Human Gene TEAD2 (ENST00000593945.6_12) from GENCODE V47lift37
  Description: TEA domain transcription factor 2, transcript variant 3 (from RefSeq NM_001256660.2)
Gencode Transcript: ENST00000593945.6_12
Gencode Gene: ENSG00000074219.14_18
Transcript (Including UTRs)
   Position: hg19 chr19:49,843,852-49,865,673 Size: 21,822 Total Exon Count: 13 Strand: -
Coding Region
   Position: hg19 chr19:49,844,581-49,863,332 Size: 18,752 Coding Exon Count: 12 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr19:49,843,852-49,865,673)mRNA (may differ from genome)Protein (451 aa)
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HGNCMGIOMIMPubMedReactomeUniProtKB
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-  Comments and Description Text from UniProtKB
  ID: TEAD2_HUMAN
DESCRIPTION: RecName: Full=Transcriptional enhancer factor TEF-4; AltName: Full=TEA domain family member 2; Short=TEAD-2;
FUNCTION: Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds to the SPH and GT-IIC 'enhansons' (5'- GTGGAATGT-3'). May be involved in the gene regulation of neural development. Binds to the M-CAT motif.
SUBUNIT: Interacts with YAP1 and WWTR1/TAZ.
SUBCELLULAR LOCATION: Nucleus.
SIMILARITY: Contains 1 TEA DNA-binding domain.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 19.77 RPKM in Testis
Total median expression: 418.76 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -45.5090-0.506 Picture PostScript Text
3' UTR -239.30729-0.328 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR000818 - TEA/ATTS
IPR016361 - TEF

Pfam Domains:
PF01285 - TEA/ATTS domain
PF17725 - YAP binding domain

SCOP Domains:
46689 - Homeodomain-like

Protein Data Bank (PDB) 3-D Structure
MuPIT help
3L15 - X-ray MuPIT


ModBase Predicted Comparative 3D Structure on Q15562
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000981 RNA polymerase II transcription factor activity, sequence-specific DNA binding
GO:0001085 RNA polymerase II transcription factor binding
GO:0001134 transcription factor activity, transcription factor recruiting
GO:0001223 transcription coactivator binding
GO:0003677 DNA binding
GO:0003700 transcription factor activity, sequence-specific DNA binding
GO:0003705 transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding
GO:0005515 protein binding
GO:0043565 sequence-specific DNA binding
GO:0044212 transcription regulatory region DNA binding
GO:0046982 protein heterodimerization activity
GO:0097718 disordered domain specific binding

Biological Process:
GO:0001570 vasculogenesis
GO:0001843 neural tube closure
GO:0003143 embryonic heart tube morphogenesis
GO:0006351 transcription, DNA-templated
GO:0006355 regulation of transcription, DNA-templated
GO:0006367 transcription initiation from RNA polymerase II promoter
GO:0030903 notochord development
GO:0035329 hippo signaling
GO:0045893 positive regulation of transcription, DNA-templated
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0048339 paraxial mesoderm development
GO:0048368 lateral mesoderm development
GO:0048568 embryonic organ development
GO:0060548 negative regulation of cell death
GO:0065003 macromolecular complex assembly
GO:0071300 cellular response to retinoic acid
GO:2000736 regulation of stem cell differentiation

Cellular Component:
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005667 transcription factor complex
GO:0005829 cytosol
GO:0043231 intracellular membrane-bounded organelle
GO:0071149 TEAD-2-YAP complex


-  Descriptions from all associated GenBank mRNAs
  BC018803 - Homo sapiens TEA domain family member 2, mRNA (cDNA clone IMAGE:4634096).
AL832240 - Homo sapiens mRNA; cDNA DKFZp686I2377 (from clone DKFZp686I2377).
BC051301 - Homo sapiens TEA domain family member 2, mRNA (cDNA clone MGC:59867 IMAGE:4843913), complete cds.
BC007556 - Homo sapiens TEA domain family member 2, mRNA (cDNA clone MGC:15481 IMAGE:2967735), complete cds.
AK093236 - Homo sapiens cDNA FLJ35917 fis, clone TESTI2010409, highly similar to TRANSCRIPTIONAL ENHANCER FACTOR TEF-4.
AX748004 - Sequence 1529 from Patent EP1308459.
JD261750 - Sequence 242774 from Patent EP1572962.
JD452741 - Sequence 433765 from Patent EP1572962.
JD527874 - Sequence 508898 from Patent EP1572962.
JD167160 - Sequence 148184 from Patent EP1572962.
JD127297 - Sequence 108321 from Patent EP1572962.
AK290736 - Homo sapiens cDNA FLJ77886 complete cds, highly similar to Homo sapiens TEA domain family member 2, mRNA.
JD132800 - Sequence 113824 from Patent EP1572962.
JD566605 - Sequence 547629 from Patent EP1572962.
JD433107 - Sequence 414131 from Patent EP1572962.
JD098887 - Sequence 79911 from Patent EP1572962.
AK300241 - Homo sapiens cDNA FLJ57003 complete cds, highly similar to Transcriptional enhancer factor TEF-4.
JD321682 - Sequence 302706 from Patent EP1572962.
JD108492 - Sequence 89516 from Patent EP1572962.
JD051067 - Sequence 32091 from Patent EP1572962.
KJ892539 - Synthetic construct Homo sapiens clone ccsbBroadEn_01933 TEAD2 gene, encodes complete protein.
DQ893163 - Synthetic construct clone IMAGE:100005793; FLH194519.01X; RZPDo839C0380D TEA domain family member 2 (TEAD2) gene, encodes complete protein.
DQ896460 - Synthetic construct Homo sapiens clone IMAGE:100010920; FLH194515.01L; RZPDo839C0370D TEA domain family member 2 (TEAD2) gene, encodes complete protein.
AB464291 - Synthetic construct DNA, clone: pF1KB7767, Homo sapiens TEAD2 gene for TEA domain family member 2, without stop codon, in Flexi system.
JD021001 - Sequence 2025 from Patent EP1572962.
JD030238 - Sequence 11262 from Patent EP1572962.
X94440 - H.sapiens mRNA for TEF-4 protein.
CU675701 - Synthetic construct Homo sapiens gateway clone IMAGE:100017289 5' read TEAD2 mRNA.
JD129613 - Sequence 110637 from Patent EP1572962.
JD405854 - Sequence 386878 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q15562 (Reactome details) participates in the following event(s):

R-HSA-8871260 YAP1 binds TEAD2
R-HSA-2032781 WWTR1 (TAZ) binds TEAD
R-HSA-8951695 TEADs:YAP1 binds CTGF gene
R-HSA-8951676 RUNX3 binds TEADs and YAP1
R-HSA-2032785 YAP1- and WWTR1 (TAZ)-stimulated gene expression
R-HSA-212436 Generic Transcription Pathway
R-HSA-8951671 RUNX3 regulates YAP1-mediated transcription
R-HSA-73857 RNA Polymerase II Transcription
R-HSA-8878159 Transcriptional regulation by RUNX3
R-HSA-74160 Gene expression (Transcription)

-  Other Names for This Gene
  Alternate Gene Symbols: B4DTJ6, ENST00000593945.1, ENST00000593945.2, ENST00000593945.3, ENST00000593945.4, ENST00000593945.5, M0R1T9, NM_001256660, Q15562, Q8NA25, Q96IG3, TEAD2_HUMAN, TEF4, uc326zfq.1, uc326zfq.2
UCSC ID: ENST00000593945.6_12
RefSeq Accession: NM_001256660.2
Protein: Q15562 (aka TEAD2_HUMAN or TEA2_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.