ID:PRP19_HUMAN DESCRIPTION: RecName: Full=Pre-mRNA-processing factor 19; AltName: Full=Nuclear matrix protein 200; AltName: Full=PRP19/PSO4 homolog; Short=hPso4; AltName: Full=Senescence evasion factor; FUNCTION: Plays a role in DNA double-strand break (DSB) repair. Binds double-stranded DNA in a sequence-nonspecific manner. Acts as a structural component of the nuclear framework. May also serve as a support for spliceosome binding and activity. Essential for spliceosome assembly in a oligomerization-dependent manner and might also be important for spliceosome stability. May have E3 ubiquitin ligase activity. The PSO4 complex is required in the DNA interstrand cross-links (ICLs) repair process. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. SUBUNIT: Homooligomer. Identified in the spliceosome C complex. Component of the PSO4 complex, composed of PRPF19, CDC5L, PLRG1. Component of the PRP19-CDC5L splicing complex composed of a core complex comprising a homotetramer of PRPF19, CDC5L, PLRG1 and BCAS2, and at least three less stably associated proteins CTNNBL1, CWC15 and HSPA8. Interacts in the complex directly with CDC5L, PLRG1 and BCAS2. Interacts with APEX1, DNTT and PSMB4. SUBCELLULAR LOCATION: Nucleus. Nucleus, nucleoplasm. Cytoplasm, cytoskeleton, spindle. Note=Nucleoplasmic in interphase cells. Irregularly distributed in anaphase cells. In prophase cells, uniformly distributed, but not associated with condensing chromosomes. Found in extrachromosomal regions in metaphase cells. Mainly localized to the mitotic spindle apparatus when chromosomes segregate during anaphase. When nuclei reform during late telophase, uniformly distributed in daughter cells and displays no preferred association with decondensing chromatin. TISSUE SPECIFICITY: Ubiquitous. Weakly expressed in senescent cells of different tissue origins. Highly expressed in tumor cell lines. INDUCTION: By gamma irradiation and chemical mutagens but not by UV irradiation. SIMILARITY: Belongs to the WD repeat PRP19 family. SIMILARITY: Contains 1 U-box domain. SIMILARITY: Contains 7 WD repeats.
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on Q9UMS4
Front
Top
Side
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.
Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
Gene Ontology (GO) Annotations with Structured Vocabulary
Molecular Function: GO:0004842 ubiquitin-protein transferase activity GO:0005515 protein binding GO:0016740 transferase activity GO:0034450 ubiquitin-ubiquitin ligase activity GO:0042802 identical protein binding GO:0061630 ubiquitin protein ligase activity
Biological Process: GO:0000209 protein polyubiquitination GO:0000244 spliceosomal tri-snRNP complex assembly GO:0000245 spliceosomal complex assembly GO:0000349 generation of catalytic spliceosome for first transesterification step GO:0000398 mRNA splicing, via spliceosome GO:0001833 inner cell mass cell proliferation GO:0006281 DNA repair GO:0006283 transcription-coupled nucleotide-excision repair GO:0006303 double-strand break repair via nonhomologous end joining GO:0006397 mRNA processing GO:0006974 cellular response to DNA damage stimulus GO:0008380 RNA splicing GO:0008610 lipid biosynthetic process GO:0010498 proteasomal protein catabolic process GO:0016567 protein ubiquitination GO:0034613 cellular protein localization GO:0045665 negative regulation of neuron differentiation GO:0048026 positive regulation of mRNA splicing, via spliceosome GO:0048711 positive regulation of astrocyte differentiation GO:0070534 protein K63-linked ubiquitination GO:0072422 signal transduction involved in DNA damage checkpoint
