Human Gene PRKAB2 (ENST00000254101.4_7) from GENCODE V47lift37
  Description: protein kinase AMP-activated non-catalytic subunit beta 2, transcript variant 2 (from RefSeq NR_103871.2)
Gencode Transcript: ENST00000254101.4_7
Gencode Gene: ENSG00000131791.8_9
Transcript (Including UTRs)
   Position: hg19 chr1:146,626,685-146,644,049 Size: 17,365 Total Exon Count: 8 Strand: -
Coding Region
   Position: hg19 chr1:146,631,144-146,643,723 Size: 12,580 Coding Exon Count: 7 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr1:146,626,685-146,644,049)mRNA (may differ from genome)Protein (272 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMGIOMIMPubMedReactomeUniProtKB
WikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: AAKB2_HUMAN
DESCRIPTION: RecName: Full=5'-AMP-activated protein kinase subunit beta-2; Short=AMPK subunit beta-2;
FUNCTION: Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3).
SUBUNIT: AMPK is a heterotrimer of an alpha catalytic subunit (PRKAA1 or PRKAA2), a beta (PRKAB1 or PRKAB2) and a gamma non- catalytic subunits (PRKAG1, PRKAG2 or PRKAG3).
INTERACTION: Self; NbExp=2; IntAct=EBI-1053424, EBI-1053424; Q13131:PRKAA1; NbExp=5; IntAct=EBI-1053424, EBI-1181405; P54619:PRKAG1; NbExp=3; IntAct=EBI-1053424, EBI-1181439;
PTM: Phosphorylated when associated with the catalytic subunit (PRKAA1 or PRKAA2). Phosphorylated by ULK1 and ULK2; leading to negatively regulate AMPK activity and suggesting the existence of a regulatory feedback loop between ULK1, ULK2 and AMPK.
SIMILARITY: Belongs to the 5'-AMP-activated protein kinase beta subunit family.

-  Primer design for this transcript
 

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Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 30.99 RPKM in Muscle - Skeletal
Total median expression: 532.10 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -19.3065-0.297 Picture PostScript Text
3' UTR -1291.604459-0.290 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR006828 - AMP_prot_kin_bsu_interact-dom

Pfam Domains:
PF04739 - 5'-AMP-activated protein kinase beta subunit, interaction domain
PF16561 - Glycogen recognition site of AMP-activated protein kinase

SCOP Domains:
81296 - E set domains
75011 - 3-carboxy-cis,cis-mucoante lactonizing enzyme
101908 - Putative isomerase YbhE
160219 - AMPKBI-like

Protein Data Bank (PDB) 3-D Structure
MuPIT help
2F15 - X-ray MuPIT 2V8Q - X-ray MuPIT 2V92 - X-ray MuPIT 2V9J - X-ray MuPIT 2Y8L - X-ray MuPIT 2Y8Q - X-ray MuPIT 2Y94 - X-ray 2YA3 - X-ray MuPIT 4EAI - X-ray MuPIT 4EAJ - X-ray MuPIT


ModBase Predicted Comparative 3D Structure on O43741
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologGenome BrowserGenome Browser
Gene DetailsGene Details  Gene DetailsGene Details
Gene SorterGene Sorter  Gene SorterGene Sorter
 RGD  WormBaseSGD
    Protein SequenceProtein Sequence
    AlignmentAlignment

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0005515 protein binding
GO:0042802 identical protein binding
GO:0004679 AMP-activated protein kinase activity

Biological Process:
GO:0006468 protein phosphorylation
GO:0006629 lipid metabolic process
GO:0006631 fatty acid metabolic process
GO:0006633 fatty acid biosynthetic process
GO:0007050 cell cycle arrest
GO:0007165 signal transduction
GO:0016236 macroautophagy
GO:0042304 regulation of fatty acid biosynthetic process

Cellular Component:
GO:0005654 nucleoplasm
GO:0005829 cytosol
GO:0031588 nucleotide-activated protein kinase complex


