Human Gene TFDP3 (ENST00000310125.5_6) from GENCODE V47lift37
  Description: transcription factor Dp family member 3 (from RefSeq NM_016521.3)
Gencode Transcript: ENST00000310125.5_6
Gencode Gene: ENSG00000183434.10_9
Transcript (Including UTRs)
   Position: hg19 chrX:132,350,690-132,352,382 Size: 1,693 Total Exon Count: 1 Strand: -
Coding Region
   Position: hg19 chrX:132,351,070-132,352,287 Size: 1,218 Coding Exon Count: 1 

Page IndexSequence and LinksUniProtKB CommentsPrimersGene AllelesRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
Other NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chrX:132,350,690-132,352,382)mRNA (may differ from genome)Protein (405 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGeneCardsHGNC
MGIOMIMPubMedUniProtKBBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: TFDP3_HUMAN
DESCRIPTION: RecName: Full=Transcription factor Dp family member 3; AltName: Full=Cancer/testis antigen 30; Short=CT30; AltName: Full=Hepatocellular carcinoma-associated antigen 661;
FUNCTION: Competitive inhibitor of E2F-mediated transactivation activity. Impairs E2F-mediated cell-cycle progression from G(1) to S phase.
SUBUNIT: Heterodimer: with E2F family members. TFDP3/E2F heterodimers do not bind DNA and repress E2F-dependent transcriptional activity.
SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note=Translocates to the nucleus on heterodimerization with E2F family members.
TISSUE SPECIFICITY: Predominantly expressed in testis. Low level of expression in pancreas. Highly expressed in ovarian and colon cancer cell lines.
INDUCTION: In response to DNA damage.
DOMAIN: The potential DNA-binding domain differs in sequence from that of other DP family members and can not bind DNA.
SIMILARITY: Belongs to the E2F/DP family.
SEQUENCE CAUTION: Sequence=AAF37562.2; Type=Erroneous initiation; Note=Translation N-terminally extended;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


+  Common Gene Haplotype Alleles
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-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 4.45 RPKM in Testis
Total median expression: 4.49 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
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-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -16.5095-0.174 Picture PostScript Text
3' UTR -75.50380-0.199 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR003316 - E2F_TDP
IPR014889 - Transc_factor_DP_C
IPR016556 - Transcription_factor_DP_subgr
IPR015648 - Transcrpt_fac_DP
IPR011991 - WHTH_trsnscrt_rep_DNA-bd

Pfam Domains:
PF02319 - E2F/DP family winged-helix DNA-binding domain
PF08781 - Transcription factor DP

SCOP Domains:
46785 - "Winged helix" DNA-binding domain
144074 - E2F-DP heterodimerization region

ModBase Predicted Comparative 3D Structure on Q5H9I0
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000981 RNA polymerase II transcription factor activity, sequence-specific DNA binding
GO:0003677 DNA binding
GO:0003700 transcription factor activity, sequence-specific DNA binding
GO:0046982 protein heterodimerization activity

Biological Process:
GO:0000082 G1/S transition of mitotic cell cycle
GO:0006351 transcription, DNA-templated
GO:0006355 regulation of transcription, DNA-templated
GO:0006357 regulation of transcription from RNA polymerase II promoter
GO:0006974 cellular response to DNA damage stimulus
GO:0007049 cell cycle

Cellular Component:
GO:0005634 nucleus
GO:0005667 transcription factor complex
GO:0005737 cytoplasm


-  Descriptions from all associated GenBank mRNAs
  AF219119 - Homo sapiens E2F-like protein (HCA661) mRNA, complete cds.
JD284369 - Sequence 265393 from Patent EP1572962.
BC117336 - Homo sapiens transcription factor Dp family, member 3, mRNA (cDNA clone IMAGE:40125887).
BC126361 - Homo sapiens transcription factor Dp family, member 3, mRNA (cDNA clone IMAGE:8992077).
BC074762 - Homo sapiens transcription factor Dp family, member 3, mRNA (cDNA clone IMAGE:30915221).
KJ902575 - Synthetic construct Homo sapiens clone ccsbBroadEn_11969 TFDP3 gene, encodes complete protein.
EU446842 - Synthetic construct Homo sapiens clone IMAGE:100070120; IMAGE:100012051; FLH257463.01L transcription factor Dp family, member 3 (TFDP3) gene, encodes complete protein.
AB464736 - Synthetic construct DNA, clone: pF1KB9590, Homo sapiens TFDP3 gene for transcription factor Dp family, member 3, without stop codon, in Flexi system.
JD271211 - Sequence 252235 from Patent EP1572962.
JD334060 - Sequence 315084 from Patent EP1572962.

-  Other Names for This Gene
  Alternate Gene Symbols: DP4, ENST00000310125.1, ENST00000310125.2, ENST00000310125.3, ENST00000310125.4, HCA661, NM_016521, Q5H9I0, Q6DK49, Q9NZ54, TFDP3_HUMAN, uc317orq.1, uc317orq.2
UCSC ID: ENST00000310125.5_6
RefSeq Accession: NM_016521.3
Protein: Q5H9I0 (aka TFDP3_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.