Human Gene TAPBP (ENST00000434618.7_10) from GENCODE V47lift37
  Description: TAP binding protein, transcript variant 1 (from RefSeq NM_003190.5)
Gencode Transcript: ENST00000434618.7_10
Gencode Gene: ENSG00000231925.14_16
Transcript (Including UTRs)
   Position: hg19 chr6:33,267,471-33,281,855 Size: 14,385 Total Exon Count: 8 Strand: -
Coding Region
   Position: hg19 chr6:33,269,537-33,281,818 Size: 12,282 Coding Exon Count: 8 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
Gene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther Species
GO AnnotationsmRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr6:33,267,471-33,281,855)mRNA (may differ from genome)Protein (448 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMalacardsMGIOMIMPubMedReactome
UniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: TPSN_HUMAN
DESCRIPTION: RecName: Full=Tapasin; Short=TPN; Short=TPSN; AltName: Full=NGS-17; AltName: Full=TAP-associated protein; AltName: Full=TAP-binding protein; Flags: Precursor;
FUNCTION: Involved in the association of MHC class I with transporter associated with antigen processing (TAP) and in the assembly of MHC class I with peptide (peptide loading).
SUBUNIT: Heterodimer with PDIA3; disulfide-linked. Interacts with TAP1 and is thus a subunit of the TAP complex, also known as the peptide loading complex (PLC). Interaction with TAP1 is TAP2- independent and is required for efficient peptide-TAP interaction. Obligatory mediator for the interaction between newly assembled MHC class I molecules, clareticulin, PDIA3 and TAP. Up to 4 MHC class I/tapasin complexes bind to 1 TAP.
INTERACTION: Q03518:TAP1; NbExp=7; IntAct=EBI-874801, EBI-747259; Q03519:TAP2; NbExp=3; IntAct=EBI-874801, EBI-780781;
SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Single-pass type I membrane protein (Probable).
TISSUE SPECIFICITY: Neutrophils, mostly in fully differentiated cells.
DOMAIN: The N-terminus is required for efficient association with MHC class I molecule and for a stable interaction between MHC I and calreticulin. Binding to TAP is mediated by the C-terminus region.
POLYMORPHISM: The 2 alleles of TAPBP; TAPBP*01 (Tapasin*01) (shown here) and TAPBP*02 (Tapasin*02); are in linkage disequilibria with the HLA-DRB1 locus in a Japanese population.
SIMILARITY: Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
SEQUENCE CAUTION: Sequence=AAD32924.2; Type=Miscellaneous discrepancy; Note=Probable cloning artifact;
WEB RESOURCE: Name=TAPBPbase; Note=TAPBP mutation db; URL="http://bioinf.uta.fi/TAPBPbase/";

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: TAPBP
Diseases sorted by gene-association score: bare lymphocyte syndrome, type i* (926), immunodeficiency by defective expression of hla class 1* (750), maxillary sinus squamous cell carcinoma (23), alcohol-induced mental disorder (16), orbital melanoma (10), spondyloarthropathy 1 (8), alcoholic psychosis (8), suppurative cholangitis (8), maxillary sinus cancer (7), paranasal sinus cancer (7)
* = Manually curated disease association

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 94.97 RPKM in Spleen
Total median expression: 1620.81 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -8.1037-0.219 Picture PostScript Text
3' UTR -670.302066-0.324 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR007110 - Ig-like
IPR013783 - Ig-like_fold
IPR003597 - Ig_C1-set
IPR008056 - Tapasin

Pfam Domains:
PF07654 - Immunoglobulin C1-set domain

SCOP Domains:
48726 - Immunoglobulin

Protein Data Bank (PDB) 3-D Structure
MuPIT help
3F8U - X-ray MuPIT


ModBase Predicted Comparative 3D Structure on O15533
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
Gene SorterGene Sorter    
 RGD    
      
      

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0005515 protein binding
GO:0015433 peptide antigen-transporting ATPase activity
GO:0042288 MHC class I protein binding
GO:0042605 peptide antigen binding
GO:0046978 TAP1 binding
GO:0046979 TAP2 binding
GO:0051082 unfolded protein binding

