Human Gene TFPI (ENST00000233156.9_8) from GENCODE V47lift37
  Description: tissue factor pathway inhibitor, transcript variant 1 (from RefSeq NM_006287.6)
Gencode Transcript: ENST00000233156.9_8
Gencode Gene: ENSG00000003436.16_12
Transcript (Including UTRs)
   Position: hg19 chr2:188,328,957-188,419,162 Size: 90,206 Total Exon Count: 8 Strand: -
Coding Region
   Position: hg19 chr2:188,331,663-188,368,495 Size: 36,833 Coding Exon Count: 7 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
Gene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther Species
GO AnnotationsmRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr2:188,328,957-188,419,162)mRNA (may differ from genome)Protein (304 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMalacardsMGIOMIMPubMedReactome
UniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: TFPI1_HUMAN
DESCRIPTION: RecName: Full=Tissue factor pathway inhibitor; Short=TFPI; AltName: Full=Extrinsic pathway inhibitor; Short=EPI; AltName: Full=Lipoprotein-associated coagulation inhibitor; Short=LACI; Flags: Precursor;
FUNCTION: Inhibits factor X (X(a)) directly and, in a Xa-dependent way, inhibits VIIa/tissue factor activity, presumably by forming a quaternary Xa/LACI/VIIa/TF complex. It possesses an antithrombotic action and also the ability to associate with lipoproteins in plasma.
SUBCELLULAR LOCATION: Isoform Alpha: Secreted.
SUBCELLULAR LOCATION: Isoform Beta: Microsome membrane; Lipid- anchor, GPI-anchor.
TISSUE SPECIFICITY: Mostly in endothelial cells.
DOMAIN: This inhibitor contains three inhibitory domains. The first domain interacts with VIIa and TF, the second one with Xa.
PTM: O-glycosylated.
SIMILARITY: Contains 3 BPTI/Kunitz inhibitor domains.
WEB RESOURCE: Name=Wikipedia; Note=TFPI entry; URL="http://en.wikipedia.org/wiki/TFPI";
WEB RESOURCE: Name=SeattleSNPs; URL="http://pga.gs.washington.edu/data/tfpi/";

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: TFPI
Diseases sorted by gene-association score: hemophilia (16), thrombosis (8), crescentic glomerulonephritis (6), factor x deficiency (4), hypoprothrombinemia (4)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 51.83 RPKM in Lung
Total median expression: 488.83 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -46.70238-0.196 Picture PostScript Text
3' UTR -559.402706-0.207 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR002223 - Prot_inh_Kunz-m
IPR008296 - Prot_inhib_I2_TFPI
IPR020901 - Prtase_inh_Kunz-CS

Pfam Domains:
PF00014 - Kunitz/Bovine pancreatic trypsin inhibitor domain

SCOP Domains:
57414 - Hairpin loop containing domain-like
57362 - BPTI-like

Protein Data Bank (PDB) 3-D Structure
MuPIT help
1ADZ - NMR MuPIT 1IRH - NMR MuPIT 1TFX - X-ray MuPIT 4DTG - X-ray MuPIT


ModBase Predicted Comparative 3D Structure on P10646
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologGenome BrowserNo ortholog
Gene DetailsGene Details  Gene Details 
Gene SorterGene Sorter  Gene Sorter 
 RGDEnsembl WormBase 
    Protein Sequence 
    Alignment 

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004866 endopeptidase inhibitor activity
GO:0004867 serine-type endopeptidase inhibitor activity
GO:0030414 peptidase inhibitor activity

Biological Process:
GO:0007596 blood coagulation
GO:0007598 blood coagulation, extrinsic pathway
GO:0007599 hemostasis
GO:0010466 negative regulation of peptidase activity
GO:0010951 negative regulation of endopeptidase activity
GO:0030195 negative regulation of blood coagulation
GO:0032355 response to estradiol
GO:0032496 response to lipopolysaccharide
GO:0071222 cellular response to lipopolysaccharide
GO:0071347 cellular response to interleukin-1
GO:0071383 cellular response to steroid hormone stimulus

Cellular Component:
GO:0005576 extracellular region
GO:0005615 extracellular space
GO:0005783 endoplasmic reticulum
GO:0005886 plasma membrane
GO:0005901 caveola
GO:0009986 cell surface
GO:0016020 membrane
GO:0031090 organelle membrane
GO:0031225 anchored component of membrane
GO:0043231 intracellular membrane-bounded organelle


