-195275 Phosphorylation of APC component of the destruction complex R-HSA-2130279 Association of beta-catenin with the RBX1:SCF(beta-TrCP1) ubiquitin ligase complex R-HSA-141409 Mad1 binds kinetochore R-HSA-375302 Kinetochore capture of astral microtubules R-HSA-5666129 CDC42:GTP recruits DIAPH2-2 to kinetochores R-HSA-5666169 Kinetochore capture of astral microtubules is positively regulated by CDC42:GTP:p-S196-DIAPH2-2 R-HSA-388809 CTLA-4 binds B7-1/B7-2 R-HSA-195304 Association of beta-catenin with the destruction complex R-HSA-390329 Dephosphorylation of AKT by PP2A R-HSA-5672957 PP2A dephosphorylates KSR1 R-HSA-5672961 PP2A dephosphorylates inactive RAFs R-HSA-5675431 PP2A dephosphorylates RAF1 R-HSA-6811504 AKT1 dephosphorylation by PP2A-B56-beta,gamma R-HSA-5626699 MARK4 binds ODF2 in the centriole R-HSA-2130286 Multi-ubiquitination of phospho-beta-catenin by RBX1:SCF(beta-TrCP1) R-HSA-141431 MAD2 associates with the Mad1 kinetochore complex R-HSA-141439 Release of activated MAD2 from kinetochores R-HSA-2467811 Separation of sister chromatids R-HSA-2467809 ESPL1 (Separase) cleaves centromeric cohesin R-HSA-5666160 AURKB phosphorylates DIAPH2-2 at kinetochores R-HSA-195287 Phosphorylation of phospho-(Ser45 ) at Thr 41 by GSK-3 R-HSA-195318 Phosphorylation of beta-catenin at Ser45 by CK1 alpha R-HSA-195300 Phosphorylation of phospho-(Ser45,Thr41,Ser37) at Ser33 by GSK-3 R-HSA-195283 Phosphorylation of phospho- (Ser45, Thr41) beta-catenin at Ser37 by GSK-3 R-HSA-5617816 RAB3IP stimulates nucleotide exchange on RAB8A R-HSA-141422 MAD2 converted to an inhibitory state via interaction with Mad1 R-HSA-1638821 PP2A-B56 dephosphorylates centromeric cohesin R-HSA-1638803 Phosphorylation of cohesin by PLK1 at centromeres R-HSA-2468287 CDK1 phosphorylates CDCA5 (Sororin) at centromeres R-HSA-975957 Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) R-HSA-380259 Loss of Nlp from mitotic centrosomes R-HSA-380270 Recruitment of mitotic centrosome proteins and complexes R-HSA-380284 Loss of proteins required for interphase microtubule organization from the centrosome R-HSA-1295596 Spry regulation of FGF signaling R-HSA-5620912 Anchoring of the basal body to the plasma membrane R-HSA-69273 Cyclin A/B1/B2 associated events during G2/M transition R-HSA-2465910 MASTL Facilitates Mitotic Progression R-HSA-6804757 Regulation of TP53 Degradation R-HSA-927802 Nonsense-Mediated Decay (NMD) R-HSA-2995383 Initiation of Nuclear Envelope Reformation R-HSA-380320 Recruitment of NuMA to mitotic centrosomes R-HSA-2565942 Regulation of PLK1 Activity at G2/M Transition R-HSA-8854518 AURKA Activation by TPX2 R-HSA-380287 Centrosome maturation R-HSA-5654726 Negative regulation of FGFR1 signaling R-HSA-5654727 Negative regulation of FGFR2 signaling R-HSA-5654732 Negative regulation of FGFR3 signaling R-HSA-5654733 Negative regulation of FGFR4 signaling R-HSA-5617833 Cilium Assembly R-HSA-163685 Energy Metabolism R-HSA-195253 Degradation of beta-catenin by the destruction complex R-HSA-389513 CTLA4 inhibitory signaling R-HSA-432142 Platelet sensitization by LDL R-HSA-5467337 APC truncation mutants have impaired AXIN binding R-HSA-5467340 AXIN missense mutants destabilize the destruction complex R-HSA-5467348 Truncations of AMER1 destabilize the destruction complex R-HSA-6811558 PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling R-HSA-4641262 Disassembly of the destruction complex and recruitment of AXIN to the membrane R-HSA-163767 PP2A-mediated dephosphorylation of key metabolic factors R-HSA-198753 ERK/MAPK targets R-HSA-202670 ERKs are inactivated R-HSA-113501 Inhibition of replication initiation of damaged DNA by RB1/E2F1 R-HSA-69275 G2/M Transition R-HSA-69231 Cyclin D associated events in G1 R-HSA-68875 Mitotic Prophase R-HSA-6806003 Regulation of TP53 Expression and Degradation R-HSA-8953854 Metabolism of RNA R-HSA-2995410 Nuclear Envelope Reassembly R-HSA-68877 Mitotic Prometaphase R-HSA-5654736 Signaling by FGFR1 R-HSA-5654738 Signaling by FGFR2 R-HSA-5654741 Signaling by FGFR3 R-HSA-5654743 Signaling by FGFR4 R-HSA-1852241 Organelle biogenesis and maintenance R-HSA-1430728 