Human Gene PRKACG (ENST00000377276.5_6) from GENCODE V47lift37
  Description: protein kinase cAMP-activated catalytic subunit gamma (from RefSeq NM_002732.4)
Gencode Transcript: ENST00000377276.5_6
Gencode Gene: ENSG00000165059.8_8
Transcript (Including UTRs)
   Position: hg19 chr9:71,627,420-71,629,029 Size: 1,610 Total Exon Count: 1 Strand: -
Coding Region
   Position: hg19 chr9:71,627,953-71,629,008 Size: 1,056 Coding Exon Count: 1 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
Gene AllelesRNA-Seq ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr9:71,627,420-71,629,029)mRNA (may differ from genome)Protein (351 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
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MalacardsMGIOMIMPubMedReactomeUniProtKB
WikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: KAPCG_HUMAN
DESCRIPTION: RecName: Full=cAMP-dependent protein kinase catalytic subunit gamma; Short=PKA C-gamma; EC=2.7.11.11;
FUNCTION: Phosphorylates a large number of substrates in the cytoplasm and the nucleus.
CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.
ENZYME REGULATION: Activated by cAMP.
SUBUNIT: A number of inactive tetrameric holoenzymes are produced by the combination of homo- or heterodimers of the different regulatory subunits associated with two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits.
TISSUE SPECIFICITY: Testis specific. But important tissues such as brain and ovary have not been analyzed for the content of transcript.
SIMILARITY: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cAMP subfamily.
SIMILARITY: Contains 1 AGC-kinase C-terminal domain.
SIMILARITY: Contains 1 protein kinase domain.
SEQUENCE CAUTION: Sequence=AAC41690.1; Type=Erroneous initiation;
WEB RESOURCE: Name=SeattleSNPs; URL="http://pga.gs.washington.edu/data/prkacg/";

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: PRKACG
Diseases sorted by gene-association score: bleeding disorder, platelet-type, 19* (1730), stormorken syndrome (8), primary pigmented nodular adrenocortical disease (6)
* = Manually curated disease association

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 28.13 RPKM in Testis
Total median expression: 28.16 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -0.8021-0.038 Picture PostScript Text
3' UTR -173.00533-0.325 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR000961 - AGC-kinase_C
IPR011009 - Kinase-like_dom
IPR000719 - Prot_kinase_cat_dom
IPR017441 - Protein_kinase_ATP_BS
IPR002290 - Ser/Thr_dual-sp_kinase_dom
IPR008271 - Ser/Thr_kinase_AS

Pfam Domains:
PF00069 - Protein kinase domain
PF07714 - Protein tyrosine and serine/threonine kinase
PF14531 - Kinase-like

SCOP Domains:
56112 - Protein kinase-like (PK-like)

ModBase Predicted Comparative 3D Structure on P22612
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
Gene SorterGene Sorter    
 RGD    
      
      

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000166 nucleotide binding
GO:0004672 protein kinase activity
GO:0004674 protein serine/threonine kinase activity
GO:0004691 cAMP-dependent protein kinase activity
GO:0005524 ATP binding
GO:0016301 kinase activity
GO:0016740 transferase activity

Biological Process:
GO:0002223 stimulatory C-type lectin receptor signaling pathway
GO:0003091 renal water homeostasis
GO:0006468 protein phosphorylation
GO:0007283 spermatogenesis
GO:0007596 blood coagulation
GO:0008584 male gonad development
GO:0016310 phosphorylation
GO:0034199 activation of protein kinase A activity
GO:0034380 high-density lipoprotein particle assembly
GO:0071377 cellular response to glucagon stimulus

Cellular Component:
GO:0005654 nucleoplasm
GO:0005829 cytosol
GO:0045171 intercellular bridge
GO:0097546 ciliary base