-  Descriptions from all associated GenBank mRNAs
  LP895417 - Sequence 281 from Patent EP3253886.
J03225 - Human lipoprotein-associated coagulation inhibitor mRNA, complete cds.
LF205742 - JP 2014500723-A/13245: Polycomb-Associated Non-Coding RNAs.
E05515 - DNA sequence coding for LACI.
AK313562 - Homo sapiens cDNA, FLJ94125, highly similar to Homo sapiens tissue factor pathway inhibitor(lipoprotein-associated coagulation inhibitor) (TFPI), mRNA.
AB590507 - Synthetic construct DNA, clone: pFN21AE1792, Homo sapiens TFPI gene for tissue factor pathway inhibitor, without stop codon, in Flexi system.
BC014332 - Homo sapiens tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor), mRNA (cDNA clone IMAGE:4043939), with apparent retained intron.
AB209866 - Homo sapiens mRNA for tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor) variant protein.
AF021834 - Homo sapiens tissue factor pathway inhibitor beta (TFPIbeta) mRNA, complete cds.
BC015514 - Homo sapiens tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor), mRNA (cDNA clone MGC:9251 IMAGE:3902987), complete cds.
CU677331 - Synthetic construct Homo sapiens gateway clone IMAGE:100016891 5' read TFPI mRNA.
HQ447091 - Synthetic construct Homo sapiens clone IMAGE:100070374; CCSB000741_01 tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor) (TFPI) gene, encodes complete protein.
KJ897659 - Synthetic construct Homo sapiens clone ccsbBroadEn_07053 TFPI gene, encodes complete protein.
KR709392 - Synthetic construct Homo sapiens clone CCSBHm_00001829 TFPI (TFPI) mRNA, encodes complete protein.
MA441319 - JP 2018138019-A/13245: Polycomb-Associated Non-Coding RNAs.
AK130306 - Homo sapiens cDNA FLJ26796 fis, clone PRS05079.
LF347725 - JP 2014500723-A/155228: Polycomb-Associated Non-Coding RNAs.
LF347726 - JP 2014500723-A/155229: Polycomb-Associated Non-Coding RNAs.
LF347727 - JP 2014500723-A/155230: Polycomb-Associated Non-Coding RNAs.
LF347736 - JP 2014500723-A/155239: Polycomb-Associated Non-Coding RNAs.
LF347737 - JP 2014500723-A/155240: Polycomb-Associated Non-Coding RNAs.
LF347738 - JP 2014500723-A/155241: Polycomb-Associated Non-Coding RNAs.
MA583302 - JP 2018138019-A/155228: Polycomb-Associated Non-Coding RNAs.
MA583303 - JP 2018138019-A/155229: Polycomb-Associated Non-Coding RNAs.
MA583304 - JP 2018138019-A/155230: Polycomb-Associated Non-Coding RNAs.
MA583313 - JP 2018138019-A/155239: Polycomb-Associated Non-Coding RNAs.
MA583314 - JP 2018138019-A/155240: Polycomb-Associated Non-Coding RNAs.
MA583315 - JP 2018138019-A/155241: Polycomb-Associated Non-Coding RNAs.
LF347741 - JP 2014500723-A/155244: Polycomb-Associated Non-Coding RNAs.
LF347745 - JP 2014500723-A/155248: Polycomb-Associated Non-Coding RNAs.
MA583318 - JP 2018138019-A/155244: Polycomb-Associated Non-Coding RNAs.
MA583322 - JP 2018138019-A/155248: Polycomb-Associated Non-Coding RNAs.

-  Biochemical and Signaling Pathways
  BioCarta from NCI Cancer Genome Anatomy Project
h_extrinsicPathway - Extrinsic Prothrombin Activation Pathway
h_amiPathway - Acute Myocardial Infarction

Reactome (by CSHL, EBI, and GO)

Protein P10646 (Reactome details) participates in the following event(s):

R-HSA-140825 TFPI + TF:F7a + factor Xa -> TFPI:TF:F7a:factor Xa
R-HSA-140834 Extrinsic Pathway of Fibrin Clot Formation
R-HSA-140877 Formation of Fibrin Clot (Clotting Cascade)
R-HSA-109582 Hemostasis

-  Other Names for This Gene
  Alternate Gene Symbols: ENST00000233156.1, ENST00000233156.2, ENST00000233156.3, ENST00000233156.4, ENST00000233156.5, ENST00000233156.6, ENST00000233156.7, ENST00000233156.8, LACI, NM_006287, O95103, P10646, Q53TS4, TFPI1, TFPI1_HUMAN, uc317dwr.1, uc317dwr.2
UCSC ID: ENST00000233156.9_8
RefSeq Accession: NM_006287.6
Protein: P10646 (aka TFPI1_HUMAN or TFPI_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.