ID:SGOL1_HUMAN DESCRIPTION: RecName: Full=Shugoshin-like 1; Short=hSgo1; AltName: Full=Serologically defined breast cancer antigen NY-BR-85; FUNCTION: Plays a central role in chromosome cohesion during mitosis by preventing premature dissociation of cohesin complex from centromeres after prophase, when most of cohesin complex dissociates from chromosomes arms. May act by preventing phosphorylation of the STAG2 subunit of cohesin complex at the centromere, ensuring cohesin persistence at centromere until cohesin cleavage by ESPL1/separase at anaphase. Essential for proper chromosome segregation during mitosis and this function requires interaction with PPP2R1A. Its phosphorylated form is necessary for chromosome congression and for the proper attachment of spindle microtubule to the kinetochore. Necessary for kinetochore localization of PLK1 and CENPF. May play a role in the tension sensing mechanism of the spindle-assembly checkpoint by regulating PLK1 kinetochore affinity. Isoform 3 plays a role in maintaining centriole cohesion involved in controlling spindle pole integrity. SUBUNIT: Interacts with PPP2CA (or PPP2CB), PPP2R1B, PPP2R5A, PPP2R5B, PPP2R5C, PPP2R5D, PPP2R5E, SET, LRRC59, RBM10 (or RBM5), RPL10A, RPL28, RPL7, RPL7A and RPLP1. Interaction with protein phosphatase 2A occurs most probably through direct binding to the regulatory B56 subunits: PPP2R1B, PPP2R5A, PPP2R5B, PPP2R5C, PPP2R5D, PPP2R5E. Interacts with PPP2R1A and NEK2. Isoform 3 interacts with PLK1. INTERACTION: Q15172:PPP2R5A; NbExp=3; IntAct=EBI-989069, EBI-641666; SUBCELLULAR LOCATION: Nucleus. Chromosome, centromere. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle pole. Cytoplasm, cytoskeleton, centrosome. Note=Localizes to the inner centromere throughout prophase until metaphase and disappears at anaphase. During prometaphase, it localizes to a single focus, while at metaphase, it localizes to 2 spots corresponding to the 2 centromeres. Centromeric localization requires the presence of BUB1 and the interaction with PPP2R1A. Localizes to the inner kinetochore from prophase to early metaphase. Co-localizes with NEK2 and SS18L1 at the kinetochore. Phosphorylation by AUKRB and the presence of BUB1 are required for localization to the kinetochore. Isoform 1 primarily localizes to kinetochores during G2 phase and mitotic prophase, metaphase, and anaphase and does not appear to be associated with kinetochores during late mitosis. Isoform 3 is found at the centrosome in interphase and at spindle poles in mitosis and its spindle pole localization is PLK1 dependent. Isoform 3 does not localize to kinetochores during any stages of the cell cycle. TISSUE SPECIFICITY: Widely expressed. Highly expressed in testis. Expressed in lung, small intestine, breast, liver and placenta. Strongly overexpressed in 90% of breast cancers tested. DEVELOPMENTAL STAGE: Appears in prophase cells and remains present until metaphase. Strongly decreases at the onset of anaphase and completely disappears at telophase. Not present in interphase cells (at protein level). DOMAIN: The KEN box and D-box 3 are required for its ubiquitination and degradation. PTM: Ubiquitinated and degraded during mitotic exit by APC/C-Cdh1. PTM: Phosphorylation by NEK2 is essential for chromosome congression in mitosis and for the proper attachment of spindle microtubule to the kinetochore. Phosphorylated by PLK1 and AUKRB. MISCELLANEOUS: Shugoshin is Japanese for guardian spirit (as it is known to be a protector of centromeric cohesin). SIMILARITY: Belongs to the shugoshin family. SEQUENCE CAUTION: Sequence=AAH01339.2; Type=Erroneous initiation; Sequence=AAH32696.1; Type=Erroneous initiation;
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on Q5FBB7
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
