Human Gene RASSF3 (ENST00000542104.6_6) from GENCODE V47lift37
  Description: Ras association domain family member 3, transcript variant 1 (from RefSeq NM_178169.4)
Gencode Transcript: ENST00000542104.6_6
Gencode Gene: ENSG00000153179.14_17
Transcript (Including UTRs)
   Position: hg19 chr12:65,004,275-65,091,344 Size: 87,070 Total Exon Count: 5 Strand: +
Coding Region
   Position: hg19 chr12:65,004,413-65,088,692 Size: 84,280 Coding Exon Count: 5 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
Other NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr12:65,004,275-65,091,344)mRNA (may differ from genome)Protein (238 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMGIOMIMPubMedUniProtKBBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: RASF3_HUMAN
DESCRIPTION: RecName: Full=Ras association domain-containing protein 3;
INTERACTION: Self; NbExp=2; IntAct=EBI-2845202, EBI-2845202; Q13043:STK4; NbExp=2; IntAct=EBI-2845202, EBI-367376;
SUBCELLULAR LOCATION: Cytoplasm. Cytoplasm, cytoskeleton. Note=Localized to microtubules in vascular endothelial cells.
TISSUE SPECIFICITY: Widely expressed.
SIMILARITY: Contains 1 Ras-associating domain.
SIMILARITY: Contains 1 SARAH domain.
CAUTION: Was termed (Ref.1) RASSF5.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 42.17 RPKM in Artery - Tibial
Total median expression: 352.77 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -77.10138-0.559 Picture PostScript Text
3' UTR -703.402652-0.265 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR000159 - Ras-assoc
IPR011524 - SARAH

Pfam Domains:
PF00788 - Ras association (RalGDS/AF-6) domain
PF16517 - Novel Ras effector 1 C-terminal SARAH (Sav/Rassf/Hpo) domain

SCOP Domains:
54236 - Ubiquitin-like

ModBase Predicted Comparative 3D Structure on Q86WH2
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0005515 protein binding
GO:0042802 identical protein binding

Biological Process:
GO:0007165 signal transduction
GO:0042981 regulation of apoptotic process

Cellular Component:
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0005856 cytoskeleton
GO:0005874 microtubule
GO:0005886 plasma membrane


-  Descriptions from all associated GenBank mRNAs
  BC100949 - Homo sapiens Ras association (RalGDS/AF-6) domain family member 3, mRNA (cDNA clone MGC:119194 IMAGE:40004477), complete cds.
BC100950 - Homo sapiens Ras association (RalGDS/AF-6) domain family member 3, mRNA (cDNA clone MGC:119195 IMAGE:40004478), complete cds.
BC100951 - Homo sapiens Ras association (RalGDS/AF-6) domain family member 3, mRNA (cDNA clone MGC:119196 IMAGE:40004481), complete cds.
BC100952 - Homo sapiens Ras association (RalGDS/AF-6) domain family member 3, mRNA (cDNA clone MGC:119197 IMAGE:40004484), complete cds.
AY217662 - Homo sapiens putative RAS binding protein isoform A (RASSF5) mRNA, complete cds; alternatively spliced.
AY217663 - Homo sapiens putative RAS binding protein isoform B (RASSF5) mRNA, complete cds; alternatively spliced.
KJ900547 - Synthetic construct Homo sapiens clone ccsbBroadEn_09941 RASSF3 gene, encodes complete protein.
JD479842 - Sequence 460866 from Patent EP1572962.
JD211383 - Sequence 192407 from Patent EP1572962.
KJ904104 - Synthetic construct Homo sapiens clone ccsbBroadEn_13498 RASSF3 gene, encodes complete protein.
JD164573 - Sequence 145597 from Patent EP1572962.
JD406275 - Sequence 387299 from Patent EP1572962.
JD301293 - Sequence 282317 from Patent EP1572962.

-  Other Names for This Gene
  Alternate Gene Symbols: ENST00000542104.1, ENST00000542104.2, ENST00000542104.3, ENST00000542104.4, ENST00000542104.5, NM_178169, Q86WH1, Q86WH2, RASF3_HUMAN, uc324tok.1, uc324tok.2
UCSC ID: ENST00000542104.6_6
RefSeq Accession: NM_178169.4
Protein: Q86WH2 (aka RASF3_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.