Human Gene ST3GAL4 (ENST00000444328.7_11) from GENCODE V47lift37
  Description: ST3 beta-galactoside alpha-2,3-sialyltransferase 4, transcript variant 2 (from RefSeq NM_001254757.2)
Gencode Transcript: ENST00000444328.7_11
Gencode Gene: ENSG00000110080.20_19
Transcript (Including UTRs)
   Position: hg19 chr11:126,225,581-126,284,533 Size: 58,953 Total Exon Count: 11 Strand: +
Coding Region
   Position: hg19 chr11:126,276,051-126,283,942 Size: 7,892 Coding Exon Count: 10 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
Gene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther Species
GO AnnotationsmRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr11:126,225,581-126,284,533)mRNA (may differ from genome)Protein (333 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMalacardsMGIOMIMPubMedReactome
UniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: SIA4C_HUMAN
DESCRIPTION: RecName: Full=CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 4; Short=Alpha 2,3-ST 4; Short=Beta-galactoside alpha-2,3-sialyltransferase 4; EC=2.4.99.-; AltName: Full=Alpha 2,3-sialyltransferase IV; AltName: Full=Gal-NAc6S; AltName: Full=Gal-beta-1,4-GalNAc-alpha-2,3-sialyltransferase; AltName: Full=SAT-3; AltName: Full=ST-4; AltName: Full=ST3Gal IV; Short=ST3GalIV; AltName: Full=ST3GalA.2; AltName: Full=STZ; AltName: Full=Sialyltransferase 4C; Short=SIAT4-C;
FUNCTION: It may catalyze the formation of the NeuAc-alpha-2,3- Gal-beta-1,3-GalNAc- or NeuAc-alpha-2,3-Gal-beta-1,3-GlcNAc- sequences found in terminal carbohydrate groups of glycoproteins and glycolipids. It may be involved in the biosynthesis of the sialyl Lewis X determinant. Also acts on the corresponding 1,3- galactosyl derivative.
CATALYTIC ACTIVITY: CMP-N-acetylneuraminate + beta-D-galactosyl- 1,4-N-acetyl-D-glucosaminyl-glycoprotein = CMP + alpha-N- acetylneuraminyl-2,3-beta-D-galactosyl-1,4-N-acetyl-D- glucosaminyl-glycoprotein.
PATHWAY: Protein modification; protein glycosylation.
SUBCELLULAR LOCATION: Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. Secreted. Note=Membrane- bound form in trans cisternae of Golgi. Secreted into the body fluid.
TISSUE SPECIFICITY: Highly expressed in adult placenta, ovary and testes.
PTM: The soluble form derives from the membrane form by proteolytic processing.
SIMILARITY: Belongs to the glycosyltransferase 29 family.
WEB RESOURCE: Name=GGDB; Note=GlycoGene database; URL="http://riodb.ibase.aist.go.jp/rcmg/ggdb/Homolog?cat=symbol&symbol=ST3GAL4";
WEB RESOURCE: Name=Functional Glycomics Gateway - GTase; Note=ST3Gal IV; URL="http://www.functionalglycomics.org/glycomics/molecule/jsp/glycoEnzyme/viewGlycoEnzyme.jsp?gbpId=gt_hum_625";

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: ST3GAL4
Diseases sorted by gene-association score: hyperglycemia (5), diabetes mellitus, insulin-dependent (4)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 38.04 RPKM in Ovary
Total median expression: 493.86 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -108.30217-0.499 Picture PostScript Text
3' UTR -254.70591-0.431 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR001675 - Glyco_trans_29
IPR012163 - Sialyl_trans

Pfam Domains:
PF00777 - Glycosyltransferase family 29 (sialyltransferase)

ModBase Predicted Comparative 3D Structure on Q11206
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
Gene SorterGene Sorter    
 RGD    
      
      

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0003836 beta-galactoside (CMP) alpha-2,3-sialyltransferase activity
GO:0008373 sialyltransferase activity
GO:0016740 transferase activity
GO:0016757 transferase activity, transferring glycosyl groups
GO:0047288 monosialoganglioside sialyltransferase activity