-  Descriptions from all associated GenBank mRNAs
  BC053610 - Homo sapiens protein kinase, AMP-activated, beta 2 non-catalytic subunit, mRNA (cDNA clone MGC:61468 IMAGE:6091476), complete cds.
AK023717 - Homo sapiens cDNA FLJ13655 fis, clone PLACE1011503.
DQ594355 - Homo sapiens piRNA piR-60467, complete sequence.
AK304069 - Homo sapiens cDNA FLJ52049 complete cds, weakly similar to 5'-AMP-activated protein kinase subunit beta-2.
AK292820 - Homo sapiens cDNA FLJ76470 complete cds, highly similar to Homo sapiens protein kinase, AMP-activated, beta 2 non-catalytic subunit (PRKAB2), mRNA.
AK316183 - Homo sapiens cDNA, FLJ79082 complete cds, weakly similar to 5'-AMP-activated protein kinase subunit beta-2.
AK316005 - Homo sapiens cDNA, FLJ78904 complete cds, highly similar to 5'-AMP-activated protein kinase subunit beta-2.
AK294863 - Homo sapiens cDNA FLJ50408 complete cds, highly similar to 5'-AMP-activated protein kinase subunit beta-2.
AJ224538 - Homo sapiens mRNA for AMP-activated protein kinase beta 2 subunit.
HQ257946 - Synthetic construct Homo sapiens clone IMAGE:100072255 protein kinase, AMP-activated, beta 2 non-catalytic subunit (PRKAB2) gene, encodes complete protein.
KJ891883 - Synthetic construct Homo sapiens clone ccsbBroadEn_01277 PRKAB2 gene, encodes complete protein.
KJ905280 - Synthetic construct Homo sapiens clone ccsbBroadEn_14779 PRKAB2 gene, encodes complete protein.
KJ534919 - Homo sapiens clone PRKAB2_iso-B_fetal-F01 AMP-activated protein kinase beta 2 non-catalytic subunit isoform B (PRKAB2) mRNA, partial cds, alternatively spliced.
KJ535024 - Homo sapiens clone PRKAB2_iso-C_adult-A02 AMP-activated protein kinase beta 2 non-catalytic subunit isoform C (PRKAB2) mRNA, complete cds, alternatively spliced.
KJ535025 - Homo sapiens clone PRKAB2_iso-A_fetal-F03 AMP-activated protein kinase beta 2 non-catalytic subunit isoform A (PRKAB2) mRNA, complete cds, alternatively spliced.
AK310982 - Homo sapiens cDNA, FLJ18024.

-  Biochemical and Signaling Pathways
  BioCarta from NCI Cancer Genome Anatomy Project
h_chrebpPathway - ChREBP regulation by carbohydrates and cAMP
h_leptinPathway - Reversal of Insulin Resistance by Leptin

Reactome (by CSHL, EBI, and GO)

Protein O43741 (Reactome details) participates in the following event(s):

R-HSA-163664 AMP binds to gamma subunit of AMP kinase heterotrimer
R-HSA-164151 LKB1 phosphorylates the alpha subunit of AMPK heterotrimer
R-HSA-163691 Phosphorylation of ChREBP at Serine 568 by AMPK
R-HSA-200423 pAMPK inactivates ACACB, inhibiting malonyl-CoA synthesis
R-HSA-380930 Phosphorylated AMPK binds AMP
R-HSA-380949 AMPK is dephosphorylated
R-HSA-200421 Activation of cytosolic AMPK by phosphorylation
R-HSA-6805470 AMPK phosphorylates TP53
R-HSA-5631941 SESN1,2,3 bind AMPK
R-HSA-5672011 p-AMPK:AMP binds the ULK complex
R-HSA-1454699 AMPK-alpha2 phosphorylates TBC1D1
R-HSA-1592244 AMPK phosphorylates PPARGC1A
R-HSA-5665868 AMPK (complex) phosphorylates ULK1 (complex)
R-HSA-5675868 ULK1 phosphorylates ATG13 and RB1CC1
R-HSA-5679239 ULK complex translocates to the ER
R-HSA-380927 p-AMPK phosphorylates TSC1:TSC2
R-HSA-447074 AMPK phosphorylates Raptor in the mTORC1 complex
R-HSA-5673768 p-AMPK:AMP phosphorylates Raptor in the mTORC1 complex
R-HSA-1632857 ULK1 phosphorylates AMBRA1:Beclin-1 complex
R-HSA-5679205 ULK1 phosphorylates Beclin-1
R-HSA-163680 AMPK inhibits chREBP transcriptional activation activity
R-HSA-163685 Energy Metabolism
R-HSA-200425 Import of palmitoyl-CoA into the mitochondrial matrix
R-HSA-380972 Energy dependent regulation of mTOR by LKB1-AMPK
R-HSA-1430728 Metabolism
R-HSA-6804756 Regulation of TP53 Activity through Phosphorylation
R-HSA-8978868 Fatty acid metabolism
R-HSA-5628897 TP53 Regulates Metabolic Genes
R-HSA-1632852 Macroautophagy
R-HSA-165159 mTOR signalling
R-HSA-1445148 Translocation of GLUT4 to the plasma membrane
R-HSA-2151209 Activation of PPARGC1A (PGC-1alpha) by phosphorylation
R-HSA-5633007 Regulation of TP53 Activity
R-HSA-556833 Metabolism of lipids
R-HSA-3700989 Transcriptional Regulation by TP53
R-HSA-8953897 Cellular responses to external stimuli
R-HSA-162582 Signal Transduction
R-HSA-199991 Membrane Trafficking
R-HSA-1592230 Mitochondrial biogenesis
R-HSA-212436 Generic Transcription Pathway
R-HSA-5653656 Vesicle-mediated transport
R-HSA-1852241 Organelle biogenesis and maintenance
R-HSA-73857 RNA Polymerase II Transcription
R-HSA-74160 Gene expression (Transcription)

-  Other Names for This Gene
  Alternate Gene Symbols: A8K9V5, AAKB2_HUMAN, B4DH06, ENST00000254101.1, ENST00000254101.2, ENST00000254101.3, NR_103871, O43741, Q5VXY0, uc317fnf.1, uc317fnf.2
UCSC ID: ENST00000254101.4_7
RefSeq Accession: NM_005399.5
Protein: O43741 (aka AAKB2_HUMAN or AAKC_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.