Human Gene PTGER3 (ENST00000306666.10_10) from GENCODE V47lift37
  Description: prostaglandin E receptor 3, transcript variant 9 (from RefSeq NM_198719.2)
Gencode Transcript: ENST00000306666.10_10
Gencode Gene: ENSG00000050628.21_17
Transcript (Including UTRs)
   Position: hg19 chr1:71,436,465-71,513,499 Size: 77,035 Total Exon Count: 4 Strand: -
Coding Region
   Position: hg19 chr1:71,437,413-71,513,260 Size: 75,848 Coding Exon Count: 4 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
Gene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther Species
GO AnnotationsmRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr1:71,436,465-71,513,499)mRNA (may differ from genome)Protein (390 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMalacardsMGIOMIMPubMedReactome
UniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: PE2R3_HUMAN
DESCRIPTION: RecName: Full=Prostaglandin E2 receptor EP3 subtype; Short=PGE receptor EP3 subtype; Short=PGE2 receptor EP3 subtype; AltName: Full=PGE2-R; AltName: Full=Prostanoid EP3 receptor;
FUNCTION: Receptor for prostaglandin E2 (PGE2); the EP3 receptor may be involved in inhibition of gastric acid secretion, modulation of neurotransmitter release in central and peripheral neurons, inhibition of sodium and water reabsorption in kidney tubulus and contraction in uterine smooth muscle. The activity of this receptor can couple to both the inhibition of adenylate cyclase mediated by G-I proteins, and to an elevation of intracellular calcium. The various isoforms have identical ligand binding properties but can interact with different second messenger systems (By similarity).
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Expressed in small intestine, heart, pancreas, gastric fundic mucosa, mammary artery and pulmonary vessels.
SIMILARITY: Belongs to the G-protein coupled receptor 1 family.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: PTGER3
Diseases sorted by gene-association score: mast cell neoplasm (8), antenatal bartter syndrome (8), chronic conjunctivitis (5), hypersensitivity syndrome, carbamazepine-induced (5), ocular hyperemia (5)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 29.98 RPKM in Uterus
Total median expression: 123.21 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -116.60239-0.488 Picture PostScript Text
3' UTR -206.80948-0.218 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR000276 - 7TM_GPCR_Rhodpsn
IPR001481 - EP3_rcpt_2
IPR017452 - GPCR_Rhodpsn_supfam
IPR008365 - Prostanoid_rcpt
IPR001244 - Prostglndn_DP_rcpt
IPR000265 - Prostglndn_EP3_rcpt

Pfam Domains:
PF00001 - 7 transmembrane receptor (rhodopsin family)

SCOP Domains:
81321 - Family A G protein-coupled receptor-like

ModBase Predicted Comparative 3D Structure on P43115
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
Gene SorterGene Sorter    
 RGD    
      
      

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004871 signal transducer activity
GO:0004930 G-protein coupled receptor activity
GO:0004955 prostaglandin receptor activity
GO:0004957 prostaglandin E receptor activity
GO:0005515 protein binding

Biological Process:
GO:0007165 signal transduction
GO:0007186 G-protein coupled receptor signaling pathway
GO:0007200 phospholipase C-activating G-protein coupled receptor signaling pathway
GO:0008219 cell death
GO:0014827 intestine smooth muscle contraction
GO:0031622 positive regulation of fever generation
GO:0060455 negative regulation of gastric acid secretion

Cellular Component:
GO:0005635 nuclear envelope
GO:0005886 plasma membrane
GO:0005887 integral component of plasma membrane
GO:0016020 membrane
GO:0016021 integral component of membrane


