ID:TAF1_HUMAN DESCRIPTION: RecName: Full=Transcription initiation factor TFIID subunit 1; EC=2.7.11.1; AltName: Full=Cell cycle gene 1 protein; AltName: Full=TBP-associated factor 250 kDa; Short=p250; AltName: Full=Transcription initiation factor TFIID 250 kDa subunit; Short=TAF(II)250; Short=TAFII-250; Short=TAFII250; FUNCTION: Largest component and core scaffold of the TFIID basal transcription factor complex. Contains novel N- and C-terminal Ser/Thr kinase domains which can autophosphorylate or transphosphorylate other transcription factors. Phosphorylates TP53 on 'Thr-55' which leads to MDM2-mediated degradation of TP53. Phosphorylates GTF2A1 and GTF2F1 on Ser residues. Possesses DNA- binding activity. Essential for progression of the G1 phase of the cell cycle. CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein. COFACTOR: Magnesium. ENZYME REGULATION: Autophosphorylates on Ser residues. Inhibited by retinoblastoma tumor suppressor protein, RB1. SUBUNIT: TAF1 is the largest component of transcription factor TFIID that is composed of TBP and a variety of TBP-associated factors. TAF1, when part of the TFIID complex, interacts with C- terminus of TP53. Component of some MLL1/MLL complex, at least composed of the core components MLL, ASH2L, HCFC1/HCF1, WDR5 and RBBP5, as well as the facultative components C17orf49, CHD8, E2F6, HSP70, INO80C, KANSL1, LAS1L, MAX, MCRS1, MGA, KAT8/MOF, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. RB1 interacts with the N- terminal domain of TAF1. Interacts with ASF1A and ASF1B. Interacts with SV40 Large T antigen. Interacts (via bromo domains) with acetylated lysine residues on the N-terminus of histone H1.4, H2A, H2B, H3 and H4 (in vitro). INTERACTION: P35269:GTF2F1; NbExp=3; IntAct=EBI-491289, EBI-457886; P20226:TBP; NbExp=5; IntAct=EBI-491289, EBI-355371; SUBCELLULAR LOCATION: Nucleus. PTM: Phosphorylated by casein kinase II in vitro. DISEASE: Defects in TAF1 are the cause of dystonia type 3 (DYT3) [MIM:314250]; also called X-linked dystonia-parkinsonism (XDP). DYT3 is a X-linked dystonia-parkinsonism disorder. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. DYT3 is characterized by severe progressive torsion dystonia followed by parkinsonism. Its prevalence is high in the Philippines. DYT3 has a well-defined pathology of extensive neuronal loss and mosaic gliosis in the striatum (caudate nucleus and putamen) which appears to resemble that in Huntington disease. SIMILARITY: Belongs to the TAF1 family. SIMILARITY: Contains 2 bromo domains. SIMILARITY: Contains 1 HMG box DNA-binding domain. SIMILARITY: Contains 2 protein kinase domains. WEB RESOURCE: Name=NIEHS-SNPs; URL="http://egp.gs.washington.edu/data/taf1/";
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on P21675
Front
Top
Side
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.
Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
Gene Ontology (GO) Annotations with Structured Vocabulary
Molecular Function: GO:0000166 nucleotide binding GO:0000979 RNA polymerase II core promoter sequence-specific DNA binding GO:0000981 RNA polymerase II transcription factor activity, sequence-specific DNA binding GO:0001129 RNA polymerase II transcription factor activity, TBP-class protein binding, involved in preinitiation complex assembly GO:0002039 p53 binding GO:0003677 DNA binding GO:0003713 transcription coactivator activity GO:0004402 histone acetyltransferase activity GO:0004674 protein serine/threonine kinase activity GO:0005515 protein binding GO:0005524 ATP binding GO:0008134 transcription factor binding GO:0016301 kinase activity GO:0016740 transferase activity GO:0016746 transferase activity, transferring acyl groups GO:0017025 TBP-class protein binding GO:0043565 sequence-specific DNA binding GO:0046982 protein heterodimerization activity GO:0061629 RNA polymerase II sequence-specific DNA binding transcription factor binding GO:0061631 ubiquitin conjugating enzyme activity GO:0070577 lysine-acetylated histone binding GO:0001075 transcription factor activity, RNA polymerase II core promoter sequence-specific binding involved in preinitiation complex assembly GO:0044212 transcription regulatory region DNA binding GO:0061628 H3K27me3 modified histone binding GO:1905502 acetyl-CoA binding
