ID:RFA3_HUMAN DESCRIPTION: RecName: Full=Replication protein A 14 kDa subunit; Short=RP-A p14; AltName: Full=Replication factor A protein 3; Short=RF-A protein 3; FUNCTION: Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions. FUNCTION: Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S- phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange. SUBUNIT: Heterotrimer of 70, 32 and 14 kDa chains (canonical replication protein A complex). Component of the alternative replication protein A complex (aRPA) composed of RPA1, RPA3 and RPA4. The DNA-binding activity may reside exclusively on the 70 kDa subunit. Interacts with RPA4. INTERACTION: P43351:RAD52; NbExp=2; IntAct=EBI-621428, EBI-706448; P27694:RPA1; NbExp=4; IntAct=EBI-621428, EBI-621389; P15927:RPA2; NbExp=3; IntAct=EBI-621428, EBI-621404; SUBCELLULAR LOCATION: Nucleus. WEB RESOURCE: Name=NIEHS-SNPs; URL="http://egp.gs.washington.edu/data/rpa3/";
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on P35244
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
BC009868 - Homo sapiens replication protein A3, 14kDa, mRNA (cDNA clone MGC:16404 IMAGE:3940438), complete cds. L07493 - Homo sapiens replication protein A 14kDa subunit (RPA) mRNA, complete cds. BC005264 - Homo sapiens replication protein A3, 14kDa, mRNA (cDNA clone MGC:12305 IMAGE:3826762), complete cds. AK311842 - Homo sapiens cDNA, FLJ92105, Homo sapiens replication protein A3, 14kDa (RPA3), mRNA. KJ904551 - Synthetic construct Homo sapiens clone ccsbBroadEn_13945 RPA3-like gene, encodes complete protein. BT007320 - Homo sapiens replication protein A3, 14kDa mRNA, complete cds. AB464142 - Synthetic construct DNA, clone: pF1KB6783, Homo sapiens RPA3 gene for replication protein A3, without stop codon, in Flexi system. JD324611 - Sequence 305635 from Patent EP1572962. JD367809 - Sequence 348833 from Patent EP1572962. JD149033 - Sequence 130057 from Patent EP1572962. JD132235 - Sequence 113259 from Patent EP1572962. JD394705 - Sequence 375729 from Patent EP1572962. JD186854 - Sequence 167878 from Patent EP1572962. JD067671 - Sequence 48695 from Patent EP1572962. JD445382 - Sequence 426406 from Patent EP1572962. JD473220 - Sequence 454244 from Patent EP1572962. JD381120 - Sequence 362144 from Patent EP1572962. JD360121 - Sequence 341145 from Patent EP1572962. JD412342 - Sequence 393366 from Patent EP1572962. JD361283 - Sequence 342307 from Patent EP1572962. JD223757 - Sequence 204781 from Patent EP1572962. JD503537 - Sequence 484561 from Patent EP1572962. JD283530 - Sequence 264554 from Patent EP1572962.
Biochemical and Signaling Pathways
Reactome (by CSHL, EBI, and GO)
Protein P35244 (Reactome details) participates in the following event(s):
R-HSA-69140 RPA binds to the Flap R-HSA-174445 RPA binds to the Flap on the C-strand R-HSA-176175 Stalling of DNA replication fork and RPA binding R-HSA-5333051 RPA1 binds HSF1 R-HSA-5689317 Formation of the pre-incision complex in GG-NER R-HSA-5693542 Association of RPA complexes with ssDNA at resected DNA DSBs R-HSA-6782138 ERCC5 and RPA bind TC-NER site R-HSA-6788385 The complex of ATR and ATRIP is recruited to ICL-DNA R-HSA-69144 Removal of RNA primer and dissociation of RPA and Dna2 R-HSA-174441 Removal of RNA primer and dissociation of RPA and Dna2 from the C-strand R-HSA-5358579 DNA polymerase delta polymerizes DNA across single stranded gap R-HSA-5687758 PPP4C:PPP4R2 dephosphorylates RPA2 R-HSA-68916 DNA Replication Factor A (RPA) associates with the pre-replicative complex at the origin R-HSA-5686657 ERCC1:XPF cleaves flaps generated by SSA R-HSA-5693561 RAD51 binds BRCA2 at resected DNA DSBs R-HSA-6788392 ATR phosphorylates RPA2, FANCI, FANCD2 and FANCM at ICL-DNA