Human Gene TIE1 (ENST00000372476.8_4) from GENCODE V47lift37
  Description: tyrosine kinase with immunoglobulin like and EGF like domains 1, transcript variant 1 (from RefSeq NM_005424.5)
Gencode Transcript: ENST00000372476.8_4
Gencode Gene: ENSG00000066056.15_7
Transcript (Including UTRs)
   Position: hg19 chr1:43,766,653-43,788,779 Size: 22,127 Total Exon Count: 23 Strand: +
Coding Region
   Position: hg19 chr1:43,766,743-43,788,393 Size: 21,651 Coding Exon Count: 23 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr1:43,766,653-43,788,779)mRNA (may differ from genome)Protein (1138 aa)
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-  Comments and Description Text from UniProtKB
  ID: TIE1_HUMAN
DESCRIPTION: RecName: Full=Tyrosine-protein kinase receptor Tie-1; EC=2.7.10.1; Flags: Precursor;
FUNCTION: Transmembrane tyrosine-protein kinase that may modulate TEK/TIE2 activity and contribute to the regulation of angiogenesis.
CATALYTIC ACTIVITY: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
SUBUNIT: Heterodimer with TEK/TIE2.
INTERACTION: Q15238:PSG5; NbExp=3; IntAct=EBI-2256865, EBI-4314891;
SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein.
TISSUE SPECIFICITY: Specifically expressed in developing vascular endothelial cells.
PTM: Phosphorylated on tyrosine residues in response to ANGPT1, most likely by TEK/TIE2.
SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. Tie subfamily.
SIMILARITY: Contains 3 EGF-like domains.
SIMILARITY: Contains 3 fibronectin type-III domains.
SIMILARITY: Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
SIMILARITY: Contains 1 protein kinase domain.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 21.70 RPKM in Adipose - Visceral (Omentum)
Total median expression: 300.78 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
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-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -38.2090-0.424 Picture PostScript Text
3' UTR -101.80386-0.264 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR000742 - EG-like_dom
IPR013032 - EGF-like_CS
IPR003961 - Fibronectin_type3
IPR013783 - Ig-like_fold
IPR003599 - Ig_sub
IPR011009 - Kinase-like_dom
IPR000719 - Prot_kinase_cat_dom
IPR017441 - Protein_kinase_ATP_BS
IPR001245 - Ser-Thr/Tyr_kinase_cat_dom
IPR008266 - Tyr_kinase_AS
IPR020635 - Tyr_kinase_cat_dom

Pfam Domains:
PF00041 - Fibronectin type III domain
PF00047 - Immunoglobulin domain
PF00069 - Protein kinase domain
PF07714 - Protein tyrosine and serine/threonine kinase
PF12661 - Human growth factor-like EGF

SCOP Domains:
48726 - Immunoglobulin
49363 - Purple acid phosphatase, N-terminal domain
49265 - Fibronectin type III
56112 - Protein kinase-like (PK-like)
57196 - EGF/Laminin
57184 - Growth factor receptor domain

ModBase Predicted Comparative 3D Structure on P35590
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000166 nucleotide binding
GO:0004672 protein kinase activity
GO:0004713 protein tyrosine kinase activity
GO:0004714 transmembrane receptor protein tyrosine kinase activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0016301 kinase activity
GO:0016740 transferase activity

Biological Process:
GO:0001525 angiogenesis
GO:0001568 blood vessel development
GO:0001570 vasculogenesis
GO:0001701 in utero embryonic development
GO:0006468 protein phosphorylation
GO:0007165 signal transduction
GO:0007498 mesoderm development
GO:0016310 phosphorylation
GO:0016525 negative regulation of angiogenesis
GO:0018108 peptidyl-tyrosine phosphorylation
GO:0030336 negative regulation of cell migration
GO:0032526 response to retinoic acid
GO:0045026 plasma membrane fusion

Cellular Component:
GO:0005886 plasma membrane
GO:0005887 integral component of plasma membrane
GO:0016020 membrane
GO:0016021 integral component of membrane


-  Descriptions from all associated GenBank mRNAs
  AK307639 - Homo sapiens cDNA, FLJ97587.
BC038239 - Homo sapiens tyrosine kinase with immunoglobulin-like and EGF-like domains 1, mRNA (cDNA clone MGC:46155 IMAGE:5767075), complete cds.
AK300399 - Homo sapiens cDNA FLJ53921 complete cds, highly similar to Tyrosine-protein kinase receptor Tie-1 precursor (EC 2.7.10.1).
AK225560 - Homo sapiens mRNA for tyrosine kinase with immunoglobulin-like and EGF-like domains 1 variant, clone: KDN07127.
AF217976 - Homo sapiens clone PP1440 unknown mRNA.
AK300623 - Homo sapiens cDNA FLJ51684 complete cds, moderately similar to Tyrosine-protein kinase receptor Tie-1 precursor (EC 2.7.10.1).
A16753 - tie receptor tyrosine kinase seq ID No 3.
A16754 - tie receptor tyrosine kinase (alternatively spliced to lack one of the epidermal growth factor homology domains) seq ID No 4.
X60957 - Human tie mRNA for putative receptor tyrosine kinase.
EU826590 - Homo sapiens soluble TIE1 variant 5 (TIE) mRNA, complete cds, alternatively spliced.
AB385197 - Synthetic construct DNA, clone: pF1KB9177, Homo sapiens TIE1 gene for tyrosine-protein kinase receptor Tie-1 precursor, complete cds, without stop codon, in Flexi system.
KJ897674 - Synthetic construct Homo sapiens clone ccsbBroadEn_07068 TIE1 gene, encodes complete protein.
AK296735 - Homo sapiens cDNA FLJ52321 complete cds, highly similar to Tyrosine-protein kinase receptor Tie-1 precursor (EC 2.7.10.1).
JD194514 - Sequence 175538 from Patent EP1572962.
JD206075 - Sequence 187099 from Patent EP1572962.
EU826589 - Homo sapiens soluble TIE1 variant 4 (TIE) mRNA, complete cds, alternatively spliced.
EU826586 - Homo sapiens soluble TIE1 variant 1 (TIE) mRNA, complete cds, alternatively spliced.
CU691552 - Synthetic construct Homo sapiens gateway clone IMAGE:100020576 5' read TIE1 mRNA.
EU826587 - Homo sapiens soluble TIE1 variant 2 (TIE) mRNA, complete cds, alternatively spliced.
EU826588 - Homo sapiens soluble TIE1 variant 3 (TIE) mRNA, complete cds, alternatively spliced.
AL833389 - Homo sapiens mRNA; cDNA DKFZp667O2214 (from clone DKFZp667O2214).
JD184770 - Sequence 165794 from Patent EP1572962.
JD285360 - Sequence 266384 from Patent EP1572962.
JD435707 - Sequence 416731 from Patent EP1572962.
JD554658 - Sequence 535682 from Patent EP1572962.
JD039514 - Sequence 20538 from Patent EP1572962.
JD410481 - Sequence 391505 from Patent EP1572962.
JD415423 - Sequence 396447 from Patent EP1572962.

-  Other Names for This Gene
  Alternate Gene Symbols: B5A949, B5A950, ENST00000372476.1, ENST00000372476.2, ENST00000372476.3, ENST00000372476.4, ENST00000372476.5, ENST00000372476.6, ENST00000372476.7, NM_005424, P35590, TIE, TIE1_HUMAN, uc318jyh.1, uc318jyh.2
UCSC ID: ENST00000372476.8_4
RefSeq Accession: NM_005424.5
Protein: P35590 (aka TIE1_HUMAN)

-  Gene Model Information
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-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.