ID:SF3B2_HUMAN DESCRIPTION: RecName: Full=Splicing factor 3B subunit 2; AltName: Full=Pre-mRNA-splicing factor SF3b 145 kDa subunit; Short=SF3b145; AltName: Full=SF3b150; AltName: Full=Spliceosome-associated protein 145; Short=SAP 145; FUNCTION: Subunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron. SUBUNIT: Identified in the spliceosome C complex. Component of the U11/U12 snRNPs that are part of the U12-type spliceosome. Component of splicing factor SF3B which is composed of at least eight subunits; SF3B1/SAP155/SF3B155, SF3B2/SAP145/SF3B145, SF3B3/SAP130/SF3B130, SF3B4/SAP49/SF3B49, SF3B14A, PHF5A/SF3B14B, SF3B10 and SF3B125. SF3B associates with the splicing factor SF3A and a 12S RNA unit to form the U2 small nuclear ribonucleoproteins complex (U2 snRNP). SF3B2 interacts directly with SF3B4. Interacts with HIV-1 Vpr. SUBCELLULAR LOCATION: Nucleus (By similarity). PTM: Phosphorylated upon DNA damage, probably by ATM or ATR. SIMILARITY: Contains 1 SAP domain. SEQUENCE CAUTION: Sequence=AAA97461.1; Type=Erroneous initiation; Note=Translation N-terminally extended; Sequence=AAA97461.1; Type=Frameshift; Positions=28; Sequence=AAH53577.1; Type=Miscellaneous discrepancy; Note=Contaminating sequence. Potential poly-A sequence; Sequence=BAG61831.1; Type=Erroneous initiation; Note=Translation N-terminally extended;
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on Q13435
Front
Top
Side
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.
Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
LF211161 - JP 2014500723-A/18664: Polycomb-Associated Non-Coding RNAs. AK290850 - Homo sapiens cDNA FLJ76788 complete cds, highly similar to Homo sapiens splicing factor 3b, subunit 2, 145kDa (SF3B2), mRNA. BC010372 - Homo sapiens, Similar to RIKEN cDNA 2810441F20 gene, clone IMAGE:4294510, mRNA. BC053577 - Homo sapiens splicing factor 3b, subunit 2, 145kDa, mRNA (cDNA clone IMAGE:4478742), partial cds. BX537771 - Homo sapiens mRNA; cDNA DKFZp781L0540 (from clone DKFZp781L0540). AB463285 - Synthetic construct DNA, clone: pF1KB4690, Homo sapiens SF3B2 gene for splicing factor 3b, subunit 2, without stop codon, in Flexi system. BC000401 - Homo sapiens splicing factor 3b, subunit 2, 145kDa, mRNA (cDNA clone IMAGE:2822659), partial cds. U41371 - Human spliceosome associated protein (SAP 145) mRNA, complete cds. LF344356 - JP 2014500723-A/151859: Polycomb-Associated Non-Coding RNAs. AK300016 - Homo sapiens cDNA FLJ56485 complete cds, highly similar to Splicing factor 3B subunit 2. CU675727 - Synthetic construct Homo sapiens gateway clone IMAGE:100018378 5' read SF3B2 mRNA. JF432116 - Synthetic construct Homo sapiens clone IMAGE:100073255 splicing factor 3b, subunit 2, 145kDa (SF3B2) gene, encodes complete protein. KJ902182 - Synthetic construct Homo sapiens clone ccsbBroadEn_11576 SF3B2 gene, encodes complete protein. BC007610 - Homo sapiens splicing factor 3b, subunit 2, 145kDa, mRNA (cDNA clone MGC:15693 IMAGE:3351485), complete cds. JD025549 - Sequence 6573 from Patent EP1572962. BC014125 - Homo sapiens splicing factor 3b, subunit 2, 145kDa, mRNA (cDNA clone IMAGE:4562562), partial cds. BC051237 - Homo sapiens splicing factor 3b, subunit 2, 145kDa, mRNA (cDNA clone IMAGE:6082816), **** WARNING: chimeric clone ****. AK126488 - Homo sapiens cDNA FLJ44524 fis, clone UTERU3003135. AK092510 - Homo sapiens cDNA FLJ35191 fis, clone PLACE6016984. JD278579 - Sequence 259603 from Patent EP1572962. MA579933 - JP 2018138019-A/151859: Polycomb-Associated Non-Coding RNAs. MA446738 - JP 2018138019-A/18664: Polycomb-Associated Non-Coding RNAs.
Biochemical and Signaling Pathways
Reactome (by CSHL, EBI, and GO)
Protein Q13435 (Reactome details) participates in the following event(s):
R-HSA-72124 Formation of the Spliceosomal A Complex R-HSA-75080 Formation of AT-AC A complex R-HSA-75083 ATAC spliceosome mediated 3' splice site cleavage, exon ligation R-HSA-72143 Lariat Formation and 5'-Splice Site Cleavage R-HSA-72139 Formation of the active Spliceosomal C (B*) complex R-HSA-72127 Formation of the Spliceosomal B Complex R-HSA-72130 Formation of an intermediate Spliceosomal C (Bact) complex R-HSA-156661 Formation of Exon Junction Complex R-HSA-75081 Formation of AT-AC B Complex R-HSA-75082 ATAC spliceosome mediated Lariat formation,5' splice site cleavage R-HSA-75079 Formation of AT-AC C complex R-HSA-72163 mRNA Splicing - Major Pathway R-HSA-72165 mRNA Splicing - Minor Pathway R-HSA-72172 mRNA Splicing R-HSA-72203 Processing of Capped Intron-Containing Pre-mRNA R-HSA-8953854 Metabolism of RNA
US Server
Sequence and Links to Tools and Databases 
Common Gene Haplotype Alleles 