AJ131186 - Homo sapiens mRNA for nuclear matrix protein NMP200. BC008719 - Homo sapiens PRP19/PSO4 pre-mRNA processing factor 19 homolog (S. cerevisiae), mRNA (cDNA clone MGC:8654 IMAGE:2961655), complete cds. BC018665 - Homo sapiens PRP19/PSO4 pre-mRNA processing factor 19 homolog (S. cerevisiae), mRNA (cDNA clone MGC:2545 IMAGE:2961655), complete cds. BC018698 - Homo sapiens PRP19/PSO4 pre-mRNA processing factor 19 homolog (S. cerevisiae), mRNA (cDNA clone MGC:4240 IMAGE:2961655), complete cds. LF342821 - JP 2014500723-A/150324: Polycomb-Associated Non-Coding RNAs. JD454264 - Sequence 435288 from Patent EP1572962. JD457606 - Sequence 438630 from Patent EP1572962. LF342820 - JP 2014500723-A/150323: Polycomb-Associated Non-Coding RNAs. JD111278 - Sequence 92302 from Patent EP1572962. JD362837 - Sequence 343861 from Patent EP1572962. DQ893003 - Synthetic construct clone IMAGE:100005633; FLH191590.01X; RZPDo839E1177D PRP19/PSO4 pre-mRNA processing factor 19 homolog (S. cerevisiae) (PRPF19) gene, encodes complete protein. KJ893639 - Synthetic construct Homo sapiens clone ccsbBroadEn_03033 PRPF19 gene, encodes complete protein. DQ896249 - Synthetic construct Homo sapiens clone IMAGE:100010709; FLH191586.01L; RZPDo839E1167D PRP19/PSO4 pre-mRNA processing factor 19 homolog (S. cerevisiae) (PRPF19) gene, encodes complete protein. AB527521 - Synthetic construct DNA, clone: pF1KB5909, Homo sapiens PRPF19 gene for PRP19/PSO4 pre-mRNA processing factor 19 homolog, without stop codon, in Flexi system. CU679057 - Synthetic construct Homo sapiens gateway clone IMAGE:100020293 5' read PRPF19 mRNA. LF342819 - JP 2014500723-A/150322: Polycomb-Associated Non-Coding RNAs. LF342818 - JP 2014500723-A/150321: Polycomb-Associated Non-Coding RNAs. AF086469 - Homo sapiens full length insert cDNA clone ZD87A06. JD256351 - Sequence 237375 from Patent EP1572962. JD203322 - Sequence 184346 from Patent EP1572962. JD519024 - Sequence 500048 from Patent EP1572962. JD282838 - Sequence 263862 from Patent EP1572962. JD396962 - Sequence 377986 from Patent EP1572962. MA578398 - JP 2018138019-A/150324: Polycomb-Associated Non-Coding RNAs. MA578397 - JP 2018138019-A/150323: Polycomb-Associated Non-Coding RNAs. MA578396 - JP 2018138019-A/150322: Polycomb-Associated Non-Coding RNAs. MA578395 - JP 2018138019-A/150321: Polycomb-Associated Non-Coding RNAs.
Biochemical and Signaling Pathways
Reactome (by CSHL, EBI, and GO)
Protein Q9UMS4 (Reactome details) participates in the following event(s):
R-HSA-72124 Formation of the Spliceosomal A Complex R-HSA-6782004 Assembly of the pre-incision complex in TC-NER R-HSA-72143 Lariat Formation and 5'-Splice Site Cleavage R-HSA-72139 Formation of the active Spliceosomal C (B*) complex R-HSA-72127 Formation of the Spliceosomal B Complex R-HSA-72130 Formation of an intermediate Spliceosomal C (Bact) complex R-HSA-156661 Formation of Exon Junction Complex R-HSA-6782069 UVSSA:USP7 deubiquitinates ERCC6 R-HSA-6782131 RNA Pol II backtracking in TC-NER R-HSA-6782138 ERCC5 and RPA bind TC-NER site R-HSA-6782211 DNA polymerases delta, epsilon or kappa bind the TC-NER site R-HSA-6782204 5' incision of damaged DNA strand by ERCC1:ERCC4 in TC-NER R-HSA-6782224 3' incision by ERCC5 (XPG) in TC-NER R-HSA-6782227 Ligation of newly synthesized repair patch to incised DNA in TC-NER R-HSA-6782208 Repair DNA synthesis of ~27-30 bases long patch by POLD, POLE or POLK in TC-NER R-HSA-6782141 Binding of ERCC1:ERCC4 (ERCC1:XPF) to pre-incision complex in TC-NER R-HSA-72163 mRNA Splicing - Major Pathway R-HSA-6781823 Formation of TC-NER Pre-Incision Complex R-HSA-6781827 Transcription-Coupled Nucleotide Excision Repair (TC-NER) R-HSA-72172 mRNA Splicing R-HSA-6782135 Dual incision in TC-NER R-HSA-6782210 Gap-filling DNA repair synthesis and ligation in TC-NER R-HSA-5696398 Nucleotide Excision Repair R-HSA-72203 Processing of Capped Intron-Containing Pre-mRNA R-HSA-73894 DNA Repair R-HSA-8953854 Metabolism of RNA
US Server
Sequence and Links to Tools and Databases 
Common Gene Haplotype Alleles 