Biological Process:
GO:0006486 protein glycosylation
GO:0009101 glycoprotein biosynthetic process
GO:0009247 glycolipid biosynthetic process
GO:0009311 oligosaccharide metabolic process
GO:0009312 oligosaccharide biosynthetic process
GO:0016266 O-glycan processing
GO:0018146 keratan sulfate biosynthetic process
GO:0018279 protein N-linked glycosylation via asparagine
GO:0030259 lipid glycosylation
GO:0050890 cognition
GO:0097503 sialylation
GO:1990743 protein sialylation

Cellular Component:
GO:0000139 Golgi membrane
GO:0005576 extracellular region
GO:0005794 Golgi apparatus
GO:0016020 membrane
GO:0016021 integral component of membrane
GO:0032580 Golgi cisterna membrane


-  Descriptions from all associated GenBank mRNAs
  E06059 - cDNA encoding alpha 2 to 3 sialyltransferase.
LF384537 - JP 2014500723-A/192040: Polycomb-Associated Non-Coding RNAs.
X74570 - H.sapiens mRNA for Gal-beta(1-3/1-4)GlcNAc alpha-2.3-sialyltransferase.
BC010645 - Homo sapiens ST3 beta-galactoside alpha-2,3-sialyltransferase 4, mRNA (cDNA clone MGC:5017 IMAGE:3449625), complete cds.
AK314738 - Homo sapiens cDNA, FLJ95600, Homo sapiens sialyltransferase 4C (beta-galactosidealpha-2,3-sialyltransferase) (SIAT4C), mRNA.
AK291577 - Homo sapiens cDNA FLJ75432 complete cds, highly similar to Homo sapiens alpha 2,3-sialyltransferase IV type B+18 mRNA.
JD303883 - Sequence 284907 from Patent EP1572962.
JD074782 - Sequence 55806 from Patent EP1572962.
JD371637 - Sequence 352661 from Patent EP1572962.
KJ901738 - Synthetic construct Homo sapiens clone ccsbBroadEn_11132 ST3GAL4 gene, encodes complete protein.
EU831879 - Synthetic construct Homo sapiens clone HAIB:100066908; DKFZo004E1022 ST3 beta-galactoside alpha-2,3-sialyltransferase 4 protein (ST3GAL4) gene, encodes complete protein.
EU831802 - Synthetic construct Homo sapiens clone HAIB:100066831; DKFZo008E1021 ST3 beta-galactoside alpha-2,3-sialyltransferase 4 protein (ST3GAL4) gene, encodes complete protein.
CR456858 - Homo sapiens full open reading frame cDNA clone RZPDo834B1211D for gene SIAT4C, sialyltransferase 4C (beta-galactoside alpha-2,3-sialyltransferase); complete cds, incl. stopcodon.
AY040826 - Homo sapiens alpha 2,3-sialyltransferase IV type B+18 mRNA, complete cds; alternatively spliced.
AF516604 - Homo sapiens alpha 2,3-sialyltransferase IV type A1-12 mRNA, complete cds; alternatively spliced.
AF525084 - Homo sapiens alpha-2,3-sialyltransferase IV type A1+18 mRNA, complete cds; alternatively spliced.
L23767 - Human a2,3 sialyltransferse mRNa, complete cds.
AF516602 - Homo sapiens alpha 2,3-sialyltransferase IV type A2+18 mRNA, complete cds; alternatively spliced.
AF516603 - Homo sapiens alpha 2,3 sialyltransferase IV type A2-12 mRNA, complete cds; alternatively spliced.
AK021929 - Homo sapiens cDNA FLJ11867 fis, clone HEMBA1006976, weakly similar to H.sapiens mRNA for Gal-beta(1-3/1-4)GlcNAc alpha-2.3-sialyltransferase.
LF375988 - JP 2014500723-A/183491: Polycomb-Associated Non-Coding RNAs.
AF035249 - Homo sapiens CMP-N-acetylneuraminate-beta-galactosamide-alpha-2, 3-sialyltransferase mRNA, partial cds.
LF375986 - JP 2014500723-A/183489: Polycomb-Associated Non-Coding RNAs.
LF375985 - JP 2014500723-A/183488: Polycomb-Associated Non-Coding RNAs.
LF375984 - JP 2014500723-A/183487: Polycomb-Associated Non-Coding RNAs.
AK128605 - Homo sapiens cDNA FLJ46764 fis, clone TRACH3024428.
LF375978 - JP 2014500723-A/183481: Polycomb-Associated Non-Coding RNAs.
JD061874 - Sequence 42898 from Patent EP1572962.
JD170015 - Sequence 151039 from Patent EP1572962.
JD444639 - Sequence 425663 from Patent EP1572962.
JD142722 - Sequence 123746 from Patent EP1572962.
JD546369 - Sequence 527393 from Patent EP1572962.
JD320774 - Sequence 301798 from Patent EP1572962.
JD119461 - Sequence 100485 from Patent EP1572962.
JD140891 - Sequence 121915 from Patent EP1572962.
LF375977 - JP 2014500723-A/183480: Polycomb-Associated Non-Coding RNAs.
JD220235 - Sequence 201259 from Patent EP1572962.
MA620114 - JP 2018138019-A/192040: Polycomb-Associated Non-Coding RNAs.
MA611565 - JP 2018138019-A/183491: Polycomb-Associated Non-Coding RNAs.
MA611563 - JP 2018138019-A/183489: Polycomb-Associated Non-Coding RNAs.
MA611562 - JP 2018138019-A/183488: Polycomb-Associated Non-Coding RNAs.
MA611561 - JP 2018138019-A/183487: Polycomb-Associated Non-Coding RNAs.
MA611555 - JP 2018138019-A/183481: Polycomb-Associated Non-Coding RNAs.
MA611554 - JP 2018138019-A/183480: Polycomb-Associated Non-Coding RNAs.

