Human Gene SRSF12 (ENST00000452027.3_8) from GENCODE V47lift37
  Description: serine and arginine rich splicing factor 12, transcript variant 1 (from RefSeq NM_080743.5)
Gencode Transcript: ENST00000452027.3_8
Gencode Gene: ENSG00000154548.10_10
Transcript (Including UTRs)
   Position: hg19 chr6:89,805,678-89,827,790 Size: 22,113 Total Exon Count: 5 Strand: -
Coding Region
   Position: hg19 chr6:89,808,297-89,827,606 Size: 19,310 Coding Exon Count: 5 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr6:89,805,678-89,827,790)mRNA (may differ from genome)Protein (261 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMGIPubMedUniProtKBBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: SRS12_HUMAN
DESCRIPTION: RecName: Full=Serine/arginine-rich splicing factor 12; AltName: Full=35 kDa SR repressor protein; Short=SRrp35; AltName: Full=Splicing factor, arginine/serine-rich 13B; AltName: Full=Splicing factor, arginine/serine-rich 19;
FUNCTION: Splicing factor that seems to antagonize SR proteins in pre-mRNA splicing regulation.
SUBCELLULAR LOCATION: Nucleus (Probable).
TISSUE SPECIFICITY: Expressed in testis.
SIMILARITY: Belongs to the splicing factor SR family.
SIMILARITY: Contains 1 RRM (RNA recognition motif) domain.
SEQUENCE CAUTION: Sequence=CAI16247.1; Type=Erroneous gene model prediction; Sequence=EAW48561.1; Type=Erroneous gene model prediction;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 11.90 RPKM in Testis
Total median expression: 56.40 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
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-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -99.70184-0.542 Picture PostScript Text
3' UTR -591.602619-0.226 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR012677 - Nucleotide-bd_a/b_plait
IPR000504 - RRM_dom

Pfam Domains:
PF00076 - RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain)

SCOP Domains:
54928 - RNA-binding domain, RBD

ModBase Predicted Comparative 3D Structure on Q8WXF0
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0003676 nucleic acid binding
GO:0003723 RNA binding
GO:0050733 RS domain binding
GO:0051082 unfolded protein binding

Biological Process:
GO:0000244 spliceosomal tri-snRNP complex assembly
GO:0000381 regulation of alternative mRNA splicing, via spliceosome
GO:0000395 mRNA 5'-splice site recognition
GO:0006397 mRNA processing
GO:0008380 RNA splicing
GO:0048025 negative regulation of mRNA splicing, via spliceosome

Cellular Component:
GO:0005634 nucleus
GO:0005654 nucleoplasm


-  Descriptions from all associated GenBank mRNAs
  DQ892070 - Synthetic construct clone IMAGE:100004700; FLH182868.01X; RZPDo839D06140D serine-arginine repressor protein (35 kDa) (SRrp35) gene, encodes complete protein.
DQ895263 - Synthetic construct Homo sapiens clone IMAGE:100009723; FLH182865.01L; RZPDo839D06139D serine-arginine repressor protein (35 kDa) (SRrp35) gene, encodes complete protein.
KJ900167 - Synthetic construct Homo sapiens clone ccsbBroadEn_09561 SRSF12 gene, encodes complete protein.
AK027365 - Homo sapiens cDNA FLJ14459 fis, clone HEMBB1002409.
AK090803 - Homo sapiens cDNA FLJ33484 fis, clone BRAMY2003117.
AK123215 - Homo sapiens cDNA FLJ41221 fis, clone BRAMY2028914, highly similar to 35 kDa SR repressor protein.
JD425436 - Sequence 406460 from Patent EP1572962.
JD117185 - Sequence 98209 from Patent EP1572962.
BC021715 - Homo sapiens serine-arginine repressor protein (35 kDa), mRNA (cDNA clone MGC:26758 IMAGE:4828704), complete cds.
JD234795 - Sequence 215819 from Patent EP1572962.
JD553009 - Sequence 534033 from Patent EP1572962.
AF449428 - Homo sapiens SRrp35 mRNA, complete cds.
AK314008 - Homo sapiens cDNA, FLJ94662, highly similar to Homo sapiens serine-arginine repressor protein (35 kDa) (SRrp35), mRNA.
AB527997 - Synthetic construct DNA, clone: pF1KE0673, Homo sapiens RP11-63L7.3 gene for 35 kDa SR repressor protein, without stop codon, in Flexi system.
CU692392 - Synthetic construct Homo sapiens gateway clone IMAGE:100018658 5' read SRrp35 mRNA.

-  Other Names for This Gene
  Alternate Gene Symbols: B2RA22, ENST00000452027.1, ENST00000452027.2, NM_080743, Q5T7K0, Q8WW25, Q8WXF0, SFRS13B, SFRS19, SRRP35, SRS12_HUMAN, uc320tjc.1, uc320tjc.2
UCSC ID: ENST00000452027.3_8
RefSeq Accession: NM_080743.5
Protein: Q8WXF0 (aka SRS12_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.