Human Gene QPCTL (ENST00000012049.10_4) from GENCODE V47lift37
  Description: glutaminyl-peptide cyclotransferase like, transcript variant 1 (from RefSeq NM_017659.4)
Gencode Transcript: ENST00000012049.10_4
Gencode Gene: ENSG00000011478.13_11
Transcript (Including UTRs)
   Position: hg19 chr19:46,195,924-46,207,245 Size: 11,322 Total Exon Count: 7 Strand: +
Coding Region
   Position: hg19 chr19:46,195,962-46,206,307 Size: 10,346 Coding Exon Count: 7 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr19:46,195,924-46,207,245)mRNA (may differ from genome)Protein (382 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMGIPubMedUniProtKBBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: QPCTL_HUMAN
DESCRIPTION: RecName: Full=Glutaminyl-peptide cyclotransferase-like protein; EC=2.3.2.5; AltName: Full=Golgi-resident glutaminyl-peptide cyclotransferase; AltName: Full=isoQC; Short=gQC;
FUNCTION: Responsible for the biosynthesis of pyroglutamyl peptides.
CATALYTIC ACTIVITY: L-glutaminyl-peptide = 5-oxoprolyl-peptide + NH(3).
COFACTOR: Binds 1 zinc ion per subunit.
INTERACTION: Q9GZS1:POLR1E; NbExp=1; IntAct=EBI-1052839, EBI-359458;
SUBCELLULAR LOCATION: Golgi apparatus membrane; Single-pass type I membrane protein.
SIMILARITY: Belongs to the glutaminyl-peptide cyclotransferase family.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 12.43 RPKM in Cells - EBV-transformed lymphocytes
Total median expression: 242.30 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -9.1038-0.239 Picture PostScript Text
3' UTR -591.60938-0.631 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR007484 - Peptidase_M28

Pfam Domains:
PF04389 - Peptidase family M28

SCOP Domains:
53187 - Zn-dependent exopeptidases

Protein Data Bank (PDB) 3-D Structure
MuPIT help
3PB4 - X-ray MuPIT 3PB6 - X-ray MuPIT 3PB7 - X-ray MuPIT 3PB8 - X-ray MuPIT 3PB9 - X-ray MuPIT


ModBase Predicted Comparative 3D Structure on Q9NXS2
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologGenome BrowserNo ortholog
Gene DetailsGene Details  Gene Details 
Gene SorterGene Sorter  Gene Sorter 
 RGD  WormBase 
    Protein Sequence 
    Alignment 

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0008270 zinc ion binding
GO:0016603 glutaminyl-peptide cyclotransferase activity
GO:0016740 transferase activity
GO:0016746 transferase activity, transferring acyl groups
GO:0046872 metal ion binding

Biological Process:
GO:0017186 peptidyl-pyroglutamic acid biosynthetic process, using glutaminyl-peptide cyclotransferase

Cellular Component:
GO:0000139 Golgi membrane
GO:0005794 Golgi apparatus
GO:0016020 membrane
GO:0016021 integral component of membrane


-  Descriptions from all associated GenBank mRNAs
  AK000091 - Homo sapiens cDNA FLJ20084 fis, clone COL03526.
AK172764 - Homo sapiens cDNA FLJ23925 fis, clone COL03589.
AK222636 - Homo sapiens mRNA for glutaminyl-peptide cyclotransferase-like variant, clone: CBL03904.
BC011553 - Homo sapiens glutaminyl-peptide cyclotransferase-like, mRNA (cDNA clone MGC:19993 IMAGE:4564051), complete cds.
AB528634 - Synthetic construct DNA, clone: pF1KB6504, Homo sapiens QPCTL gene for glutaminyl-peptide cyclotransferase-like, without stop codon, in Flexi system.
KJ899018 - Synthetic construct Homo sapiens clone ccsbBroadEn_08412 QPCTL gene, encodes complete protein.
JD266267 - Sequence 247291 from Patent EP1572962.
JD195880 - Sequence 176904 from Patent EP1572962.
JD216421 - Sequence 197445 from Patent EP1572962.
JD310866 - Sequence 291890 from Patent EP1572962.
JD318947 - Sequence 299971 from Patent EP1572962.
JD376777 - Sequence 357801 from Patent EP1572962.
JD417711 - Sequence 398735 from Patent EP1572962.

-  Other Names for This Gene
  Alternate Gene Symbols: ENST00000012049.1, ENST00000012049.2, ENST00000012049.3, ENST00000012049.4, ENST00000012049.5, ENST00000012049.6, ENST00000012049.7, ENST00000012049.8, ENST00000012049.9, NM_017659, Q53HE4, Q96F74, Q9NXS2, QPCTL_HUMAN, uc317bxo.1, uc317bxo.2
UCSC ID: ENST00000012049.10_4
RefSeq Accession: NM_017659.4
Protein: Q9NXS2 (aka QPCTL_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.