Biological Process:
GO:0002474 antigen processing and presentation of peptide antigen via MHC class I
GO:0002479 antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent
GO:0006890 retrograde vesicle-mediated transport, Golgi to ER
GO:0006955 immune response
GO:0010468 regulation of gene expression
GO:0019885 antigen processing and presentation of endogenous peptide antigen via MHC class I
GO:0042886 amide transport
GO:0050823 peptide antigen stabilization
GO:0061635 regulation of protein complex stability
GO:0065003 macromolecular complex assembly
GO:1990668 vesicle fusion with endoplasmic reticulum-Golgi intermediate compartment (ERGIC) membrane

Cellular Component:
GO:0000139 Golgi membrane
GO:0005783 endoplasmic reticulum
GO:0005789 endoplasmic reticulum membrane
GO:0016020 membrane
GO:0016021 integral component of membrane
GO:0030670 phagocytic vesicle membrane
GO:0033116 endoplasmic reticulum-Golgi intermediate compartment membrane
GO:0042824 MHC class I peptide loading complex
GO:0071556 integral component of lumenal side of endoplasmic reticulum membrane
GO:0042825 TAP complex


-  Descriptions from all associated GenBank mRNAs
  AY007154 - Homo sapiens clone CDABP0094 mRNA sequence.
AF029750 - Homo sapiens tapasin (NGS-17) mRNA, complete cds.
BC064966 - Homo sapiens TAP binding protein (tapasin), mRNA (cDNA clone IMAGE:6149251), complete cds.
JD454437 - Sequence 435461 from Patent EP1572962.
JD161992 - Sequence 143016 from Patent EP1572962.
JD293857 - Sequence 274881 from Patent EP1572962.
JD416356 - Sequence 397380 from Patent EP1572962.
JD150562 - Sequence 131586 from Patent EP1572962.
JD318428 - Sequence 299452 from Patent EP1572962.
JD506135 - Sequence 487159 from Patent EP1572962.
JD215308 - Sequence 196332 from Patent EP1572962.
JD397811 - Sequence 378835 from Patent EP1572962.
JD163687 - Sequence 144711 from Patent EP1572962.
JD072909 - Sequence 53933 from Patent EP1572962.
JD212812 - Sequence 193836 from Patent EP1572962.
JD239825 - Sequence 220849 from Patent EP1572962.
JD346072 - Sequence 327096 from Patent EP1572962.
JD414717 - Sequence 395741 from Patent EP1572962.
JD476685 - Sequence 457709 from Patent EP1572962.
JD256517 - Sequence 237541 from Patent EP1572962.
JD503731 - Sequence 484755 from Patent EP1572962.
JD488168 - Sequence 469192 from Patent EP1572962.
JD076624 - Sequence 57648 from Patent EP1572962.
JD505667 - Sequence 486691 from Patent EP1572962.
JD069873 - Sequence 50897 from Patent EP1572962.
JD213260 - Sequence 194284 from Patent EP1572962.
JD213063 - Sequence 194087 from Patent EP1572962.
JD407639 - Sequence 388663 from Patent EP1572962.
JD308001 - Sequence 289025 from Patent EP1572962.
JD276737 - Sequence 257761 from Patent EP1572962.
JD177611 - Sequence 158635 from Patent EP1572962.
JD107472 - Sequence 88496 from Patent EP1572962.
JD505691 - Sequence 486715 from Patent EP1572962.
JD070333 - Sequence 51357 from Patent EP1572962.
JD070334 - Sequence 51358 from Patent EP1572962.
JD514144 - Sequence 495168 from Patent EP1572962.
JD070335 - Sequence 51359 from Patent EP1572962.
JD514145 - Sequence 495169 from Patent EP1572962.
JD070336 - Sequence 51360 from Patent EP1572962.
JD514146 - Sequence 495170 from Patent EP1572962.
JD220902 - Sequence 201926 from Patent EP1572962.
JD426737 - Sequence 407761 from Patent EP1572962.
JD153938 - Sequence 134962 from Patent EP1572962.
JD326099 - Sequence 307123 from Patent EP1572962.
JD413694 - Sequence 394718 from Patent EP1572962.
JD153937 - Sequence 134961 from Patent EP1572962.
BC010279 - Homo sapiens cDNA clone IMAGE:3047913, **** WARNING: chimeric clone ****.
JD148804 - Sequence 129828 from Patent EP1572962.
Y13582 - Homo sapiens mRNA for TAP-associated protein.
AF009510 - Homo sapiens tapasin mRNA, complete cds.
JD162572 - Sequence 143596 from Patent EP1572962.
JD539330 - Sequence 520354 from Patent EP1572962.
AB010639 - Homo sapiens mRNA for Tapasin*02, complete cds.
BC080574 - Homo sapiens TAP binding protein (tapasin), mRNA (cDNA clone MGC:99465 IMAGE:6572933), complete cds.
AF067286 - Homo sapiens tapasinas (tapasin) mRNA, alternatively spliced, complete cds.
HQ447577 - Synthetic construct Homo sapiens clone IMAGE:100070919; CCSB015013_04 TAP binding protein (tapasin) (TAPBP) gene, encodes complete protein.
EU693375 - Homo sapiens tapasin (TAPBP) mRNA, complete cds, alternatively spliced.
JD548352 - Sequence 529376 from Patent EP1572962.
JD336939 - Sequence 317963 from Patent EP1572962.
JD384579 - Sequence 365603 from Patent EP1572962.
AF314222 - Homo sapiens tapasin mRNA, complete cds, alternatively spliced.
JD421854 - Sequence 402878 from Patent EP1572962.
JD484868 - Sequence 465892 from Patent EP1572962.
JD422684 - Sequence 403708 from Patent EP1572962.
JD136554 - Sequence 117578 from Patent EP1572962.
JD384947 - Sequence 365971 from Patent EP1572962.
JD527591 - Sequence 508615 from Patent EP1572962.
JD192699 - Sequence 173723 from Patent EP1572962.
JD476048 - Sequence 457072 from Patent EP1572962.
JD044939 - Sequence 25963 from Patent EP1572962.
JD473753 - Sequence 454777 from Patent EP1572962.
CU687344 - Synthetic construct Homo sapiens gateway clone IMAGE:100023130 5' read TAPBP mRNA.
AK297903 - Homo sapiens cDNA FLJ60731 complete cds, highly similar to Tapasin precursor.
LF210788 - JP 2014500723-A/18291: Polycomb-Associated Non-Coding RNAs.
JD167792 - Sequence 148816 from Patent EP1572962.
LF341208 - JP 2014500723-A/148711: Polycomb-Associated Non-Coding RNAs.
MA446365 - JP 2018138019-A/18291: Polycomb-Associated Non-Coding RNAs.
MA576785 - JP 2018138019-A/148711: Polycomb-Associated Non-Coding RNAs.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein O15533 (Reactome details) participates in the following event(s):