Biological Process: GO:0000209 protein polyubiquitination GO:0006351 transcription, DNA-templated GO:0006352 DNA-templated transcription, initiation GO:0006355 regulation of transcription, DNA-templated GO:0006366 transcription from RNA polymerase II promoter GO:0006367 transcription initiation from RNA polymerase II promoter GO:0006468 protein phosphorylation GO:0006511 ubiquitin-dependent protein catabolic process GO:0006974 cellular response to DNA damage stimulus GO:0007049 cell cycle GO:0010629 negative regulation of gene expression GO:0010768 negative regulation of transcription from RNA polymerase II promoter in response to UV-induced DNA damage GO:0016032 viral process GO:0016310 phosphorylation GO:0016573 histone acetylation GO:0018105 peptidyl-serine phosphorylation GO:0018107 peptidyl-threonine phosphorylation GO:0030901 midbrain development GO:0032092 positive regulation of protein binding GO:0032436 positive regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0034644 cellular response to UV GO:0036369 transcription factor catabolic process GO:0043433 negative regulation of sequence-specific DNA binding transcription factor activity GO:0045943 positive regulation of transcription from RNA polymerase I promoter GO:0045944 positive regulation of transcription from RNA polymerase II promoter GO:0046777 protein autophosphorylation GO:0050821 protein stabilization GO:0051123 RNA polymerase II transcriptional preinitiation complex assembly GO:0060260 regulation of transcription initiation from RNA polymerase II promoter GO:0060261 positive regulation of transcription initiation from RNA polymerase II promoter GO:0071318 cellular response to ATP GO:1901796 regulation of signal transduction by p53 class mediator GO:1902806 regulation of cell cycle G1/S phase transition GO:1903026 negative regulation of RNA polymerase II regulatory region sequence-specific DNA binding GO:1905524 negative regulation of protein autoubiquitination GO:2000059 negative regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process GO:2000825 positive regulation of androgen receptor activity
BioCarta from NCI Cancer Genome Anatomy Project h_p53hypoxiaPathway - Hypoxia and p53 in the Cardiovascular system
Reactome (by CSHL, EBI, and GO)
Protein P21675 (Reactome details) participates in the following event(s):
R-HSA-109636 Recognition and Binding of Core Promoter Elements by TFIID R-HSA-73946 Abortive initiation R-HSA-75856 Abortive Initiation Before Second Transition R-HSA-75873 Addition of Nucleotides 5 through 9 on the growing Transcript R-HSA-167136 Addition of nucleotides 5 through 9 on the growing HIV-1 transcript R-HSA-167474 Abortive HIV-1 Initiation Before Second Transition R-HSA-167477 Abortive HIV-1 initiation after formation of the first phosphodiester bond R-HSA-75866 Nucleophillic Attack by 3'-hydroxyl Oxygen of nascent transcript on the Alpha Phosphate of NTP R-HSA-75861 NTP Binds Active Site of RNA Polymerase II R-HSA-75864 Newly Formed Phosphodiester Bond Stabilized and PPi Released R-HSA-109637 Binding of TFIIA and TFIIB to the pol II promoter:TFIID complex R-HSA-75862 Fall Back to Closed Pre-initiation Complex R-HSA-75949 RNA Polymerase II Promoter Opening: First Transition R-HSA-167134 Newly formed phosphodiester bond stabilized and PPi released R-HSA-167130 Nucleophillic attack by 3'-hydroxyl oxygen of nascent HIV-1 transcript on the Alpha phosphate of NTP R-HSA-167118 NTP binds active site of RNA Polymerase II in HIV-1 open pre-initiation complex R-HSA-167484 Fall Back to Closed Pre-initiation Complex R-HSA-167097 HIV Promoter Opening: First Transition R-HSA-6805399 TAF1 phosphorylates TP53 R-HSA-109638 Recruitment of RNA Polymerase II Holoenzyme by TFIIF to the pol II promoter:TFIID:TFIIA:TFIIB complex R-HSA-75095 Binding of TFIIE to the growing preinitiation complex R-HSA-109639 Formation of the closed pre-initiation complex R-HSA-73779 RNA Polymerase II Transcription Pre-Initiation And Promoter Opening R-HSA-674695 RNA Polymerase II Pre-transcription Events R-HSA-73776 RNA Polymerase II Promoter Escape R-HSA-167162 RNA Polymerase II HIV Promoter Escape R-HSA-167161 HIV Transcription Initiation R-HSA-75953 RNA Polymerase II Transcription Initiation R-HSA-76042 RNA Polymerase II Transcription Initiation And Promoter Clearance R-HSA-167172 Transcription of the HIV genome R-HSA-73857 RNA Polymerase II Transcription R-HSA-6804756 Regulation of TP53 Activity through Phosphorylation R-HSA-162599 Late Phase of HIV Life Cycle R-HSA-74160 Gene expression (Transcription) R-HSA-5633007 Regulation of TP53 Activity R-HSA-162587 HIV Life Cycle R-HSA-3700989 Transcriptional Regulation by TP53 R-HSA-162906 HIV Infection R-HSA-212436 Generic Transcription Pathway R-HSA-5663205 Infectious disease R-HSA-1643685 Disease
US Server
Sequence and Links to Tools and Databases 
Common Gene Haplotype Alleles 
