Human Gene STK25 (ENST00000316586.9_7) from GENCODE V47lift37
  Description: serine/threonine kinase 25, transcript variant 4 (from RefSeq NM_006374.5)
Gencode Transcript: ENST00000316586.9_7
Gencode Gene: ENSG00000115694.15_15
Transcript (Including UTRs)
   Position: hg19 chr2:242,432,085-242,447,999 Size: 15,915 Total Exon Count: 12 Strand: -
Coding Region
   Position: hg19 chr2:242,435,077-242,447,450 Size: 12,374 Coding Exon Count: 11 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
Gene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther Species
GO AnnotationsmRNA DescriptionsOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr2:242,432,085-242,447,999)mRNA (may differ from genome)Protein (426 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
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HGNCMalacardsMGIOMIMPubMedUniProtKB
WikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: STK25_HUMAN
DESCRIPTION: RecName: Full=Serine/threonine-protein kinase 25; EC=2.7.11.1; AltName: Full=Ste20-like kinase; AltName: Full=Sterile 20/oxidant stress-response kinase 1; Short=SOK-1; Short=Ste20/oxidant stress response kinase 1;
FUNCTION: Oxidant stress-activated serine/threonine kinase that may play a role in the response to environmental stress. Targets to the Golgi apparatus where it appears to regulate protein transport events, cell adhesion, and polarity complexes important for cell migration.
CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.
COFACTOR: Magnesium.
ENZYME REGULATION: Interaction with Golgi matrix protein GOLGA2 leads to autophosphorylation on Thr-174, possibly as a consequence of stabilization of dimer formation. The C-terminal non-catalytic region inhibits the kinase activity.
SUBUNIT: Homodimer.
INTERACTION: Q62839:Golga2 (xeno); NbExp=2; IntAct=EBI-618295, EBI-618335; Q9BUL8:PDCD10; NbExp=7; IntAct=EBI-618295, EBI-740195; O43815:STRN; NbExp=2; IntAct=EBI-618295, EBI-1046642; P63104:YWHAZ; NbExp=2; IntAct=EBI-618295, EBI-347088;
SUBCELLULAR LOCATION: Cytoplasm. Golgi apparatus. Note=Localizes to the Golgi apparatus.
TISSUE SPECIFICITY: Ubiquitously expressed. Highest levels are found in testis, large intestine, brain and stomach followed by heart and lung.
PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.
SIMILARITY: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
SIMILARITY: Contains 1 protein kinase domain.
WEB RESOURCE: Name=NIEHS-SNPs; URL="http://egp.gs.washington.edu/data/stk25/";

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: STK25
Diseases sorted by gene-association score: cerebral cavernous malformations-2 (2)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 54.82 RPKM in Muscle - Skeletal
Total median expression: 1264.59 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -140.50242-0.581 Picture PostScript Text
3' UTR -1215.502992-0.406 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR011009 - Kinase-like_dom
IPR000719 - Prot_kinase_cat_dom
IPR017441 - Protein_kinase_ATP_BS
IPR002290 - Ser/Thr_dual-sp_kinase_dom

Pfam Domains:
PF00069 - Protein kinase domain
PF07714 - Protein tyrosine and serine/threonine kinase

SCOP Domains:
56112 - Protein kinase-like (PK-like)

Protein Data Bank (PDB) 3-D Structure
MuPIT help
2XIK - X-ray MuPIT


ModBase Predicted Comparative 3D Structure on O00506
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000166 nucleotide binding
GO:0004672 protein kinase activity
GO:0004674 protein serine/threonine kinase activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0008349 MAP kinase kinase kinase kinase activity
GO:0016301 kinase activity
GO:0016740 transferase activity
GO:0042803 protein homodimerization activity
GO:0046872 metal ion binding

Biological Process:
GO:0000165 MAPK cascade
GO:0000185 activation of MAPKKK activity
GO:0006468 protein phosphorylation
GO:0006979 response to oxidative stress
GO:0007163 establishment or maintenance of cell polarity
GO:0007165 signal transduction
GO:0007346 regulation of mitotic cell cycle
GO:0016310 phosphorylation
GO:0032874 positive regulation of stress-activated MAPK cascade
GO:0036481 intrinsic apoptotic signaling pathway in response to hydrogen peroxide
GO:0042542 response to hydrogen peroxide
GO:0042981 regulation of apoptotic process
GO:0046777 protein autophosphorylation
GO:0048812 neuron projection morphogenesis
GO:0050772 positive regulation of axonogenesis
GO:0051645 Golgi localization
GO:0051683 establishment of Golgi localization
GO:0090168 Golgi reassembly

Cellular Component:
GO:0000139 Golgi membrane
GO:0005737 cytoplasm
GO:0005794 Golgi apparatus
GO:0070062 extracellular exosome


