ID:TPSN_HUMAN DESCRIPTION: RecName: Full=Tapasin; Short=TPN; Short=TPSN; AltName: Full=NGS-17; AltName: Full=TAP-associated protein; AltName: Full=TAP-binding protein; Flags: Precursor; FUNCTION: Involved in the association of MHC class I with transporter associated with antigen processing (TAP) and in the assembly of MHC class I with peptide (peptide loading). SUBUNIT: Heterodimer with PDIA3; disulfide-linked. Interacts with TAP1 and is thus a subunit of the TAP complex, also known as the peptide loading complex (PLC). Interaction with TAP1 is TAP2- independent and is required for efficient peptide-TAP interaction. Obligatory mediator for the interaction between newly assembled MHC class I molecules, clareticulin, PDIA3 and TAP. Up to 4 MHC class I/tapasin complexes bind to 1 TAP. INTERACTION: Q03518:TAP1; NbExp=7; IntAct=EBI-874801, EBI-747259; Q03519:TAP2; NbExp=3; IntAct=EBI-874801, EBI-780781; SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Single-pass type I membrane protein (Probable). TISSUE SPECIFICITY: Neutrophils, mostly in fully differentiated cells. DOMAIN: The N-terminus is required for efficient association with MHC class I molecule and for a stable interaction between MHC I and calreticulin. Binding to TAP is mediated by the C-terminus region. POLYMORPHISM: The 2 alleles of TAPBP; TAPBP*01 (Tapasin*01) (shown here) and TAPBP*02 (Tapasin*02); are in linkage disequilibria with the HLA-DRB1 locus in a Japanese population. SIMILARITY: Contains 1 Ig-like C1-type (immunoglobulin-like) domain. SEQUENCE CAUTION: Sequence=AAD32924.2; Type=Miscellaneous discrepancy; Note=Probable cloning artifact; WEB RESOURCE: Name=TAPBPbase; Note=TAPBP mutation db; URL="http://bioinf.uta.fi/TAPBPbase/";
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on O15533
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
Biological Process: GO:0002474 antigen processing and presentation of peptide antigen via MHC class I GO:0002479 antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent GO:0006890 retrograde vesicle-mediated transport, Golgi to ER GO:0006955 immune response GO:0010468 regulation of gene expression GO:0019885 antigen processing and presentation of endogenous peptide antigen via MHC class I GO:0042886 amide transport GO:0050823 peptide antigen stabilization GO:0061635 regulation of protein complex stability GO:0065003 macromolecular complex assembly GO:1990668 vesicle fusion with endoplasmic reticulum-Golgi intermediate compartment (ERGIC) membrane
Cellular Component: GO:0000139 Golgi membrane GO:0005783 endoplasmic reticulum GO:0005789 endoplasmic reticulum membrane GO:0016020 membrane GO:0016021 integral component of membrane GO:0030670 phagocytic vesicle membrane GO:0033116 endoplasmic reticulum-Golgi intermediate compartment membrane GO:0042824 MHC class I peptide loading complex GO:0071556 integral component of lumenal side of endoplasmic reticulum membrane GO:0042825 TAP complex
Descriptions from all associated GenBank mRNAs
AY007154 - Homo sapiens clone CDABP0094 mRNA sequence. AF029750 - Homo sapiens tapasin (NGS-17) mRNA, complete cds. BC064966 - Homo sapiens TAP binding protein (tapasin), mRNA (cDNA clone IMAGE:6149251), complete cds. JD454437 - Sequence 435461 from Patent EP1572962. JD161992 - Sequence 143016 from Patent EP1572962. JD293857 - Sequence 274881 from Patent EP1572962. JD416356 - Sequence 397380 from Patent EP1572962. JD150562 - Sequence 131586 from Patent EP1572962. JD318428 - Sequence 299452 from Patent EP1572962. JD506135 - Sequence 487159 from Patent EP1572962. JD215308 - Sequence 196332 from Patent EP1572962. JD397811 - Sequence 378835 from Patent EP1572962. JD163687 - Sequence 144711 from Patent EP1572962. JD072909 - Sequence 53933 from Patent EP1572962. JD212812 - Sequence 193836 from Patent EP1572962. JD239825 - Sequence 220849 from Patent EP1572962. JD346072 - Sequence 327096 from Patent EP1572962. JD414717 - Sequence 395741 from Patent EP1572962. JD476685 - Sequence 457709 from Patent EP1572962. JD256517 - Sequence 237541 from Patent EP1572962. JD503731 - Sequence 484755 from Patent EP1572962. JD488168 - Sequence 469192 from Patent EP1572962. JD076624 - Sequence 57648 from Patent EP1572962. JD505667 - Sequence 486691 from Patent EP1572962. JD069873 - Sequence 50897 from Patent EP1572962. JD213260 - Sequence 194284 from Patent EP1572962. JD213063 - Sequence 194087 from Patent EP1572962. JD407639 - Sequence 388663 from Patent EP1572962. JD308001 - Sequence 289025 from Patent EP1572962. JD276737 - Sequence 257761 from Patent EP1572962. JD177611 - Sequence 158635 from Patent EP1572962. JD107472 - Sequence 88496 from Patent EP1572962. JD505691 - Sequence 486715 from Patent EP1572962. JD070333 - Sequence 51357 from Patent EP1572962. JD070334 - Sequence 51358 from Patent EP1572962. JD514144 - Sequence 495168 from Patent EP1572962. JD070335 - Sequence 51359 from Patent EP1572962. JD514145 - Sequence 495169 from Patent EP1572962. JD070336 - Sequence 51360 from Patent EP1572962. JD514146 - Sequence 495170 from Patent EP1572962. JD220902 - Sequence 201926 from Patent EP1572962. JD426737 - Sequence 407761 from Patent EP1572962. JD153938 - Sequence 134962 from Patent EP1572962. JD326099 - Sequence 307123 from Patent EP1572962. JD413694 - Sequence 394718 from Patent EP1572962. JD153937 - Sequence 134961 from Patent EP1572962. BC010279 - Homo sapiens cDNA clone IMAGE:3047913, **** WARNING: chimeric clone ****. JD148804 - Sequence 129828 from Patent EP1572962. Y13582 - Homo sapiens mRNA for TAP-associated protein. AF009510 - Homo sapiens tapasin mRNA, complete cds. JD162572 - Sequence 143596 from Patent EP1572962. JD539330 - Sequence 520354 from Patent EP1572962. AB010639 - Homo sapiens mRNA for Tapasin*02, complete cds. BC080574 - Homo sapiens TAP binding protein (tapasin), mRNA (cDNA clone MGC:99465 IMAGE:6572933), complete cds. AF067286 - Homo sapiens tapasinas (tapasin) mRNA, alternatively spliced, complete cds. HQ447577 - Synthetic construct Homo sapiens clone IMAGE:100070919; CCSB015013_04 TAP binding protein (tapasin) (TAPBP) gene, encodes complete protein. EU693375 - Homo sapiens tapasin (TAPBP) mRNA, complete cds, alternatively spliced. JD548352 - Sequence 529376 from Patent EP1572962. JD336939 - Sequence 317963 from Patent EP1572962. JD384579 - Sequence 365603 from Patent EP1572962. AF314222 - Homo sapiens tapasin mRNA, complete cds, alternatively spliced. JD421854 - Sequence 402878 from Patent EP1572962. JD484868 - Sequence 465892 from Patent EP1572962. JD422684 - Sequence 403708 from Patent EP1572962. JD136554 - Sequence 117578 from Patent EP1572962. JD384947 - Sequence 365971 from Patent EP1572962. JD527591 - Sequence 508615 from Patent EP1572962. JD192699 - Sequence 173723 from Patent EP1572962. JD476048 - Sequence 457072 from Patent EP1572962. JD044939 - Sequence 25963 from Patent EP1572962. JD473753 - Sequence 454777 from Patent EP1572962. CU687344 - Synthetic construct Homo sapiens gateway clone IMAGE:100023130 5' read TAPBP mRNA. AK297903 - Homo sapiens cDNA FLJ60731 complete cds, highly similar to Tapasin precursor. LF210788 - JP 2014500723-A/18291: Polycomb-Associated Non-Coding RNAs. JD167792 - Sequence 148816 from Patent EP1572962. LF341208 - JP 2014500723-A/148711: Polycomb-Associated Non-Coding RNAs. MA446365 - JP 2018138019-A/18291: Polycomb-Associated Non-Coding RNAs. MA576785 - JP 2018138019-A/148711: Polycomb-Associated Non-Coding RNAs.
Biochemical and Signaling Pathways
Reactome (by CSHL, EBI, and GO)
Protein O15533 (Reactome details) participates in the following event(s):
R-HSA-983142 Formation of peptide loading complex (PLC) R-HSA-8863914 Transport of SEC22B, TAP and PLC from ER to ERGIC R-HSA-983161 Dissociation of the Antigenic peptide:MHC:B2M peptide loading complex R-HSA-8951595 CALR, TAP, TAPBP dissociate from SEC22B:STX4 R-HSA-8863858 SEC22B, CALR, STX4, TAP and TAPBP bind R-HSA-8951499 Loading of antigenic peptides on to class I MHC R-HSA-983170 Antigen Presentation: Folding, assembly and peptide loading of class I MHC R-HSA-1236974 ER-Phagosome pathway R-HSA-983169 Class I MHC mediated antigen processing & presentation R-HSA-1236975 Antigen processing-Cross presentation R-HSA-1280218 Adaptive Immune System R-HSA-168256 Immune System
US Server
Sequence and Links to Tools and Databases 
Common Gene Haplotype Alleles 
