Human Gene RHOC (ENST00000339083.12_8) from GENCODE V47lift37
  Description: ras homolog family member C, transcript variant 1 (from RefSeq NM_175744.5)
Gencode Transcript: ENST00000339083.12_8
Gencode Gene: ENSG00000155366.17_12
Transcript (Including UTRs)
   Position: hg19 chr1:113,243,753-113,249,749 Size: 5,997 Total Exon Count: 6 Strand: -
Coding Region
   Position: hg19 chr1:113,244,162-113,246,421 Size: 2,260 Coding Exon Count: 4 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
Gene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther Species
GO AnnotationsmRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr1:113,243,753-113,249,749)mRNA (may differ from genome)Protein (193 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMalacardsMGIOMIMPubMedReactome
UniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: RHOC_HUMAN
DESCRIPTION: RecName: Full=Rho-related GTP-binding protein RhoC; AltName: Full=Rho cDNA clone 9; Short=h9; Flags: Precursor;
FUNCTION: Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Serves as a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Regulates apical junction formation in bronchial epithelial cells.
SUBUNIT: Interacts with RTKN (By similarity). Interacts with AKAP13, DIAPH1, PKN2, ROCK1 and ROCK2.
SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor; Cytoplasmic side (Potential). Cleavage furrow. Note=Translocates to the equatorial region before furrow formation in a ECT2-dependent manner.
SIMILARITY: Belongs to the small GTPase superfamily. Rho family.
WEB RESOURCE: Name=Atlas of Genetics and Cytogenetics in Oncology and Haematology; URL="http://atlasgeneticsoncology.org/Genes/RHOCID42110ch1p13.html";

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: RHOC
Diseases sorted by gene-association score: breast disease (3), breast cancer (2)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 106.75 RPKM in Artery - Aorta
Total median expression: 2752.87 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -49.50126-0.393 Picture PostScript Text
3' UTR -161.90409-0.396 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR005225 - Small_GTP-bd_dom
IPR001806 - Small_GTPase
IPR003578 - Small_GTPase_Rho

Pfam Domains:
PF00025 - ADP-ribosylation factor family
PF00071 - Ras family
PF08477 - Ras of Complex, Roc, domain of DAPkinase

SCOP Domains:
51735 - NAD(P)-binding Rossmann-fold domains
52540 - P-loop containing nucleoside triphosphate hydrolases

Protein Data Bank (PDB) 3-D Structure
MuPIT help
1Z2C - X-ray MuPIT 2GCN - X-ray MuPIT 2GCO - X-ray MuPIT 2GCP - X-ray MuPIT


ModBase Predicted Comparative 3D Structure on P08134
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
Gene SorterGene Sorter    
 RGD    
      
      

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000166 nucleotide binding
GO:0003924 GTPase activity
GO:0005515 protein binding
GO:0005525 GTP binding

Biological Process:
GO:0000281 mitotic cytokinesis
GO:0007186 G-protein coupled receptor signaling pathway
GO:0007264 small GTPase mediated signal transduction
GO:0032464 positive regulation of protein homooligomerization
GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB signaling
GO:0043297 apical junction assembly
GO:0044319 wound healing, spreading of cells
GO:0051056 regulation of small GTPase mediated signal transduction
GO:0060193 positive regulation of lipase activity
GO:1902766 skeletal muscle satellite cell migration

Cellular Component:
GO:0005622 intracellular
GO:0005634 nucleus
GO:0005829 cytosol
GO:0005886 plasma membrane
GO:0016020 membrane
GO:0032154 cleavage furrow
GO:0032420 stereocilium
GO:0070062 extracellular exosome


