Human Gene PRPSAP1 (ENST00000446526.8_7) from GENCODE V47lift37
  Description: phosphoribosyl pyrophosphate synthetase associated protein 1, transcript variant 1 (from RefSeq NM_002766.3)
Gencode Transcript: ENST00000446526.8_7
Gencode Gene: ENSG00000161542.17_16
Transcript (Including UTRs)
   Position: hg19 chr17:74,305,559-74,349,997 Size: 44,439 Total Exon Count: 10 Strand: -
Coding Region
   Position: hg19 chr17:74,307,623-74,349,784 Size: 42,162 Coding Exon Count: 10 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr17:74,305,559-74,349,997)mRNA (may differ from genome)Protein (385 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
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BioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: KPRA_HUMAN
DESCRIPTION: RecName: Full=Phosphoribosyl pyrophosphate synthase-associated protein 1; Short=PRPP synthase-associated protein 1; AltName: Full=39 kDa phosphoribosypyrophosphate synthase-associated protein; Short=PAP39;
FUNCTION: Seems to play a negative regulatory role in 5- phosphoribose 1-diphosphate synthesis.
SUBUNIT: Binds to PRPS1 and PRPS2.
TISSUE SPECIFICITY: Ubiquitous.
SIMILARITY: Belongs to the ribose-phosphate pyrophosphokinase family.
SEQUENCE CAUTION: Sequence=BAG35521.1; Type=Erroneous initiation; Note=Translation N-terminally extended;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 19.73 RPKM in Testis
Total median expression: 430.72 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -106.40213-0.500 Picture PostScript Text
3' UTR -622.902064-0.302 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR005946 - Rib-P_diPkinase

Pfam Domains:
PF13793 - N-terminal domain of ribose phosphate pyrophosphokinase
PF14572 - Phosphoribosyl synthetase-associated domain

SCOP Domains:
51569 - Aldolase
51366 - Ribulose-phoshate binding barrel
51395 - FMN-linked oxidoreductases
51556 - Metallo-dependent hydrolases
53271 - PRTase-like

Protein Data Bank (PDB) 3-D Structure
MuPIT help
2C4K - X-ray MuPIT


ModBase Predicted Comparative 3D Structure on Q14558
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000287 magnesium ion binding
GO:0004749 ribose phosphate diphosphokinase activity
GO:0004857 enzyme inhibitor activity
GO:0005515 protein binding
GO:0042802 identical protein binding

Biological Process:
GO:0006139 nucleobase-containing compound metabolic process
GO:0009165 nucleotide biosynthetic process
GO:0043086 negative regulation of catalytic activity


-  Descriptions from all associated GenBank mRNAs
  BC009012 - Homo sapiens phosphoribosyl pyrophosphate synthetase-associated protein 1, mRNA (cDNA clone MGC:17110 IMAGE:4186365), complete cds.
AK131332 - Homo sapiens cDNA FLJ16342 fis, clone TESTI2015626, highly similar to Human mRNA for phosphoribosypyrophosphate synthetase-associated protein 39.
D61391 - Homo sapiens mRNA for phosphoribosypyrophosphate synthetase-associated protein 39, complete cds.
AK298171 - Homo sapiens cDNA FLJ57408 complete cds, highly similar to Phosphoribosyl pyrophosphatesynthetase-associated protein 1.
AK312637 - Homo sapiens cDNA, FLJ93021, highly similar to Homo sapiens phosphoribosyl pyrophosphate synthetase-associated protein 1 (PRPSAP1), mRNA.
HQ448273 - Synthetic construct Homo sapiens clone IMAGE:100071676; CCSB002821_01 phosphoribosyl pyrophosphate synthetase-associated protein 1 (PRPSAP1) gene, encodes complete protein.
KJ901666 - Synthetic construct Homo sapiens clone ccsbBroadEn_11060 PRPSAP1 gene, encodes complete protein.
CU679229 - Synthetic construct Homo sapiens gateway clone IMAGE:100017083 5' read PRPSAP1 mRNA.
JD112131 - Sequence 93155 from Patent EP1572962.
JD276451 - Sequence 257475 from Patent EP1572962.
JD429756 - Sequence 410780 from Patent EP1572962.
JD467874 - Sequence 448898 from Patent EP1572962.
JD480948 - Sequence 461972 from Patent EP1572962.
JD249887 - Sequence 230911 from Patent EP1572962.
JD511143 - Sequence 492167 from Patent EP1572962.
JD249886 - Sequence 230910 from Patent EP1572962.
JD373784 - Sequence 354808 from Patent EP1572962.
JD344089 - Sequence 325113 from Patent EP1572962.
JD472627 - Sequence 453651 from Patent EP1572962.
JD183354 - Sequence 164378 from Patent EP1572962.
JD507288 - Sequence 488312 from Patent EP1572962.
JD235471 - Sequence 216495 from Patent EP1572962.
JD268072 - Sequence 249096 from Patent EP1572962.
JD488162 - Sequence 469186 from Patent EP1572962.
JD219753 - Sequence 200777 from Patent EP1572962.
JD040421 - Sequence 21445 from Patent EP1572962.
JD434318 - Sequence 415342 from Patent EP1572962.
AK092626 - Homo sapiens cDNA FLJ35307 fis, clone PROST2009901.
JD024453 - Sequence 5477 from Patent EP1572962.

-  Other Names for This Gene
  Alternate Gene Symbols: B2R6M4, ENST00000446526.1, ENST00000446526.2, ENST00000446526.3, ENST00000446526.4, ENST00000446526.5, ENST00000446526.6, ENST00000446526.7, KPRA_HUMAN, NM_002766, Q14558, Q96H06, uc320oev.1, uc320oev.2
UCSC ID: ENST00000446526.8_7
RefSeq Accession: NM_002766.3
Protein: Q14558 (aka KPRA_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.