ID:PUM2_HUMAN DESCRIPTION: RecName: Full=Pumilio homolog 2; Short=Pumilio-2; FUNCTION: Sequence-specific RNA-binding protein that regulates translation and mRNA stability by binding the 3'-UTR of mRNA targets. Its interactions and tissue specificity suggest that it may be required to support proliferation and self-renewal of stem cells by regulating the translation of key transcripts. SUBUNIT: Homodimer; homodimerizes in vitro. Interacts with DAZ, DAZL and NANOS1 via its pumilio repeats. Binds to a RNA consensus sequence, that is related to the Nanos Response Element (NRE), a 16 bp sequence found in the 3'-UTR of the Drosophila hb mRNA. Also binds to the NRE. Interacts with NANOS3 (By similarity). Interacts with SNAPIN. INTERACTION: Q9NQZ3:DAZ1; NbExp=5; IntAct=EBI-311190, EBI-997955; Q9Q2G4:ORF (xeno); NbExp=3; IntAct=EBI-311190, EBI-6248094; SUBCELLULAR LOCATION: Cytoplasm (Probable). Cytoplasmic granule (By similarity). Cytoplasm, perinuclear region. Note=The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Co-localizes with NANOS3 in the stress granules (By similarity). Co-localizes with NANOS1 and SNAPIN in the perinuclear region of germ cells. TISSUE SPECIFICITY: Expressed in male germ cells of adult testis (at protein level). Highly expressed in testis and ovary. Predominantly expressed in stem cells and germ cells. Expressed at lower level in brain, heart, kidney, liver, muscle, placenta, intestine and stomach Expressed in cerebellum, corpus callosum, caudate nucleus, hippocampus, medulla oblongata and putamen. Expressed in all fetal tissues tested. INDUCTION: Down-regulated in keratinocytes upon UVB irradiation. DOMAIN: The pumilio repeats mediate the association with RNA by packing together to form a right-handed superhelix that approximates a half doughnut. The number as well as the specific sequence of the repeats determine the specificity for target mRNAs (By similarity). SIMILARITY: Contains 1 PUM-HD domain. SIMILARITY: Contains 8 pumilio repeats.
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on Q8TB72
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
Biological Process: GO:0006417 regulation of translation GO:0010608 posttranscriptional regulation of gene expression GO:0034063 stress granule assembly GO:0035196 production of miRNAs involved in gene silencing by miRNA GO:0043488 regulation of mRNA stability GO:0051983 regulation of chromosome segregation GO:0060964 regulation of gene silencing by miRNA GO:1900246 positive regulation of RIG-I signaling pathway GO:2000637 positive regulation of gene silencing by miRNA
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Sequence and Links to Tools and Databases 
Common Gene Haplotype Alleles 
