ID:TAF12_HUMAN DESCRIPTION: RecName: Full=Transcription initiation factor TFIID subunit 12; AltName: Full=Transcription initiation factor TFIID 20/15 kDa subunits; Short=TAFII-20/TAFII-15; Short=TAFII20/TAFII15; FUNCTION: TAFs are components of the transcription factor IID (TFIID) complex, PCAF histone acetylase complex and TBP-free TAFII complex (TFTC). TAFs components-TIIFD are essential for mediating regulation of RNA polymerase transcription. SUBUNIT: TFIID and PCAF are composed of TATA binding protein (TBP) and a number of TBP-associated factors (TAFs). TBP is not part of TFTC. Interacts directly with TBP; additional interactions between TAFII20 and TAFII28 or TAFII30 were detected. Component of the PCAF complex, at least composed of TADA2L/ADA2, TADA3L/ADA3, TAF5L/PAF65-beta, SUPT3H, TAF6L, TAF9, TAF10, TAF12 and TRRAP. Component of the STAGA transcription coactivator-HAT complex, at least composed of SUPT3H, GCN5L2, TAF5L, TAF6L, STAF65- gamma/KIAA0764, TADA3L, TAD1L, TAF10, TAF12, TRRAP and TAF9. Interacts (isoform TAFII15 and isoform TAFII20) with ATF7 (via the transactivation domain); the interaction is prevented by sumoylation of ATF7 and promotes (isoform TAFII20 only) the transactivation of ATF7. SUBCELLULAR LOCATION: Nucleus. TISSUE SPECIFICITY: Ubiquitous. SIMILARITY: Belongs to the TAF12 family. SIMILARITY: Contains 1 histone-fold domain. WEB RESOURCE: Name=NIEHS-SNPs; URL="http://egp.gs.washington.edu/data/taf12/";
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on Q16514
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
Gene Ontology (GO) Annotations with Structured Vocabulary
Molecular Function: GO:0001102 RNA polymerase II activating transcription factor binding GO:0003677 DNA binding GO:0003713 transcription coactivator activity GO:0005515 protein binding GO:0008134 transcription factor binding GO:0017025 TBP-class protein binding GO:0046982 protein heterodimerization activity GO:0003700 transcription factor activity, sequence-specific DNA binding GO:0004402 histone acetyltransferase activity
Biological Process: GO:0006351 transcription, DNA-templated GO:0006352 DNA-templated transcription, initiation GO:0006355 regulation of transcription, DNA-templated GO:0006366 transcription from RNA polymerase II promoter GO:0006367 transcription initiation from RNA polymerase II promoter GO:0043966 histone H3 acetylation GO:0051091 positive regulation of sequence-specific DNA binding transcription factor activity GO:0051123 RNA polymerase II transcriptional preinitiation complex assembly GO:1901796 regulation of signal transduction by p53 class mediator
U57693 - Human TFIID subunits TAF20 and TAF15 mRNA, complete cds. BC011986 - Homo sapiens TAF12 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 20kDa, mRNA (cDNA clone MGC:9172 IMAGE:3882343), complete cds. X84002 - H.sapiens TAFII20 mRNA for transcription factor TFIID. AK310097 - Homo sapiens cDNA, FLJ17139. JD389680 - Sequence 370704 from Patent EP1572962. JD089939 - Sequence 70963 from Patent EP1572962. JD555072 - Sequence 536096 from Patent EP1572962. JD398348 - Sequence 379372 from Patent EP1572962. D50544 - Homo sapiens TFIID subunit p22 mRNA, complete cds. JD540432 - Sequence 521456 from Patent EP1572962. BT007031 - Homo sapiens TAF12 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 20kDa mRNA, complete cds. KJ892246 - Synthetic construct Homo sapiens clone ccsbBroadEn_01640 TAF12 gene, encodes complete protein. DQ890580 - Synthetic construct clone IMAGE:100003210; FLH164112.01X; RZPDo839C10162D TAF12 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 20kDa (TAF12) gene, encodes complete protein. DQ893739 - Synthetic construct Homo sapiens clone IMAGE:100008199; FLH164108.01L; RZPDo839C10161D TAF12 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 20kDa (TAF12) gene, encodes complete protein. AB528523 - Synthetic construct DNA, clone: pF1KB7913, Homo sapiens TAF12 gene for TAF12 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 20kDa, without stop codon, in Flexi system. JD477768 - Sequence 458792 from Patent EP1572962. JD563012 - Sequence 544036 from Patent EP1572962.
