ID:MCE1_HUMAN DESCRIPTION: RecName: Full=mRNA-capping enzyme; AltName: Full=HCAP1; AltName: Full=HCE; Includes: RecName: Full=Polynucleotide 5'-triphosphatase; EC=3.1.3.33; AltName: Full=mRNA 5'-triphosphatase; Short=TPase; Includes: RecName: Full=mRNA guanylyltransferase; EC=2.7.7.50; AltName: Full=GTP--RNA guanylyltransferase; Short=GTase; FUNCTION: Bifunctional mRNA-capping enzyme exhibiting RNA 5'- triphosphatase activity in the N-terminal part and mRNA guanylyltransferase activity in the C-terminal part. Catalyzes the first two steps of cap formation: by removing the gamma-phosphate from the 5'-triphosphate end of nascent mRNA to yield a diphosphate end, and by transferring the gmp moiety of GTP to the 5'-diphosphate terminus. CATALYTIC ACTIVITY: A 5'-phosphopolynucleotide + H(2)O = a polynucleotide + phosphate. CATALYTIC ACTIVITY: GTP + (5')pp-Pur-mRNA = diphosphate + G(5')ppp-Pur-mRNA. SUBUNIT: Interacts with SUPT5H and RNMT. Interacts with HIV-1 Tat. SUBCELLULAR LOCATION: Nucleus (By similarity). TISSUE SPECIFICITY: Isoform 1 and isoform 4 (at a lesser extent) are expressed in cerebrum, cerebellum, thyroid, lung, heart, liver, kidney, spleen, large intestine, testis, skin and muscle. MISCELLANEOUS: Isoform 2 to isoform 4 lack mRNA 5'- guanylyltransferase activity due to disruptions of the GTase domain. SIMILARITY: In the N-terminal section; belongs to the non-receptor class of the protein-tyrosine phosphatase family. SIMILARITY: In the C-terminal section; belongs to the eukaryotic GTase family.
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on O60942
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
AF025654 - Homo sapiens mRNA capping enzyme (HCE) mRNA, complete cds. BC019954 - Homo sapiens RNA guanylyltransferase and 5'-phosphatase, mRNA (cDNA clone MGC:9536 IMAGE:3923156), complete cds. AB012142 - Homo sapiens hCAP1a mRNA for mRNA capping enzyme, complete cds. AK299777 - Homo sapiens cDNA FLJ50195 complete cds, highly similar to mRNA capping enzyme. AK295719 - Homo sapiens cDNA FLJ51692 complete cds, highly similar to mRNA capping enzyme. AB009022 - Homo sapiens HCE1 mRNA for capping enzyme 1, complete cds. AB009023 - Homo sapiens HCE1A mRNA for capping enzyme 1A, complete cds. AK312407 - Homo sapiens cDNA, FLJ92740, highly similar to Homo sapiens RNA guanylyltransferase and 5'-phosphatase (RNGTT), mRNA. JF432295 - Synthetic construct Homo sapiens clone IMAGE:100073473 RNA guanylyltransferase and 5'-phosphatase (RNGTT) gene, encodes complete protein. KJ897897 - Synthetic construct Homo sapiens clone ccsbBroadEn_07291 RNGTT gene, encodes complete protein. AB385589 - Synthetic construct DNA, clone: pF1KB9518, Homo sapiens RNGTT gene for mRNA-capping enzyme, complete cds, without stop codon, in Flexi system. AB012143 - Homo sapiens hCAP1b mRNA for mRNA capping enzyme, complete cds. AB009024 - Homo sapiens HCE1B mRNA for capping enzyme 1B, complete cds. CU676488 - Synthetic construct Homo sapiens gateway clone IMAGE:100020339 5' read RNGTT mRNA. BX537450 - Homo sapiens mRNA; cDNA DKFZp686J2031 (from clone DKFZp686J2031). JD082665 - Sequence 63689 from Patent EP1572962. JD359561 - Sequence 340585 from Patent EP1572962. JD320310 - Sequence 301334 from Patent EP1572962. JD154785 - Sequence 135809 from Patent EP1572962. JD224060 - Sequence 205084 from Patent EP1572962. JD326091 - Sequence 307115 from Patent EP1572962. JD118148 - Sequence 99172 from Patent EP1572962. JD511002 - Sequence 492026 from Patent EP1572962. JD324056 - Sequence 305080 from Patent EP1572962. JD299150 - Sequence 280174 from Patent EP1572962. JD539730 - Sequence 520754 from Patent EP1572962. JD250053 - Sequence 231077 from Patent EP1572962. JD078275 - Sequence 59299 from Patent EP1572962. JD078699 - Sequence 59723 from Patent EP1572962. JD110150 - Sequence 91174 from Patent EP1572962. JD288505 - Sequence 269529 from Patent EP1572962. JD347363 - Sequence 328387 from Patent EP1572962. JD531496 - Sequence 512520 from Patent EP1572962. JD559160 - Sequence 540184 from Patent EP1572962. JD085619 - Sequence 66643 from Patent EP1572962. JD301835 - Sequence 282859 from Patent EP1572962. JD291707 - Sequence 272731 from Patent EP1572962. JD175063 - Sequence 156087 from Patent EP1572962. JD439042 - Sequence 420066 from Patent EP1572962. JD435211 - Sequence 416235 from Patent EP1572962. JD133774 - Sequence 114798 from Patent EP1572962.
Biochemical and Signaling Pathways
BioCyc Knowledge Library PWY-7375 - mRNA capping I PWY-7379 - mRNA capping II
Reactome (by CSHL, EBI, and GO)
Protein O60942 (Reactome details) participates in the following event(s):
R-HSA-77069 RNA Polymerase II CTD (phosphorylated) binds to CE R-HSA-167128 RNA Polymerase II CTD (phosphorylated) binds to CE R-HSA-77068 Activation of GT R-HSA-77073 SPT5 subunit of Pol II binds the RNA triphosphatase (RTP) R-HSA-77077 Capping complex formation R-HSA-77090 Methylation of GMP-cap by RNA Methyltransferase R-HSA-167133 Activation of GT R-HSA-167153 SPT5 subunit of Pol II binds the RNA triphosphatase (RTP) R-HSA-77078 Hydrolysis of the 5'-end of the nascent transcript by the capping enzyme R-HSA-77081 Formation of the CE:GMP intermediate complex R-HSA-77085 Dissociation of transcript with 5'-GMP from GT R-HSA-77083 Transfer of GMP from the capping enzyme GT site to 5'-end of mRNA R-HSA-72086 mRNA Capping R-HSA-77075 RNA Pol II CTD phosphorylation and interaction with CE R-HSA-167160 RNA Pol II CTD phosphorylation and interaction with CE during HIV infection R-HSA-8953854 Metabolism of RNA R-HSA-73857 RNA Polymerase II Transcription R-HSA-167172 Transcription of the HIV genome R-HSA-74160 Gene expression (Transcription) R-HSA-162599 Late Phase of HIV Life Cycle R-HSA-162587 HIV Life Cycle R-HSA-162906 HIV Infection R-HSA-5663205 Infectious disease R-HSA-1643685 Disease
US Server
Sequence and Links to Tools and Databases 
Common Gene Haplotype Alleles 
