Human Gene QTRT2 (ENST00000281273.8_4) from GENCODE V47lift37
  Description: queuine tRNA-ribosyltransferase accessory subunit 2, transcript variant 1 (from RefSeq NM_024638.4)
Gencode Transcript: ENST00000281273.8_4
Gencode Gene: ENSG00000151576.10_13
Transcript (Including UTRs)
   Position: hg19 chr3:113,775,584-113,807,269 Size: 31,686 Total Exon Count: 10 Strand: +
Coding Region
   Position: hg19 chr3:113,784,105-113,804,751 Size: 20,647 Coding Exon Count: 8 

Page IndexSequence and LinksUniProtKB CommentsPrimersGene AllelesRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
PathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr3:113,775,584-113,807,269)mRNA (may differ from genome)Protein (415 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGeneCardsMGI
PubMedReactomeUniProtKBBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: QTRD1_HUMAN
DESCRIPTION: RecName: Full=Queuine tRNA-ribosyltransferase subunit QTRTD1; EC=2.4.2.29; AltName: Full=Queuine tRNA-ribosyltransferase domain-containing protein 1;
FUNCTION: Interacts with QTRT1 to form an active queuine tRNA- ribosyltransferase. This enzyme exchanges queuine for the guanine at the wobble position of tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), thereby forming the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1- yl)amino)methyl)-7-deazaguanosine) (By similarity).
CATALYTIC ACTIVITY: Guanine(34) in tRNA + queuine = queuosine(34) in tRNA + guanine.
COFACTOR: Binds 1 zinc ion per subunit (By similarity).
PATHWAY: tRNA modification; tRNA-queuosine biosynthesis.
SUBUNIT: Interacts with QTRT1 (By similarity).
SUBCELLULAR LOCATION: Cytoplasm (By similarity). Mitochondrion (By similarity). Note=May associate with the mitochondrion outer membrane (By similarity).
SIMILARITY: Belongs to the queuine tRNA-ribosyltransferase family. QTRTD1 subfamily.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 9.68 RPKM in Cells - EBV-transformed lymphocytes
Total median expression: 183.92 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -73.60257-0.286 Picture PostScript Text
3' UTR -722.602518-0.287 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR002616 - tRNA_ribo_trans

Pfam Domains:
PF01702 - Queuine tRNA-ribosyltransferase

SCOP Domains:
51713 - tRNA-guanine transglycosylase

ModBase Predicted Comparative 3D Structure on Q9H974
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologGenome BrowserNo ortholog
Gene DetailsGene Details Gene DetailsGene Details 
Gene SorterGene Sorter Gene SorterGene Sorter 
 RGDEnsembl WormBase 
    Protein Sequence 
    Alignment 

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0008479 queuine tRNA-ribosyltransferase activity
GO:0016763 transferase activity, transferring pentosyl groups
GO:0042803 protein homodimerization activity
GO:0046872 metal ion binding
GO:0046982 protein heterodimerization activity

Biological Process:
GO:0006400 tRNA modification
GO:0008033 tRNA processing
GO:0101030 tRNA-guanine transglycosylation

Cellular Component:
GO:0005737 cytoplasm
GO:0005739 mitochondrion
GO:0005741 mitochondrial outer membrane
GO:0016020 membrane
GO:0032991 macromolecular complex


