ID:NTKL_HUMAN DESCRIPTION: RecName: Full=N-terminal kinase-like protein; AltName: Full=Coated vesicle-associated kinase of 90 kDa; AltName: Full=SCY1-like protein 1; AltName: Full=Telomerase regulation-associated protein; AltName: Full=Telomerase transcriptional element-interacting factor; AltName: Full=Teratoma-associated tyrosine kinase; FUNCTION: Regulates COPI-mediated retrograde traffic. Has no detectable kinase activity in vitro. FUNCTION: Isoform 6 acts as transcriptional activator. It binds to three different types of GC-rich DNA binding sites (box-A, -B and -C) in the beta-polymerase promoter region. It also binds to the TERT promoter region. SUBUNIT: Interacts with GORAB. Interacts with COPA, COPB1 and COPB2 (By similarity). Homooligomer. Interacts with AP2B1. SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, centrosome. Endoplasmic reticulum-Golgi intermediate compartment. Golgi apparatus, cis-Golgi network. Note=Localized to the Endoplasmic reticulum-Golgi intermediate and cis-Golgi in an ARF1-independent manner. SUBCELLULAR LOCATION: Isoform 1: Cytoplasm. Note=Cytoplasmic throughout the cell cycle. SUBCELLULAR LOCATION: Isoform 2: Cytoplasm. Note=Cytoplasmic throughout the cell cycle. SUBCELLULAR LOCATION: Isoform 3: Cytoplasm. Cytoplasm, cytoskeleton, centrosome. Note=Cytoplasmic during interphase and centrosomal during mitosis, it localizes to the centrosomes in a microtubule-independent manner. SUBCELLULAR LOCATION: Isoform 6: Nucleus. TISSUE SPECIFICITY: Ubiquitous. DOMAIN: The protein kinase domain is predicted to be catalytically inactive. SIMILARITY: Belongs to the protein kinase superfamily. SIMILARITY: Contains 3 HEAT repeats. SIMILARITY: Contains 1 protein kinase domain. SEQUENCE CAUTION: Sequence=AAG09726.1; Type=Frameshift; Positions=289, 307; Sequence=AAG17902.1; Type=Frameshift; Positions=Several;
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
Pfam Domains: PF00069 - Protein kinase domain PF07714 - Protein tyrosine and serine/threonine kinase
SCOP Domains: 48371 - ARM repeat 48431 - Lipovitellin-phosvitin complex, superhelical domain 56112 - Protein kinase-like (PK-like)
ModBase Predicted Comparative 3D Structure on Q96KG9
Front
Top
Side
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.
Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.