-  Biochemical and Signaling Pathways
  BioCyc Knowledge Library
PWY-7434 - terminal O-glycans residues modification (via type 2 precursor disaccharide)
PWY-7831 - ABH and Lewis epitopes biosynthesis from type 2 precursor disaccharide
PWY-7835 - superpathway of glycosphingolipids biosynthesis
PWY-7841 - neolacto-series glycosphingolipids biosynthesis

BioCarta from NCI Cancer Genome Anatomy Project
h_aMANpathway - Steps in the Glycosylation of Mammalian N-linked Oligosaccarides

Reactome (by CSHL, EBI, and GO)

Protein Q11206 (Reactome details) participates in the following event(s):

R-HSA-1022129 ST3GAL4 transfers Neu5Ac to terminal Gal of N-glycans
R-HSA-981497 ST3GAL1-4 can add a sialic acid to the T antigen at the alpha 3 position
R-HSA-2046285 The keratan chain can be capped by N-acetylneuraminic acid
R-HSA-4084984 ST3GAL1-6 transfer Neu5Ac to terminal Gal (alpha-2,3 link)
R-HSA-1912378 Sialylation of Pre-NOTCH
R-HSA-975577 N-Glycan antennae elongation
R-HSA-977068 Termination of O-glycan biosynthesis
R-HSA-2022854 Keratan sulfate biosynthesis
R-HSA-4085001 Sialic acid metabolism
R-HSA-1912420 Pre-NOTCH Processing in Golgi
R-HSA-975576 N-glycan antennae elongation in the medial/trans-Golgi
R-HSA-913709 O-linked glycosylation of mucins
R-HSA-1638074 Keratan sulfate/keratin metabolism
R-HSA-446219 Synthesis of substrates in N-glycan biosythesis
R-HSA-1912422 Pre-NOTCH Expression and Processing
R-HSA-948021 Transport to the Golgi and subsequent modification
R-HSA-5173105 O-linked glycosylation
R-HSA-1630316 Glycosaminoglycan metabolism
R-HSA-446193 Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein
R-HSA-157118 Signaling by NOTCH
R-HSA-446203 Asparagine N-linked glycosylation
R-HSA-597592 Post-translational protein modification
R-HSA-71387 Metabolism of carbohydrates
R-HSA-162582 Signal Transduction
R-HSA-392499 Metabolism of proteins
R-HSA-1430728 Metabolism

-  Other Names for This Gene
  Alternate Gene Symbols: A8K6B2, CGS23, ENST00000444328.1, ENST00000444328.2, ENST00000444328.3, ENST00000444328.4, ENST00000444328.5, ENST00000444328.6, NANTA3, NM_001254757, O60497, Q11206, Q8N6A6, Q8NFG7, Q96QQ9, SIA4C_HUMAN, SIAT4C, STZ, uc320mes.1, uc320mes.2
UCSC ID: ENST00000444328.7_11
RefSeq Accession: NM_001254757.2
Protein: Q11206 (aka SIA4C_HUMAN or SI4C_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.