ID:SP100_HUMAN DESCRIPTION: RecName: Full=Nuclear autoantigen Sp-100; AltName: Full=Lysp100b; AltName: Full=Nuclear dot-associated Sp100 protein; AltName: Full=Speckled 100 kDa; FUNCTION: May play a role in the control of gene expression. SUBUNIT: Homodimer. Splice variants heterodimerize. Interacts with members of the HP1 family of nonhistone chromosomal protein, such as CBX5 and CBX3 via the PxVxL motif. Interacts with Epstein-Barr virus EBNA-LP. Interacts with human cytomegalovirus/HHV-5 protein UL123. INTERACTION: P55854:SUMO3; NbExp=2; IntAct=EBI-751145, EBI-474067; SUBCELLULAR LOCATION: Nucleus, PML body. Note=Found in the nuclear body, also known as nuclear domain 10 (ND10), PML oncogenic domain (POD), nuclear dots (ND) and KR body. The nuclear body is a nucleoplasmic structure of punctate shape, which varies in size and number. Induction by interferon and may be cell cycle stages modulate the subnuclear localization of the isoforms. TISSUE SPECIFICITY: Widely expressed. Sp100-B is expressed only in spleen, tonsil, thymus, mature B-cell line and some T-cell line, but not in brain, liver, muscle or non-lymphoid cell lines. INDUCTION: By interferon. DOMAIN: The HSR domain is important for the nuclear body targeting as well as for the dimerization. DOMAIN: Contains one Pro-Xaa-Val-Xaa-Leu (PxVxL) motif, which is required for interaction with chromoshadow domains. This motif requires additional residues -7, -6, +4 and +5 of the central Val which contact the chromoshadow domain. PTM: Sumoylated. Sumoylation depends on a functional nuclear localization signal but is not necessary for nuclear import or nuclear body targeting. MISCELLANEOUS: The major isoform Sp100-A, has a calculated MW of 54 kDa, but exhibits aberrant electrophoretic mobilities, with an apparent MW OF 100 kDa. SIMILARITY: Contains 2 HMG box DNA-binding domains. SIMILARITY: Contains 1 HSR domain. SIMILARITY: Contains 1 SAND domain.
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on P23497
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
Gene Ontology (GO) Annotations with Structured Vocabulary
Molecular Function: GO:0000981 RNA polymerase II transcription factor activity, sequence-specific DNA binding GO:0003677 DNA binding GO:0003713 transcription coactivator activity GO:0003714 transcription corepressor activity GO:0005515 protein binding GO:0008134 transcription factor binding GO:0019900 kinase binding GO:0019904 protein domain specific binding GO:0042802 identical protein binding GO:0042803 protein homodimerization activity GO:0070087 chromo shadow domain binding
Biological Process: GO:0000122 negative regulation of transcription from RNA polymerase II promoter GO:0000723 telomere maintenance GO:0006351 transcription, DNA-templated GO:0006355 regulation of transcription, DNA-templated GO:0006978 DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator GO:0010596 negative regulation of endothelial cell migration GO:0016032 viral process GO:0032526 response to retinoic acid GO:0032897 negative regulation of viral transcription GO:0034097 response to cytokine GO:0034340 response to type I interferon GO:0034341 response to interferon-gamma GO:0043392 negative regulation of DNA binding GO:0043433 negative regulation of sequence-specific DNA binding transcription factor activity GO:0045185 maintenance of protein location GO:0045765 regulation of angiogenesis GO:0045892 negative regulation of transcription, DNA-templated GO:0045893 positive regulation of transcription, DNA-templated GO:0046826 negative regulation of protein export from nucleus GO:0048384 retinoic acid receptor signaling pathway GO:0051091 positive regulation of sequence-specific DNA binding transcription factor activity GO:0051271 negative regulation of cellular component movement GO:0060333 interferon-gamma-mediated signaling pathway GO:0060337 type I interferon signaling pathway GO:1902041 regulation of extrinsic apoptotic signaling pathway via death domain receptors GO:1902044 regulation of Fas signaling pathway GO:1903507 negative regulation of nucleic acid-templated transcription
US Server
Sequence and Links to Tools and Databases 
Common Gene Haplotype Alleles 