R-HSA-983142 Formation of peptide loading complex (PLC)
R-HSA-8863914 Transport of SEC22B, TAP and PLC from ER to ERGIC
R-HSA-983161 Dissociation of the Antigenic peptide:MHC:B2M peptide loading complex
R-HSA-8951595 CALR, TAP, TAPBP dissociate from SEC22B:STX4
R-HSA-8863858 SEC22B, CALR, STX4, TAP and TAPBP bind
R-HSA-8951499 Loading of antigenic peptides on to class I MHC
R-HSA-983170 Antigen Presentation: Folding, assembly and peptide loading of class I MHC
R-HSA-1236974 ER-Phagosome pathway
R-HSA-983169 Class I MHC mediated antigen processing & presentation
R-HSA-1236975 Antigen processing-Cross presentation
R-HSA-1280218 Adaptive Immune System
R-HSA-168256 Immune System

-  Other Names for This Gene
  Alternate Gene Symbols: A2AB91, A2ABC0, B0V003, B0V0A6, B2ZUA4, E9PGM2, ENST00000434618.1, ENST00000434618.2, ENST00000434618.3, ENST00000434618.4, ENST00000434618.5, ENST00000434618.6, NGS17, NM_003190, O15210, O15272, O15533, Q5STJ8, Q5STK6, Q5STQ5, Q5STQ6, Q66K65, Q96KK7, Q9HAN8, Q9UEE0, Q9UEE4, Q9UIZ6, Q9Y6K2, TAPA, TAPBP , TPSN_HUMAN, uc320dcu.1, uc320dcu.2
UCSC ID: ENST00000434618.7_10
RefSeq Accession: NM_003190.5
Protein: O15533 (aka TPSN_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.