-  Descriptions from all associated GenBank mRNAs
  AK124871 - Homo sapiens cDNA FLJ42881 fis, clone BRHIP3004968.
JD472370 - Sequence 453394 from Patent EP1572962.
JD135981 - Sequence 117005 from Patent EP1572962.
JD135980 - Sequence 117004 from Patent EP1572962.
JD181008 - Sequence 162032 from Patent EP1572962.
JD135979 - Sequence 117003 from Patent EP1572962.
JD381889 - Sequence 362913 from Patent EP1572962.
CR936874 - Homo sapiens mRNA; cDNA DKFZp686J1430 (from clone DKFZp686J1430).
BC007852 - Homo sapiens serine/threonine kinase 25 (STE20 homolog, yeast), mRNA (cDNA clone MGC:14329 IMAGE:4298239), complete cds.
JD189926 - Sequence 170950 from Patent EP1572962.
BC015793 - Homo sapiens serine/threonine kinase 25 (STE20 homolog, yeast), mRNA (cDNA clone MGC:23674 IMAGE:4867978), complete cds.
BC091505 - Homo sapiens serine/threonine kinase 25 (STE20 homolog, yeast), mRNA (cDNA clone MGC:111108 IMAGE:30383354), complete cds.
D63780 - Homo sapiens YSK1 mRNA for protein kinase, complete cds.
AK092161 - Homo sapiens cDNA FLJ34842 fis, clone NT2NE2011033, highly similar to Serine/threonine-protein kinase 25 (EC 2.7.11.1).
X99325 - H.sapiens mRNA for Ste20-like kinase.
JD274040 - Sequence 255064 from Patent EP1572962.
JD355533 - Sequence 336557 from Patent EP1572962.
JD276399 - Sequence 257423 from Patent EP1572962.
AK291808 - Homo sapiens cDNA FLJ78718 complete cds.
AK303719 - Homo sapiens cDNA FLJ61527 complete cds, highly similar to Serine/threonine-protein kinase 25 (EC 2.7.11.1).
JD261030 - Sequence 242054 from Patent EP1572962.
JD483275 - Sequence 464299 from Patent EP1572962.
JD185199 - Sequence 166223 from Patent EP1572962.
JD176659 - Sequence 157683 from Patent EP1572962.
JD406477 - Sequence 387501 from Patent EP1572962.
JD418005 - Sequence 399029 from Patent EP1572962.
JD549954 - Sequence 530978 from Patent EP1572962.
AK315966 - Homo sapiens cDNA, FLJ78865 complete cds, highly similar to Serine/threonine-protein kinase 25 (EC 2.7.11.1).
JD078515 - Sequence 59539 from Patent EP1572962.
JD216974 - Sequence 197998 from Patent EP1572962.
JD206864 - Sequence 187888 from Patent EP1572962.
AK301214 - Homo sapiens cDNA FLJ52778 complete cds, highly similar to Serine/threonine-protein kinase 25 (EC 2.7.11.1).
AK297374 - Homo sapiens cDNA FLJ59346 complete cds, highly similar to Serine/threonine-protein kinase 25 (EC 2.7.11.1).
AK291947 - Homo sapiens cDNA FLJ75758 complete cds, highly similar to Homo sapiens serine/threonine kinase 25 (STE20 homolog, yeast) (STK25), mRNA.
JD434353 - Sequence 415377 from Patent EP1572962.
KJ893064 - Synthetic construct Homo sapiens clone ccsbBroadEn_02458 STK25 gene, encodes complete protein.
KJ905395 - Synthetic construct Homo sapiens clone ccsbBroadEn_14966 STK25 gene, encodes complete protein.
BT019961 - Homo sapiens serine/threonine kinase 25 (STE20 homolog, yeast) mRNA, complete cds.
KJ906045 - Synthetic construct Homo sapiens clone ccsbBroadEn_15715 STK25 gene, encodes complete protein.
DQ893230 - Synthetic construct clone IMAGE:100005860; FLH195438.01X; RZPDo839C05152D serine/threonine kinase 25 (STE20 homolog, yeast) (STK25) gene, encodes complete protein.
DQ896559 - Synthetic construct Homo sapiens clone IMAGE:100011019; FLH195434.01L; RZPDo839C05151D serine/threonine kinase 25 (STE20 homolog, yeast) (STK25) gene, encodes complete protein.
KU178528 - Homo sapiens serine/threonine kinase 25 isoform 1 (STK25) mRNA, partial cds.
KU178529 - Homo sapiens serine/threonine kinase 25 isoform 2 (STK25) mRNA, complete cds, alternatively spliced.
KU178530 - Homo sapiens serine/threonine kinase 25 isoform 3 (STK25) mRNA, partial cds, alternatively spliced.
AB529004 - Synthetic construct DNA, clone: pF1KE0948, Homo sapiens STK25 gene for serine/threonine kinase 25, without stop codon, in Flexi system.
CU677445 - Synthetic construct Homo sapiens gateway clone IMAGE:100017244 5' read STK25 mRNA.
AK302751 - Homo sapiens cDNA FLJ58867 complete cds, highly similar to Serine/threonine-protein kinase 25 (EC 2.7.11.1).
CU675833 - Synthetic construct Homo sapiens gateway clone IMAGE:100019344 5' read STK25 mRNA.
JD409865 - Sequence 390889 from Patent EP1572962.

-  Other Names for This Gene
  Alternate Gene Symbols: A8K6Z3, A8K7D2, B7Z9K1, ENST00000316586.1, ENST00000316586.2, ENST00000316586.3, ENST00000316586.4, ENST00000316586.5, ENST00000316586.6, ENST00000316586.7, ENST00000316586.8, NM_006374, O00506, Q15522, Q5BJF1, SOK1, STK25_HUMAN, uc317qba.1, uc317qba.2, YSK1
UCSC ID: ENST00000316586.9_7
RefSeq Accession: NM_001271977.2
Protein: O00506 (aka STK25_HUMAN or ST25_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.