Human Gene RASGRP3 (ENST00000403687.8_11) from GENCODE V47lift37
  Description: RAS guanyl releasing protein 3, transcript variant 6 (from RefSeq NM_001349977.2)
Gencode Transcript: ENST00000403687.8_11
Gencode Gene: ENSG00000152689.19_20
Transcript (Including UTRs)
   Position: hg19 chr2:33,701,716-33,789,798 Size: 88,083 Total Exon Count: 18 Strand: +
Coding Region
   Position: hg19 chr2:33,740,204-33,787,804 Size: 47,601 Coding Exon Count: 16 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr2:33,701,716-33,789,798)mRNA (may differ from genome)Protein (690 aa)
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BioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: GRP3_HUMAN
DESCRIPTION: RecName: Full=Ras guanyl-releasing protein 3; AltName: Full=Calcium and DAG-regulated guanine nucleotide exchange factor III; AltName: Full=Guanine nucleotide exchange factor for Rap1;
FUNCTION: Guanine nucleotide exchange factor (GEF) for Ras and Rap1.
SIMILARITY: Belongs to the RASGRP family.
SIMILARITY: Contains 2 EF-hand domains.
SIMILARITY: Contains 1 N-terminal Ras-GEF domain.
SIMILARITY: Contains 1 phorbol-ester/DAG-type zinc finger.
SIMILARITY: Contains 1 Ras-GEF domain.
SEQUENCE CAUTION: Sequence=BAA74869.2; Type=Erroneous initiation; Note=Translation N-terminally shortened;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 31.59 RPKM in Cells - EBV-transformed lymphocytes
Total median expression: 143.29 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -62.50319-0.196 Picture PostScript Text
3' UTR -482.101994-0.242 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR020454 - DAG/PE-bd
IPR011992 - EF-hand-like_dom
IPR018247 - EF_Hand_1_Ca_BS
IPR018249 - EF_HAND_2
IPR002048 - EF_hand_Ca-bd
IPR002219 - Prot_Kinase_C-like_PE/DAG-bd
IPR000651 - Ras-like_Gua-exchang_fac_N
IPR008937 - Ras_GEF
IPR023578 - Ras_GEF_dom
IPR001895 - RasGRF_CDC25

Pfam Domains:
PF00130 - Phorbol esters/diacylglycerol binding domain (C1 domain)
PF00617 - RasGEF domain
PF13202 - EF hand

SCOP Domains:
48366 - Ras GEF
47473 - EF-hand
57889 - Cysteine-rich domain

ModBase Predicted Comparative 3D Structure on Q8IV61
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004871 signal transducer activity
GO:0005085 guanyl-nucleotide exchange factor activity
GO:0005088 Ras guanyl-nucleotide exchange factor activity
GO:0005096 GTPase activator activity
GO:0005509 calcium ion binding
GO:0005515 protein binding
GO:0017016 Ras GTPase binding
GO:0019900 kinase binding
GO:0019992 diacylglycerol binding
GO:0046872 metal ion binding

Biological Process:
GO:0000165 MAPK cascade
GO:0007264 small GTPase mediated signal transduction
GO:0007265 Ras protein signal transduction
GO:0035556 intracellular signal transduction
GO:0043087 regulation of GTPase activity
GO:0043547 positive regulation of GTPase activity

Cellular Component:
GO:0005737 cytoplasm
GO:0005886 plasma membrane
GO:0005887 integral component of plasma membrane
GO:0032045 guanyl-nucleotide exchange factor complex
GO:0048471 perinuclear region of cytoplasm


-  Descriptions from all associated GenBank mRNAs
  BC027849 - Homo sapiens RAS guanyl releasing protein 3 (calcium and DAG-regulated), mRNA (cDNA clone MGC:34340 IMAGE:5203041), complete cds.
AK292983 - Homo sapiens cDNA FLJ77595 complete cds, highly similar to Homo sapiens RAS guanyl releasing protein 3 (calcium and DAG-regulated) (RASGRP3), mRNA.
BX647990 - Homo sapiens mRNA; cDNA DKFZp686N2252 (from clone DKFZp686N2252).
JD530691 - Sequence 511715 from Patent EP1572962.
JD262689 - Sequence 243713 from Patent EP1572962.
AB020653 - Homo sapiens KIAA0846 mRNA for KIAA0846 protein.
JD273939 - Sequence 254963 from Patent EP1572962.
JD323837 - Sequence 304861 from Patent EP1572962.
AB384012 - Synthetic construct DNA, clone: pF1KSDA0846, Homo sapiens RASGRP3 gene for RAS guanyl releasing protein 3, complete cds, without stop codon, in Flexi system.
AM392579 - Synthetic construct Homo sapiens clone IMAGE:100002502 for hypothetical protein (RASGRP3 gene).
KJ898545 - Synthetic construct Homo sapiens clone ccsbBroadEn_07939 RASGRP3 gene, encodes complete protein.
AK124316 - Homo sapiens cDNA FLJ42325 fis, clone TRACH3000180.
JD532980 - Sequence 514004 from Patent EP1572962.
JD308496 - Sequence 289520 from Patent EP1572962.
JD328478 - Sequence 309502 from Patent EP1572962.
JD307855 - Sequence 288879 from Patent EP1572962.
JD305110 - Sequence 286134 from Patent EP1572962.
JD501644 - Sequence 482668 from Patent EP1572962.
JD501630 - Sequence 482654 from Patent EP1572962.
JD337871 - Sequence 318895 from Patent EP1572962.
JD285727 - Sequence 266751 from Patent EP1572962.
JD089303 - Sequence 70327 from Patent EP1572962.
JD146559 - Sequence 127583 from Patent EP1572962.
JD499502 - Sequence 480526 from Patent EP1572962.
JD301338 - Sequence 282362 from Patent EP1572962.
JD050476 - Sequence 31500 from Patent EP1572962.
JD485669 - Sequence 466693 from Patent EP1572962.
JD165676 - Sequence 146700 from Patent EP1572962.
JD394289 - Sequence 375313 from Patent EP1572962.
JD158289 - Sequence 139313 from Patent EP1572962.
JD082860 - Sequence 63884 from Patent EP1572962.
JD039979 - Sequence 21003 from Patent EP1572962.
JD039980 - Sequence 21004 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q8IV61 (Reactome details) participates in the following event(s):

R-HSA-1168636 p-RasGRP1,3:DAG cause RAS to exchange GDP for GTP
R-HSA-5672965 RAS GEFs promote RAS nucleotide exchange
R-HSA-1169092 Activation of RAS in B cells
R-HSA-1168372 Downstream signaling events of B Cell Receptor (BCR)
R-HSA-983705 Signaling by the B Cell Receptor (BCR)
R-HSA-1280218 Adaptive Immune System
R-HSA-5673001 RAF/MAP kinase cascade
R-HSA-168256 Immune System
R-HSA-5684996 MAPK1/MAPK3 signaling
R-HSA-5683057 MAPK family signaling cascades
R-HSA-162582 Signal Transduction

-  Other Names for This Gene
  Alternate Gene Symbols: D6W583, ENST00000403687.1, ENST00000403687.2, ENST00000403687.3, ENST00000403687.4, ENST00000403687.5, ENST00000403687.6, ENST00000403687.7, GRP3, GRP3_HUMAN, KIAA0846, NM_001349977, O94931, Q53SD7, Q8IV61, uc319cin.1, uc319cin.2
UCSC ID: ENST00000403687.8_11
RefSeq Accession: NM_001139488.2
Protein: Q8IV61 (aka GRP3_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.