-  Descriptions from all associated GenBank mRNAs
  AK299313 - Homo sapiens cDNA FLJ54300 complete cds, highly similar to Homo sapiens queuine tRNA-ribosyltransferase domain containing 1 (QTRTD1), mRNA.
BC039265 - Homo sapiens queuine tRNA-ribosyltransferase domain containing 1, mRNA (cDNA clone MGC:33882 IMAGE:5296335), complete cds.
AL833709 - Homo sapiens mRNA; cDNA DKFZp667M199 (from clone DKFZp667M199).
BC034559 - Homo sapiens queuine tRNA-ribosyltransferase domain containing 1, mRNA (cDNA clone MGC:24849 IMAGE:4385906), complete cds.
AK296938 - Homo sapiens cDNA FLJ51987 complete cds, highly similar to Homo sapiens queuine tRNA-ribosyltransferase domain containing 1 (QTRTD1), mRNA.
JD564702 - Sequence 545726 from Patent EP1572962.
AK023022 - Homo sapiens cDNA FLJ12960 fis, clone NT2RP2005605, weakly similar to QUEUINE TRNA-RIBOSYLTRANSFERASE (EC 2.4.2.29).
CR457296 - Homo sapiens full open reading frame cDNA clone RZPDo834C1013D for gene QTRTD1, queuine tRNA-ribosyltransferase domain containing 1; complete cds, incl. stopcodon.
HQ447265 - Synthetic construct Homo sapiens clone IMAGE:100070575; CCSB002164_01 queuine tRNA-ribosyltransferase domain containing 1 (QTRTD1) gene, encodes complete protein.
KJ899552 - Synthetic construct Homo sapiens clone ccsbBroadEn_08946 QTRTD1 gene, encodes complete protein.
AL832215 - Homo sapiens mRNA; cDNA DKFZp686C1636 (from clone DKFZp686C1636).
JD249049 - Sequence 230073 from Patent EP1572962.
JD532503 - Sequence 513527 from Patent EP1572962.
JD490822 - Sequence 471846 from Patent EP1572962.
JD274343 - Sequence 255367 from Patent EP1572962.
JD277377 - Sequence 258401 from Patent EP1572962.
JD291692 - Sequence 272716 from Patent EP1572962.
JD465498 - Sequence 446522 from Patent EP1572962.
JD501323 - Sequence 482347 from Patent EP1572962.
JD279807 - Sequence 260831 from Patent EP1572962.
JD348067 - Sequence 329091 from Patent EP1572962.
JD164971 - Sequence 145995 from Patent EP1572962.
JD454002 - Sequence 435026 from Patent EP1572962.
JD206649 - Sequence 187673 from Patent EP1572962.
JD097847 - Sequence 78871 from Patent EP1572962.
JD447836 - Sequence 428860 from Patent EP1572962.
JD386047 - Sequence 367071 from Patent EP1572962.
JD151174 - Sequence 132198 from Patent EP1572962.
JD357579 - Sequence 338603 from Patent EP1572962.
JD530046 - Sequence 511070 from Patent EP1572962.
JD175793 - Sequence 156817 from Patent EP1572962.
JD316055 - Sequence 297079 from Patent EP1572962.
JD181796 - Sequence 162820 from Patent EP1572962.
JD551222 - Sequence 532246 from Patent EP1572962.
JD289773 - Sequence 270797 from Patent EP1572962.
JD266105 - Sequence 247129 from Patent EP1572962.
JD266106 - Sequence 247130 from Patent EP1572962.
JD353166 - Sequence 334190 from Patent EP1572962.
JD353167 - Sequence 334191 from Patent EP1572962.
JD258874 - Sequence 239898 from Patent EP1572962.
JD152385 - Sequence 133409 from Patent EP1572962.
JD407774 - Sequence 388798 from Patent EP1572962.
JD479118 - Sequence 460142 from Patent EP1572962.
JD479119 - Sequence 460143 from Patent EP1572962.
JD479120 - Sequence 460144 from Patent EP1572962.
JD490658 - Sequence 471682 from Patent EP1572962.
JD111798 - Sequence 92822 from Patent EP1572962.
JD166345 - Sequence 147369 from Patent EP1572962.
JD424539 - Sequence 405563 from Patent EP1572962.
JD451257 - Sequence 432281 from Patent EP1572962.
JD239268 - Sequence 220292 from Patent EP1572962.
JD561996 - Sequence 543020 from Patent EP1572962.
JD221319 - Sequence 202343 from Patent EP1572962.
JD227976 - Sequence 209000 from Patent EP1572962.
JD352323 - Sequence 333347 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  BioCyc Knowledge Library
PWY-8105 - queuosine biosynthesis II (queuine salvage)

Reactome (by CSHL, EBI, and GO)

Protein Q9H974 (Reactome details) participates in the following event(s):

R-HSA-6782443 QTRT1:QTRTD1 exchange guanine for queuosine at guanosine-34 of tRNA(Tyr)
R-HSA-6782315 tRNA modification in the nucleus and cytosol
R-HSA-72306 tRNA processing
R-HSA-8953854 Metabolism of RNA

-  Other Names for This Gene
  Alternate Gene Symbols: A6NGE9, B7Z472, B7Z5R2, ENST00000281273.1, ENST00000281273.2, ENST00000281273.3, ENST00000281273.4, ENST00000281273.5, ENST00000281273.6, ENST00000281273.7, J3KR78, NM_024638, Q6IA59, Q9H974, QTRT2 , QTRT2_HUMAN, QTRTD1, uc317jwc.1, uc317jwc.2
UCSC ID: ENST00000281273.8_4
RefSeq Accession: NM_024638.4
Protein: Q9H974 (aka QTRD1_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.