Human Gene RASSF8 (ENST00000689635.1_2) from GENCODE V47lift37
  Description: Ras association domain family member 8, transcript variant 9 (from RefSeq NM_001394098.1)
Gencode Transcript: ENST00000689635.1_2
Gencode Gene: ENSG00000123094.16_16
Transcript (Including UTRs)
   Position: hg19 chr12:26,111,615-26,225,802 Size: 114,188 Total Exon Count: 6 Strand: +
Coding Region
   Position: hg19 chr12:26,208,277-26,221,751 Size: 13,475 Coding Exon Count: 4 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
Gene AllelesMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr12:26,111,615-26,225,802)mRNA (may differ from genome)Protein (419 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblExonPrimerGeneCardsHGNC
MalacardsMGIPubMedUniProtKBWikipedia

-  Comments and Description Text from UniProtKB
  ID: RASF8_HUMAN
DESCRIPTION: RecName: Full=Ras association domain-containing protein 8; AltName: Full=Carcinoma-associated protein HOJ-1;
TISSUE SPECIFICITY: Widely expressed as a 6.2 kb transcript. A 2.2 kb alternatively spliced transcript is expressed exclusively in testis.
DISEASE: Note=A chromosomal aberration involving RASSF8 is found in a complex type of synpolydactyly referred to as 3/3-prime/4 synpolydactyly associated with metacarpal and metatarsal synostoses. Reciprocal translocation t(12;22)(p11.2;q13.3) with C12orf2.
SIMILARITY: Contains 1 Ras-associating domain.
SEQUENCE CAUTION: Sequence=AAC95425.1; Type=Frameshift; Positions=108;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: RASSF8
Diseases sorted by gene-association score: synpolydactyly (24)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
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+  Microarray Expression Data
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-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -367.80669-0.550 Picture PostScript Text
3' UTR -890.904051-0.220 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR000159 - Ras-assoc

Pfam Domains:
PF00788 - Ras association (RalGDS/AF-6) domain

SCOP Domains:
54236 - Ubiquitin-like
57997 - Tropomyosin

Protein Data Bank (PDB) 3-D Structure
MuPIT help
2CS4 - NMR MuPIT


ModBase Predicted Comparative 3D Structure on Q8NHQ8
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
Gene SorterGene Sorter    
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Biological Process:
GO:0007165 signal transduction
GO:0034334 adherens junction maintenance


-  Descriptions from all associated GenBank mRNAs
  BC030021 - Homo sapiens Ras association (RalGDS/AF-6) domain family (N-terminal) member 8, mRNA (cDNA clone MGC:33262 IMAGE:5266519), complete cds.
AB093206 - Homo sapiens mRNA for C12ORF2, complete cds.
AY665468 - Homo sapiens clone B4-E11 carcinoma associated protein HOJ-1 mRNA, complete cds, alternatively spliced.
AK290055 - Homo sapiens cDNA FLJ78723 complete cds.
AK292333 - Homo sapiens cDNA FLJ75219 complete cds.
U82396 - Human carcinoma associated HOJ-1 (HoJ-1) mRNA, complete cds.
CU688808 - Synthetic construct Homo sapiens gateway clone IMAGE:100018201 5' read RASSF8 mRNA.
HQ447900 - Synthetic construct Homo sapiens clone IMAGE:100071256; CCSB002000_02 Ras association (RalGDS/AF-6) domain family 8 (RASSF8) gene, encodes complete protein.
KJ893257 - Synthetic construct Homo sapiens clone ccsbBroadEn_02651 RASSF8 gene, encodes complete protein.
CR456905 - Homo sapiens full open reading frame cDNA clone RZPDo834D057D for gene C12orf2, chromosome 12 open reading frame 2; complete cds, incl. stopcodon.
AY665469 - Homo sapiens carcinoma associated protein HOJ-1 mRNA, partial cds, alternatively spliced.
AY665470 - Homo sapiens carcinoma associated protein HOJ-1 mRNA, partial cds, alternatively spliced.
AY665471 - Homo sapiens carcinoma associated protein HOJ-1 mRNA, partial cds, alternatively spliced.
JD062985 - Sequence 44009 from Patent EP1572962.
JD329991 - Sequence 311015 from Patent EP1572962.
JD233593 - Sequence 214617 from Patent EP1572962.
BC040580 - Homo sapiens cDNA clone IMAGE:5268470.
LF207465 - JP 2014500723-A/14968: Polycomb-Associated Non-Coding RNAs.
AK021604 - Homo sapiens cDNA FLJ11542 fis, clone HEMBA1002801.
LF366487 - JP 2014500723-A/173990: Polycomb-Associated Non-Coding RNAs.
LF366488 - JP 2014500723-A/173991: Polycomb-Associated Non-Coding RNAs.
LF366489 - JP 2014500723-A/173992: Polycomb-Associated Non-Coding RNAs.
JD026213 - Sequence 7237 from Patent EP1572962.
LF366490 - JP 2014500723-A/173993: Polycomb-Associated Non-Coding RNAs.
AL122049 - Homo sapiens mRNA; cDNA DKFZp434O0227 (from clone DKFZp434O0227).
JD021429 - Sequence 2453 from Patent EP1572962.
JD033553 - Sequence 14577 from Patent EP1572962.
MA602064 - JP 2018138019-A/173990: Polycomb-Associated Non-Coding RNAs.
MA602065 - JP 2018138019-A/173991: Polycomb-Associated Non-Coding RNAs.
MA602066 - JP 2018138019-A/173992: Polycomb-Associated Non-Coding RNAs.
MA602067 - JP 2018138019-A/173993: Polycomb-Associated Non-Coding RNAs.
MA443042 - JP 2018138019-A/14968: Polycomb-Associated Non-Coding RNAs.

-  Other Names for This Gene
  Alternate Gene Symbols: A8K1Z0, C12orf2, NM_001394098, O95647, Q5SCI2, Q76KB6, Q8NHQ8, RASF8_HUMAN, uc330tqp.1, uc330tqp.2
UCSC ID: ENST00000689635.1_2
RefSeq Accession: NM_001394098.1
Protein: Q8NHQ8 (aka RASF8_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.