Human Gene RNF149 (ENST00000295317.4_7) from GENCODE V47lift37
  Description: ring finger protein 149 (from RefSeq NM_173647.4)
Gencode Transcript: ENST00000295317.4_7
Gencode Gene: ENSG00000163162.9_9
Transcript (Including UTRs)
   Position: hg19 chr2:101,892,063-101,925,163 Size: 33,101 Total Exon Count: 7 Strand: -
Coding Region
   Position: hg19 chr2:101,893,700-101,925,050 Size: 31,351 Coding Exon Count: 7 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr2:101,892,063-101,925,163)mRNA (may differ from genome)Protein (400 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMGIPubMedUniProtKBBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: RN149_HUMAN
DESCRIPTION: RecName: Full=E3 ubiquitin-protein ligase RNF149; EC=6.3.2.-; AltName: Full=DNA polymerase-transactivated protein 2; AltName: Full=RING finger protein 149; Flags: Precursor;
FUNCTION: E3 ubiquitin-protein ligase. Ubiquitinates BRAF, inducing its proteasomal degradation.
PATHWAY: Protein modification; protein ubiquitination.
SUBCELLULAR LOCATION: Membrane; Single-pass membrane protein (Potential).
DOMAIN: The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme (By similarity).
SIMILARITY: Contains 1 PA (protease associated) domain.
SIMILARITY: Contains 1 RING-type zinc finger.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 98.52 RPKM in Whole Blood
Total median expression: 705.94 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -40.00113-0.354 Picture PostScript Text
3' UTR -395.601637-0.242 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR003137 - Protease-assoc_domain
IPR001841 - Znf_RING
IPR013083 - Znf_RING/FYVE/PHD

Pfam Domains:
PF00097 - Zinc finger, C3HC4 type (RING finger)
PF02225 - PA domain
PF13445 - RING-type zinc-finger
PF13639 - Ring finger domain
PF13923 - Zinc finger, C3HC4 type (RING finger)
PF17123 - RING-like zinc finger

SCOP Domains:
52025 - PA domain
57850 - RING/U-box
57903 - FYVE/PHD zinc finger

ModBase Predicted Comparative 3D Structure on Q8NC42
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0016740 transferase activity
GO:0046872 metal ion binding
GO:0061630 ubiquitin protein ligase activity

Biological Process:
GO:0016567 protein ubiquitination
GO:0031647 regulation of protein stability
GO:0035690 cellular response to drug
GO:0043409 negative regulation of MAPK cascade

Cellular Component:
GO:0016020 membrane
GO:0016021 integral component of membrane


-  Descriptions from all associated GenBank mRNAs
  AK129799 - Homo sapiens cDNA FLJ26288 fis, clone DMC06933.
JD211796 - Sequence 192820 from Patent EP1572962.
L13714 - Human scRNA molecule, transcribed from Alu repeat.
L13713 - Human scRNA molecule, transcribed from Alu repeat.
JD511618 - Sequence 492642 from Patent EP1572962.
AK074985 - Homo sapiens cDNA FLJ90504 fis, clone NT2RP3004090, weakly similar to GOLIATH PROTEIN.
JD556794 - Sequence 537818 from Patent EP1572962.
BC019355 - Homo sapiens ring finger protein 149, mRNA (cDNA clone MGC:13104 IMAGE:3956746), complete cds.
BC032328 - Homo sapiens ring finger protein 149, mRNA (cDNA clone MGC:40400 IMAGE:5106286), complete cds.
AY450390 - Homo sapiens DNA polymerase-transactivated protein 2 (DNAPTP2) mRNA, complete cds.
BC045743 - Homo sapiens ring finger protein 149, mRNA (cDNA clone MGC:48430 IMAGE:5260812), complete cds.
AK075141 - Homo sapiens cDNA FLJ90660 fis, clone PLACE1004887, weakly similar to GOLIATH PROTEIN.
JD188482 - Sequence 169506 from Patent EP1572962.
JD510846 - Sequence 491870 from Patent EP1572962.
JD566964 - Sequence 547988 from Patent EP1572962.
JD026470 - Sequence 7494 from Patent EP1572962.
JD344328 - Sequence 325352 from Patent EP1572962.
AM392566 - Synthetic construct Homo sapiens clone IMAGE:100002609 for hypothetical protein (RNF149 gene).
AB463234 - Synthetic construct DNA, clone: pF1KB8710, Homo sapiens RNF149 gene for ring finger protein 149, without stop codon, in Flexi system.
AK123500 - Homo sapiens cDNA FLJ41506 fis, clone BRTHA2009349.

-  Other Names for This Gene
  Alternate Gene Symbols: DNAPTP2, ENST00000295317.1, ENST00000295317.2, ENST00000295317.3, NM_173647, Q53S14, Q8N5I8, Q8NBY5, Q8NC42, Q8WUU3, RN149_HUMAN, uc317lld.1, uc317lld.2
UCSC ID: ENST00000295317.4_7
RefSeq Accession: NM_173647.4
Protein: Q8NC42 (aka RN149_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.