Biochemical and Signaling Pathways
Reactome (by CSHL, EBI, and GO)
Protein Q16514 (Reactome details) participates in the following event(s):
R-HSA-109636 Recognition and Binding of Core Promoter Elements by TFIID R-HSA-73946 Abortive initiation R-HSA-75856 Abortive Initiation Before Second Transition R-HSA-75873 Addition of Nucleotides 5 through 9 on the growing Transcript R-HSA-167136 Addition of nucleotides 5 through 9 on the growing HIV-1 transcript R-HSA-167474 Abortive HIV-1 Initiation Before Second Transition R-HSA-167477 Abortive HIV-1 initiation after formation of the first phosphodiester bond R-HSA-75866 Nucleophillic Attack by 3'-hydroxyl Oxygen of nascent transcript on the Alpha Phosphate of NTP R-HSA-75861 NTP Binds Active Site of RNA Polymerase II R-HSA-75864 Newly Formed Phosphodiester Bond Stabilized and PPi Released R-HSA-109637 Binding of TFIIA and TFIIB to the pol II promoter:TFIID complex R-HSA-75862 Fall Back to Closed Pre-initiation Complex R-HSA-75949 RNA Polymerase II Promoter Opening: First Transition R-HSA-167134 Newly formed phosphodiester bond stabilized and PPi released R-HSA-167130 Nucleophillic attack by 3'-hydroxyl oxygen of nascent HIV-1 transcript on the Alpha phosphate of NTP R-HSA-167118 NTP binds active site of RNA Polymerase II in HIV-1 open pre-initiation complex R-HSA-167484 Fall Back to Closed Pre-initiation Complex R-HSA-167097 HIV Promoter Opening: First Transition R-HSA-6805399 TAF1 phosphorylates TP53 R-HSA-109638 Recruitment of RNA Polymerase II Holoenzyme by TFIIF to the pol II promoter:TFIID:TFIIA:TFIIB complex R-HSA-3301237 KAT2 complexes acetylate histone H3 R-HSA-75095 Binding of TFIIE to the growing preinitiation complex R-HSA-109639 Formation of the closed pre-initiation complex R-HSA-73779 RNA Polymerase II Transcription Pre-Initiation And Promoter Opening R-HSA-674695 RNA Polymerase II Pre-transcription Events R-HSA-73776 RNA Polymerase II Promoter Escape R-HSA-167162 RNA Polymerase II HIV Promoter Escape R-HSA-167161 HIV Transcription Initiation R-HSA-75953 RNA Polymerase II Transcription Initiation R-HSA-76042 RNA Polymerase II Transcription Initiation And Promoter Clearance R-HSA-167172 Transcription of the HIV genome R-HSA-73857 RNA Polymerase II Transcription R-HSA-6804756 Regulation of TP53 Activity through Phosphorylation R-HSA-162599 Late Phase of HIV Life Cycle R-HSA-74160 Gene expression (Transcription) R-HSA-5633007 Regulation of TP53 Activity R-HSA-3214847 HATs acetylate histones R-HSA-162587 HIV Life Cycle R-HSA-3700989 Transcriptional Regulation by TP53 R-HSA-3247509 Chromatin modifying enzymes R-HSA-162906 HIV Infection R-HSA-212436 Generic Transcription Pathway R-HSA-4839726 Chromatin organization R-HSA-5663205 Infectious disease R-HSA-1643685 Disease
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Sequence and Links to Tools and Databases 
Common Gene Haplotype Alleles 