-  Descriptions from all associated GenBank mRNAs
  LF206278 - JP 2014500723-A/13781: Polycomb-Associated Non-Coding RNAs.
LF213713 - JP 2014500723-A/21216: Polycomb-Associated Non-Coding RNAs.
BC052808 - Homo sapiens ras homolog gene family, member C, mRNA (cDNA clone MGC:61427 IMAGE:6064555), complete cds.
BC007245 - Homo sapiens ras homolog gene family, member C, mRNA (cDNA clone MGC:15462 IMAGE:2966676), complete cds.
BC009177 - Homo sapiens ras homolog gene family, member C, mRNA (cDNA clone MGC:1448 IMAGE:2988783), complete cds.
GQ900972 - Homo sapiens clone HEL-T-84 epididymis secretory sperm binding protein mRNA, complete cds.
BX648784 - Homo sapiens mRNA; cDNA DKFZp686F07165 (from clone DKFZp686F07165).
AK094474 - Homo sapiens cDNA FLJ37155 fis, clone BRACE2026131, highly similar to Rho-related GTP-binding protein RhoC precursor.
L25081 - Homo sapiens GTPase (rhoC) mRNA, complete cds.
AF498972 - Homo sapiens small GTP binding protein RhoC (ARHC) mRNA, complete cds.
X06821 - Human rhoC mRNA (clone 9).
CU675583 - Synthetic construct Homo sapiens gateway clone IMAGE:100020767 5' read RHOC mRNA.
KJ890707 - Synthetic construct Homo sapiens clone ccsbBroadEn_00101 RHOC gene, encodes complete protein.
BT019448 - Homo sapiens hypothetical protein MGC19531 mRNA, complete cds.
DQ893171 - Synthetic construct clone IMAGE:100005801; FLH194607.01X; RZPDo839D0280D ras homolog gene family, member C (RHOC) gene, encodes complete protein.
DQ896470 - Synthetic construct Homo sapiens clone IMAGE:100010930; FLH194603.01L; RZPDo839D0270D ras homolog gene family, member C (RHOC) gene, encodes complete protein.
CR450360 - Homo sapiens full open reading frame cDNA clone RZPDo834C073D for gene ARHC, ras homolog gene family, member C; complete cds; without stopcodon.
AB528518 - Synthetic construct DNA, clone: pF1KB3630, Homo sapiens RHOC gene for ras homolog gene family, member C, without stop codon, in Flexi system.
MA441855 - JP 2018138019-A/13781: Polycomb-Associated Non-Coding RNAs.
MA449290 - JP 2018138019-A/21216: Polycomb-Associated Non-Coding RNAs.
JD387103 - Sequence 368127 from Patent EP1572962.
JD320831 - Sequence 301855 from Patent EP1572962.
JD203019 - Sequence 184043 from Patent EP1572962.
JD383036 - Sequence 364060 from Patent EP1572962.
LF352906 - JP 2014500723-A/160409: Polycomb-Associated Non-Coding RNAs.
JD293603 - Sequence 274627 from Patent EP1572962.
JD223392 - Sequence 204416 from Patent EP1572962.
LF352905 - JP 2014500723-A/160408: Polycomb-Associated Non-Coding RNAs.
JD041539 - Sequence 22563 from Patent EP1572962.
JD085995 - Sequence 67019 from Patent EP1572962.
JD130284 - Sequence 111308 from Patent EP1572962.
AF085893 - Homo sapiens full length insert cDNA clone YP95A10.
LF352904 - JP 2014500723-A/160407: Polycomb-Associated Non-Coding RNAs.
LF352903 - JP 2014500723-A/160406: Polycomb-Associated Non-Coding RNAs.
MA588483 - JP 2018138019-A/160409: Polycomb-Associated Non-Coding RNAs.
MA588482 - JP 2018138019-A/160408: Polycomb-Associated Non-Coding RNAs.
MA588481 - JP 2018138019-A/160407: Polycomb-Associated Non-Coding RNAs.
MA588480 - JP 2018138019-A/160406: Polycomb-Associated Non-Coding RNAs.
DQ571176 - Homo sapiens piRNA piR-31288, complete sequence.
DQ577103 - Homo sapiens piRNA piR-45215, complete sequence.
JD027197 - Sequence 8221 from Patent EP1572962.
JD029880 - Sequence 10904 from Patent EP1572962.
JD035896 - Sequence 16920 from Patent EP1572962.
JD191891 - Sequence 172915 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein P08134 (Reactome details) participates in the following event(s):

R-HSA-5665748 FMNL2 binds RHOC:GTP
R-HSA-5665761 FMNL3 binds RHOC:GTP
R-HSA-195146 Dissociation of Rho GTP:GDP from GDI complex
R-HSA-5665767 Activated FMNL3 binds G-actin
R-HSA-5625901 CIT (citron kinase) binds activated RHOA, RHOB, RHOC and RAC1
R-HSA-8981443 CIT-3 binds activated RHOA, RHOB, RHOC and RAC1
R-HSA-194894 Rho GTPase:GTP activates downstream effectors
R-HSA-194922 GAPs inactivate Rho GTPase:GTP by hydrolysis
R-HSA-194913 GEFs activate Rho GTPase:GDP
R-HSA-194854 GDIs block activation of Rho GTPase:GDP
R-HSA-419049 ROCK activation by RHO
R-HSA-5666178 RTKN binds RHOA, RHOC or RHOB
R-HSA-416588 Activation of Rho by LARG and PDZ-RhoGEF
R-HSA-419166 GEFs activate RhoA,B,C
R-HSA-5623622 RHOA, RHOB, RHOC and RAC1 bind PKN1, PKN2 or PKN3
R-HSA-5623667 PDPK1 phosphorylates PKN1,2,3
R-HSA-5671970 Activated CIT binds KIF14 and PRC1
R-HSA-5671993 CIT-3 binds DLG4
R-HSA-5623632 RHOA,RHOB,RHOC,RAC1:GTP:PKN1,2,3 binds PDPK1:PIP3
R-HSA-5671919 Activated CIT phosphorylates MRLCs
R-HSA-419083 Myosin phosphatase inactivation by ROCK
R-HSA-419087 LIM kinase phosphorylation by ROCK
R-HSA-419197 Myosin regulatory light chain phosphorylation by ROCK
R-HSA-5663220 RHO GTPases Activate Formins
R-HSA-194840 Rho GTPase cycle
R-HSA-5625900 RHO GTPases activate CIT
R-HSA-195258 RHO GTPase Effectors
R-HSA-416482 G alpha (12/13) signalling events
R-HSA-416572 Sema4D induced cell migration and growth-cone collapse
R-HSA-5627117 RHO GTPases Activate ROCKs
R-HSA-5666185 RHO GTPases Activate Rhotekin and Rhophilins
R-HSA-5625740 RHO GTPases activate PKNs
R-HSA-194315 Signaling by Rho GTPases
R-HSA-388396 GPCR downstream signalling
R-HSA-400685 Sema4D in semaphorin signaling
R-HSA-162582 Signal Transduction
R-HSA-372790 Signaling by GPCR
R-HSA-373755 Semaphorin interactions
R-HSA-422475 Axon guidance
R-HSA-1266738 Developmental Biology

-  Other Names for This Gene
  Alternate Gene Symbols: ARH9, ARHC, B3KSW1, ENST00000339083.1, ENST00000339083.10, ENST00000339083.11, ENST00000339083.2, ENST00000339083.3, ENST00000339083.4, ENST00000339083.5, ENST00000339083.6, ENST00000339083.7, ENST00000339083.8, ENST00000339083.9, NM_175744, P08134, Q6ICN3, RHOC_HUMAN, uc317vft.1, uc317vft.2
UCSC ID: ENST00000339083.12_8
RefSeq Accession: NM_175744.5
Protein: P08134 (aka RHOC_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.