AB187578 - Homo sapiens SgoL1-A2 mRNA for shugoshin-like protein 1, transcript variant A2, complete cds. AB187580 - Homo sapiens SgoL1-B2 mRNA for shugoshin-like protein 1, transcript variant B2, complete cds. AB187582 - Homo sapiens SgoL1-C2 mRNA for shugoshin-like protein 1, transcript variant C2, complete cds. BC017867 - Homo sapiens shugoshin-like 1 (S. pombe), mRNA (cDNA clone MGC:22640 IMAGE:4685140), complete cds. JD354815 - Sequence 335839 from Patent EP1572962. JD232541 - Sequence 213565 from Patent EP1572962. JD234740 - Sequence 215764 from Patent EP1572962. JD553353 - Sequence 534377 from Patent EP1572962. AB193060 - Homo sapiens Sgo1 mRNA for shugoshin 1EF protein, complete cds. AB193061 - Homo sapiens Sgo1 mRNA for shugoshin 1EF protein, complete cds. AB193062 - Homo sapiens Sgo1 mRNA for shugoshin 1GH protein, complete cds. AB193063 - Homo sapiens Sgo1 mRNA for shugoshin 1GH protein, complete cds. AB193064 - Homo sapiens Sgo1 mRNA for shugoshin 1J protein, complete cds. AB193065 - Homo sapiens Sgo1 mRNA for shugoshin 1KL protein, complete cds. AB193066 - Homo sapiens Sgo1 mRNA for shugoshin 1KL protein, complete cds. KJ895662 - Synthetic construct Homo sapiens clone ccsbBroadEn_05056 SGOL1 gene, encodes complete protein. BX648516 - Homo sapiens mRNA; cDNA DKFZp686J0878 (from clone DKFZp686J0878). BC032696 - Homo sapiens shugoshin-like 1 (S. pombe), mRNA (cDNA clone IMAGE:5416447), partial cds. BC001339 - Homo sapiens shugoshin-like 1 (S. pombe), mRNA (cDNA clone IMAGE:3461987), partial cds. AB190994 - Homo sapiens Sgo1 mRNA for shugoshin-like 1, complete cds. AB187577 - Homo sapiens SgoL1-A1 mRNA for shugoshin-like protein 1, transcript variant A1, complete cds. AB187579 - Homo sapiens SgoL1-B1 mRNA for shugoshin-like protein 1, transcript variant B1, complete cds. AB187581 - Homo sapiens SgoL1-C1 mRNA for shugoshin-like protein 1, transcript variant C1, complete cds. AB567656 - Homo sapiens SGOL1 mRNA for shugoshin-like 1 isoform P1, complete CDS, like variant C1. AB567657 - Homo sapiens SGOL1 mRNA for shugoshin-like 1 isoform P1, complete CDS, like variant B1. BC039605 - Homo sapiens shugoshin-like 1 (S. pombe), mRNA (cDNA clone IMAGE:3861301), partial cds. AF308299 - Homo sapiens serologically defined breast cancer antigen NY-BR-85 mRNA, partial cds. AB193056 - Homo sapiens Sgo1 mRNA for shugoshin 1AB protein, complete cds. AB193057 - Homo sapiens Sgo1 mRNA for shugoshin 1AB protein, complete cds. AB193058 - Homo sapiens Sgo1 mRNA for shugoshin 1CD protein, complete cds. AB193059 - Homo sapiens Sgo1 mRNA for shugoshin 1CD protein, complete cds. AB451340 - Homo sapiens SGOL1 mRNA for shugoshin-like 1 isoform A1, complete cds, clone: FLJ85508SAAN. AB451489 - Homo sapiens SGOL1 mRNA for shugoshin-like 1 isoform A1, partial cds, clone: FLJ85508SAAF. AK308095 - Homo sapiens cDNA, FLJ98043. JD195460 - Sequence 176484 from Patent EP1572962.
Biochemical and Signaling Pathways
Reactome (by CSHL, EBI, and GO)
Protein Q5FBB7 (Reactome details) participates in the following event(s):
R-HSA-141409 Mad1 binds kinetochore R-HSA-375302 Kinetochore capture of astral microtubules R-HSA-5666129 CDC42:GTP recruits DIAPH2-2 to kinetochores R-HSA-5666169 Kinetochore capture of astral microtubules is positively regulated by CDC42:GTP:p-S196-DIAPH2-2 R-HSA-141431 MAD2 associates with the Mad1 kinetochore complex R-HSA-141439 Release of activated MAD2 from kinetochores R-HSA-2467811 Separation of sister chromatids R-HSA-2467809 ESPL1 (Separase) cleaves centromeric cohesin R-HSA-5666160 AURKB phosphorylates DIAPH2-2 at kinetochores R-HSA-141422 MAD2 converted to an inhibitory state via interaction with Mad1 R-HSA-1638821 PP2A-B56 dephosphorylates centromeric cohesin R-HSA-1638803 Phosphorylation of cohesin by PLK1 at centromeres R-HSA-2468287 CDK1 phosphorylates CDCA5 (Sororin) at centromeres R-HSA-141444 Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal R-HSA-68877 Mitotic Prometaphase R-HSA-5663220 RHO GTPases Activate Formins R-HSA-2500257 Resolution of Sister Chromatid Cohesion R-HSA-2467813 Separation of Sister Chromatids R-HSA-141424 Amplification of signal from the kinetochores R-HSA-68886 M Phase R-HSA-195258 RHO GTPase Effectors R-HSA-68882 Mitotic Anaphase R-HSA-69618 Mitotic Spindle Checkpoint R-HSA-69278 Cell Cycle (Mitotic) R-HSA-194315 Signaling by Rho GTPases R-HSA-2555396 Mitotic Metaphase and Anaphase R-HSA-69620 Cell Cycle Checkpoints R-HSA-1640170 Cell Cycle R-HSA-162582 Signal Transduction
US Server
Sequence and Links to Tools and Databases 
Common Gene Haplotype Alleles 