-  Descriptions from all associated GenBank mRNAs
  KJ897378 - Synthetic construct Homo sapiens clone ccsbBroadEn_06772 PRKACG gene, encodes complete protein.
KJ905282 - Synthetic construct Homo sapiens clone ccsbBroadEn_14782 PRKACG gene, encodes complete protein.
M34182 - Human testis-specific protein kinase gamma-subunit mRNA, complete cds.
BC039888 - Homo sapiens protein kinase, cAMP-dependent, catalytic, gamma, mRNA (cDNA clone MGC:47865 IMAGE:5165721), complete cds.
JD066354 - Sequence 47378 from Patent EP1572962.
JD214620 - Sequence 195644 from Patent EP1572962.
JD038153 - Sequence 19177 from Patent EP1572962.
JD244592 - Sequence 225616 from Patent EP1572962.
JD525799 - Sequence 506823 from Patent EP1572962.
JD290141 - Sequence 271165 from Patent EP1572962.
JD262788 - Sequence 243812 from Patent EP1572962.
JD164765 - Sequence 145789 from Patent EP1572962.
JD226699 - Sequence 207723 from Patent EP1572962.
JD545877 - Sequence 526901 from Patent EP1572962.
JD119494 - Sequence 100518 from Patent EP1572962.
JD149704 - Sequence 130728 from Patent EP1572962.
JD352189 - Sequence 333213 from Patent EP1572962.
JD168765 - Sequence 149789 from Patent EP1572962.
JD544293 - Sequence 525317 from Patent EP1572962.
JD551417 - Sequence 532441 from Patent EP1572962.
JD231619 - Sequence 212643 from Patent EP1572962.
JD229914 - Sequence 210938 from Patent EP1572962.
DQ890764 - Synthetic construct clone IMAGE:100003394; FLH165769.01X; RZPDo839G06160D protein kinase, cAMP-dependent, catalytic, gamma (PRKACG) gene, encodes complete protein.
DQ893927 - Synthetic construct Homo sapiens clone IMAGE:100008387; FLH165766.01L; RZPDo839G06159D protein kinase, cAMP-dependent, catalytic, gamma (PRKACG) gene, encodes complete protein.
AB463461 - Synthetic construct DNA, clone: pF1KB6497, Homo sapiens PRKACG gene for cAMP-dependent catalytic protein kinase gamma, without stop codon, in Flexi system.

-  Biochemical and Signaling Pathways
  BioCarta from NCI Cancer Genome Anatomy Project
h_shhPathway - Sonic Hedgehog (Shh) Pathway
h_CSKPathway - Activation of Csk by cAMP-dependent Protein Kinase Inhibits Signaling through the T Cell Receptor
h_ck1Pathway - Regulation of ck1/cdk5 by type 1 glutamate receptors
h_igf1rPathway - Multiple antiapoptotic pathways from IGF-1R signaling lead to BAD phosphorylation
h_nos1Pathway - Nitric Oxide Signaling Pathway
h_GATA3pathway - GATA3 participate in activating the Th2 cytokine genes expression
h_dreampathway - Repression of Pain Sensation by the Transcriptional Regulator DREAM
h_mCalpainPathway - mCalpain and friends in Cell motility
h_vipPathway - Neuropeptides VIP and PACAP inhibit the apoptosis of activated T cells
h_carm1Pathway - Transcription Regulation by Methyltransferase of CARM1
h_nfatPathway - NFAT and Hypertrophy of the heart (Transcription in the broken heart)
h_stathminPathway - Stathmin and breast cancer resistance to antimicrotubule agents
h_akap13Pathway - Rho-Selective Guanine Exchange Factor AKAP13 Mediates Stress Fiber Formation
h_cftrPathway - Cystic fibrosis transmembrane conductance regulator (CFTR) and beta 2 adrenergic receptor (b2AR) pathway
h_crebPathway - Transcription factor CREB and its extracellular signals
h_gpcrPathway - Signaling Pathway from G-Protein Families
h_pparaPathway - Mechanism of Gene Regulation by Peroxisome Proliferators via PPARa(alpha)
h_akap95Pathway - AKAP95 role in mitosis and chromosome dynamics
h_agpcrPathway - Attenuation of GPCR Signaling
h_badPathway - Regulation of BAD phosphorylation
h_chrebpPathway - ChREBP regulation by carbohydrates and cAMP
h_mPRPathway - How Progesterone Initiates the Oocyte Maturation
h_no1Pathway - Actions of Nitric Oxide in the Heart
h_plcePathway - Phospholipase C-epsilon pathway
h_akapCentrosomePathway - Protein Kinase A at the Centrosome

Reactome (by CSHL, EBI, and GO)

Protein P22612 (Reactome details) participates in the following event(s):