R-HSA-69142 Recruitment of Dna2 endonuclease R-HSA-174451 Recruitment of Dna2 endonuclease to the C strand R-HSA-176101 Recruitment of Rad17-RFC complex to DNA R-HSA-176250 Binding of ATR-ATRIP to the RPA-ssDNA complex R-HSA-110364 PCNA:POLD,POLE:RPA:RFC and FEN1 bind APEX1 R-HSA-5651992 PCNA-containing replication complex binds damaged dsDNA R-HSA-5651809 LIG1, APEX1 and PCNA:POLD,POLE:RPA:RFC dissociate from repaired DNA R-HSA-5653840 POLD,POLE complete replication of damaged DNA after TLS R-HSA-5653766 USP10 binds monoUb:K164,ISG:K164,ISG:K168-PCNA R-HSA-5653754 UBE2L6:TRIM25 ISGylates monoUb:K164-PCNA R-HSA-5653780 USP43 binds ISG:K164,ISG:K168-PCNA R-HSA-5653770 USP10 deubiquitinates monoUb:K164,ISG:K164,ISG:K168-PCNA R-HSA-5653838 POLD,POLE binds deISGylated PCNA after TLS R-HSA-5653786 USP43 deISGylates ISG:K164,ISG:K168-PCNA R-HSA-5655466 USP1:WDR48 deubiquitinates monoUb:K164-PCNA R-HSA-5690991 Binding of ERCC1:ERCC4 (ERCC1:XPF) to pre-incision complex in GG-NER R-HSA-5690213 DNA polymerases delta, epsilon or kappa bind the GG-NER site R-HSA-5690990 5'- incision of DNA by ERCC1:ERCC4 in GG-NER R-HSA-5684875 Binding of ATR:ATRIP to RPA at resected DNA DSBs R-HSA-6782141 Binding of ERCC1:ERCC4 (ERCC1:XPF) to pre-incision complex in TC-NER R-HSA-6782211 DNA polymerases delta, epsilon or kappa bind the TC-NER site R-HSA-6782204 5' incision of damaged DNA strand by ERCC1:ERCC4 in TC-NER R-HSA-5690997 Ligation of newly synthesized repair patch to incised DNA in GG-NER R-HSA-6782227 Ligation of newly synthesized repair patch to incised DNA in TC-NER R-HSA-176264 Recruitment of the Rad9-Hus1-Rad1 complex to DNA R-HSA-110368 POLD,POLE-mediated DNA strand displacement synthesis R-HSA-110371 LIG1 binds APEX1 and PCNA at SSB R-HSA-110363 FEN1 bound to PCNA and APEX1 cleaves flap ssDNA R-HSA-5651805 LIG1 bound to APEX1 and PCNA ligates SSB R-HSA-5652005 RAD18:UBE2B or RBX1:CUL4:DDB1:DTL ubiquitin ligase complex binds PCNA:POLD,POLE:RPA:RFC associated with damaged dsDNA R-HSA-5655481 USP1:WDR48 binds monoUb:K164-PCNA R-HSA-5652009 RAD18:UBE2B or RBX1:CUL4:DDB1:DTL monoubiquitinates PCNA R-HSA-110307 REV1 binds AP-dsDNA R-HSA-110311 POLZ extends translesion synthesis R-HSA-5653756 TRIM25 binds monoUb:164-PCNA R-HSA-110316 POLH binds monoUb:K164-PCNA at damaged TT-CPD-DNA template R-HSA-5654986 SPRTN binds monoUb:K164-PCNA associated with POLH R-HSA-110319 Elongation by POLH R-HSA-5654989 SPRTN:VCP-mediated release of POLH from monoUb:K164-PCNA R-HSA-5655835 POLK forms a quaternary complex with REV1 and POLZ on damaged DNA template R-HSA-5656105 POLI simultaneously binds REV1 and monoUb:K164-PCNA at damaged DNA R-HSA-110317 Insertion of correct bases opposite the lesion by POLH R-HSA-5685011 ATR activation at DNA DSBs R-HSA-5690988 3'-incision of DNA by ERCC5 (XPG) in GG-NER R-HSA-5691001 Repair DNA synthesis of ~27-30 bases long patch by POLD, POLE or POLK in GG-NER R-HSA-6782224 3' incision by ERCC5 (XPG) in TC-NER R-HSA-6782208 Repair DNA synthesis of ~27-30 bases long patch by POLD, POLE or POLK in TC-NER R-HSA-5693593 D-loop extension by DNA polymerases R-HSA-5652151 REV1 recruits POLZ to (AP:Cyt)-DNA Template R-HSA-110308 REV1 inserts dCMP opposite to AP sites in DNA R-HSA-5654985 SPRTN recruits VCP to monoUb:K164-PCNA associated with POLH R-HSA-5655892 POLK incorporates dNMP opposite to damaged DNA base R-HSA-5655965 POLK and POLZ cooperate in elongation of mispaired primer termini R-HSA-5656148 POLI incorporates dNMP opposite to damaged DNA base R-HSA-5656158 POLZ elongates POLI-incorporated dNMP R-HSA-5685156 ATR phosphorylates RPA2 R-HSA-5684887 Activation of CHEK1 at resected DNA DSBs R-HSA-5684882 CHEK1 is recruited to resected DNA DSBs