Human Gene SRPK3 (ENST00000370101.8_9) from GENCODE V47lift37
  Description: SRSF protein kinase 3, transcript variant 1 (from RefSeq NM_014370.4)
Gencode Transcript: ENST00000370101.8_9
Gencode Gene: ENSG00000184343.11_14
Transcript (Including UTRs)
   Position: hg19 chrX:153,046,496-153,051,185 Size: 4,690 Total Exon Count: 15 Strand: +
Coding Region
   Position: hg19 chrX:153,046,542-153,050,975 Size: 4,434 Coding Exon Count: 15 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chrX:153,046,496-153,051,185)mRNA (may differ from genome)Protein (567 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMGIOMIMPubMedUniProtKBBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: SRPK3_HUMAN
DESCRIPTION: RecName: Full=SRSF protein kinase 3; EC=2.7.11.1; AltName: Full=Muscle-specific serine kinase 1; Short=MSSK-1; AltName: Full=Serine/arginine-rich protein-specific kinase 3; Short=SR-protein-specific kinase 3; AltName: Full=Serine/threonine-protein kinase 23;
FUNCTION: Serine/arginine-rich protein-specific kinase which specifically phosphorylates its substrates at serine residues located in regions rich in arginine/serine dipeptides, known as RS domains. Phosphorylates the SR splicing factor SRSF1 and the lamin-B receptor (LBR) in vitro. Required for normal muscle development (By similarity).
CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.
TISSUE SPECIFICITY: Exclusively expressed in skeletal and heart muscle.
SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family.
SIMILARITY: Contains 1 protein kinase domain.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
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-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 29.75 RPKM in Muscle - Skeletal
Total median expression: 173.36 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
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-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -22.5046-0.489 Picture PostScript Text
3' UTR -82.90210-0.395 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR011009 - Kinase-like_dom
IPR000719 - Prot_kinase_cat_dom
IPR017441 - Protein_kinase_ATP_BS
IPR008271 - Ser/Thr_kinase_AS

Pfam Domains:
PF00069 - Protein kinase domain
PF07714 - Protein tyrosine and serine/threonine kinase

SCOP Domains:
56112 - Protein kinase-like (PK-like)

ModBase Predicted Comparative 3D Structure on Q9UPE1
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000166 nucleotide binding
GO:0004672 protein kinase activity
GO:0004674 protein serine/threonine kinase activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0016301 kinase activity
GO:0016740 transferase activity

Biological Process:
GO:0000245 spliceosomal complex assembly
GO:0006468 protein phosphorylation
GO:0007275 multicellular organism development
GO:0007517 muscle organ development
GO:0007519 skeletal muscle tissue development
GO:0016310 phosphorylation
GO:0030154 cell differentiation
GO:0035556 intracellular signal transduction
GO:0050684 regulation of mRNA processing
GO:0060537 muscle tissue development

Cellular Component:
GO:0005575 cellular_component
GO:0005634 nucleus
GO:0005737 cytoplasm


-  Descriptions from all associated GenBank mRNAs
  AB209155 - Homo sapiens mRNA for serine/threonine kinase 23 variant protein.
BC117124 - Homo sapiens SFRS protein kinase 3, mRNA (cDNA clone MGC:150733 IMAGE:40125675), complete cds.
AK314828 - Homo sapiens cDNA, FLJ95709.
AK301749 - Homo sapiens cDNA FLJ58116 complete cds, highly similar to Homo sapiens serine/threonine kinase 23 (STK23), mRNA.
BC092416 - Homo sapiens SFRS protein kinase 3, mRNA (cDNA clone MGC:102944 IMAGE:30529915), complete cds.
AF027406 - Homo sapiens muscle-specific serine kinase 1 (MSSK1) mRNA, complete cds.
AB489184 - Synthetic construct DNA, clone: pF1KB5980, Homo sapiens SRPK3 gene for SFRS protein kinase 3, without stop codon, in Flexi system.
HQ258470 - Synthetic construct Homo sapiens clone IMAGE:100072899 Unknown protein gene, encodes complete protein.
KJ898635 - Synthetic construct Homo sapiens clone ccsbBroadEn_08029 SRPK3 gene, encodes complete protein.
DQ099381 - Homo sapiens serine/threonine kinase 23 mRNA, complete cds, alternatively spliced.
U82808 - Homo sapiens muscle-specific serine kinase 1 (MSSK1) mRNA, complete cds.
JD390325 - Sequence 371349 from Patent EP1572962.
JD126493 - Sequence 107517 from Patent EP1572962.
JD278248 - Sequence 259272 from Patent EP1572962.

-  Other Names for This Gene
  Alternate Gene Symbols: ENST00000370101.1, ENST00000370101.2, ENST00000370101.3, ENST00000370101.4, ENST00000370101.5, ENST00000370101.6, ENST00000370101.7, MSSK1, NM_014370, Q13583, Q4F970, Q562F5, Q9UM62, Q9UPE1, SRPK3_HUMAN, STK23, uc318ick.1, uc318ick.2
UCSC ID: ENST00000370101.8_9
RefSeq Accession: NM_014370.4
Protein: Q9UPE1 (aka SRPK3_HUMAN)

-  Gene Model Information
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-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.