-  Descriptions from all associated GenBank mRNAs
  HQ447638 - Synthetic construct Homo sapiens clone IMAGE:100070984; CCSB013165_02 prostaglandin E receptor 3 (subtype EP3) (PTGER3) gene, encodes complete protein.
X83857 - H.sapiens mRNA for prostaglandin E receptor (EP3a1).
X83859 - H.sapiens mRNA for prostaglandin E receptor (EP3b).
X83861 - H.sapiens mRNA for prostaglandin E receptor (EP3d).
X83863 - H.sapiens mRNA for prostaglandin E receptor (EP3f).
D86097 - Homo sapiens mRNA for prostaglandin EP3 receptor subtype isoform, complete cds.
D86098 - Homo sapiens mRNA for prostaglandin EP3 receptor subtype isoform, complete cds.
U13214 - Human protaglandin receptor EP3F mRNA, complete cds.
U13215 - Human protaglandin receptor EP3E mRNA, complete cds.
BC024229 - Homo sapiens prostaglandin E receptor 3 (subtype EP3), mRNA (cDNA clone MGC:27302 IMAGE:4660371), complete cds.
D38298 - Homo sapiens mRNA for prostaglandin E receotor EP3 subtype 1b isoform, complete cds.
D38301 - Homo sapiens mRNA for prostaglandin E receotor EP3 subtype 3 isoform, complete cds.
L27489 - Homo sapiens prostanoid EP3-III receptor mRNA, complete cds.
D38300 - Homo sapiens mRNA for prostaglandin E receotor EP3 subtype 4 isoform, complete cds.
S69326 - prostaglandin E receptor EP3 subtype [human, erythroleukaemia cells, mRNA, 1488 nt].
AK290634 - Homo sapiens cDNA FLJ77072 complete cds, highly similar to Homo sapiens prostaglandin E receptor 3 (subtype EP3) (PTGER3), transcript variant 9, mRNA.
KJ905881 - Synthetic construct Homo sapiens clone ccsbBroadEn_15551 PTGER3 gene, encodes complete protein.
AY429108 - Homo sapiens prostaglandin E receptor 3 subtype isoform VI mRNA, complete cds.
AY387856 - Homo sapiens prostaglandin E receptor 3 subtype EP3 (PTGER3) mRNA, complete cds; alternatively spliced.
U13216 - Human protaglandin receptor EP3A1 mRNA, complete cds.
BC118578 - Homo sapiens prostaglandin E receptor 3 (subtype EP3), mRNA (cDNA clone MGC:141829 IMAGE:40069637), complete cds.
BC118659 - Homo sapiens prostaglandin E receptor 3 (subtype EP3), mRNA (cDNA clone MGC:141828 IMAGE:40069636), complete cds.
KJ901675 - Synthetic construct Homo sapiens clone ccsbBroadEn_11069 PTGER3 gene, encodes complete protein.
X83862 - H.sapiens mRNA for prostaglandin E receptor (EP3e).
EF534325 - Homo sapiens prostaglandin E receptor 3 subtype EP3 isoform 4 (PTGER3) mRNA, partial cds, alternatively spliced.
X83858 - H.sapiens mRNA for prostaglandin E receptor (EP3a2).
S68874 - EP3 prostanoid receptor EP3-I {3' region, alternatively spliced} [human, mRNA Partial, 598 nt].
U13218 - Human protaglandin receptor EP3A mRNA, complete cds.
L27490 - Homo sapiens prostanoid EP3-I receptor mRNA, complete cds.
D38297 - Homo sapiens mRNA for prostaglandin E receotor EP3 subtype 1a isoform, complete cds.
AK315825 - Homo sapiens cDNA, FLJ79474 complete cds, highly similar to Prostaglandin E2 receptor, EP3 subtype.
L26976 - Human prostaglandin receptor (PGE-2) mRNA, complete cds.
X83860 - H.sapiens mRNA for prostaglandin E receptor (EP3c).
U13217 - Human protaglandin receptor EP3D mRNA, complete cds.
L27488 - Homo sapiens prostanoid EP3-II receptor mRNA, complete cds.
S69200 - EP3 prostanoid receptor isoform EP 3-II {alternatively spliced} [human, mRNA, 1682 nt].
D38299 - Homo sapiens mRNA for prostaglandin E receotor EP3 subtype 2 isoform, complete cds.
AB451481 - Homo sapiens PTGER3 mRNA for prostaglandin E receotor EP3 subtype 3 isoform, partial cds, clone: FLJ80357SAAF.
CU692894 - Synthetic construct Homo sapiens gateway clone IMAGE:100022290 5' read PTGER3 mRNA.
JD458363 - Sequence 439387 from Patent EP1572962.
JD407012 - Sequence 388036 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  BioCarta from NCI Cancer Genome Anatomy Project
h_eicosanoidPathway - Eicosanoid Metabolism

Reactome (by CSHL, EBI, and GO)

Protein P43115 (Reactome details) participates in the following event(s):

R-HSA-391933 EP3 receptor can bind PGE2
R-HSA-749454 The Ligand:GPCR:Gi complex dissociates
R-HSA-749456 Liganded Gi-activating GPCRs bind inactive heterotrimeric G-protein Gi
R-HSA-380073 Liganded Gi-activating GPCR acts as a GEF for Gi
R-HSA-391908 Prostanoid ligand receptors
R-HSA-418594 G alpha (i) signalling events
R-HSA-391903 Eicosanoid ligand-binding receptors
R-HSA-388396 GPCR downstream signalling
R-HSA-373076 Class A/1 (Rhodopsin-like receptors)
R-HSA-372790 Signaling by GPCR
R-HSA-500792 GPCR ligand binding
R-HSA-162582 Signal Transduction

-  Other Names for This Gene
  Alternate Gene Symbols: B0AZN4, B1AK19, B5BUP5, ENST00000306666.1, ENST00000306666.2, ENST00000306666.3, ENST00000306666.4, ENST00000306666.5, ENST00000306666.6, ENST00000306666.7, ENST00000306666.8, ENST00000306666.9, NM_198719, O00326, P43115, PE2R3_HUMAN, Q12943, Q12944, Q12945, Q147X8, Q16546, Q5CZ59, Q5CZ61, Q5CZ62, Q5CZ63, Q5CZ64, uc317nxa.1, uc317nxa.2
UCSC ID: ENST00000306666.10_10
RefSeq Accession: NM_198719.2
Protein: P43115 (aka PE2R3_HUMAN or PE23_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.