R-HSA-5693580 Association of RAD52 with the RPA complex at resected DNA DSBs R-HSA-6799332 ATR phosphorylates TP53 R-HSA-5693564 Association of RAD51 with RAD52:DNA double-strand break ends R-HSA-5686642 RAD52 promotes single strand annealing at resected DNA DSBs R-HSA-69166 Removal of the Flap Intermediate R-HSA-174437 Removal of the Flap Intermediate from the C-strand R-HSA-176187 Activation of ATR in response to replication stress R-HSA-912446 Meiotic recombination R-HSA-3371453 Regulation of HSF1-mediated heat shock response R-HSA-5358565 Mismatch repair (MMR) directed by MSH2:MSH6 (MutSalpha) R-HSA-5358606 Mismatch repair (MMR) directed by MSH2:MSH3 (MutSbeta) R-HSA-5696395 Formation of Incision Complex in GG-NER R-HSA-5693607 Processing of DNA double-strand break ends R-HSA-6782135 Dual incision in TC-NER R-HSA-6783310 Fanconi Anemia Pathway R-HSA-68962 Activation of the pre-replicative complex R-HSA-5685938 HDR through Single Strand Annealing (SSA) R-HSA-5693616 Presynaptic phase of homologous DNA pairing and strand exchange R-HSA-5651801 PCNA-Dependent Long Patch Base Excision Repair R-HSA-110314 Recognition of DNA damage by PCNA-containing replication complex R-HSA-5656169 Termination of translesion DNA synthesis R-HSA-5696400 Dual Incision in GG-NER R-HSA-5696397 Gap-filling DNA repair synthesis and ligation in GG-NER R-HSA-6782210 Gap-filling DNA repair synthesis and ligation in TC-NER R-HSA-69183 Processive synthesis on the lagging strand R-HSA-174414 Processive synthesis on the C-strand of the telomere R-HSA-69481 G2/M Checkpoints R-HSA-1500620 Meiosis R-HSA-3371556 Cellular response to heat stress R-HSA-5358508 Mismatch Repair R-HSA-5696399 Global Genome Nucleotide Excision Repair (GG-NER) R-HSA-5693567 HDR through Homologous Recombination (HR) or Single Strand Annealing (SSA) R-HSA-6781827 Transcription-Coupled Nucleotide Excision Repair (TC-NER) R-HSA-73894 DNA Repair R-HSA-69002 DNA Replication Pre-Initiation R-HSA-69206 G1/S Transition R-HSA-5693579 Homologous DNA Pairing and Strand Exchange R-HSA-110373 Resolution of AP sites via the multiple-nucleotide patch replacement pathway R-HSA-73893 DNA Damage Bypass R-HSA-110313 Translesion synthesis by Y family DNA polymerases bypasses lesions on DNA template R-HSA-110312 Translesion synthesis by REV1 R-HSA-110320 Translesion Synthesis by POLH R-HSA-5655862 Translesion synthesis by POLK R-HSA-5656121 Translesion synthesis by POLI R-HSA-69186 Lagging Strand Synthesis R-HSA-174417 Telomere C-strand (Lagging Strand) Synthesis R-HSA-69620 Cell Cycle Checkpoints R-HSA-1474165 Reproduction R-HSA-1640170 Cell Cycle R-HSA-2262752 Cellular responses to stress R-HSA-5696398 Nucleotide Excision Repair R-HSA-5693538 Homology Directed Repair R-HSA-68874 M/G1 Transition R-HSA-453279 Mitotic G1-G1/S phases R-HSA-5685942 HDR through Homologous Recombination (HRR) R-HSA-73933 Resolution of Abasic Sites (AP sites) R-HSA-69190 DNA strand elongation R-HSA-180786 Extension of Telomeres R-HSA-8953897 Cellular responses to external stimuli R-HSA-5693532 DNA Double-Strand Break Repair R-HSA-69278 Cell Cycle (Mitotic) R-HSA-69306 DNA Replication R-HSA-73884 Base Excision Repair R-HSA-69473 G2/M DNA damage checkpoint R-HSA-6804756 Regulation of TP53 Activity through Phosphorylation R-HSA-69239 Synthesis of DNA R-HSA-157579 Telomere Maintenance R-HSA-5633007 Regulation of TP53 Activity R-HSA-69242 S Phase R-HSA-73886 Chromosome Maintenance R-HSA-3700989 Transcriptional Regulation by TP53 R-HSA-212436 Generic Transcription Pathway R-HSA-73857 RNA Polymerase II Transcription R-HSA-74160 Gene expression (Transcription)
US Server
Sequence and Links to Tools and Databases 
Common Gene Haplotype Alleles 