Metabolism R-HSA-70171 Glycolysis R-HSA-180024 DARPP-32 events R-HSA-141444 Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal R-HSA-5663220 RHO GTPases Activate Formins R-HSA-195721 Signaling by WNT R-HSA-388841 Costimulation by the CD28 family R-HSA-418346 Platelet homeostasis R-HSA-4839744 truncated APC mutants destabilize the destruction complex R-HSA-4839735 AXIN mutants destabilize the destruction complex, activating WNT signaling R-HSA-4839748 AMER1 mutants destabilize the destruction complex R-HSA-199418 Negative regulation of the PI3K/AKT network R-HSA-201681 TCF dependent signaling in response to WNT R-HSA-5673000 RAF activation R-HSA-5675221 Negative regulation of MAPK pathway R-HSA-198725 Nuclear Events (kinase and transcription factor activation) R-HSA-450282 MAPK targets/ Nuclear events mediated by MAP kinases R-HSA-113510 E2F mediated regulation of DNA replication R-HSA-453274 Mitotic G2-G2/M phases R-HSA-69236 G1 Phase R-HSA-68886 M Phase R-HSA-5633007 Regulation of TP53 Activity R-HSA-68882 Mitotic Anaphase R-HSA-190236 Signaling by FGFR R-HSA-70326 Glucose metabolism R-HSA-111885 Opioid Signalling R-HSA-5358751 S45 mutants of beta-catenin aren't phosphorylated R-HSA-5358752 T41 mutants of beta-catenin aren't phosphorylated R-HSA-5358749 S37 mutants of beta-catenin aren't phosphorylated R-HSA-5358747 S33 mutants of beta-catenin aren't phosphorylated R-HSA-2500257 Resolution of Sister Chromatid Cohesion R-HSA-2467813 Separation of Sister Chromatids R-HSA-141424 Amplification of signal from the kinetochores R-HSA-195258 RHO GTPase Effectors R-HSA-196299 Beta-catenin phosphorylation cascade R-HSA-5339716 Misspliced GSK3beta mutants stabilize beta-catenin R-HSA-162582 Signal Transduction R-HSA-1280218 Adaptive Immune System R-HSA-109582 Hemostasis R-HSA-4791275 Signaling by WNT in cancer R-HSA-1257604 PIP3 activates AKT signaling R-HSA-5673001 RAF/MAP kinase cascade R-HSA-187037 Signaling by NTRK1 (TRKA) R-HSA-450294 MAP kinase activation R-HSA-69206 G1/S Transition R-HSA-69278 Cell Cycle (Mitotic) R-HSA-453279 Mitotic G1-G1/S phases R-HSA-3700989 Transcriptional Regulation by TP53 R-HSA-2555396 Mitotic Metaphase and Anaphase R-HSA-9006934 Signaling by Receptor Tyrosine Kinases R-HSA-71387 Metabolism of carbohydrates R-HSA-418594 G alpha (i) signalling events R-HSA-4839743 phosphorylation site mutants of CTNNB1 are not targeted to the proteasome by the destruction complex R-HSA-69618 Mitotic Spindle Checkpoint R-HSA-194315 Signaling by Rho GTPases R-HSA-168256 Immune System R-HSA-5663202 Diseases of signal transduction R-HSA-9006925 Intracellular signaling by second messengers R-HSA-5684996 MAPK1/MAPK3 signaling R-HSA-166520 Signaling by NTRKs R-HSA-166058 MyD88:Mal cascade initiated on plasma membrane R-HSA-168164 Toll Like Receptor 3 (TLR3) Cascade R-HSA-448424 Interleukin-17 signaling R-HSA-937061 TRIF(TICAM1)-mediated TLR4 signaling R-HSA-975138 TRAF6 mediated induction of NFkB and MAP kinases upon TLR7/8 or 9 activation R-HSA-975871 MyD88 cascade initiated on plasma membrane R-HSA-1640170 Cell Cycle R-HSA-212436 Generic Transcription Pathway R-HSA-388396 GPCR downstream signalling R-HSA-69620 Cell Cycle Checkpoints R-HSA-1643685 Disease R-HSA-5683057 MAPK family signaling cascades R-HSA-166016 Toll Like Receptor 4 (TLR4) Cascade R-HSA-168179 Toll Like Receptor TLR1:TLR2 Cascade R-HSA-168188 Toll Like Receptor TLR6:TLR2 Cascade R-HSA-168898 Toll-Like Receptors Cascades R-HSA-449147 Signaling by Interleukins R-HSA-166166 MyD88-independent TLR4 cascade R-HSA-975155 MyD88 dependent cascade initiated on endosome R-HSA-168142 Toll Like Receptor 10 (TLR10) Cascade R-HSA-168176 Toll Like Receptor 5 (TLR5) Cascade R-HSA-73857 RNA Polymerase II Transcription R-HSA-372790 Signaling by GPCR R-HSA-181438 Toll Like Receptor 2 (TLR2) Cascade R-HSA-168249 Innate Immune System R-HSA-1280215 Cytokine Signaling in Immune system R-HSA-168138 Toll Like Receptor 9 (TLR9) Cascade R-HSA-168181 Toll Like Receptor 7/8 (TLR7/8) Cascade R-HSA-74160 Gene expression (Transcription)
US Server
Sequence and Links to Tools and Databases 
Common Gene Haplotype Alleles 
