ID:SYMPK_HUMAN DESCRIPTION: RecName: Full=Symplekin; FUNCTION: Heat-labile component of a multimolecular complex that function in histone mRNA 3'-end processing. Specific component of the tight junction (TJ) plaque, but might not be an exclusively junctional component. May have a house-keeping rule. May be required for pre-mRNA polyadenylation. SUBUNIT: Found in a heat-sensitive complex at least composed of several cleavage and polyadenylation specific and cleavage stimulation factors. Interacts with CPSF2, CPSF3 and CSTF2. Interacts with HSF1 in heat-stressed cells. SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton. Cell junction, tight junction. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction. Nucleus, nucleoplasm. Note=Cytoplasmic face of adhesion plaques (major) and nucleoplasm (minor) (in cells with TJ). Nucleoplasm (in cells without TJ). Nuclear bodies of heat-stressed cells. TISSUE SPECIFICITY: In testis, expressed in polar epithelia and Sertoli cells but not in vascular endothelia. The protein is detected in stomach, duodenum, pancreas, liver, fetal brain, carcinomas, lens-forming cells, fibroblasts, lymphocytes, lymphoma cells, erythroleukemia cells but not in endothelium of vessels, epidermis, intercalated disks, Purkinje fiber cells of the heart and lymph node. MISCELLANEOUS: Could be used as a differentiation marker in the differential diagnosis of tumors. SEQUENCE CAUTION: Sequence=AAC50667.1; Type=Erroneous initiation; Note=Translation N-terminally extended; Sequence=AAH30214.1; Type=Miscellaneous discrepancy; Note=Contaminating sequence. Potential poly-A sequence; Sequence=BAD92261.1; Type=Erroneous initiation; Note=Translation N-terminally shortened; Sequence=CAA71861.1; Type=Frameshift; Positions=67;
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on Q92797
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
AK130602 - Homo sapiens cDNA FLJ27092 fis, clone SPL04282, highly similar to Symplekin. AB209024 - Homo sapiens mRNA for SYMPK protein variant protein. BX649165 - Homo sapiens mRNA; cDNA DKFZp686J1348 (from clone DKFZp686J1348). U49240 - Human symplekin mRNA, complete cds. BC006536 - Homo sapiens symplekin, mRNA (cDNA clone IMAGE:2963685), partial cds. BC006567 - Homo sapiens symplekin, mRNA (cDNA clone IMAGE:2963685), partial cds. AK300252 - Homo sapiens cDNA FLJ61705 complete cds, highly similar to Symplekin. Y10931 - H.sapiens mRNA for symplekin. JD485314 - Sequence 466338 from Patent EP1572962. JD257217 - Sequence 238241 from Patent EP1572962. JD202986 - Sequence 184010 from Patent EP1572962. JD218782 - Sequence 199806 from Patent EP1572962. DQ893447 - Synthetic construct clone IMAGE:100006077; FLH199538.01X; RZPDo839H1182D symplekin (SYMPK) gene, encodes complete protein. EU176761 - Synthetic construct Homo sapiens clone IMAGE:100011608; FLH263878.01L; RZPDo839A05258D symplekin (SYMPK) gene, encodes complete protein. AB210010 - Homo sapiens mRNA for SYMPK variant protein, partial cds, clone: fh12484. AB527248 - Synthetic construct DNA, clone: pF1KB0026, Homo sapiens SYMPK gene for symplekin, without stop codon, in Flexi system. BC030214 - Homo sapiens symplekin, mRNA (cDNA clone IMAGE:5202004), complete cds. U88726 - Homo sapiens symplekin mRNA, partial cds. JD212133 - Sequence 193157 from Patent EP1572962. AF049526 - Homo sapiens symplekin mRNA, partial cds. JD291913 - Sequence 272937 from Patent EP1572962. JD267376 - Sequence 248400 from Patent EP1572962.
Biochemical and Signaling Pathways
Reactome (by CSHL, EBI, and GO)
Protein Q92797 (Reactome details) participates in the following event(s):
R-HSA-72231 Cleavage and Polyadenylation R-HSA-77589 Recognition of AAUAAA sequence by CPSF R-HSA-72185 mRNA polyadenylation R-HSA-77594 Docking of the Mature intronless derived transcript derived mRNA, TAP and Aly/Ref at the NPC R-HSA-72130 Formation of an intermediate Spliceosomal C (Bact) complex R-HSA-72180 Cleavage of mRNA at the 3'-end R-HSA-77590 Recruitment of CstF to the CPSF Bound Pre-mRNA R-HSA-77592 Cleavage of Intronless Pre-mRNA at 3'-end R-HSA-77593 Cleavage and polyadenylation of Intronless Pre-mRNA R-HSA-72143 Lariat Formation and 5'-Splice Site Cleavage R-HSA-72139 Formation of the active Spliceosomal C (B*) complex R-HSA-8849157 TREX complex binds spliced, capped mRNA:CBC:EJC cotranscriptionally R-HSA-156661 Formation of Exon Junction Complex R-HSA-77591 Binding of Cleavage factors and Poly(A)Polymerase to the CstF:CPSF:Pre-mRNA Complex R-HSA-72163 mRNA Splicing - Major Pathway R-HSA-77595 Processing of Intronless Pre-mRNAs R-HSA-72187 mRNA 3'-end processing R-HSA-159231 Transport of Mature mRNA Derived from an Intronless Transcript R-HSA-109688 Cleavage of Growing Transcript in the Termination Region R-HSA-72172 mRNA Splicing R-HSA-75067 Processing of Capped Intronless Pre-mRNA R-HSA-72203 Processing of Capped Intron-Containing Pre-mRNA R-HSA-159234 Transport of Mature mRNAs Derived from Intronless Transcripts R-HSA-73856 RNA Polymerase II Transcription Termination R-HSA-8953854 Metabolism of RNA R-HSA-72202 Transport of Mature Transcript to Cytoplasm R-HSA-73857 RNA Polymerase II Transcription R-HSA-74160 Gene expression (Transcription)
US Server
Sequence and Links to Tools and Databases 
Common Gene Haplotype Alleles 
