Human Gene PRMT3 (ENST00000331079.11_6) from GENCODE V47lift37
  Description: protein arginine methyltransferase 3, transcript variant 1 (from RefSeq NM_005788.4)
Gencode Transcript: ENST00000331079.11_6
Gencode Gene: ENSG00000185238.13_8
Transcript (Including UTRs)
   Position: hg19 chr11:20,409,262-20,530,884 Size: 121,623 Total Exon Count: 16 Strand: +
Coding Region
   Position: hg19 chr11:20,409,293-20,529,959 Size: 120,667 Coding Exon Count: 16 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
Gene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther Species
GO AnnotationsmRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr11:20,409,262-20,530,884)mRNA (may differ from genome)Protein (531 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMalacardsMGIOMIMPubMedReactome
UniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: ANM3_HUMAN
DESCRIPTION: RecName: Full=Protein arginine N-methyltransferase 3; EC=2.1.1.-; AltName: Full=Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 3;
FUNCTION: Methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in some proteins.
ENZYME REGULATION: Inhibited by N-ethylmaleimide and high concentrations of zinc chloride (By similarity).
SUBUNIT: May exist as a monomer or homodimer (By similarity).
SUBCELLULAR LOCATION: Cytoplasm.
DOMAIN: The zinc-finger is responsible for substrate specificity (By similarity).
SIMILARITY: Belongs to the protein arginine N-methyltransferase family.
SIMILARITY: Contains 1 C2H2-type zinc finger.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: PRMT3
Diseases sorted by gene-association score: myopathy of extraocular muscle (9)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 9.47 RPKM in Cells - EBV-transformed lymphocytes
Total median expression: 228.76 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -6.6031-0.213 Picture PostScript Text
3' UTR -196.00925-0.212 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR025799 - Arg_MeTrfase
IPR010456 - Ribosomal-L11_MeTrfase_PrmA
IPR007087 - Znf_C2H2
IPR015880 - Znf_C2H2-like

Pfam Domains:
PF05175 - Methyltransferase small domain
PF06325 - Ribosomal protein L11 methyltransferase (PrmA)
PF08003 - Protein of unknown function (DUF1698)
PF08241 - Methyltransferase domain
PF12756 - C2H2 type zinc-finger (2 copies)
PF13649 - Methyltransferase domain
PF13847 - Methyltransferase domain

SCOP Domains:
51735 - NAD(P)-binding Rossmann-fold domains
53335 - S-adenosyl-L-methionine-dependent methyltransferases
57667 - beta-beta-alpha zinc fingers

Protein Data Bank (PDB) 3-D Structure
MuPIT help
2FYT - X-ray MuPIT 3SMQ - X-ray MuPIT


ModBase Predicted Comparative 3D Structure on O60678
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details Gene Details  
Gene SorterGene Sorter Gene Sorter  
 RGDEnsembl   
      
      

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0003676 nucleic acid binding
GO:0005515 protein binding
GO:0008168 methyltransferase activity
GO:0008469 histone-arginine N-methyltransferase activity
GO:0016274 protein-arginine N-methyltransferase activity
GO:0016740 transferase activity
GO:0035242 protein-arginine omega-N asymmetric methyltransferase activity
GO:0046872 metal ion binding

Biological Process:
GO:0006355 regulation of transcription, DNA-templated
GO:0006479 protein methylation
GO:0019919 peptidyl-arginine methylation, to asymmetrical-dimethyl arginine
GO:0031397 negative regulation of protein ubiquitination
GO:0032259 methylation
GO:0034969 histone arginine methylation
GO:0035246 peptidyl-arginine N-methylation

Cellular Component:
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0005840 ribosome


-  Descriptions from all associated GenBank mRNAs
  BC064831 - Homo sapiens protein arginine methyltransferase 3, mRNA (cDNA clone MGC:72048 IMAGE:6458849), complete cds.
BC019339 - Homo sapiens protein arginine methyltransferase 3, mRNA (cDNA clone IMAGE:3027997), partial cds.
AK129984 - Homo sapiens cDNA FLJ26474 fis, clone KDN04595, highly similar to Protein arginine N-methyltransferase 3 (EC 2.1.1.-).
BC047226 - Homo sapiens cDNA clone IMAGE:5276199, containing frame-shift errors.
AK301449 - Homo sapiens cDNA FLJ61431 complete cds, highly similar to Protein arginine N-methyltransferase 3 (EC 2.1.1.-).
AK298185 - Homo sapiens cDNA FLJ60751 complete cds, highly similar to Protein arginine N-methyltransferase 3 (EC 2.1.1.-).
AK300591 - Homo sapiens cDNA FLJ61411 complete cds, highly similar to Protein arginine N-methyltransferase 3 (EC 2.1.1.-).
BC037544 - Homo sapiens protein arginine methyltransferase 3, mRNA (cDNA clone MGC:40388 IMAGE:5192246), complete cds.
AB590779 - Synthetic construct DNA, clone: pFN21AE1968, Homo sapiens PRMT3 gene for protein arginine methyltransferase 3, without stop codon, in Flexi system.
EU831518 - Synthetic construct Homo sapiens clone HAIB:100066547; DKFZo004B1218 protein arginine methyltransferase 3 protein (PRMT3) gene, encodes complete protein.
EU831425 - Synthetic construct Homo sapiens clone HAIB:100066454; DKFZo008B1217 protein arginine methyltransferase 3 protein (PRMT3) gene, encodes complete protein.
AF059531 - Homo sapiens protein arginine N-methyltransferase 3 (PRMT3) mRNA, partial cds.
AK307776 - Homo sapiens cDNA, FLJ97724.
AK125039 - Homo sapiens cDNA FLJ43049 fis, clone BRTHA3004610, highly similar to Homo sapiens protein arginine N-methyltransferase 3 (PRMT3).
JD459107 - Sequence 440131 from Patent EP1572962.
JD528975 - Sequence 509999 from Patent EP1572962.
JD485973 - Sequence 466997 from Patent EP1572962.
JD355334 - Sequence 336358 from Patent EP1572962.
JD238753 - Sequence 219777 from Patent EP1572962.
JD236825 - Sequence 217849 from Patent EP1572962.
JD508855 - Sequence 489879 from Patent EP1572962.
JD263162 - Sequence 244186 from Patent EP1572962.
JD421343 - Sequence 402367 from Patent EP1572962.
JD084054 - Sequence 65078 from Patent EP1572962.
JD237013 - Sequence 218037 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein O60678 (Reactome details) participates in the following event(s):

R-HSA-8879123 PRMT3 transfers 3xCH3 from 3xAdoMet to RPS2
R-HSA-3214858 RMTs methylate histone arginines
R-HSA-8876725 Protein methylation
R-HSA-3247509 Chromatin modifying enzymes
R-HSA-597592 Post-translational protein modification
R-HSA-4839726 Chromatin organization
R-HSA-392499 Metabolism of proteins

-  Other Names for This Gene
  Alternate Gene Symbols: A0A0A0MSN7, ANM3_HUMAN, B4DUC7, ENST00000331079.1, ENST00000331079.10, ENST00000331079.2, ENST00000331079.3, ENST00000331079.4, ENST00000331079.5, ENST00000331079.6, ENST00000331079.7, ENST00000331079.8, ENST00000331079.9, HRMT1L3 , NM_005788, O60678, PRMT3 , uc317tcx.1, uc317tcx.2
UCSC ID: ENST00000331079.11_6
RefSeq Accession: NM_005788.4
Protein: O60678 (aka ANM3_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.