R-HSA-111924 PKA catalytic subunit translocates to the nucleus
R-HSA-111925 cAMP induces dissociation of inactive PKA tetramers
R-HSA-381707 PKA:AKAP79:IQGAP1 complex dissociates to active PKA subunits in response to cAMP
R-HSA-5610749 cAMP dissociates PKA, promoting GLI processing
R-HSA-992708 Dual-specific AKAPs bind type I and II PKA regulatory subunits
R-HSA-8951727 cAMP binds PKA tetramer
R-HSA-9010641 AKAP5 recruits PKA to ROBO2
R-HSA-163416 hormone-sensitive lipase (HSL) + 2 ATP -> phosphorylated HSL + 2 ADP
R-HSA-163418 perilipin + 2 ATP -> phosphorylated perilipin + 2 ADP
R-HSA-5682101 PKA phosphorylates 4xPALM-C-p-2S-ABCA1 tetramer
R-HSA-163676 Phosphorylation of pChREBP (Thr 666) at Ser(196) by PKA
R-HSA-163773 Phosphorylation of PF2K-Pase by PKA catalytic subunit
R-HSA-432232 Phosphorylation of Aquaporin-2 by Protein Kinase A (PKA)
R-HSA-177275 PKA phosphorylates DARPP-32 on Thr34
R-HSA-177284 PKA phosphorylates PDE4B
R-HSA-5218854 p-Y420-FYN is phosphorylated on S21
R-HSA-8854908 PKA phosphorylates RET:GDNF:GFRA dimer
R-HSA-111919 PKA phosphorylates CREB
R-HSA-163672 Phosphorylation of ChREBP at Thr(666) by PKA
R-HSA-5687088 PKA phosphorylates MAPKAPK5
R-HSA-443474 Phosphorylation of CREB by PKA
R-HSA-5610717 PKA phosphorylates GLI2
R-HSA-5610720 PKA phosphorylates GLI3
R-HSA-5610741 PKA phosphorylates GLI1
R-HSA-913996 PKA/PKG phosphorylate Rap1GAP2
R-HSA-5624473 Protein kinase A (PKA) and RPS6KA5 (MSK1) phosphorylates p65 (RELA) subunit
R-HSA-111931 PKA-mediated phosphorylation of CREB
R-HSA-163615 PKA activation
R-HSA-164378 PKA activation in glucagon signalling
R-HSA-180024 DARPP-32 events
R-HSA-432040 Vasopressin regulates renal water homeostasis via Aquaporins
R-HSA-381676 Glucagon-like Peptide-1 (GLP1) regulates insulin secretion
R-HSA-5610787 Hedgehog 'off' state
R-HSA-983231 Factors involved in megakaryocyte development and platelet production
R-HSA-9010642 ROBO receptors bind AKAP5
R-HSA-111933 Calmodulin induced events
R-HSA-163359 Glucagon signaling in metabolic regulation
R-HSA-111885 Opioid Signalling
R-HSA-445717 Aquaporin-mediated transport
R-HSA-422356 Regulation of insulin secretion
R-HSA-163560 Triglyceride catabolism
R-HSA-8963896 HDL assembly
R-HSA-163358 PKA-mediated phosphorylation of key metabolic factors
R-HSA-70171 Glycolysis
R-HSA-4420097 VEGFA-VEGFR2 Pathway
R-HSA-8853659 RET signaling
R-HSA-5687128 MAPK6/MAPK4 signaling
R-HSA-442720 CREB phosphorylation through the activation of Adenylate Cyclase
R-HSA-5358351 Signaling by Hedgehog
R-HSA-5610783 Degradation of GLI2 by the proteasome
R-HSA-5610785 GLI3 is processed to GLI3R by the proteasome
R-HSA-5610780 Degradation of GLI1 by the proteasome
R-HSA-109582 Hemostasis
R-HSA-376176 Signaling by ROBO receptors
R-HSA-111997 CaM pathway
R-HSA-163685 Energy Metabolism
R-HSA-418594 G alpha (i) signalling events
R-HSA-382551 Transport of small molecules
R-HSA-8979227 Triglyceride metabolism
R-HSA-8963898 Plasma lipoprotein assembly
R-HSA-70326 Glucose metabolism
R-HSA-392517 Rap1 signalling
R-HSA-194138 Signaling by VEGF
R-HSA-422475 Axon guidance
R-HSA-5621575 CD209 (DC-SIGN) signaling
R-HSA-5683057 MAPK family signaling cascades
R-HSA-438064 Post NMDA receptor activation events
R-HSA-162582 Signal Transduction
R-HSA-111996 Ca-dependent events
R-HSA-1489509 DAG and IP3 signaling
R-HSA-1430728 Metabolism
R-HSA-388396 GPCR downstream signalling
R-HSA-556833 Metabolism of lipids
R-HSA-174824 Plasma lipoprotein assembly, remodeling, and clearance
R-HSA-71387 Metabolism of carbohydrates
R-HSA-1280218 Adaptive Immune System
R-HSA-9006934 Signaling by Receptor Tyrosine Kinases
R-HSA-1266738 Developmental Biology
R-HSA-5621481 C-type lectin receptors (CLRs)
R-HSA-442755 Activation of NMDA receptor and postsynaptic events
R-HSA-112043 PLC beta mediated events
R-HSA-9006925 Intracellular signaling by second messengers
R-HSA-372790 Signaling by GPCR
R-HSA-168256 Immune System
R-HSA-168249 Innate Immune System
R-HSA-112314 Neurotransmitter receptors and postsynaptic signal transmission
R-HSA-112040 G-protein mediated events
R-HSA-112315 Transmission across Chemical Synapses
R-HSA-112316 Neuronal System

-  Other Names for This Gene
  Alternate Gene Symbols: ENST00000377276.1, ENST00000377276.2, ENST00000377276.3, ENST00000377276.4, KAPCG_HUMAN, NM_002732, O60850, P22612, Q5VZ02, Q86YI1, uc318nmw.1, uc318nmw.2
UCSC ID: ENST00000377276.5_6
RefSeq Accession: NM_002732.4
Protein: P22612 (aka KAPCG_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.