Human Gene RASAL2 (ENST00000367649.8_6) from GENCODE V47lift37
  Description: RAS protein activator like 2, transcript variant 2 (from RefSeq NM_170692.4)
Gencode Transcript: ENST00000367649.8_6
Gencode Gene: ENSG00000075391.18_15
Transcript (Including UTRs)
   Position: hg19 chr1:178,063,239-178,447,985 Size: 384,747 Total Exon Count: 18 Strand: +
Coding Region
   Position: hg19 chr1:178,063,628-178,442,374 Size: 378,747 Coding Exon Count: 18 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr1:178,063,239-178,447,985)mRNA (may differ from genome)Protein (1280 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMGIOMIMPubMedReactomeUniProtKB
WikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: NGAP_HUMAN
DESCRIPTION: RecName: Full=Ras GTPase-activating protein nGAP; AltName: Full=RAS protein activator-like 2;
FUNCTION: Inhibitory regulator of the Ras-cyclic AMP pathway.
SIMILARITY: Contains 1 C2 domain.
SIMILARITY: Contains 1 PH domain.
SIMILARITY: Contains 1 Ras-GAP domain.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 10.29 RPKM in Artery - Tibial
Total median expression: 220.85 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -206.90389-0.532 Picture PostScript Text
3' UTR -1426.605611-0.254 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR000008 - C2_Ca-dep
IPR008973 - C2_Ca/lipid-bd_dom_CaLB
IPR021887 - DUF3498
IPR011993 - PH_like_dom
IPR001849 - Pleckstrin_homology
IPR001936 - RasGAP
IPR023152 - RasGAP_CS
IPR008936 - Rho_GTPase_activation_prot

Pfam Domains:
PF00168 - C2 domain
PF00616 - GTPase-activator protein for Ras-like GTPase
PF12004 - Domain of unknown function (DUF3498)

SCOP Domains:
48350 - GTPase activation domain, GAP
81790 - Myosin phosphatase inhibitor 17kDa protein, CPI-17
50729 - PH domain-like
49562 - C2 domain (Calcium/lipid-binding domain, CaLB)
52540 - P-loop containing nucleoside triphosphate hydrolases
57997 - Tropomyosin

ModBase Predicted Comparative 3D Structure on Q9UJF2
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
Gene SorterGene Sorter    
 RGDEnsembl   
      
      

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0005096 GTPase activator activity
GO:0005515 protein binding

Biological Process:
GO:0000165 MAPK cascade
GO:0007165 signal transduction
GO:0043087 regulation of GTPase activity
GO:0043547 positive regulation of GTPase activity
GO:0046580 negative regulation of Ras protein signal transduction

Cellular Component:
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0031235 intrinsic component of the cytoplasmic side of the plasma membrane


-  Descriptions from all associated GenBank mRNAs
  AK075169 - Homo sapiens cDNA FLJ90688 fis, clone PLACE1006208, highly similar to Homo sapiens nGAP mRNA.
AK311017 - Homo sapiens cDNA, FLJ18059.
AB209789 - Homo sapiens mRNA for Hypothetical protein protein.
BC078167 - Homo sapiens RAS protein activator like 2, mRNA (cDNA clone IMAGE:6178355), partial cds.
BC063442 - Homo sapiens RAS protein activator like 2, mRNA (cDNA clone IMAGE:5526995), partial cds.
BC110611 - Homo sapiens RAS protein activator like 2, mRNA (cDNA clone MGC:129919 IMAGE:40028914), complete cds.
BC041045 - Homo sapiens, Similar to RAS protein activator like 2, clone IMAGE:4902898, mRNA, partial cds.
AF047711 - Homo sapiens nGAP mRNA, complete cds.
AK290308 - Homo sapiens cDNA FLJ77041 partial cds, highly similar to Homo sapiens RAS protein activator like 2 (RASAL2), transcript variant 1, mRNA.
BC030292 - Homo sapiens RAS protein activator like 2, mRNA (cDNA clone IMAGE:5399841), with apparent retained intron.
JD205341 - Sequence 186365 from Patent EP1572962.
KY966285 - Homo sapiens cell line 93T449 RASAL2/ACVR1 fusion mRNA, partial sequence.
KY966307 - Homo sapiens cell line 94T778 RASAL2/ACVR1 fusion mRNA, partial sequence.
AK022662 - Homo sapiens cDNA FLJ12600 fis, clone NT2RM4001412, highly similar to Homo sapiens nGAP mRNA.
JD432844 - Sequence 413868 from Patent EP1572962.
JD557950 - Sequence 538974 from Patent EP1572962.
JD048461 - Sequence 29485 from Patent EP1572962.
JD124393 - Sequence 105417 from Patent EP1572962.
JD372582 - Sequence 353606 from Patent EP1572962.
JD502855 - Sequence 483879 from Patent EP1572962.
JD441333 - Sequence 422357 from Patent EP1572962.
JD242451 - Sequence 223475 from Patent EP1572962.
JD359036 - Sequence 340060 from Patent EP1572962.
JD491726 - Sequence 472750 from Patent EP1572962.
LF205411 - JP 2014500723-A/12914: Polycomb-Associated Non-Coding RNAs.
LF320219 - JP 2014500723-A/127722: Polycomb-Associated Non-Coding RNAs.
LF320218 - JP 2014500723-A/127721: Polycomb-Associated Non-Coding RNAs.
LF320217 - JP 2014500723-A/127720: Polycomb-Associated Non-Coding RNAs.
MA440988 - JP 2018138019-A/12914: Polycomb-Associated Non-Coding RNAs.
MA555796 - JP 2018138019-A/127722: Polycomb-Associated Non-Coding RNAs.
MA555795 - JP 2018138019-A/127721: Polycomb-Associated Non-Coding RNAs.
MA555794 - JP 2018138019-A/127720: Polycomb-Associated Non-Coding RNAs.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q9UJF2 (Reactome details) participates in the following event(s):

R-HSA-5658435 RAS GAPs bind RAS:GTP
R-HSA-5658231 RAS GAPs stimulate RAS GTPase activity
R-HSA-5658442 Regulation of RAS by GAPs
R-HSA-6802949 Signaling by RAS mutants
R-HSA-5673001 RAF/MAP kinase cascade
R-HSA-6802957 Oncogenic MAPK signaling
R-HSA-5684996 MAPK1/MAPK3 signaling
R-HSA-5663202 Diseases of signal transduction
R-HSA-5683057 MAPK family signaling cascades
R-HSA-1643685 Disease
R-HSA-162582 Signal Transduction

-  Other Names for This Gene
  Alternate Gene Symbols: ENST00000367649.1, ENST00000367649.2, ENST00000367649.3, ENST00000367649.4, ENST00000367649.5, ENST00000367649.6, ENST00000367649.7, F8W755, NGAP, NGAP_HUMAN, NM_170692, O95174, Q2TB22, Q5TFU9, Q9UJF2, uc318gcf.1, uc318gcf.2
UCSC ID: ENST00000367649.8_6
RefSeq Accession: NM_170692.4
Protein: Q9UJF2 (aka NGAP_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.